Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells

doi:10.1083/jcb.120.1.5

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Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells

T Nilsson, M Pypaert, MH Hoe, P Slusarewicz, EG Berger and G Warren
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.

Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N- acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans- Golgi network but, contrary to expectation, NAGT I was found both in the medial- and trans-cisternae, overlapping the distribution of GalT. About one third of the NAGT I and half of the GalT were found in the shared, trans-cisterna. These data show that the differences between cisternae are determined not by different sets of enzymes but by different mixtures.
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