A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen

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A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen

MS Diamond and TA Springer
Committee on Cell and Developmental Biology, Harvard Medical School, Boston, Massachusetts.

We report that a subpopulation (10%) of the Mac-1 (CD1 1b/CD18) molecules on activated neutrophils mediates adhesion to ICAM-1 and fibrinogen. We describe a novel mAb (CBRM1/5) that binds to an activation-specific neoepitope on a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorobol esters but does not recognize Mac-1 on resting myeloid cells. CBRM1/5 immunoprecipitates a subpopulation of Mac-1 molecules from detergent lysates of neutrophils, binds to immunoaffinity-purified Mac- 1, and localizes to the I domain on the alpha chain of Mac-1. Because CBRM1/5 recognizes a fraction of Mac-1 on activated neutrophils, but still blocks Mac-1-dependent adhesion to fibrinogen and ICAM-1, we suggest that only a small subset of Mac-1 molecules is competent to mediate adhesion.
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Semmrich, M., Smith, A., Feterowski, C., Beer, S., Engelhardt, B., Busch, D. H., Bartsch, B., Laschinger, M., Hogg, N., Pfeffer, K., Holzmann, B. (2005). Importance of integrin LFA-1 deactivation for the generation of immune responses. JEM 201: 1987-1998 [Abstract] [Full Text]
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Ma, Y.-Q., Plow, E. F., Geng, J.-G. (2004). P-selectin binding to P-selectin glycoprotein ligand-1 induces an intermediate state of {alpha}M{beta}2 activation and acts cooperatively with extracellular stimuli to support maximal adhesion of human neutrophils. Blood 104: 2549-2556 [Abstract] [Full Text]
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Sachs, U. J. H., Chavakis, T., Fung, L., Lohrenz, A., Bux, J., Reil, A., Ruf, A., Santoso, S. (2004). Human alloantibody anti-Mart interferes with Mac-1-dependent leukocyte adhesion. Blood 104: 727-734 [Abstract] [Full Text]
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Schoolmeester, A., Vanhoorelbeke, K., Katsutani, S., Depraetere, H., Feys, H. B., Heemskerk, J. M. W., Hoylaerts, M. F., Deckmyn, H. (2004). Monoclonal antibody IAC-1 is specific for activated {alpha}2{beta}1 and binds to amino acids 199 to 201 of the integrin {alpha}2 I-domain. Blood 104: 390-396 [Abstract] [Full Text]
Pluskota, E., Soloviev, D. A., Bdeir, K., Cines, D. B., Plow, E. F. (2004). Integrin {alpha}M{beta}2 Orchestrates and Accelerates Plasminogen Activation and Fibrinolysis by Neutrophils. J. Biol. Chem. 279: 18063-18072 [Abstract] [Full Text]
Wetzel, A., Chavakis, T., Preissner, K. T., Sticherling, M., Haustein, U.-F., Anderegg, U., Saalbach, A. (2004). Human Thy-1 (CD90) on Activated Endothelial Cells Is a Counterreceptor for the Leukocyte Integrin Mac-1 (CD11b/CD18). J. Immunol. 172: 3850-3859 [Abstract] [Full Text]
Hixson, P., Smith, C. W., Shurin, S. B., Tosi, M. F. (2004). Unique CD18 mutations involving a deletion in the extracellular stalk region and a major truncation of the cytoplasmic domain in a patient with leukocyte adhesion deficiency type 1. Blood 103: 1105-1113 [Abstract] [Full Text]
