A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques

doi:10.1083/jcb.119.4.913

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A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques

Y Takada, J Ylanne, D Mandelman, W Puzon and MH Ginsberg
Committee on Vascular Biology, Scripps Research Institute, La Jolla, CA 92037.

A point mutation in a highly conserved region of the beta 1 subunit, Asp130 to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)- dependent binding of alpha 5 beta 1 to fibronectin (FN) without disrupting gross structure or heterodimer assembly. The D130A mutation also interferes with binding to invasin, a ligand that lacks RGD sequence. In spite of the lack of detectable FN binding by alpha 5 beta 1(D130A), it was recruited to adhesion plaques formed on FN by endogenous hamster receptors. Thus, intact ligand binding function is not required for recruitment of alpha 5 beta 1 to adhesion plaques. Overexpression of beta 1(D130A) partially interfered with endogenous alpha 5 beta 1 function, thus defining a dominant negative beta 1 integrin mutation.
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Isberg, R., Barnes, P (2001). Subversion of integrins by enteropathogenic Yersinia. J. Cell Sci. 114: 21-28 [Abstract]
Fornaro, M., Steger, C. A., Bennett, A. M., Wu, J. J., Languino, L. R. (2000). Differential Role of beta 1C and beta 1A Integrin Cytoplasmic Variants in Modulating Focal Adhesion Kinase, Protein Kinase B/AKT, and Ras/Mitogen-activated Protein Kinase Pathways. Mol. Biol. Cell 11: 2235-2249 [Abstract] [Full Text]
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Puzon-McLaughlin, W., Kamata, T., Takada, Y. (2000). Multiple Discontinuous Ligand-mimetic Antibody Binding Sites Define a Ligand Binding Pocket in Integrin alpha IIbbeta 3. J. Biol. Chem. 275: 7795-7802 [Abstract] [Full Text]
Levy, L., Broad, S., Diekmann, D., Evans, R. D., Watt, F. M. (2000). beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms. Mol. Biol. Cell 11: 453-466 [Abstract] [Full Text]
Kamata, T., Liddington, R. C., Takada, Y. (1999). Interaction between Collagen and the alpha 2 I-domain of Integrin alpha 2beta 1. CRITICAL ROLE OF CONSERVED RESIDUES IN THE METAL ION-DEPENDENT ADHESION SITE (MIDAS) REGION. J. Biol. Chem. 274: 32108-32111 [Abstract] [Full Text]
Hamburger, Z. A., Brown, M. S., Isberg, R. R., Bjorkman, P. J. (1999). Crystal Structure of Invasin: A Bacterial Integrin-Binding Protein. Science 286: 291-295 [Abstract] [Full Text]
Shiokawa, S., Yoshimura, Y., Sawa, H., Nagamatsu, S., Hanashi, H., Sakai, K., Ando, M., Nakamura, Y. (1999). Functional Role of Arg-Gly-Asp (RGD)-Binding Sites on �1 Integrin in Embryo Implantation Using Mouse Blastocysts and Human Decidua. Biol. Reprod. 60: 1468-1474 [Abstract] [Full Text]
Guilherme, A., Czech, M. P. (1998). Stimulation of IRS-1-associated Phosphatidylinositol 3-Kinase and Akt/Protein Kinase B but Not Glucose Transport by beta 1-Integrin Signaling in Rat Adipocytes. J. Biol. Chem. 273: 33119-33122 [Abstract] [Full Text]
Cao, Z., Huang, K., Horwitz, A. F. (1998). Identification of a Domain on the Integrin alpha 5 Subunit Implicated in Cell Spreading and Signaling. J. Biol. Chem. 273: 31670-31679 [Abstract] [Full Text]
Sakai, T., Peyruchaud, O., Fassler, R., Mosher, D. F. (1998). Restoration of beta 1A Integrins is Required for Lysophosphatidic Acid-induced Migration of beta 1-null Mouse Fibroblastic Cells. J. Biol. Chem. 273: 19378-19382 [Abstract] [Full Text]
