The Journal of Cell Biology, Vol 119, 17-25, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract

N Ulitzur, A Harel, N Feinstein and Y Gruenbaum
Department of Genetics, Hebrew University of Jerusalem, Israel.

The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a Drosophila in vitro assembly system that reconstitutes nuclei from added sperm chromatin or naked DNA. Upon incubation of the embryonic assembly extract with anti-Drosophila lamin antibodies, the attachment of nuclear membrane vesicles to chromatin surface and nuclear envelope formation did not occur. Lamina assembly and nuclear membrane vesicles attachment to the chromatin were inhibited only when the activity of the 75-kD lamin isoform was inhibited in both soluble and membrane-vesicles fractions. Incubation of decondensed sperm chromatin with an extract that was depleted of nuclear membranes revealed the presence of lamin molecules on the chromatin periphery. In addition, high concentrations of bacterially expressed lamin molecules added to the extract, were able to associate with the chromatin periphery, and did not inhibit nuclear envelope assembly. After nuclear reconstitution, a fraction of the lamin pool was converted into the typical 74- and 76-kD isoforms. Together, these data strongly support an essential role of the lamina in nuclear envelope assembly.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Basso, A. D., Kirschmeier, P., Bishop, W. R. (2006). Thematic review series: Lipid Posttranslational Modifications. Farnesyl transferase inhibitors. J. Lipid Res. 47: 15-31 [Abstract] [Full Text]  
• Goldman, R. D., Gruenbaum, Y., Moir, R. D., Shumaker, D. K., Spann, T. P. (2002). Nuclear lamins: building blocks of nuclear architecture. Genes Dev. 16: 533-547 [Full Text]  
• Fairley, E. A. L., Riddell, A., Ellis, J. A., Kendrick-Jones, J. (2002). The cell cycle dependent mislocalisation of emerin may contribute to the Emery-Dreifuss muscular dystrophy phenotype. J. Cell Sci. 115: 341-354 [Abstract] [Full Text]  
• Lopez-Soler, R. I., Moir, R. D., Spann, T. P., Stick, R., Goldman, R. D. (2001). A role for nuclear lamins in nuclear envelope assembly. JCB 154: 61-70 [Abstract] [Full Text]  
• Lu, J, Oliver, B (2001). Drosophila OVO regulates ovarian tumor transcription by binding unusually near the transcription start site. Development 128: 1671-1686 [Abstract]  
• Moir, R. D., Yoon, M., Khuon, S., Goldman, R. D. (2000). Nuclear Lamins a and B1: Different Pathways of Assembly during Nuclear Envelope Formation in Living Cells. JCB 151: 1155-1168 [Abstract] [Full Text]  
• Lourim, D, Krohne, G (1999). Chromatin binding and polymerization of the endogenous Xenopus egg lamins: the opposing effects of glycogen and ATP. J. Cell Sci. 111: 3675-3686 [Abstract]  
• Goldberg, M., Harel, A., Brandeis, M., Rechsteiner, T., Richmond, T. J., Weiss, A. M., Gruenbaum, Y. (1999). The tail domain of lamin Dm0 binds histones H2A and H2B. Proc. Natl. Acad. Sci. USA 96: 2852-2857 [Abstract] [Full Text]  
• Drummond, S., Ferrigno, P., Lyon, C., Murphy, J., Goldberg, M., Allen, T., Smythe, C., Hutchison, C. J. (1999). Temporal Differences in the Appearance of NEP-B78 and an LBR-like Protein during Xenopus Nuclear Envelope Reassembly Reflect the Ordered Recruitment of Functionally Discrete Vesicle Types. JCB 144: 225-240 [Abstract] [Full Text]  
• Yang, L., Guan, T., Gerace, L. (1997). Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis. JCB 137: 1199-1210 [Abstract] [Full Text]  
• Lenz-Bohme, B., Wismar, J., Fuchs, S., Reifegerste, R., Buchner, E., Betz, H., Schmitt, B. (1997). Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae. JCB 137: 1001-1016 [Abstract] [Full Text]  
• Klapper, M, Exner, K, Kempf, A, Gehrig, C, Stuurman, N, Fisher, P., Krohne, G (1997). Assembly of A- and B-type lamins studied in vivo with the baculovirus system. J. Cell Sci. 110: 2519-2532 [Abstract]  
• Lopez, J., Wolfner, M. (1997). The developmentally regulated Drosophila embryonic nuclear lamina protein 'Young Arrest' (fs(1)Ya) is capable of associating with chromatin. J. Cell Sci. 110: 643-651 [Abstract]  
• Paddy, M. R., Saumweber, H., Agard, D. A., Sedat, J. W. (1996). Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos. J. Cell Sci. 109: 591-607 [Abstract]  
• Lourim, D, Kempf, A, Krohne, G (1996). Characterization and quantitation of three B-type lamins in Xenopus oocytes and eggs: increase of lamin LI protein synthesis during meiotic maturation. J. Cell Sci. 109: 1775-1785 [Abstract]  
• Goldberg, M, Jenkins, H, Allen, T, Whitfield, W., Hutchison, C. (1995). Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts. J. Cell Sci. 108: 3451-3461 [Abstract]  
• Stuurman, N, Maus, N, Fisher, P. (1995). Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J. Cell Sci. 108: 3137-3144 [Abstract]  
• Hutchison, C., Bridger, J., Cox, L., Kill, I. (1994). Weaving a pattern from disparate threads: lamin function in nuclear assembly and DNA replication. J. Cell Sci. 107: 3259-3269 [Abstract]  
• Hennekes, H, Nigg, E. (1994). The role of isoprenylation in membrane attachment of nuclear lamins. A single point mutation prevents proteolytic cleavage of the lamin A precursor and confers membrane binding properties. J. Cell Sci. 107: 1019-1029 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents