Multiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes

doi:10.1083/jcb.119.5.1077

This Article

	Full Text (PDF, 6383K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Schwaninger, R.
	Articles by Balch, W. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Schwaninger, R.
	Articles by Balch, W. E.


Social Bookmarking

	What's this?

ARTICLES

Multiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes

R Schwaninger, H Plutner, GM Bokoch and WE Balch
Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037.

Using indirect immunofluorescence we have examined the effects of reagents which inhibit the function of ras-related rab small GTP- binding proteins and heterotrimeric G alpha beta gamma proteins in ER to Golgi transport. Export from the ER was inhibited by an antibody towards rab1B and an NH2-terminal peptide which inhibits ARF function (Balch, W. E., R. A. Kahn, and R. Schwaninger. 1992. J. Biol. Chem. 267:13053-13061), suggesting that both of these small GTP-binding proteins are essential for the transport vesicle formation. Export from the ER was also potently inhibited by mastoparan, a peptide which mimics G protein binding regions of seven transmembrane spanning receptors activating and uncoupling heterotrimeric G proteins from their cognate receptors. Consistent with this result, purified beta gamma subunits inhibited the export of VSV-G from the ER suggesting an initial event in transport vesicle assembly was regulated by a heterotrimeric G protein. In contrast, incubation in the presence of GTP gamma S or AIF(3-5) resulted in the accumulation of transported protein in different populations of punctate pre-Golgi intermediates distributed throughout the cytoplasm of the cell. Finally, a peptide which is believed to antagonize the interaction of rab proteins with putative downstream effector molecules inhibited transport at a later step preceding delivery to the cis Golgi compartment, similar to the site of accumulation of transported protein in the absence of NSF or calcium (Plutner, H., H. W. Davidson, J. Saraste, and W. E. Balch. 1992. J. Cell Biol. 119:1097-1116). These results are consistent with the hypothesis that multiple GTP-binding proteins including a heterotrimeric G protein(s), ARF and rab1 differentially regulate steps in the transport of protein between early compartments of the secretory pathway. The concept that G protein-coupled receptors gate the export of protein from the ER is discussed.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lu, L., Khan, A., Walker, W. A. (2009). ADP-ribosylation factors regulate the development of CT signaling in immature human enterocytes. Am. J. Physiol. Gastrointest. Liver Physiol. 296: G1221-G1229 [Abstract] [Full Text]
Groves, B., Gong, Q., Xu, Z., Huntsman, C., Nguyen, C., Li, D., Ma, D. (2007). A specific role of AGS3 in the surface expression of plasma membrane proteins. Proc. Natl. Acad. Sci. USA 104: 18103-18108 [Abstract] [Full Text]
Dipolo, R., Beauge, L. (2006). Sodium/Calcium Exchanger: Influence of Metabolic Regulation on Ion Carrier Interactions. Physiol. Rev. 86: 155-203 [Abstract] [Full Text]
Wu, G., Zhao, G., He, Y. (2003). Distinct Pathways for the Trafficking of Angiotensin II and Adrenergic Receptors from the Endoplasmic Reticulum to the Cell Surface: Rab1-INDEPENDENT TRANSPORT OF A G PROTEIN-COUPLED RECEPTOR. J. Biol. Chem. 278: 47062-47069 [Abstract] [Full Text]
Litovchick, L., Friedmann, E., Shaltiel, S. (2002). A Selective Interaction between OS-9 and the Carboxyl-terminal Tail of Meprin beta. J. Biol. Chem. 277: 34413-34423 [Abstract] [Full Text]
Ferrezuelo, F., Steiner, B., Aldea, M., Futcher, B. (2002). Biogenesis of Yeast Telomerase Depends on the Importin Mtr10. Mol. Cell. Biol. 22: 6046-6055 [Abstract] [Full Text]
Nagahama, M., Usui, S., Shinohara, T., Yamaguchi, T., Tani, K., Tagaya, M. (2002). Inactivation of G{alpha}z causes disassembly of the Golgi apparatus. J. Cell Sci. 115: 4483-4493 [Abstract] [Full Text]
Chen, S. S.-L., Lee, S.-F., Wang, C.-T. (2001). Cellular Membrane-Binding Ability of the C-Terminal Cytoplasmic Domain of Human Immunodeficiency Virus Type 1 Envelope Transmembrane Protein gp41. J. Virol. 75: 9925-9938 [Abstract] [Full Text]
Ono, A., Orenstein, J. M., Freed, E. O. (2000). Role of the Gag Matrix Domain in Targeting Human Immunodeficiency Virus Type 1 Assembly. J. Virol. 74: 2855-2866 [Abstract] [Full Text]
Alvarez, C., Fujita, H., Hubbard, A., Sztul, E. (1999). ER to Golgi Transport: Requirement for P115 at a Pre-Golgi Vtc Stage. JCB 147: 1205-1222 [Abstract] [Full Text]