Ehlers, R., Ustinov, V., Chen, Z., Zhang, X., Rao, R., Luscinskas, F. W., Lopez, J., Plow, E., Simon, D. I. (2003). Targeting Platelet-Leukocyte Interactions: Identification of the Integrin Mac-1 Binding Site for the Platelet Counter Receptor Glycoprotein Ib{alpha}. JEM 198: 1077-1088 [Abstract] [Full Text]
El-Azami-El-Idrissi, M., Bauche, C., Loucka, J., Osicka, R., Sebo, P., Ladant, D., Leclerc, C. (2003). Interaction of Bordetella pertussis Adenylate Cyclase with CD11b/CD18: ROLE OF TOXIN ACYLATION AND IDENTIFICATION OF THE MAIN INTEGRIN INTERACTION DOMAIN. J. Biol. Chem. 278: 38514-38521 [Abstract] [Full Text]
Willeke, T., Schymeinsky, J., Prange, P., Zahler, S., Walzog, B. (2003). A role for Syk-kinase in the control of the binding cycle of the {beta}2 integrins (CD11/CD18) in human polymorphonuclear neutrophils. J. Leukoc. Biol. 74: 260-269 [Abstract] [Full Text]
Liu, L., Hakansson, L., Ridefelt, P., Garcia, R. C., Venge, P. (2003). Priming of Eosinophil Migration Across Lung Epithelial Cell Monolayers and Upregulation of CD11b/CD18 Are Elicited by Extracellular Ca2+. Am. J. Respir. Cell Mol. Bio. 28: 713-721 [Abstract] [Full Text]
Alon, R., Aker, M., Feigelson, S., Sokolovsky-Eisenberg, M., Staunton, D. E., Cinamon, G., Grabovsky, V., Shamri, R., Etzioni, A. (2003). A novel genetic leukocyte adhesion deficiency in subsecond triggering of integrin avidity by endothelial chemokines results in impaired leukocyte arrest on vascular endothelium under shear flow. Blood 101: 4437-4445 [Abstract] [Full Text]
Ghiran, I., Klickstein, L. B., Nicholson-Weller, A. (2003). Calreticulin Is at the Surface of Circulating Neutrophils and Uses CD59 as an Adaptor Molecule. J. Biol. Chem. 278: 21024-21031 [Abstract] [Full Text]
May, A. E., Redecke, V., Gruner, S., Schmidt, R., Massberg, S., Miethke, T., Ryba, B., Prazeres da Costa, C., Schomig, A., Neumann, F.-J. (2003). Recruitment of Chlamydia pneumoniae-Infected Macrophages to the Carotid Artery Wall in Noninfected, Nonatherosclerotic Mice. Arterioscler. Thromb. Vasc. Bio. 23: 789-794 [Abstract] [Full Text]
Pluskota, E., Soloviev, D. A., Plow, E. F. (2003). Convergence of the adhesive and fibrinolytic systems: recognition of urokinase by integrin alpha Mbeta 2 as well as by the urokinase receptor regulates cell adhesion and migration. Blood 101: 1582-1590 [Abstract] [Full Text]
Shimaoka, M., Lu, C., Salas, A., Xiao, T., Takagi, J., Springer, T. A. (2002). Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds. Proc. Natl. Acad. Sci. USA 99: 16737-16741 [Abstract] [Full Text]
Xia, Y., Borland, G., Huang, J., Mizukami, I. F., Petty, H. R., Todd, R. F. III, Ross, G. D. (2002). Function of the Lectin Domain of Mac-1/Complement Receptor Type 3 (CD11b/CD18) in Regulating Neutrophil Adhesion. J. Immunol. 169: 6417-6426 [Abstract] [Full Text]
May, A. E., Schmidt, R., Kanse, S. M., Chavakis, T., Stephens, R. W., Schomig, A., Preissner, K. T., Neumann, F.-J. (2002). Urokinase receptor surface expression regulates monocyte adhesion in acute myocardial infarction. Blood 100: 3611-3617 [Abstract] [Full Text]
Nupponen, I., Pesonen, E., Andersson, S., Makela, A., Turunen, R., Kautiainen, H., Repo, H. (2002). Neutrophil Activation in Preterm Infants Who Have Respiratory Distress Syndrome. Pediatrics 110: 36-41 [Abstract] [Full Text]
