Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility

doi:10.1083/jcb.120.1.163

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Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility

EL de Hostos, C Rehfuess, B Bradtke, DR Waddell, R Albrecht, J Murphy and G Gerisch
Max-Planck-Institut fur Biochemie, Martinsried, Germany.

Coronin is an actin-binding protein in Dictyostelium discoideum that is enriched at the leading edge of the cells and in projections of the cell surface called crowns. The polypeptide sequence of coronin is distinguished by its similarities to the beta-subunits of trimeric G proteins (E. L. de Hostos, B. Bradtke, F. Lottspeich, R. Guggenheim, and G. Gerisch, 1991. EMBO (Eur. Mol. Biol. Organ.) J. 10:4097-4104). To elucidate the in vivo function of coronin, null mutants have been generated by gene replacement. The mutant cells lacking coronin grow and migrate more slowly than wild-type cells. When these cor- cells grow in liquid medium they become multinucleate, indicating a role of coronin in cytokinesis. To explore this role, coronin has been localized in mitotic wild-type cells by immunofluorescence labeling. During separation of the daughter cells, coronin is strongly accumulated at their distal portions including the leading edges. This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis.
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Oku, T., Kaneko, Y., Murofushi, K., Seyama, Y., Toyoshima, S., Tsuji, T. (2008). Phorbol Ester-dependent Phosphorylation Regulates the Association of p57/Coronin-1 with the Actin Cytoskeleton. J. Biol. Chem. 283: 28918-28925 [Abstract] [Full Text]
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Ahmed, Z., Shaw, G., Sharma, V. P., Yang, C., McGowan, E., Dickson, D. W. (2007). Actin-binding Proteins Coronin-1a and IBA-1 Are Effective Microglial Markers for Immunohistochemistry. J. Histochem. Cytochem. 55: 687-700 [Abstract] [Full Text]
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Caracino, D., Jones, C., Compton, M., Saxe, C. L. III (2007). The N-Terminus of Dictyostelium Scar Interacts with Abi and HSPC300 and Is Essential for Proper Regulation and Function. Mol. Biol. Cell 18: 1609-1620 [Abstract] [Full Text]
Octtaviani, E., Effler, J. C., Robinson, D. N. (2006). Enlazin, a Natural Fusion of Two Classes of Canonical Cytoskeletal Proteins, Contributes to Cytokinesis Dynamics. Mol. Biol. Cell 17: 5275-5286 [Abstract] [Full Text]
Foger, N., Rangell, L., Danilenko, D. M., Chan, A. C. (2006). Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis.. Science 313: 839-842 [Abstract] [Full Text]
Sheppard, F. R., Kelher, M. R., Moore, E. E., McLaughlin, N. J. D., Banerjee, A., Silliman, C. C. (2005). Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation. J. Leukoc. Biol. 78: 1025-1042 [Abstract] [Full Text]
Cai, L., Holoweckyj, N., Schaller, M. D., Bear, J. E. (2005). Phosphorylation of Coronin 1B by Protein Kinase C Regulates Interaction with Arp2/3 and Cell Motility. J. Biol. Chem. 280: 31913-31923 [Abstract] [Full Text]
Bakal, C. J., Finan, D., LaRose, J., Wells, C. D., Gish, G., Kulkarni, S., DeSepulveda, P., Wilde, A., Rottapel, R. (2005). The Rho GTP exchange factor Lfc promotes spindle assembly in early mitosis. Proc. Natl. Acad. Sci. USA 102: 9529-9534 [Abstract] [Full Text]
Yan, M., Collins, R. F., Grinstein, S., Trimble, W. S. (2005). Coronin-1 Function Is Required for Phagosome Formation. Mol. Biol. Cell 16: 3077-3087 [Abstract] [Full Text]
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Bharathi, V., Pallavi, S. K., Bajpai, R., Emerald, B. S., Shashidhara, L. S. (2004). Genetic characterization of the Drosophila homologue of coronin. J. Cell Sci. 117: 1911-1922 [Abstract] [Full Text]
