Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA

doi:10.1083/jcb.119.2.427

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Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA

JL Rodriguez Fernandez, B Geiger, D Salomon, I Sabanay, M Zoller and A Ben-Ze'ev
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.

Transfection of chicken vinculin cDNA into two tumor cell lines expressing diminished levels of the endogenous protein, brought about a drastic suppression of their tumorigenic ability. The SV-40-transformed Balb/c 3T3 line (SVT2) contains four times less vinculin than the parental 3T3 cells, and the rat adenocarcinoma BSp73ASML has no detectable vinculin. Restoration of vinculin in these cells, up to the levels found in 3T3 cells, resulted in an apparent increase in substrate adhesiveness, a decrease in the ability to grow in soft agar, and suppression of their capacity to develop tumors after injection into syngeneic hosts or nude mice. These results suggest that vinculin, a cytoplasmic component of cell-matrix and cell-cell adhesions, may have a major suppressive effect on the transformed phenotype.
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Le Clainche, C., Carlier, M.-F. (2008). Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration. Physiol. Rev. 88: 489-513 [Abstract] [Full Text]
Chen, H., Choudhury, D. M., Craig, S. W. (2006). Coincidence of Actin Filaments and Talin Is Required to Activate Vinculin. J. Biol. Chem. 281: 40389-40398 [Abstract] [Full Text]
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Lu, Q.-Y., Jin, Y.-S., Pantuck, A., Zhang, Z.-F., Heber, D., Belldegrun, A., Brooks, M., Figlin, R., Rao, J. (2005). Green Tea Extract Modulates Actin Remodeling via Rho Activity in an In vitro Multistep Carcinogenic Model. Clin. Cancer Res. 11: 1675-1683 [Abstract] [Full Text]
Subauste, M. C., Pertz, O., Adamson, E. D., Turner, C. E., Junger, S., Hahn, K. M. (2004). Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. JCB 165: 371-381 [Abstract] [Full Text]
Wittenmayer, N., Jandrig, B., Rothkegel, M., Schluter, K., Arnold, W., Haensch, W., Scherneck, S., Jockusch, B. M. (2004). Tumor Suppressor Activity of Profilin Requires a Functional Actin Binding Site. Mol. Biol. Cell 15: 1600-1608 [Abstract] [Full Text]
Whitfield, M. L., Sherlock, G., Saldanha, A. J., Murray, J. I., Ball, C. A., Alexander, K. E., Matese, J. C., Perou, C. M., Hurt, M. M., Brown, P. O., Botstein, D. (2002). Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in Tumors. Mol. Biol. Cell 13: 1977-2000 [Abstract] [Full Text]
Suwa, A., Mitsushima, M., Ito, T., Akamatsu, M., Ueda, K., Amachi, T., Kioka, N. (2002). Vinexin beta Regulates the Anchorage Dependence of ERK2 Activation Stimulated by Epidermal Growth Factor. J. Biol. Chem. 277: 13053-13058 [Abstract] [Full Text]
Nikolopoulos, S. N., Turner, C. E. (2000). Actopaxin, a New Focal Adhesion Protein That Binds Paxillin Ld Motifs and Actin and Regulates Cell Adhesion. JCB 151: 1435-1448 [Abstract] [Full Text]
Koh, T. J., Tidball, J. G. (2000). Nitric oxide inhibits calpain-mediated proteolysis of talin in skeletal muscle cells. Am. J. Physiol. Cell Physiol. 279: C806-C812 [Abstract] [Full Text]
Janke, J., Schluter, K., Jandrig, B., Theile, M., Kolble, K., Arnold, W., Grinstein, E., Schwartz, A., Estevez-Schwarz, L., Schlag, P. M., Jockusch, B. M., Scherneck, S. (2000). Suppression of Tumorigenicity in Breast Cancer Cells by the Microfilament Protein Profilin 1. JEM 191: 1675-1686 [Abstract] [Full Text]
FREY, B. M., REBER, B. F. X., VISHWANATH, B. S., ESCHER, G., FREY, F. J. (1999). Annexin I modulates cell functions by controlling intracellular calcium release. FASEB J. 13: 2235-2245 [Abstract] [Full Text]
Steimle, P. A., Hoffert, J. D., Adey, N. B., Craig, S. W. (1999). Polyphosphoinositides Inhibit the Interaction of Vinculin with Actin Filaments. J. Biol. Chem. 274: 18414-18420 [Abstract] [Full Text]
Rodríguez-Fernández, J. L., Gómez, M., Luque, A., Hogg, N., Sánchez-Madrid, F., Cabañas, C. (1999). The Interaction of Activated Integrin Lymphocyte Function-associated Antigen 1�with Ligand Intercellular Adhesion Molecule 1�Induces Activation and Redistribution of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2�in T Lymphocytes. Mol. Biol. Cell 10: 1891-1907 [Abstract] [Full Text]