Zhang, X.-P., Kamata, T., Yokoyama, K., Puzon-McLaughlin, W., Takada, Y. (1998). Specific Interaction of the Recombinant Disintegrin-like Domain of MDC-15 (Metargidin, ADAM-15) with Integrin alpha vbeta 3. J. Biol. Chem. 273: 7345-7350 [Abstract] [Full Text]
Tsuchida, J, Ueki, S, Takada, Y, Saito, Y, Takagi, J (1998). The 'ligand-induced conformational change' of alpha 5 beta 1 integrin. Relocation of alpha 5 subunit to uncover the beta 1 stalk region. J. Cell Sci. 111: 1759-1766 [Abstract]
Wennerberg, K, Fassler, R, Warmegard, B, Johansson, S (1998). Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin beta1A. Requirement for threonines 788-789 in receptor activation. J. Cell Sci. 111: 1117-1126 [Abstract]
Belkin, A. M., Retta, S. F., Pletjushkina, O. Y., Balzac, F., Silengo, L., Fassler, R., Koteliansky, V. E., Burridge, K., Tarone, G. (1997). Muscle {beta}1D Integrin Reinforces the Cytoskeleton-Matrix Link: Modulation of Integrin Adhesive Function by Alternative Splicing. JCB 139: 1583-1595 [Abstract] [Full Text]
Pujades, C., Alon, R., Yauch, R. L., Masumoto, A., Burkly, L. C., Chen, C., Springer, T. A., Lobb, R. R., Hemler, M. E. (1997). Defining Extracellular Integrin alpha -Chain Sites That Affect Cell Adhesion and Adhesion Strengthening without Altering Soluble Ligand Binding. Mol. Biol. Cell 8: 2647-2657 [Abstract] [Full Text]
Brower, D. L., Brower, S. M., Hayward, D. C., Ball, E. E. (1997). Molecular evolution of integrins: Genes encoding integrin beta �subunits from a coral and a�sponge. Proc. Natl. Acad. Sci. USA 94: 9182-9187 [Abstract] [Full Text]
Takagi, J., Kamata, T., Meredith, J., Puzon-McLaughlin, W., Takada, Y. (1997). Changing Ligand Specificities of alpha vbeta 1 and alpha vbeta 3 Integrins by Swapping a Short Diverse Sequence of the beta �Subunit. J. Biol. Chem. 272: 19794-19800 [Abstract] [Full Text]
Irie, A., Kamata, T., Takada, Y. (1997). Multiple loop structures critical for ligand binding of the integrin alpha 4 subunit in the upper face of the beta -propeller mode�1. Proc. Natl. Acad. Sci. USA 94: 7198-7203 [Abstract] [Full Text]
Isobe, T., Hisaoka, T., Shimizu, A., Okuno, M., Aimoto, S., Takada, Y., Saito, Y., Takagi, J. (1997). Propolypeptide of von Willebrand Factor Is a Novel Ligand for Very Late Antigen-4 Integrin. J. Biol. Chem. 272: 8447-8453 [Abstract] [Full Text]
Cruz, M., Dalgard, C., Ignatius, M. (1997). Functional partitioning of beta1 integrins revealed by activating and inhibitory mAbs. J. Cell Sci. 110: 2647-2659 [Abstract]
Shih, D., Boettiger, D, Buck, C. (1997). Epitopes of adhesion-perturbing monoclonal antibodies map within a predicted alpha-helical domain of the integrin beta 1 subunit. J. Cell Sci. 110: 2619-2628 [Abstract]
Vignoud, L, Albiges-Rizo, C, Frachet, P, Block, M. (1997). NPXY motifs control the recruitment of the alpha5beta1 integrin in focal adhesions independently of the association of talin with the beta1 chain. J. Cell Sci. 110: 1421-1430 [Abstract]
Faull, R. J., Wang, J., Leavesley, D. I., Puzon, W., Russ, G. R., Vestweber, D., Takada, Y. (1996). A Novel Activating Anti-beta 1 Integrin Monoclonal Antibody Binds to the Cysteine-rich Repeats in the beta 1 Chain. J. Biol. Chem. 271: 25099-25106 [Abstract] [Full Text]
Goodman, T. G., Bajt, M. L. (1996). Identifying the Putative Metal Ion-dependent Adhesion Site in the beta 2 (CD18) Subunit Required for alpha Lbeta 2 and alpha Mbeta 2 Ligand Interactions. J. Biol. Chem. 271: 23729-23736 [Abstract] [Full Text]
Tozer, E. C., Liddington, R. C., Sutcliffe, M. J., Smeeton, A. H., Loftus, J. C. (1996). Ligand Binding to Integrin alpha IIbbeta 3 Is Dependent on a MIDAS-like Domain in the beta 3 Subunit. J. Biol. Chem. 271: 21978-21984 [Abstract] [Full Text]