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Tolliday, N., Pitcher, M., Li, R. (2003). Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic Mechanisms in the Absence of Myosin II. Mol. Biol. Cell 14: 798-809 [Abstract] [Full Text]
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Humphries, C. L., Balcer, H. I., D'Agostino, J. L., Winsor, B., Drubin, D. G., Barnes, G., Andrews, B. J., Goode, B. L. (2002). Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin. JCB 159: 993-1004 [Abstract] [Full Text]
Spoerl, Z., Stumpf, M., Noegel, A. A., Hasse, A. (2002). Oligomerization, F-actin Interaction, and Membrane Association of the Ubiquitous Mammalian Coronin 3 Are Mediated by Its Carboxyl Terminus. J. Biol. Chem. 277: 48858-48867 [Abstract] [Full Text]
Nagasaki, A., de Hostos, E. L., Uyeda, T. Q. P. (2002). Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostelium. J. Cell Sci. 115: 2241-2251 [Abstract] [Full Text]
Robinson, D. N., Ocon, S. S., Rock, R. S., Spudich, J. A. (2002). Dynacortin Is a Novel Actin Bundling Protein That Localizes to Dynamic Actin Structures. J. Biol. Chem. 277: 9088-9095 [Abstract] [Full Text]
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Neuhaus, E. M., Soldati, T. (2000). A Myosin I Is Involved in Membrane Recycling from Early Endosomes. JCB 150: 1013-1026 [Abstract] [Full Text]
Robinson, D. N., Spudich, J. A. (2000). Dynacortin, a Genetic Link between Equatorial Contractility and Global Shape Control Discovered by Library Complementation of a Dictyostelium discoideum Cytokinesis Mutant. JCB 150: 823-838 [Abstract] [Full Text]
Solomon, J. M., Rupper, A., Cardelli, J. A., Isberg, R. R. (2000). Intracellular Growth of Legionella pneumophila in Dictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions. Infect. Immun. 68: 2939-2947 [Abstract] [Full Text]
Noegel, A., Schleicher, M (2000). The actin cytoskeleton of Dictyostelium: a story told by mutants. J. Cell Sci. 113: 759-766 [Abstract]
Schwarz, E., Neuhaus, E., Kistler, C, Henkel, A., Soldati, T (2000). Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis. J. Cell Sci. 113: 621-633 [Abstract]
Chubb, J., Wilkins, A, Thomas, G., Insall, R. (2000). The Dictyostelium RasS protein is required for macropinocytosis, phagocytosis and the control of cell movement. J. Cell Sci. 113: 709-719 [Abstract]
Deery, W. J., Gomer, R. H. (1999). A Putative Receptor Mediating Cell-density Sensing in Dictyostelium. J. Biol. Chem. 274: 34476-34482 [Abstract] [Full Text]
Pollock, N., de Hostos, E. L., Turck, C. W., Vale, R. D. (1999). Reconstitution of Membrane Transport Powered by a Novel Dimeric Kinesin Motor of the Unc104/Kif1a Family Purified from Dictyostelium. JCB 147: 493-506 [Abstract] [Full Text]
Rappleye, C. A., Paredez, A. R., Smith, C. W., McDonald, K. L., Aroian, R. V. (1999). The coronin-like protein POD-1 is required for anterior-posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans. Genes Dev. 13: 2838-2851 [Abstract] [Full Text]
Konzok, A., Weber, I., Simmeth, E., Hacker, U., Maniak, M., Muller-Taubenberger, A. (1999). Daip1, a Dictyostelium Homologue of the Yeast Actin-Interacting Protein 1, Is Involved in Endocytosis, Cytokinesis, and Motility. JCB 146: 453-464 [Abstract] [Full Text]
Allen, L.-A. H., DeLeo, F. R., Gallois, A., Toyoshima, S., Suzuki, K., Nauseef, W. M. (1999). Transient Association of the Nicotinamide Adenine Dinucleotide Phosphate Oxidase Subunits p47phox and p67phox With Phagosomes in Neutrophils From Patients With X-Linked Chronic Granulomatous Disease. Blood 93: 3521-3530 [Abstract] [Full Text]
