Tissue distribution and subcellular localization of mammalian myosin I

doi:10.1083/jcb.119.1.163

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Tissue distribution and subcellular localization of mammalian myosin I

MC Wagner, B Barylko and JP Albanesi
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.

Myosin I, a nonfilamentous single-headed actin-activated ATPase, has recently been purified from mammalian tissue (Barylko, B., M. C. Wagner, O. Reizes, and J. P. Albanesi. 1992. Proc. Natl. Acad. Sci. USA. 89:490-494). To investigate the distribution of this enzyme in cells and tissues mAbs were generated against myosin I purified from bovine adrenal gland. Eight antibodies were characterized, five of them (M4-M8) recognize epitope(s) on the catalytic "head" portion of myosin I while the other three (M1-M3) react with the "tail" domain. Immunoblot analysis using antiadrenal myosin I antibody M2 demonstrates the widespread distribution of the enzyme in mammalian tissues. Myosin I was immunolocalized in several cell types including bovine kidney (MDBK), rat kidney (NRK), rat brain, rat phaeochromocytoma (PC12), fibroblast (Swiss 3T3), and CHO cells. In all cases, myosin I was concentrated at the cell periphery. The most intense labeling was observed in regions of the cell usually associated with motile activity (i.e., filopodia, lamellipodia and growth cones). These results are consistent with earlier observations on protozoan myosin I that suggest a motile role for the enzyme at the plasma membrane.
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Buss, F., Kendrick-Jones, J., Lionne, C., Knight, A. E., Cote, G. P., Paul Luzio, J. (1998). The Localization of Myosin VI at the Golgi Complex and Leading Edge of Fibroblasts and Its Phosphorylation and Recruitment into Membrane Ruffles of A431 Cells after Growth Factor Stimulation. JCB 143: 1535-1545 [Abstract] [Full Text]
Wylie, S. R., Wu, P.-j., Patel, H., Chantler, P. D. (1998). A conventional myosin motor drives neurite outgrowth. Proc. Natl. Acad. Sci. USA 95: 12967-12972 [Abstract] [Full Text]
Zhu, T., Beckingham, K., Ikebe, M. (1998). High Affinity Ca2+ Binding Sites of Calmodulin Are Critical for the Regulation of Myosin Ibeta Motor Function. J. Biol. Chem. 273: 20481-20486 [Abstract] [Full Text]
Steyger, P. S., Gillespie, P. G., Baird, R. A. (1998). Myosin Ibeta Is Located at Tip Link Anchors in Vestibular Hair Bundles. J. Neurosci. 18: 4603-4615 [Abstract] [Full Text]
Lin, H. C., Barylko, B., Achiriloaie, M., Albanesi, J. P. (1997). Phosphatidylinositol (4,5)-Bisphosphate-dependent Activation of Dynamins I and II Lacking the Proline/Arginine-rich Domains. J. Biol. Chem. 272: 25999-26004 [Abstract] [Full Text]
Nowak, G., Pestic-Dragovich, L., Hozak, P., Philimonenko, A., Simerly, C., Schatten, G., de Lanerolle, P. (1997). Evidence for the Presence of Myosin I in the Nucleus. J. Biol. Chem. 272: 17176-17181 [Abstract] [Full Text]
Prekeris, R., Terrian, D. M. (1997). Brain Myosin V Is a Synaptic Vesicle-associated Motor Protein: Evidence for a Ca2+-dependent Interaction with the Synaptobrevin-Synaptophysin Complex. JCB 137: 1589-1601 [Abstract] [Full Text]
Hasson, T., Gillespie, P. G., Garcia, J. A., MacDonald, R. B., Zhao, Y.-d., Yee, A. G., Mooseker, M. S., Corey, D. P. (1997). Unconventional Myosins in Inner-Ear Sensory Epithelia. JCB 137: 1287-1307 [Abstract] [Full Text]
Novak, K. D., Titus, M. A. (1997). Myosin I Overexpression Impairs Cell Migration. JCB 136: 633-647 [Abstract] [Full Text]
Hayashi, K., Ishikawa, R., Ye, L.-H., He, X.-L., Takata, K., Kohama, K., Shirao, T. (1996). Modulatory Role of Drebrin on the Cytoskeleton within Dendritic Spines in the Rat Cerebral Cortex. J. Neurosci. 16: 7161-7170 [Abstract] [Full Text]
Lee, S.-F., Egelhoff, T. T., Mahasneh, A., Cote, G. P. (1996). Cloning and Characterization of a Dictyostelium Myosin I Heavy Chain Kinase Activated by Cdc42 and Rac. J. Biol. Chem. 271: 27044-27048 [Abstract] [Full Text]
Hasson, T., Mooseker, M. S. (1996). Vertebrate Unconventional Myosins. J. Biol. Chem. 271: 16431-16434 [Full Text]
Temesvari, L. A., Bush, J. M., Peterson, M. D., Novak, K. D., Titus, M. A., Cardelli, J. A. (1996). Examination of the endosomal and lysosomal pathways in Dictyostelium discoideum myosin I mutants. J. Cell Sci. 109: 663-673 [Abstract]
Pelham, R., Lin, J., Wang, Y. (1996). A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport. J. Cell Sci. 109: 981-989 [Abstract]
Ruppert, C, Godel, J, Muller, R., Kroschewski, R, Reinhard, J, Bahler, M (1995). Localization of the rat myosin I molecules myr 1 and myr 2 and in vivo targeting of their tail domains. J. Cell Sci. 108: 3775-3786 [Abstract]
Peterson, M., Novak, K., Reedy, M., Ruman, J., Titus, M. (1995). Molecular genetic analysis of myoC, a Dictyostelium myosin I. J. Cell Sci. 108: 1093-1103 [Abstract]
Had, L, Faivre-Sarrailh, C, Legrand, C, Mery, J, Brugidou, J, Rabie, A (1994). Tropomyosin isoforms in rat neurons: the different developmental profiles and distributions of TM-4 and TMBr-3 are consistent with different functions. J. Cell Sci. 107: 2961-2973 [Abstract]
D'Andrea, L, Danon, M., Sgourdas, G., Bonder, E. (1994). Identification of coelomocyte unconventional myosin and its association with in vivo particle/vesicle motility. J. Cell Sci. 107: 2081-2094 [Abstract]
Coluccio, L. (1994). Differential calmodulin binding to three myosin-1 isoforms from liver. J. Cell Sci. 107: 2279-2284 [Abstract]
Langford, G., Kuznetsov, S., Johnson, D, Cohen, D., Weiss, D. (1994). Movement of axoplasmic organelles on actin filaments assembled on acrosomal processes: evidence for a barbed-end-directed organelle motor. J. Cell Sci. 107: 2291-2298 [Abstract]
Footer, M, Bretscher, A (1994). Brush border myosin-I microinjected into cultured cells is targeted to actin-containing surface structures. J. Cell Sci. 107: 1623-1631 [Abstract]
Luna, E., Hitt, A. (1992). Cytoskeleton--plasma membrane interactions. Science 258: 955-964 [Abstract]
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Biemesderfer, D., Mentone, S. A., Mooseker, M., Hasson, T. (2002). Expression of myosin VI within the early endocytic pathway in adult and developing proximal tubules. Am. J. Physiol. Renal Physiol. 282: F785-F794 [Abstract] [Full Text]