Nerve growth factor withdrawal-induced cell death in neuronal PC12 cells resembles that in sympathetic neurons

doi:10.1083/jcb.119.6.1669

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Nerve growth factor withdrawal-induced cell death in neuronal PC12 cells resembles that in sympathetic neurons

PW Mesner, TR Winters and SH Green
Department of Biology, University of Iowa, Iowa City 52242.

Previous studies have shown that in neuronal cells the developmental phenomenon of programmed cell death is an active process, requiring synthesis of both RNA and protein. This presumably reflects a requirement for novel gene products to effect cell death. It is shown here that the death of nerve growth factor-deprived neuronal PC12 cells occurs at the same rate as that of rat sympathetic neurons and, like rat sympathetic neurons, involves new transcription and translation. In nerve growth factor-deprived neuronal PC12 cells, a decline in metabolic activity, assessed by uptake of [3H]2-deoxyglucose, precedes the decline in cell number, assessed by counts of trypan blue-excluding cells. Both declines are prevented by actinomycin D and anisomycin. In contrast, the death of nonneuronal (chromaffin-like) PC12 cells is not inhibited by transcription or translation inhibitors and thus does not require new protein synthesis. DNA fragmentation by internucleosomal cleavage does not appear to be a consistent or significant aspect of cell death in sympathetic neurons, neuronal PC12 cells, or nonneuronal PC12 cells, notwithstanding that the putative nuclease inhibitor aurintricarboxylic acid protects sympathetic neurons, as well as neuronal and nonneuronal PC12 cells, from death induced by trophic factor removal. Both phenotypic classes of PC12 cells respond to aurintricarboxylic acid with similar dose-response characteristics. Our results indicate that programmed cell death in neuronal PC12 cells, but not in nonneuronal PC12 cells, resembles programmed cell death in sympathetic neurons in significant mechanistic aspects: time course, role of new protein synthesis, and lack of a significant degree of DNA fragmentation.
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Goldshmit, Y., Erlich, S., Pinkas-Kramarski, R. (2001). Neuregulin Rescues PC12-ErbB4 Cells from Cell Death Induced by H2O2. REGULATION OF REACTIVE OXYGEN SPECIES LEVELS BY PHOSPHATIDYLINOSITOL 3-KINASE. J. Biol. Chem. 276: 46379-46385 [Abstract] [Full Text]
De Zutter, G. S., Davis, R. J. (2001). Pro-apoptotic gene expression mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Proc. Natl. Acad. Sci. USA 10.1073/pnas.111027698v1 [Abstract] [Full Text]
Niikura, T., Hashimoto, Y., Okamoto, T., Abe, Y., Yasukawa, T., Kawasumi, M., Hiraki, T., Kita, Y., Terashita, K., Kouyama, K., Nishimoto, I. (2001). Insulin-Like Growth Factor I (IGF-I) Protects Cells from Apoptosis by Alzheimer's V642I Mutant Amyloid Precursor Protein through IGF-I Receptor in an IGF-Binding Protein-Sensitive Manner. J. Neurosci. 21: 1902-1910 [Abstract] [Full Text]
Chuenkova, M. V., Pereira, M. A. (2000). A Trypanosomal Protein Synergizes with the Cytokines Ciliary Neurotrophic Factor and Leukemia Inhibitory Factor to Prevent Apoptosis of Neuronal Cells. Mol. Biol. Cell 11: 1487-1498 [Abstract] [Full Text]
Guardavaccaro, D., Corrente, G., Covone, F., Micheli, L., D'Agnano, I., Starace, G., Caruso, M., Tirone, F. (2000). Arrest of G1-S Progression by the p53-Inducible Gene PC3 Is Rb Dependent and Relies on the Inhibition of Cyclin D1 Transcription. Mol. Cell. Biol. 20: 1797-1815 [Abstract] [Full Text]
Persengiev, S. P., Kondova, I. I., Kilpatrick, D. L. (1999). E2F4 Actively Promotes the Initiation and Maintenance of Nerve Growth Factor-Induced Cell Differentiation. Mol. Cell. Biol. 19: 6048-6056 [Abstract] [Full Text]
Slinskey, A., Barnes, D., Pipas, J. M. (1999). Simian Virus 40 Large T Antigen J Domain and Rb-Binding Motif Are Sufficient To Block Apoptosis Induced by Growth Factor Withdrawal in a Neural Stem Cell Line. J. Virol. 73: 6791-6799 [Abstract] [Full Text]
Grønborg, M., Johansen, T. E., Peters, D., Ahring, P. K., Drejer, J., Møller, A., Jensen, L. H. (1999). Neuroprotection by a Novel Compound, NS521. J. Pharmacol. Exp. Ther. 290: 348-353 [Abstract] [Full Text]
Fujio, Y., Walsh, K. (1999). Akt Mediates Cytoprotection of Endothelial Cells by Vascular Endothelial Growth Factor in an Anchorage-dependent Manner. J. Biol. Chem. 274: 16349-16354 [Abstract] [Full Text]
Stefanis, L., Troy, C. M., Qi, H., Shelanski, M. L., Greene, L. A. (1998). Caspase-2 (Nedd-2) Processing and Death of Trophic Factor-Deprived PC12 Cells and Sympathetic Neurons Occur Independently of Caspase-3 (CPP32)-Like Activity. J. Neurosci. 18: 9204-9215 [Abstract] [Full Text]
