Effects of brefeldin A on endocytosis, transcytosis and transport to the Golgi complex in polarized MDCK cells

doi:10.1083/jcb.119.2.259

This Article

	Full Text (PDF, 6283K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Prydz, K.
	Articles by van Deurs, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Prydz, K.
	Articles by van Deurs, B.


Social Bookmarking

	What's this?

ARTICLES

Effects of brefeldin A on endocytosis, transcytosis and transport to the Golgi complex in polarized MDCK cells

K Prydz, SH Hansen, K Sandvig and B van Deurs
Institute for Cancer Research, Norwegian Radium Hospital, Oslo.

We have studied the effects of brefeldin A (BFA) on endocytosis and intracellular traffic in polarized MDCK cells by using the galactose- binding protein toxin ricin as a membrane marker and HRP as a marker of fluid phase transport. We found that BFA treatment rapidly increased apical endocytosis of both ricin and HRP, whereas basolateral endocytosis was unaffected, as was endocytosis of HRP in the poorly polarized carcinoma cell lines HEp-2 and T47D. Tubular endosomes were induced by BFA both apically and basolaterally in some MDCK cells, comparable with those seen in HEp-2 and T47D cells. In addition, in MDCK cells, BFA induced formation of small (< 300 nm) vesicles, labeled both after apical and basolateral uptake of HRP, as well as some very large (> 700 nm) vacuoles, which were only labeled when HRP was present in the apical medium. In contrast, neither in MDCK nor in HEp-2 or T47D cells, did BFA have any effect on lysosomal morphology. Moreover, transcytosis in the basolateral-apical direction was stimulated both for HRP and ricin. Other vesicular transport routes were less affected or unaffected by BFA treatment. Two closely related structural analogues of BFA (B16 and B21), unable to produce the changes in Golgi and endosomal morphology seen after BFA treatment in a number of different cell lines, were also unable to mimic the effects of BFA on MDCK cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Butterworth, M. B., Edinger, R. S., Frizzell, R. A., Johnson, J. P. (2009). Regulation of the epithelial sodium channel by membrane trafficking. Am. J. Physiol. Renal Physiol. 296: F10-F24 [Abstract] [Full Text]
Ferrari, A., Veligodskiy, A., Berge, U., Lucas, M. S., Kroschewski, R. (2008). ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation. J. Cell Sci. 121: 3649-3663 [Abstract] [Full Text]
Wallrabe, H., Bonamy, G., Periasamy, A., Barroso, M. (2007). Receptor Complexes Cotransported via Polarized Endocytic Pathways Form Clusters with Distinct Organizations. Mol. Biol. Cell 18: 2226-2243 [Abstract] [Full Text]
Wang, Q., Kong, L., Hao, H., Wang, X., Lin, J., Samaj, J., Baluska, F. (2005). Effects of Brefeldin A on Pollen Germination and Tube Growth. Antagonistic Effects on Endocytosis and Secretion. Plant Physiol. 139: 1692-1703 [Abstract] [Full Text]
Rilla, K., Siiskonen, H., Spicer, A. P., Hyttinen, J. M. T., Tammi, M. I., Tammi, R. H. (2005). Plasma Membrane Residence of Hyaluronan Synthase Is Coupled to Its Enzymatic Activity. J. Biol. Chem. 280: 31890-31897 [Abstract] [Full Text]
Butterworth, M. B., Edinger, R. S., Johnson, J. P., Frizzell, R. A. (2004). Acute ENaC Stimulation by cAMP in a Kidney Cell Line is Mediated by Exocytic Insertion from a Recycling Channel Pool. JGP 125: 81-101 [Abstract] [Full Text]
Sarnataro, D., Campana, V., Paladino, S., Stornaiuolo, M., Nitsch, L., Zurzolo, C. (2004). PrPC Association with Lipid Rafts in the Early Secretory Pathway Stabilizes Its Cellular Conformation. Mol. Biol. Cell 15: 4031-4042 [Abstract] [Full Text]