Liu, K., Li, L., Nisson, P. E., Gruber, C., Jessee, J., Cohen, S. N. (1999). Reversible Tumorigenesis Induced by Deficiency of Vasodilator-Stimulated Phosphoprotein. Mol. Cell. Biol. 19: 3696-3703 [Abstract] [Full Text]
Yang, W., Lin, Q., Guan, J.-L., Cerione, R. A. (1999). Activation of the Cdc42-associated Tyrosine Kinase-2 (ACK-2) by Cell Adhesion via Integrin beta 1. J. Biol. Chem. 274: 8524-8530 [Abstract] [Full Text]
He, H., Watanabe, T., Zhan, X., Huang, C., Schuuring, E., Fukami, K., Takenawa, T., Kumar, C. C., Simpson, R. J., Maruta, H. (1998). Role of Phosphatidylinositol 4,5-Bisphosphate in Ras/Rac-Induced Disruption of the Cortactin-Actomyosin II Complex and Malignant Transformation. Mol. Cell. Biol. 18: 3829-3837 [Abstract] [Full Text]
Simcha, I., Shtutman, M., Salomon, D., Zhurinsky, J., Sadot, E., Geiger, B., Ben-Ze'ev, A. (1998). Differential Nuclear Translocation and Transactivation Potential of {beta}-Catenin and Plakoglobin. JCB 141: 1433-1448 [Abstract] [Full Text]
Aplin, A. E., Howe, A., Alahari, S. K., Juliano, R. L. (1998). Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins. Pharmacol. Rev. 50: 197-264 [Abstract] [Full Text]
Perez-Moreno, M, Avila, A, Islas, S, Sanchez, S, Gonzalez-Mariscal, L (1998). Vinculin but not alpha-actinin is a target of PKC phosphorylation during junctional assembly induced by calcium. J. Cell Sci. 111: 3563-3571 [Abstract]
Salomon, D., Sacco, P. A., Roy, S. G., Simcha, I., Johnson, K. R., Wheelock, M. J., Ben-Ze'ev, A. (1997). Regulation of {beta}-Catenin Levels and Localization by Overexpression of Plakoglobin and Inhibition of the Ubiquitin-Proteasome System. JCB 139: 1325-1335 [Abstract] [Full Text]
Kang, J.-S., Gao, M., Feinleib, J. L., Cotter, P. D., Guadagno, S. N., Krauss, R. S. (1997). CDO: An Oncogene-, Serum-, and Anchorage-regulated Member of the Ig/Fibronectin Type III Repeat Family. JCB 138: 203-213 [Abstract] [Full Text]
Sers, C., Emmenegger, U., Husmann, K., Bucher, K., Andres, A.-C., Schafer, R. (1997). Growth-inhibitory Activity and Downregulation of the Class II Tumor-suppressor Gene H-rev107 in Tumor Cell Lines and Experimental Tumors. JCB 136: 935-944 [Abstract] [Full Text]
Pankov, R, Simcha, I, Zoller, M, Oshima, R., Ben-Ze'ev, A (1997). Contrasting effects of K8 and K18 on stabilizing K19 expression, cell motility and tumorigenicity in the BSp73 adenocarcinoma. J. Cell Sci. 110: 965-974 [Abstract]
Manfruelli, P, Arquier, N, Hanratty, W., Semeriva, M (1996). The tumor suppressor gene, lethal(2)giant larvae (1(2)g1), is required for cell shape change of epithelial cells during Drosophila development. Development 122: 2283-2294 [Abstract]
Kr�mer, A., Carstens, C.-P., Fahl, W. E. (1996). A Novel CCAAT-binding Protein Necessary for Adhesion-dependent Cyclin A Transcription at the G(1)/S Boundary Is Sequestered by a Retinoblastoma-like Protein in G(0). J. Biol. Chem. 271: 6579-6582 [Abstract] [Full Text]
Albiges-Rizo, C, Frachet, P, Block, M. (1995). Down regulation of talin alters cell adhesion and the processing of the alpha 5 beta 1 integrin. J. Cell Sci. 108: 3317-3329 [Abstract]
Volberg, T, Geiger, B, Kam, Z, Pankov, R, Simcha, I, Sabanay, H, Coll, J., Adamson, E, Ben-Ze'ev, A (1995). Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption. J. Cell Sci. 108: 2253-2260 [Abstract]
Bershadsky, A., Gluck, U, Denisenko, O., Sklyarova, T., Spector, I, Ben-Ze'ev, A (1995). The state of actin assembly regulates actin and vinculin expression by a feedback loop. J. Cell Sci. 108: 1183-1193 [Abstract]
Gluck, U., Ben-Ze'ev, A. (1994). Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells. J. Cell Sci. 107: 1773-1782 [Abstract]
DePasquale, J., Samsonoff, W., Gierthy, J. (1994). 17-beta-Estradiol induced alterations of cell-matrix and intercellular adhesions in a human mammary carcinoma cell line. J. Cell Sci. 107: 1241-1254 [Abstract]
Stossel, T. (1993). On the crawling of animal cells. Science 260: 1086-1094 [Abstract]
Luna, E., Hitt, A. (1992). Cytoskeleton--plasma membrane interactions. Science 258: 955-964 [Abstract]
Pawlak, G., Helfman, D. M. (2002). Post-transcriptional Down-regulation of ROCKI/Rho-kinase through an MEK-dependent Pathway Leads to Cytoskeleton Disruption in Ras-transformed Fibroblasts. Mol. Biol. Cell 13: 336-347 [Abstract] [Full Text]