Farinelli, S. E., Greene, L. A., Friedman, W. J. (1998). Neuroprotective Actions of Dipyridamole on Cultured CNS Neurons. J. Neurosci. 18: 5112-5123 [Abstract] [Full Text]
Graves, J. D., Draves, K. E., Craxton, A., Krebs, E. G., Clark, E. A. (1998). A Comparison of Signaling Requirements for Apoptosis of Human B Lymphocytes Induced by the B Cell Receptor and CD95/Fas. J. Immunol. 161: 168-174 [Abstract] [Full Text]
Aronica, E. M., Gorter, J. A., Grooms, S., Kessler, J. A., Bennett, M. V.�L., Zukin, R. S., Rosenbaum, D. M. (1998). Aurintricarboxylic acid prevents GLUR2 mRNA down-regulation and delayed neurodegeneration in hippocampal CA1 neurons of gerbil after global ischemia. Proc. Natl. Acad. Sci. USA 95: 7115-7120 [Abstract] [Full Text]
Wadia, J. S., Chalmers-Redman, R. M.�E., Ju, W. J.�H., Carlile, G. W., Phillips, J. L., Fraser, A. D., Tatton, W. G. (1998). Mitochondrial Membrane Potential and Nuclear Changes in Apoptosis Caused by Serum and Nerve Growth Factor Withdrawal: Time Course and Modification by (-)-Deprenyl. J. Neurosci. 18: 932-947 [Abstract] [Full Text]
Songyang, Z., Baltimore, D., Cantley, L. C., Kaplan, D. R., Franke, T. F. (1997). Interleukin 3-dependent survival by the Akt protein�kinase. Proc. Natl. Acad. Sci. USA 94: 11345-11350 [Abstract] [Full Text]
Grimes, M. L., Beattie, E., Mobley, W. C. (1997). A signaling organelle containing the nerve growth factor-activated receptor tyrosine kinase, TrkA. Proc. Natl. Acad. Sci. USA 94: 9909-9914 [Abstract] [Full Text]
Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M. (1997). Molecular Cloning of a Novel Polypeptide, DP5, Induced during Programmed Neuronal Death. J. Biol. Chem. 272: 18842-18848 [Abstract] [Full Text]
Deshmukh, M., Johnson, E. M. Jr. (1997). Programmed Cell Death in Neurons: Focus on the Pathway of Nerve Growth Factor Deprivation-Induced Death of Sympathetic Neurons. Mol. Pharmacol. 51: 897-906 [Abstract] [Full Text]
Troy, C. M., Stefanis, L., Greene, L. A., Shelanski, M. L. (1997). Nedd2 Is Required for Apoptosis after Trophic Factor Withdrawal, But Not Superoxide Dismutase (SOD1) Downregulation, in Sympathetic Neurons and PC12 Cells. J. Neurosci. 17: 1911-1918 [Abstract] [Full Text]
D'Mello, S. R., Borodezt, K., Soltoff, S. P. (1997). Insulin-Like Growth Factor and Potassium Depolarization Maintain Neuronal Survival by Distinct Pathways: Possible Involvement of PI 3-Kinase in IGF-1 Signaling. J. Neurosci. 17: 1548-1560 [Abstract] [Full Text]
Park, D. S., Morris, E. J., Greene, L. A., Geller, H. M. (1997). G1/S Cell Cycle Blockers and Inhibitors of Cyclin-Dependent Kinases Suppress Camptothecin-Induced Neuronal Apoptosis. J. Neurosci. 17: 1256-1270 [Abstract] [Full Text]
Stefanis, L., Park, D. S., Yan, C. Y. I., Farinelli, S. E., Troy, C. M., Shelanski, M. L., Greene, L. A. (1996). Induction of CPP32-like Activity in PC12 Cells by Withdrawal of Trophic Support. DISSOCIATION FROM APOPTOSIS. J. Biol. Chem. 271: 30663-30671 [Abstract] [Full Text]
Park, D. S., Stefanis, L., Yan, C. Y. I., Farinelli, S. E., Greene, L. A. (1996). Ordering the Cell Death Pathway. DIFFERENTIAL EFFECTS OF BCL2, AN INTERLEUKIN-1-CONVERTING ENZYME FAMILY PROTEASE INHIBITOR, AND OTHER SURVIVAL AGENTS ON JNK ACTIVATION IN SERUM/NERVE GROWTH FACTOR-DEPRIVED PC12 CELLS. J. Biol. Chem. 271: 21898-21905 [Abstract] [Full Text]
Park, D. S., Farinelli, S. E., Greene, L. A. (1996). Inhibitors of Cyclin-dependent Kinases Promote Survival of Post-mitotic Neuronally Differentiated PC12 Cells and Sympathetic Neurons. J. Biol. Chem. 271: 8161-8169 [Abstract] [Full Text]
Xia, Z., Dickens, M., Raingeaud, J.�l, Davis, R. J., Greenberg, M. E. (1995). Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis. Science 270: 1326-1331 [Abstract]
Yan, C. Y. I., Ferrari, G., Greene, L. A. (1995). N-Acetylcysteine-promoted Survival of PC12 Cells Is Glutathione-independent but Transcription-dependent. J. Biol. Chem. 270: 26827-26832 [Abstract] [Full Text]
Hossain, M. Md., Nakayama, H., Goto, N. (1995). Apoptosis in the Central Nervous System of Developing Mouse Fetuses from 5-Azacytidine-Administered Dams. Toxicol Pathol 23: 367-372 [Abstract]
Anastasiadis, P. Z., Jiang, H., Bezin, L., Kuhn, D. M., Levine, R. A. (2001). Tetrahydrobiopterin Enhances Apoptotic PC12 Cell Death following Withdrawal of Trophic Support. J. Biol. Chem. 276: 9050-9058 [Abstract] [Full Text]
De Zutter, G. S., Davis, R. J. (2001). Pro-apoptotic gene expression mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Proc. Natl. Acad. Sci. USA 98: 6168-6173 [Abstract] [Full Text]