Regulation of cell substrate adhesion: effects of small galactosaminoglycan-containing proteoglycans

doi:10.1083/jcb.118.6.1523

This Article

	Full Text (PDF, 1854K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Bidanset, D. J.
	Articles by Hook, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Bidanset, D. J.
	Articles by Hook, M.


Social Bookmarking

	What's this?

ARTICLES

Regulation of cell substrate adhesion: effects of small galactosaminoglycan-containing proteoglycans

DJ Bidanset, R LeBaron, L Rosenberg, JE Murphy-Ullrich and M Hook
Department of Cell Biology, University of Alabama at Birmingham 35294.

Cell adhesion is a process which is initiated by the attachment of cells to specific sites in adhesive matrix proteins via cell surface receptors of the integrin family. This is followed by a reorganization of cytoskeletal elements which results in cell spreading and the formation of focal adhesion plaques. We have examined the effects of a class of small galactosaminoglycan-containing proteoglycans on the various stages of cell adhesion to fibronectin-coated substrates. Our results indicate that dermatan sulfate proteoglycans (DSPGs) derived from cartilage, as well as other related small proteoglycans, inhibit the initial attachment of CHO cells and rat embryo fibroblasts to substrates composed of the 105-kD cell-binding fibronectin fragment, but do not affect cell attachment to intact fibronectin. Although this effect involves binding of DSPGs to the substrate via the protein core, the intact proteoglycan is necessary for the observed activity. Isolated core proteins are inactive. The structural composition of the galactosaminoglycan chain does not appear to be functionally significant since both chondroitin sulfate and various dermatan sulfate proteoglycans of this family inhibit cell attachment to the fibronectin fragment. Neither the percentage of cells spread nor the mean area of spread cells adhering to substrates of intact fibronectin was significantly affected by the DSPGs. However, significantly fewer cells formed focal adhesions in the presence of DSPGs as compared with untreated control cells. These results suggest that the binding of small galactosaminoglycan-containing proteoglycans to a fibronectin substrate may affect several stages in the cell adhesion process.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Huang, G., Zhang, Y., Kim, B., Ge, G., Annis, D. S., Mosher, D. F., Greenspan, D. S. (2009). Fibronectin Binds and Enhances the Activity of Bone Morphogenetic Protein 1. J. Biol. Chem. 284: 25879-25888 [Abstract] [Full Text]
Iacob, D., Cai, J., Tsonis, M., Babwah, A., Chakraborty, C., Bhattacharjee, R. N., Lala, P. K. (2008). Decorin-Mediated Inhibition of Proliferation and Migration of the Human Trophoblast via Different Tyrosine Kinase Receptors. Endocrinology 149: 6187-6197 [Abstract] [Full Text]
Sullivan, M. M., Barker, T. H., Funk, S. E., Karchin, A., Seo, N. S., Hook, M., Sanders, J., Starcher, B., Wight, T. N., Puolakkainen, P., Sage, E. H. (2006). Matricellular Hevin Regulates Decorin Production and Collagen Assembly. J. Biol. Chem. 281: 27621-27632 [Abstract] [Full Text]
Zanotti, S., Negri, T., Cappelletti, C., Bernasconi, P., Canioni, E., Di Blasi, C., Pegoraro, E., Angelini, C., Ciscato, P., Prelle, A., Mantegazza, R., Morandi, L., Mora, M. (2005). Decorin and biglycan expression is differentially altered in several muscular dystrophies. Brain 128: 2546-2555 [Abstract] [Full Text]
