end3 and end4: two mutants defective in receptor-mediated and fluid- phase endocytosis in Saccharomyces cerevisiae

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end3 and end4: two mutants defective in receptor-mediated and fluid- phase endocytosis in Saccharomyces cerevisiae

S Raths, J Rohrer, F Crausaz and H Riezman
Biocenter, University of Basel, Switzerland.

alpha-factor, one of two peptide hormones responsible for synchronized mating between MATa and MAT alpha-cell types in Saccharomyces cerevisiae, binds to its cell surface receptor and is internalized in a time-, temperature-, and energy-dependent manner (Chvatchko, Y., I. Howald, and H. Riezman. 1986. Cell. 46:355-364). After internalization, alpha-factor is delivered to the vacuole via vesicular intermediates and degraded there consistent with an endocytic mechanism (Singer, B., and H. Riezman. 1990. J. Cell Biol. 110:1911-1922; Chvatchko, Y., I. Howald, and H. Riezman. 1986. Cell. 46:355-364). We have isolated two mutants that are defective in the internalization process. Both mutations confer a recessive, temperature-sensitive growth phenotype upon cells that cosegregates with their endocytosis defect. Lucifer yellow, a marker for fluid-phase endocytosis, shows accumulation characteristics in the mutants that are similar to the uptake characteristics of 35S-alpha-factor. The endocytic defect in end4 cells appears immediately upon shift to restrictive temperature and is reversible at permissive temperature if new protein synthesis is allowed. Furthermore, the end4 mutation only affects alpha-factor internalization and not the later delivery of alpha-factor to the vacuole. Other vesicle-mediated processes seem to be normal in end3 and end4 mutants. END3 and END4 are the first genes shown to be necessary for the internalization step of receptor-borne and fluid-phase markers in yeast.
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Wang, G., McCaffery, J. M., Wendland, B., Dupré, S., Haguenauer-Tsapis, R., Huibregtse, J. M. (2001). Localization of the Rsp5p Ubiquitin-Protein Ligase at Multiple Sites within the Endocytic Pathway. Mol. Cell. Biol. 21: 3564-3575 [Abstract] [Full Text]
Kessels, M. M., Engqvist-Goldstein, A. E.Y., Drubin, D. G., Qualmann, B. (2001). Mammalian Abp1, a Signal-Responsive F-Actin-Binding Protein, Links the Actin Cytoskeleton to Endocytosis via the Gtpase Dynamin. JCB 153: 351-366 [Abstract] [Full Text]
Losko, S., Kopp, F., Kranz, A., Kölling, R. (2001). Uptake of the ATP-Binding Cassette (ABC) Transporter Ste6 into the Yeast Vacuole Is Blocked in the doa4 Mutant. Mol. Biol. Cell 12: 1047-1059 [Abstract] [Full Text]
Asleson, C. M., Bensen, E. S., Gale, C. A., Melms, A.-S., Kurischko, C., Berman, J. (2001). Candida albicans INT1-Induced Filamentation in Saccharomyces cerevisiae Depends on Sla2p. Mol. Cell. Biol. 21: 1272-1284 [Abstract] [Full Text]
Whitacre, J. L., Davis, D. A., Toenjes, K. A., Brower, S. M., Adams, A. E. M. (2001). Generation of an Isogenic Collection of Yeast Actin Mutants and Identification of Three Interrelated Phenotypes. Genetics 157: 533-543 [Abstract] [Full Text]
Feng, Y., Davis, N. G. (2000). Akr1p and the Type I Casein Kinases Act prior to the Ubiquitination Step of Yeast Endocytosis: Akr1p Is Required for Kinase Localization to the Plasma Membrane. Mol. Cell. Biol. 20: 5350-5359 [Abstract] [Full Text]
Wiederkehr, A., Avaro, S., Prescianotto-Baschong, C., Haguenauer-Tsapis, R., Riezman, H. (2000). The F-Box Protein Rcy1p Is Involved in Endocytic Membrane Traffic and Recycling Out of an Early Endosome in Saccharomyces cerevisiae. JCB 149: 397-410 [Abstract] [Full Text]
Metzler, M., Helgason, C. D., Dragatsis, I., Zhang, T., Gan, L., Pineault, N., Zeitlin, S. O., Humphries, R. K., Hayden, M. R. (2000). Huntingtin is required for normal hematopoiesis. Hum Mol Genet 9: 387-394 [Abstract] [Full Text]