Andersson, H., Kappeler, F., Hauri, H.-P. (1999). Protein Targeting to Endoplasmic Reticulum by Dilysine Signals Involves Direct Retention in Addition to Retrieval. J. Biol. Chem. 274: 15080-15084 [Abstract] [Full Text]
Ueno, T., Ishidoh, K., Mineki, R., Tanida, I., Murayama, K., Kadowaki, M., Kominami, E. (1999). Autolysosomal Membrane-associated Betaine Homocysteine Methyltransferase. LIMITED DEGRADATION FRAGMENT OF A SEQUESTERED CYTOSOLIC ENZYME MONITORING AUTOPHAGY. J. Biol. Chem. 274: 15222-15229 [Abstract] [Full Text]
Majoul, I., Sohn, K., Wieland, F. T., Pepperkok, R., Pizza, M., Hillemann, J., Soling, H.-D. (1998). KDEL Receptor (Erd2p)-mediated Retrograde Transport of the Cholera Toxin A Subunit from the Golgi Involves COPI, p23, and the COOH Terminus of Erd2p. JCB 143: 601-612 [Abstract] [Full Text]
Smine, A., Xu, X., Nishiyama, K., Katada, T., Gambetti, P., Yadav, S. P., Wu, X., Shi, Y.-C., Yasuhara, S., Homburger, V., Okamoto, T. (1998). Regulation of Brain G-protein Go by Alzheimer's Disease Gene Presenilin-1. J. Biol. Chem. 273: 16281-16288 [Abstract] [Full Text]
Helms, J. B., Helms-Brons, D., Brugger, B., Gkantiragas, I., Eberle, H., Nickel, W., Nurnberg, B., Gerdes, H.-H., Wieland, F. T. (1998). A Putative Heterotrimeric G Protein Inhibits the Fusion of COPI-coated Vesicles. SEGREGATION OF HETEROTRIMERIC G PROTEINS FROM COPI-COATED VESICLES. J. Biol. Chem. 273: 15203-15208 [Abstract] [Full Text]
Yang, W., Storrie, B. (1998). Scattered Golgi Elements during Microtubule Disruption Are Initially Enriched in Trans-Golgi Proteins. Mol. Biol. Cell 9: 191-207 [Abstract] [Full Text]
Kappeler, F., Klopfenstein, D. R.�Ch., Foguet, M., Paccaud, J.-P., Hauri, H.-P. (1997). The Recycling of ERGIC-53 in the Early Secretory Pathway. ERGIC-53 CARRIES A CYTOSOLIC ENDOPLASMIC RETICULUM-EXIT DETERMINANT INTERACTING WITH COPII. J. Biol. Chem. 272: 31801-31808 [Abstract] [Full Text]
Kind, P. C., Kelly, G. M., Fryer, H. J.�L., Blakemore, C., Hockfield, S. (1997). Phospholipase C-beta 1 Is Present in the Botrysome, an Intermediate Compartment-Like Organelle, and Is Regulated by Visual Experience in Cat Visual Cortex. J. Neurosci. 17: 1471-1480 [Abstract] [Full Text]
Tisdale, E. J., Balch, W. E. (1996). Rab2 Is Essential for the Maturation of Pre-Golgi Intermediates. J. Biol. Chem. 271: 29372-29379 [Abstract] [Full Text]
Ogier-Denis, E., Houri, J.-J., Bauvy, C., Codogno, P. (1996). Guanine Nucleotide Exchange on Heterotrimeric Gi3 Protein Controls Autophagic Sequestration in HT-29 Cells. J. Biol. Chem. 271: 28593-28600 [Abstract] [Full Text]
Schnitzer, J. E., Oh, P., McIntosh, D. P. (1996). Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes. Science 274: 239-242 [Abstract] [Full Text]
Buccione, R., Bannykh, S., Santone, I., Baldassarre, M., Facchiano, F., Bozzi, Y., Di Tullio, G., Mironov, A., Luini, A., De Matteis, M. A. (1996). Regulation of Constitutive Exocytic Transport by Membrane Receptors. J. Biol. Chem. 271: 3523-3533 [Abstract] [Full Text]
Lowe, S., Wong, S., Hong, W (1996). The mammalian ARF-like protein 1 (Arl1) is associated with the Golgi complex. J. Cell Sci. 109: 209-220 [Abstract]
Smith, T., Ferreira, L. R., Hebert, C., Norris, K., Sauk, J. J. (1995). Hsp47 and Cyclophilin B Traverse the Endoplasmic Reticulum with Procollagen into Pre-Golgi Intermediate Vesicles. J. Biol. Chem. 270: 18323-18328 [Abstract] [Full Text]
Dugan, J. M., deWit, C., McConlogue, L., Maltese, W. A. (1995). The Ras-related GTP-binding Protein, Rab1B, Regulates Early Steps in Exocytic Transport and Processing of [IMAGE]-Amyloid Precursor Protein. J. Biol. Chem. 270: 10982-10989 [Abstract] [Full Text]
Ogier-Denis, E., Couvineau, A., Maoret, J. J., Houri, J. J., Bauvy, C., De Stefanis, D., Isidoro, C., Laburthe, M., Codogno, P. (1995). A Heterotrimeric G[IMAGE]-protein Controls Autophagic Sequestration in the Human Colon Cancer Cell Line HT-29. J. Biol. Chem. 270: 13-16 [Abstract] [Full Text]
Saraste, J, Lahtinen, U, Goud, B (1995). Localization of the small GTP-binding protein rab1p to early compartments of the secretory pathway. J. Cell Sci. 108: 1541-1552 [Abstract]
Brewer, C., Roth, M. (1995). Polarized exocytosis in MDCK cells is regulated by phosphorylation. J. Cell Sci. 108: 789-796 [Abstract]
Bannykh, S., Aridor, M., Plutner, H., Rowe, T., Balch, W.E. (1995). Regulated Export of Cargo from the Endoplasmic Reticulum of Mammalian Cells. Cold Spring Harb Symp Quant Biol 60: 127-137 [Abstract]
Montmayeur, J., Borrelli, E (1994). Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region. Science 263: 95-98 [Abstract]