Kozaki, T., Matsui, Y., Gu, J., Nishiuchi, R., Sugiura, N., Kimata, K., Ozono, K., Yoshikawa, H., Sekiguchi, K. (2003). Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues. J. Biol. Chem. 278: 50546-50553 [Abstract] [Full Text]
Tufvesson, E., Westergren-Thorsson, G. (2003). Biglycan and decorin induce morphological and cytoskeletal changes involving signalling by the small GTPases RhoA and Rac1 resulting in lung fibroblast migration. J. Cell Sci. 116: 4857-4864 [Abstract] [Full Text]
Schaefer, L., Beck, K.-F., Raslik, I., Walpen, S., Mihalik, D., Micegova, M., Macakova, K., Schonherr, E., Seidler, D. G., Varga, G., Schaefer, R. M., Kresse, H., Pfeilschifter, J. (2003). Biglycan, a Nitric Oxide-regulated Gene, Affects Adhesion, Growth, and Survival of Mesangial Cells. J. Biol. Chem. 278: 26227-26237 [Abstract] [Full Text]
Takagi, H., Asano, Y., Yamakawa, N., Matsumoto, I., Kimata, K. (2002). Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains. J. Cell Sci. 115: 3309-3318 [Abstract] [Full Text]
Xu, G., Guimond, M.-J., Chakraborty, C., Lala, P. K. (2002). Control of Proliferation, Migration, and Invasiveness of Human Extravillous Trophoblast by Decorin, a Decidual Product. Biol. Reprod. 67: 681-689 [Abstract] [Full Text]
Kinsella, M. G., Fischer, J. W., Mason, D. P., Wight, T. N. (2000). Retrovirally Mediated Expression of Decorin by Macrovascular Endothelial Cells. EFFECTS ON CELLULAR MIGRATION AND FIBRONECTIN FIBRILLOGENESIS IN VITRO. J. Biol. Chem. 275: 13924-13932 [Abstract] [Full Text]
Ungefroren, H., Gellersen, B., Krull, N. B., Kalthoff, H. (1998). Biglycan Gene Expression in the Human Leiomyosarcoma Cell Line SK-UT-1. BASAL AND PROTEIN KINASE A-INDUCED TRANSCRIPTION INVOLVES BINDING OF Sp1-LIKE/Sp3 PROTEINS IN THE PROXIMAL PROMOTER REGION. J. Biol. Chem. 273: 29230-29240 [Abstract] [Full Text]
Oksala, O., Haapasalmi, K., Hakkinen, L., Uitto, V.-J., Larjava, H. (1997). Expression of Heparan Sulphate and Small Dermatan/Chondroitin Sulphate Proteoglycans in Chronically Inflamed Human Periodontium. JDR 76: 1250-1259 [Abstract]
Nelimarkka, L., Kainulainen, V., Schonherr, E., Moisander, S., Jortikka, M., Lammi, M., Elenius, K., Jalkanen, M., Jarvelainen, H. (1997). Expression of Small Extracellular Chondroitin/Dermatan Sulfate Proteoglycans Is Differentially Regulated in Human Endothelial Cells. J. Biol. Chem. 272: 12730-12737 [Abstract] [Full Text]
Kinsella, M. G., Tsoi, C. K., Jarvelainen, H. T., Wight, T. N. (1997). Selective Expression and Processing of Biglycan during Migration of Bovine Aortic Endothelial Cells. THE ROLE OF ENDOGENOUS BASIC FIBROBLAST GROWTH FACTOR. J. Biol. Chem. 272: 318-325 [Abstract] [Full Text]
Hocking, A. M., Strugnell, R. A., Ramamurthy, P., McQuillan, D. J. (1996). Eukaryotic Expression of Recombinant Biglycan. POST-TRANSLATIONAL PROCESSING AND THE IMPORTANCE OF SECONDARY STRUCTURE FOR BIOLOGICAL ACTIVITY. J. Biol. Chem. 271: 19571-19577 [Abstract] [Full Text]
HUNZIKER, E. B., ROSENBERG, L. C. (1996). Repair of Partial-Thickness Defects in Articular Cartilage: Cell Recruitment from the Synovial Membrane. JBJS 78: 721-33 [Abstract] [Full Text]
Yamagata, M, Kimata, K (1994). Repression of a malignant cell-substratum adhesion phenotype by inhibiting the production of the anti-adhesive proteoglycan, PG-M/versican. J. Cell Sci. 107: 2581-2590 [Abstract]
Fernaud-Espinosa, I, Nieto-Sampedro, M, Bovolenta, P (1994). Differential effects of glycosaminoglycans on neurite outgrowth from hippocampal and thalamic neurones. J. Cell Sci. 107: 1437-1448 [Abstract]