Wakabayashi, Y., Lippincott-Schwartz, J., Arias, I. M. (2004). Intracellular Trafficking of Bile Salt Export Pump (ABCB11) in Polarized Hepatic Cells: Constitutive Cycling between the Canalicular Membrane and rab11-positive Endosomes. Mol. Biol. Cell 15: 3485-3496 [Abstract] [Full Text]
Fjeldstad, K., Pedersen, M. E., Vuong, T. T., Kolset, S. O., Nordstrand, L. M., Prydz, K. (2002). Sulfation in the Golgi Lumen of Madin-Darby Canine Kidney Cells Is Inhibited by Brefeldin A and Depends on a Factor Present in the Cytoplasm and on Golgi Membranes. J. Biol. Chem. 277: 36272-36279 [Abstract] [Full Text]
Wang, E., Pennington, J. G., Goldenring, J. R., Hunziker, W., Dunn, K. W. (2002). Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells. J. Cell Sci. 114: 3309-3321 [Abstract] [Full Text]
Huang, S.-N., Swaan, P. W. (2000). Involvement of a Receptor-Mediated Component in Cellular Translocation of Riboflavin. J. Pharmacol. Exp. Ther. 294: 117-125 [Abstract] [Full Text]
Turner, M. D., Arvan, P. (2000). Protein Traffic from the Secretory Pathway to the Endosomal System in Pancreatic beta -Cells. J. Biol. Chem. 275: 14025-14030 [Abstract] [Full Text]
Chen, C.-S., Bach, G., Pagano, R. E. (1998). Abnormal transport along the lysosomal pathway in Mucolipidosis, type IV disease. Proc. Natl. Acad. Sci. USA 95: 6373-6378 [Abstract] [Full Text]
Llorente, A., Rapak, A., Schmid, S. L., van Deurs, B., Sandvig, K. (1998). Expression of Mutant Dynamin Inhibits Toxicity and Transport of Endocytosed Ricin to the Golgi Apparatus. JCB 140: 553-563 [Abstract] [Full Text]
Jareb, M., Banker, G. (1997). Inhibition of Axonal Growth by Brefeldin A in Hippocampal Neurons in Culture. J. Neurosci. 17: 8955-8963 [Abstract] [Full Text]
Lin, S., Naim, H. Y., Roth, M. G. (1997). Tyrosine-dependent Basolateral Sorting Signals Are Distinct from Tyrosine-dependent Internalization Signals. J. Biol. Chem. 272: 26300-26305 [Abstract] [Full Text]
Saunders, C., Limbird, L. E. (1997). Disruption of Microtubules Reveals Two Independent Apical Targeting Mechanisms for G-protein-coupled Receptors in Polarized Renal Epithelial Cells. J. Biol. Chem. 272: 19035-19045 [Abstract] [Full Text]
Beau, I., Misrahi, M., Gross, B., Vannier, B., Loosfelt, H., Hai, M. T. V., Pichon, C., Milgrom, E. (1997). Basolateral Localization and Transcytosis of Gonadotropin and Thyrotropin Receptors Expressed in Madin-Darby Canine Kidney Cells. J. Biol. Chem. 272: 5241-5248 [Abstract] [Full Text]
Shurety, W, Bright, N., Luzio, J. (1996). The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco-2 cells. J. Cell Sci. 109: 2927-2935 [Abstract]
Ralston, E, Ploug, T (1996). GLUT4 in cultured skeletal myotubes is segregated from the transferrin receptor and stored in vesicles associated with TGN. J. Cell Sci. 109: 2967-2978 [Abstract]
Reich, V, Mostov, K, Aroeti, B (1996). The basolateral sorting signal of the polymeric immunoglobulin receptor contains two functional domains. J. Cell Sci. 109: 2133-2139 [Abstract]
Hansen, S. H., Olsson, A., Casanova, J. E. (1995). Wortmannin, an Inhibitor of Phosphoinositide 3-Kinase, Inhibits Transcytosis in Polarized Epithelial Cells. J. Biol. Chem. 270: 28425-28432 [Abstract] [Full Text]
Arreaza, G., Brown, D. A. (1995). Sorting and Intracellular Trafficking of a Glycosylphosphatidylinositol-anchored Protein and Two Hybrid Transmembrane Proteins with the Same Ectodomain in Madin-Darby Canine Kidney Epithelial Cells. J. Biol. Chem. 270: 23641-23647 [Abstract] [Full Text]
Jackman, M., Shurety, W, Ellis, J., Luzio, J. (1994). Inhibition of apical but not basolateral endocytosis of ricin and folate in Caco-2 cells by cytochalasin D. J. Cell Sci. 107: 2547-2556 [Abstract]