Feng, Y., Davis, N. G. (2000). Feedback Phosphorylation of the Yeast a-Factor Receptor Requires Activation of the Downstream Signaling Pathway from G Protein through Mitogen-Activated Protein Kinase. Mol. Cell. Biol. 20: 563-574 [Abstract] [Full Text]
Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. J. Cell Sci. 113: 571-585 [Abstract]
Tang, H.-Y., Xu, J., Cai, M. (2000). Pan1p, End3p, and Sla1p, Three Yeast Proteins Required for Normal Cortical Actin Cytoskeleton Organization, Associate with Each Other and Play Essential Roles in Cell Wall Morphogenesis. Mol. Cell. Biol. 20: 12-25 [Abstract] [Full Text]
Lucero, P., Peñalver, E., Vela, L., Lagunas, R. (2000). Monoubiquitination Is Sufficient To Signal Internalization of the Maltose Transporter in Saccharomyces cerevisiae. J. Bacteriol. 182: 241-243 [Abstract] [Full Text]
Lewis, M. J., Nichols, B. J., Prescianotto-Baschong, C., Riezman, H., Pelham, H. R. B. (2000). Specific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes. Mol. Biol. Cell 11: 23-38 [Abstract] [Full Text]
Engqvist-Goldstein, A. E.Y., Kessels, M. M., Chopra, V. S., Hayden, M. R., Drubin, D. G. (1999). An Actin-Binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-Coated Pits and Vesicles. JCB 147: 1503-1518 [Abstract] [Full Text]
Plant, P. J., Manolson, M. F., Grinstein, S., Demaurex, N. (1999). Alternative Mechanisms of Vacuolar Acidification in H+-ATPase-deficient Yeast. J. Biol. Chem. 274: 37270-37279 [Abstract] [Full Text]
Spelbrink, R. G., Nothwehr, S. F. (1999). The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton. Mol. Biol. Cell 10: 4263-4281 [Abstract] [Full Text]
Munn, A. L., Heese-Peck, A., Stevenson, B. J., Pichler, H., Riezman, H. (1999). Specific Sterols Required for the Internalization Step of Endocytosis in Yeast. Mol. Biol. Cell 10: 3943-3957 [Abstract] [Full Text]
Madania, A., Dumoulin, P., Grava, S., Kitamoto, H., Schärer-Brodbeck, C., Soulard, A., Moreau, V., Winsor, B. (1999). The Saccharomyces cerevisiae Homologue of Human Wiskott-Aldrich Syndrome Protein Las17p Interacts with the Arp2/3 Complex. Mol. Biol. Cell 10: 3521-3538 [Abstract] [Full Text]
Beck, T., Schmidt, A., Hall, M. N. (1999). Starvation Induces Vacuolar Targeting and Degradation of the Tryptophan Permease in Yeast. JCB 146: 1227-1238 [Abstract] [Full Text]
Zuk, D., Belk, J. P., Jacobson, A. (1999). Temperature-Sensitive Mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 Genes Result in Defects in mRNA Turnover. Genetics 153: 35-47 [Abstract] [Full Text]
Horst, M., Knecht, E. C., Schu, P. V. (1999). Import into and Degradation of Cytosolic Proteins by Isolated Yeast Vacuoles. Mol. Biol. Cell 10: 2879-2889 [Abstract] [Full Text]
Swaminathan, S., Amerik, A. Y., Hochstrasser, M. (1999). The Doa4 Deubiquitinating Enzyme Is Required for Ubiquitin Homeostasis in Yeast. Mol. Biol. Cell 10: 2583-2594 [Abstract] [Full Text]
Vida, T., Gerhardt, B. (1999). A Cell-Free Assay Allows Reconstitution of Vps33p-Dependent Transport to the Yeast Vacuole/Lysosome. JCB 146: 85-98 [Abstract] [Full Text]
Colwill, K., Field, D., Moore, L., Friesen, J., Andrews, B. (1999). In Vivo Analysis of the Domains of Yeast Rvs167p Suggests Rvs167p Function Is Mediated Through Multiple Protein Interactions. Genetics 152: 881-893 [Abstract] [Full Text]
Yang, S., Cope, M. J. T. V., Drubin, D. G. (1999). Sla2p Is Associated with the Yeast Cortical Actin Cytoskeleton via Redundant Localization Signals. Mol. Biol. Cell 10: 2265-2283 [Abstract] [Full Text]
Nierras, C. R., Warner, J. R. (1999). Protein Kinase C Enables the Regulatory Circuit That Connects Membrane Synthesis to Ribosome Synthesis in Saccharomyces cerevisiae. J. Biol. Chem. 274: 13235-13241 [Abstract] [Full Text]
Peñalver, E., Lucero, P., Moreno, E., Lagunas, R. (1999). Clathrin and Two Components of the COPII Complex, Sec23p and Sec24p, Could Be Involved in Endocytosis of the Saccharomyces cerevisiae Maltose Transporter. J. Bacteriol. 181: 2555-2563 [Abstract] [Full Text]