Puzon-McLaughlin, W., Takada, Y. (1996). Critical Residues for Ligand Binding in an I Domain-like Structure of the Integrin beta 1 Subunit. J. Biol. Chem. 271: 20438-20443 [Abstract] [Full Text]
Puzon-McLaughlin, W., Yednock, T. A., Takada, Y. (1996). Regulation of Conformation and Ligand Binding Function of Integrin alpha 5beta 1 by the beta 1 Cytoplasmic Domain. J. Biol. Chem. 271: 16580-16585 [Abstract] [Full Text]
Loftus, J. C., Halloran, C. E., Ginsberg, M. H., Feigen, L. P., Zablocki, J. A., Smith, J. W. (1996). The Amino-terminal One-third of alpha[IMAGE] Defines the Ligand Recognition Specificity of Integrin alpha[IMAGE]beta(3). J. Biol. Chem. 271: 2033-2039 [Abstract] [Full Text]
Mould, A. (1996). Getting integrins into shape: recent insights into how integrin activity is regulated by conformational changes. J. Cell Sci. 109: 2613-2618
Bazzoni, G., Shih, D.-T., Buck, C. A., Hemler, M. E. (1995). Monoclonal Antibody 9EG7 Defines a Novel beta(1) Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium. J. Biol. Chem. 270: 25570-25577 [Abstract] [Full Text]
McGuire, S. L., Bajt, M. L. (1995). Distinct Ligand Binding Sites in the I Domain of Integrin alpha(M)beta(2) That Differentially Affect a Divalent Cation-dependent Conformation. J. Biol. Chem. 270: 25866-25871 [Abstract] [Full Text]
Fornaro, M., Zheng, D.-Q., Languino, L. R. (1995). The Novel Structural Motif Gln[IMAGE]-Gln[IMAGE] in the Integrin beta[IMAGE] Cytoplasmic Domain Regulates Cell Proliferation. J. Biol. Chem. 270: 24666-24669 [Abstract] [Full Text]
Meredith, J Jr, Takada, Y, Fornaro, M, Languino, L., Schwartz, M. (1995). Inhibition of cell cycle progression by the alternatively spliced integrin beta 1C. Science 269: 1570-1572 [Abstract]
Ma, L., Conrad, P. J., Webb, D. L., Blue, M.-L. (1995). Aspartate 698 within a Novel Cation Binding Motif in alpha(4) Integrin Is Required for Cell Adhesion. J. Biol. Chem. 270: 18401-18407 [Abstract] [Full Text]
Kamata, T., Wright, R., Takada, Y. (1995). Critical Threonine and Aspartic Acid Residues within the I Domains of [IMAGE]2 Integrins for Interactions with Intercellular Adhesion Molecule 1 (ICAM-1) and C3bi. J. Biol. Chem. 270: 12531-12535 [Abstract] [Full Text]
Bajt, M. L., Goodman, T., McGuire, S. L. (1995). beta(2)(CD18) Mutations Abolish Ligand Recognition by I Domain Integrins LFA-1 (alpha(L)beta(2), CD11a/CD18) and MAC-1 (alpha(M)beta(2), CD11b/CD18). J. Biol. Chem. 270: 94-98 [Abstract] [Full Text]
DiPersio, C., Shah, S, Hynes, R. (1995). alpha 3A beta 1 integrin localizes to focal contacts in response to diverse extracellular matrix proteins. J. Cell Sci. 108: 2321-2336 [Abstract]
Meijne, A., Casey, D., Feltkamp, C., Roos, E (1994). Immuno-EM localization of the beta 1 integrin subunit in wet-cleaved fibronectin-adherent fibroblasts. J. Cell Sci. 107: 1229-1239 [Abstract]
Higgins, J. M. G., Cernadas, M., Tan, K., Irie, A., Wang, J.-h., Takada, Y., Brenner, M. B. (2000). The Role of alpha and beta Chains in Ligand Recognition by beta 7 Integrins. J. Biol. Chem. 275: 25652-25664 [Abstract] [Full Text]
Eto, K., Puzon-McLaughlin, W., Sheppard, D., Sehara-Fujisawa, A., Zhang, X.-P., Takada, Y. (2000). RGD-independent Binding of Integrin alpha 9beta 1 to the ADAM-12 and -15 Disintegrin Domains Mediates Cell-Cell Interaction. J. Biol. Chem. 275: 34922-34930 [Abstract] [Full Text]
Tarui, T., Mazar, A. P., Cines, D. B., Takada, Y. (2001). Urokinase-type Plasminogen Activator Receptor (CD87) Is a Ligand for Integrins and Mediates Cell-Cell Interaction. J. Biol. Chem. 276: 3983-3990 [Abstract] [Full Text]