Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion [published erratum appears in J Cell Biol 1993 Feb;120(4):1071]

doi:10.1083/jcb.119.1.215

This Article

	Full Text (PDF, 4271K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Erbe, D. V.
	Articles by Brandley, B. K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Erbe, D. V.
	Articles by Brandley, B. K.


Social Bookmarking

	What's this?

ARTICLES

Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion [published erratum appears in J Cell Biol 1993 Feb;120(4):1071]

DV Erbe, BA Wolitzky, LG Presta, CR Norton, RJ Ramos, DK Burns, JM Rumberger, BN Rao, C Foxall and BK Brandley
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.

E-selectin elicits cell adhesion by binding to the cell surface carbohydrate, sialyl Lewis X (sLe(x)). We evaluated the effects of mutations in the E-selectin lectin domain on the binding of a panel of anti-E-selectin mAbs and on the recognition of immobilized sLe(x) glycolipid. Functional residues were then superimposed onto a three- dimensional model of the E-selectin lectin domain. This analysis demonstrated that the epitopes recognized by blocking mAbs map to a patch near the antiparallel beta sheet derived from the NH2 and COOH termini of the lectin domain and two adjacent loops. Mutations that affect sLe(x) binding map to this same region. These results thus define a small region of the E-selectin lectin domain that is critical for carbohydrate recognition.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Huang, J., Chen, J., Chesla, S. E., Yago, T., Mehta, P., McEver, R. P., Zhu, C., Long, M. (2004). Quantifying the Effects of Molecular Orientation and Length on Two-dimensional Receptor-Ligand Binding Kinetics. J. Biol. Chem. 279: 44915-44923 [Abstract] [Full Text]
Cardones, A. R., Murakami, T., Hwang, S. T. (2003). CXCR4 Enhances Adhesion of B16 Tumor Cells to Endothelial Cells in Vitro and in Vivo via {beta}1 Integrin. Cancer Res. 63: 6751-6757 [Abstract] [Full Text]
Bouyain, S., Rushton, S., Drickamer, K. (2001). Minimal requirements for the binding of selectin ligands to a C-type carbohydrate-recognition domain. Glycobiology 11: 989-996 [Abstract] [Full Text]
Fitzhugh, D. J., Naik, S., Caughman, S. W., Hwang, S. T. (2000). Cutting Edge: C-C Chemokine Receptor 6 Is Essential for Arrest of a Subset of Memory T Cells on Activated Dermal Microvascular Endothelial Cells Under Physiologic Flow Conditions In Vitro. J. Immunol. 165: 6677-6681 [Abstract] [Full Text]
Reidling, J. C., Miller, M. A., Steele, R. E. (2000). Sweet Tooth, a Novel Receptor Protein-tyrosine Kinase with C-type Lectin-like Extracellular Domains. J. Biol. Chem. 275: 10323-10330 [Abstract] [Full Text]
De Caterina, R., Liao, J. K, Libby, P. (2000). Fatty acid modulation of endothelial activation. Am. J. Clin. Nutr. 71: 213S-223S [Abstract] [Full Text]
Torgersen, D., Mullin, N. P., Drickamer, K. (1998). Mechanism of Ligand Binding to E- and P-selectin Analyzed Using Selectin/Mannose-binding Protein Chimeras. J. Biol. Chem. 273: 6254-6261 [Abstract] [Full Text]
Bajorath, J., Greenfield, B., Munro, S. B., Day, A. J., Aruffo, A. (1998). Identification of CD44 Residues Important for Hyaluronan Binding and Delineation of the Binding Site. J. Biol. Chem. 273: 338-343 [Abstract] [Full Text]
Mullin, N. P., Hitchen, P. G., Taylor, M. E. (1997). Mechanism of Ca2+ and Monosaccharide Binding to a C-type Carbohydrate-recognition Domain of the Macrophage Mannose Receptor. J. Biol. Chem. 272: 5668-5681 [Abstract] [Full Text]
Ridings, P. C., Holloway, S., Bloomfield, G. L., Phillips, M. L., Fisher, B. J., Blocher, C. R., Sugerman, H. J., Fowler, A. A. III (1997). Protective role of synthetic sialylated oligosaccharide in sepsis-induced acute lung injury. J. Appl. Physiol. 82: 644-651 [Abstract] [Full Text]
Wu, K., Yuan, J., Lasky, L. A. (1996). Characterization of a Novel Member of the Macrophage Mannose Receptor Type C Lectin Family. J. Biol. Chem. 271: 21323-21330 [Abstract] [Full Text]
Revelle, B. M., Scott, D., Beck, P. J. (1996). Single Amino Acid Residues in the E- and P-selectin Epidermal Growth Factor Domains Can Determine Carbohydrate Binding Specificity. J. Biol. Chem. 271: 16160-16170 [Abstract] [Full Text]
Vinson, M., van der Merwe, P. A., Kelm, S�r., May, A., Jones, E. Y., Crocker, P. R. (1996). Characterization of the Sialic Acid-binding Site in Sialoadhesin by Site-directed Mutagenesis. J. Biol. Chem. 271: 9267-9272 [Abstract] [Full Text]
Blanck, O., Iobst, S. T., Gabel, C., Drickamer, K. (1996). Introduction of Selectin-like Binding Specificity into a Homologous Mannose-binding Protein. J. Biol. Chem. 271: 7289-7292 [Abstract] [Full Text]
Puri, K. D., Springer, T. A. (1996). A Schiff Base with Mildly Oxidized Carbohydrate Ligands Stabilizes L-selectin and not P-selectin or E-selectin Rolling Adhesions in Shear Flow. J. Biol. Chem. 271: 5404-5413 [Abstract] [Full Text]
Revelle, B. M., Scott, D., Kogan, T. P., Zheng, J., Beck, P. J. (1996). Structure-Function Analysis of P-selectin-Sialyl Lewis^X Binding Interactions. J. Biol. Chem. 271: 4289-4297 [Abstract] [Full Text]
Ng, K. K.-S., Drickamer, K., Weis, W. I. (1996). Structural Analysis of Monosaccharide Recognition by Rat Liver Mannose-binding Protein. J. Biol. Chem. 271: 663-674 [Abstract] [Full Text]
Kogan, T. P., Revelle, B. M., Tapp, S., Scott, D., Beck, P. J. (1995). A Single Amino Acid Residue Can Determine the Ligand Specificity of E-selectin. J. Biol. Chem. 270: 14047-14055 [Abstract] [Full Text]
Anostario, M. Jr., Huang, K.-S. (1995). Modulation of E-selectin Structure/Function by Metal Ions. J. Biol. Chem. 270: 8138-8144 [Abstract] [Full Text]