Ayscough, K. R., Eby, J. J., Lila, T., Dewar, H., Kozminski, K. G., Drubin, D. G. (1999). Sla1p Is a Functionally Modular Component of the Yeast Cortical Actin Cytoskeleton Required for Correct Localization of Both Rho1p-GTPase and Sla2p, a Protein with Talin Homology. Mol. Biol. Cell 10: 1061-1075 [Abstract] [Full Text]
Cope, M.J. T.V., Yang, S., Shang, C., Drubin, D. G. (1999). Novel Protein Kinases Ark1p and Prk1p Associate with and Regulate the Cortical Actin Cytoskeleton in Budding Yeast. JCB 144: 1203-1218 [Abstract] [Full Text]
Mulholland, J., Konopka, J., Singer-Kruger, B., Zerial, M., Botstein, D. (1999). Visualization of Receptor-mediated Endocytosis in Yeast. Mol. Biol. Cell 10: 799-817 [Abstract] [Full Text]
Liu, X. F., Culotta, V. C. (1999). Post-translation Control of Nramp Metal Transport in Yeast. ROLE OF METAL IONS AND THE BSD2 GENE. J. Biol. Chem. 274: 4863-4868 [Abstract] [Full Text]
Medintz, I., Jiang, H., Michels, C. A. (1998). The Role of Ubiquitin Conjugation in Glucose-induced Proteolysis of Saccharomyces Maltose Permease. J. Biol. Chem. 273: 34454-34462 [Abstract] [Full Text]
Holthuis, J. C.�M., Nichols, B. J., Pelham, H. R.�B. (1998). The Syntaxin Tlg1p Mediates Trafficking of Chitin Synthase III to Polarized Growth Sites in Yeast. Mol. Biol. Cell 9: 3383-3397 [Abstract] [Full Text]
Gitan, R. S., Luo, H., Rodgers, J., Broderius, M., Eide, D. (1998). Zinc-induced Inactivation of the Yeast ZRT1 Zinc Transporter Occurs through Endocytosis and Vacuolar Degradation. J. Biol. Chem. 273: 28617-28624 [Abstract] [Full Text]
Otsuga, D., Keegan, B. R., Brisch, E., Thatcher, J. W., Hermann, G. J., Bleazard, W., Shaw, J. M. (1998). The Dynamin-related GTPase, Dnm1p, Controls Mitochondrial Morphology in Yeast. JCB 143: 333-349 [Abstract] [Full Text]
Dosil, M., Giot, L., Davis, C., Konopka, J. B. (1998). Dominant-Negative Mutations in the G-Protein-Coupled alpha -Factor Receptor Map to the Extracellular Ends of the Transmembrane Segments. Mol. Cell. Biol. 18: 5981-5991 [Abstract] [Full Text]
Maldonado, A. M., de la Fuente, N., Portillo, F. (1998). Characterization of an Allele-Nonspecific Intragenic Suppressor in the Yeast Plasma Membrane H+-ATPase Gene (PMA1). Genetics 150: 11-19 [Abstract] [Full Text]
Roth, A. F., Sullivan, D. M., Davis, N. G. (1998). A Large PEST-like Sequence Directs the Ubiquitination, Endocytosis, and Vacuolar Degradation of the Yeast a-Factor Receptor. JCB 142: 949-961 [Abstract] [Full Text]
Brondijk, T. H. C., van der Rest, M. E., Pluim, D., de Vries, Y., Stingl, K., Poolman, B., Konings, W. N. (1998). Catabolite Inactivation of Wild-type and Mutant Maltose Transport Proteins in Saccharomyces cerevisiae. J. Biol. Chem. 273: 15352-15357 [Abstract] [Full Text]
Ziman, M., Chuang, J. S., Tsung, M., Hamamoto, S., Schekman, R. (1998). Chs6p-dependent Anterograde Transport of Chs3p from the Chitosome to the Plasma Membrane in Saccharomyces cerevisiae. Mol. Biol. Cell 9: 1565-1576 [Abstract] [Full Text]
Brizzio, V., Gammie, A. E., Rose, M. D. (1998). Rvs161p Interacts with Fus2p to Promote Cell Fusion in Saccharomyces cerevisiae. JCB 141: 567-584 [Abstract] [Full Text]
Wendland, B., Emr, S. D. (1998). Pan1p, Yeast eps15, Functions as a Multivalent Adaptor That Coordinates Protein-Protein Interactions Essential for Endocytosis. JCB 141: 71-84 [Abstract] [Full Text]
Chiang, M.-C., Chiang, H.-L. (1998). Vid24p, a Novel Protein Localized to the Fructose-1,6-bisphosphatase-containing Vesicles, Regulates Targeting of Fructose-1,6-bisphosphatase from the Vesicles to the Vacuole for Degradation. JCB 140: 1347-1356 [Abstract] [Full Text]
Bryant, N. J., Stevens, T. H. (1998). Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole. Microbiol. Mol. Biol. Rev. 62: 230-247 [Abstract] [Full Text]
Shieh, H.-L., Chiang, H.-L. (1998). In Vitro Reconstitution of Glucose-induced Targeting of Fructose-1,6-bisphosphatase into the Vacuole in Semi-intact Yeast Cells. J. Biol. Chem. 273: 3381-3387 [Abstract] [Full Text]