Nakamura, T., Takeuchi, K., Muraoka, S., Takezoe, H., Takahashi, N., Mori, N. (1999). A Neurally Enriched Coronin-like Protein, ClipinC, Is a Novel Candidate for an Actin Cytoskeleton-Cortical Membrane-linking Protein. J. Biol. Chem. 274: 13322-13327 [Abstract] [Full Text]
Parente, J. A. Jr., Chen, X., Zhou, C., Petropoulos, A. C., Chew, C. S. (1999). Isolation, Cloning, and Characterization of a New Mammalian Coronin Family Member, Coroninse, Which Is Regulated within the Protein Kinase C Signaling Pathway. J. Biol. Chem. 274: 3017-3025 [Abstract] [Full Text]
Morrissette, N., Gold, E., Guo, J, Hamerman, J., Ozinsky, A, Bedian, V, Aderem, A. (1999). Isolation and characterization of monoclonal antibodies directed against novel components of macrophage phagosomes. J. Cell Sci. 112: 4705-4713 [Abstract]
Goode, B. L., Wong, J. J., Butty, A.-C., Peter, M., McCormack, A. L., Yates, J. R., Drubin, D. G., Barnes, G. (1999). Coronin Promotes the Rapid Assembly and Cross-linking of Actin Filaments and May Link the Actin and Microtubule Cytoskeletons in Yeast. JCB 144: 83-98 [Abstract] [Full Text]
Mishima, M, Nishida, E (1999). Coronin localizes to leading edges and is involved in cell spreading and lamellipodium extension in vertebrate cells. J. Cell Sci. 112: 2833-2842 [Abstract]
de Hostos, E. L., McCaffrey, G., Sucgang, R., Pierce, D. W., Vale, R. D. (1998). A Developmentally Regulated Kinesin-related Motor Protein from Dictyostelium discoideum. Mol. Biol. Cell 9: 2093-2106 [Abstract] [Full Text]
Neujahr, R., Heizer, C., Albrecht, R., Ecke, M., Schwartz, J.-M., Weber, I., Gerisch, G. (1997). Three-dimensional Patterns and Redistribution of Myosin II and Actin in Mitotic Dictyostelium Cells. JCB 139: 1793-1804 [Abstract] [Full Text]
Zang, J.-H., Cavet, G., Sabry, J. H., Wagner, P., Moores, S. L., Spudich, J. A. (1997). On the Role of Myosin-II in Cytokinesis: Division of Dictyostelium Cells under Adhesive and Nonadhesive Conditions. Mol. Biol. Cell 8: 2617-2629 [Abstract] [Full Text]
Tuxworth, R. I., Cheetham, J. L., Machesky, L. M., Spiegelmann, G. B., Weeks, G., Insall, R. H. (1997). Dictyostelium RasG Is Required for Normal Motility and Cytokinesis, But Not Growth. JCB 138: 605-614 [Abstract] [Full Text]
Niswonger, M. L., O'Halloran, T. J. (1997). A novel role for clathrin in�cytokinesis. Proc. Natl. Acad. Sci. USA 94: 8575-8578 [Abstract] [Full Text]
Adachi, H., Takahashi, Y., Hasebe, T., Shirouzu, M., Yokoyama, S., Sutoh, K. (1997). Dictyostelium IQGAP-related Protein Specifically Involved in the Completion of Cytokinesis. JCB 137: 891-898 [Abstract] [Full Text]
Grogan, A, Reeves, E, Keep, N, Wientjes, F, Totty, N., Burlingame, A., Hsuan, J., Segal, A. (1997). Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils. J. Cell Sci. 110: 3071-3081 [Abstract]
Hacker, U, Albrecht, R, Maniak, M (1997). Fluid-phase uptake by macropinocytosis in Dictyostelium. J. Cell Sci. 110: 105-112 [Abstract]
Neujahr, R, Heizer, C, Gerisch, G (1997). Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow. J. Cell Sci. 110: 123-137 [Abstract]
Rivero, F, Koppel, B, Peracino, B, Bozzaro, S, Siegert, F, Weijer, C., Schleicher, M, Albrecht, R, Noegel, A. (1996). The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development. J. Cell Sci. 109: 2679-2691 [Abstract]
Milne, J. L. S., Wu, L., Caterina, M. J., Devreotes, P. N. (1995). Seven Helix cAMP Receptors Stimulate Ca[IMAGE] Entry in the Absence of Functional G Proteins in Dictyostelium. J. Biol. Chem. 270: 5926-5931 [Abstract] [Full Text]
Neuhaus, E. M., Almers, W., Soldati, T. (2002). Morphology and Dynamics of the Endocytic Pathway in Dictyostelium discoideum. Mol. Biol. Cell 13: 1390-1407 [Abstract] [Full Text]