Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins

doi:10.1083/jcb.119.5.1047

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Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins

N Imamoto, Y Matsuoka, T Kurihara, K Kohno, M Miyagi, F Sakiyama, Y Okada, S Tsunasawa and Y Yoneda
Institute for Molecular and Cellular Biology, Osaka University, Japan.

Previously, we found that anti-DDDED antibodies strongly inhibited in vivo nuclear transport of nuclear proteins and that these antibodies recognized a protein of 69 kD (p69) from rat liver nuclear envelopes that showed specific binding activities to the nuclear location sequences (NLSs) of nucleoplasmin and SV-40 large T-antigen. Here we identified this protein as the 70-kD heat shock cognate protein (hsc70) based on its mass, isoelectric point, cellular localization, and partial amino acid sequences. Competition studies indicated that the recombinant hsc70 expressed in Escherichia coli binds to transport competent SV-40 T-antigen NLS more strongly than to the point mutated transport incompetent mutant NLS. To investigate the possible involvement of hsc70 in nuclear transport, we examined the effect of anti-hsc70 rabbit antibodies on the nuclear accumulation of karyophilic proteins. When injected into the cytoplasm of tissue culture cells, anti-hsc70 strongly inhibited the nuclear import of nucleoplasmin, SV- 40 T-antigen NLS bearing BSA and histone H1. In contrast, anti-hsc70 IgG did not prevent the diffusion of lysozyme or 17.4-kD FITC-dextran into the nuclei. After injection of these antibodies, cells continued RNA synthesis and were viable. These results indicate that hsc70 interacts with NLS-containing proteins in the cytoplasm before their nuclear import.
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Fourie, A. M., Hupp, T. R., Lane, D. P., Sang, B.-C., Barbosa, M. S., Sambrook, J. F., Gething, M.-J. H. (1997). HSP70 Binding Sites in the Tumor Suppressor Protein p53. J. Biol. Chem. 272: 19471-19479 [Abstract] [Full Text]
Kelley, W. L., Georgopoulos, C. (1997). The T/t common exon of simian virus 40,�JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular�chaperone. Proc. Natl. Acad. Sci. USA 94: 3679-3684 [Abstract] [Full Text]
Leung, S.-M., Hightower, L. E. (1997). A 16-kDa Protein Functions as a New Regulatory Protein for Hsc70 Molecular Chaperone and Is Identified as a Member of the Nm23/Nucleoside Diphosphate Kinase Family. J. Biol. Chem. 272: 2607-2614 [Abstract] [Full Text]
Buxbaum, E., Woodman, P. G. (1996). The speed of partial reactions of the uncoating ATPase Hsc70 depends on the source of coated vesicles. J. Cell Sci. 109: 705-711 [Abstract]
Takenaka, I. M., Leung, S.-M., McAndrew, S. J., Brown, J. P., Hightower, L. E. (1995). Hsc70-binding Peptides Selected from a Phage Display Peptide Library that Resemble Organellar Targeting Sequences. J. Biol. Chem. 270: 19839-19844 [Abstract] [Full Text]
Enenkel, C., Blobel, G�n., Rexach, M. (1995). Identification of a Yeast Karyopherin Heterodimer That Targets Import Substrate to Mammalian Nuclear Pore Complexes. J. Biol. Chem. 270: 16499-16502 [Abstract] [Full Text]
Saitoh, H., Dasso, M. (1995). The RCC1 Protein Interacts with Ran, RanBP1, hsc70, and a 340-kDa Protein in Xenopus Extracts. J. Biol. Chem. 270: 10658-10663 [Abstract] [Full Text]
Imamoto, N., Tachibana, T., Matsubae, M., Yoneda, Y. (1995). A Karyophilic Protein Forms a Stable Complex with Cytoplasmic Components Prior to Nuclear Pore Binding. J. Biol. Chem. 270: 8559-8565 [Abstract] [Full Text]
Liao, J., Lowthert, L. A., Ghori, N., Omary, M. B. (1995). The 70-kDa Heat Shock Proteins Associate with Glandular Intermediate Filaments in an ATP-dependent Manner. J. Biol. Chem. 270: 915-922 [Abstract] [Full Text]
Adam, S.A., Adam, E.J.H., Chi, N.C., Visser, G.D. (1995). Cytoplasmic Factors in NLS-mediated Targeting to the Nuclear Pore Complex. Cold Spring Harb Symp Quant Biol 60: 687-694 [Abstract]
Melchior, F., Guan, T., Paschal, B., Gerace, L. (1995). Biochemical and Structural Analysis of Nuclear Protein Import. Cold Spring Harb Symp Quant Biol 60: 707-716 [Abstract]
Shiina, N, Gotoh, Y, Kubomura, N, Iwamatsu, A, Nishida, E (1994). Microtubule severing by elongation factor 1 alpha. Science 266: 282-285 [Abstract]
Honing, S, Kreimer, G, Robenek, H, Jockusch, B. (1994). Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70). J. Cell Sci. 107: 1185-1196 [Abstract]
He, X., Bataille, N, Fried, H. (1994). Nuclear export of signal recognition particle RNA is a facilitated process that involves the Alu sequence domain. J. Cell Sci. 107: 903-912 [Abstract]
Akakura, S., Yoshida, M., Yoneda, Y., Horinouchi, S. (2001). A Role for Hsc70 in Regulating Nucleocytoplasmic Transport of a Temperature-sensitive p53 (p53Val-135). J. Biol. Chem. 276: 14649-14657 [Abstract] [Full Text]