Jones, S. L. (2002). Protein kinase A regulates {beta}2 integrin avidity in neutrophils. J. Leukoc. Biol. 71: 1042-1048 [Abstract] [Full Text]
Schober, J. M., Chen, N., Grzeszkiewicz, T. M., Jovanovic, I., Emeson, E. E., Ugarova, T. P., Ye, R. D., Lau, L. F., Lam, S. C.-T. (2002). Identification of integrin alpha Mbeta 2 as an adhesion receptor on peripheral blood monocytes for Cyr61 (CCN1) and connective tissue growth factor (CCN2): immediate-early gene products expressed in atherosclerotic lesions. Blood 99: 4457-4465 [Abstract] [Full Text]
Berger, M., Budhu, S., Lu, E., Li, Y., Loike, D., Silverstein, S. C., Loike, J. D. (2002). Different Gi-coupled chemoattractant receptors signal qualitatively different functions in human neutrophils. J. Leukoc. Biol. 71: 798-806 [Abstract] [Full Text]
Dominguez-Jimenez, C., Sancho, D., Nieto, M., Montoya, M. C., Barreiro, O., Sanchez-Madrid, F., Gonzalez-Amaro, R. (2002). Effect of pentoxifylline on polarization and migration of human leukocytes. J. Leukoc. Biol. 71: 588-596 [Abstract] [Full Text]
Yoshitake, H., Takeda, Y., Nitto, T., Sendo, F. (2002). Cross-linking of GPI-80, a possible regulatory molecule of cell adhesion, induces up-regulation of CD11b/CD18 expression on neutrophil surfaces and shedding of L-selectin. J. Leukoc. Biol. 71: 205-211 [Abstract] [Full Text]
Szaba, F. M., Smiley, S. T. (2002). Roles for thrombin and fibrin(ogen) in cytokine/chemokine production and macrophage adhesion in vivo. Blood 99: 1053-1059 [Abstract] [Full Text]
Beals, C. R., Edwards, A. C., Gottschalk, R. J., Kuijpers, T. W., Staunton, D. E. (2001). CD18 Activation Epitopes Induced by Leukocyte Activation. J. Immunol. 167: 6113-6122 [Abstract] [Full Text]
Bechard, D., Scherpereel, A., Hammad, H., Gentina, T., Tsicopoulos, A., Aumercier, M., Pestel, J., Dessaint, J.-P., Tonnel, A.-B., Lassalle, P. (2001). Human Endothelial-Cell Specific Molecule-1 Binds Directly to the Integrin CD11a/CD18 (LFA-1) and Blocks Binding to Intercellular Adhesion Molecule-1. J. Immunol. 167: 3099-3106 [Abstract] [Full Text]
Smiley, S. T., King, J. A., Hancock, W. W. (2001). Fibrinogen Stimulates Macrophage Chemokine Secretion Through Toll-Like Receptor 4. J. Immunol. 167: 2887-2894 [Abstract] [Full Text]
Thiel, M., Buessecker, F., Eberhardt, K., Chouker, A., Setzer, F., Kreimeier, U., Arfors, K.-E., Peter, K., Messmer, K. (2001). Effects of hypertonic saline on expression of human polymorphonuclear leukocyte adhesion molecules. J. Leukoc. Biol. 70: 261-273 [Abstract] [Full Text]
Woska, J. R. Jr., Shih, D.-t., Taqueti, V. R., Hogg, N., Kelly, T. A., Kishimoto, T. K. (2001). A small-molecule antagonist of LFA-1 blocks a conformational change important for LFA-1 function. J. Leukoc. Biol. 70: 329-334 [Abstract] [Full Text]
Hafezi-Moghadam, A., Thomas, K. L., Prorock, A. J., Huo, Y., Ley, K. (2001). L-Selectin Shedding Regulates Leukocyte Recruitment. JEM 193: 863-872 [Abstract] [Full Text]
van den Berg, J. M., Weyer, S., Weening, J. J., Roos, D., Kuijpers, T. W. (2001). Divergent effects of tumor necrosis factor {alpha} on apoptosis of human neutrophils. J. Leukoc. Biol. 69: 467-473 [Abstract] [Full Text]
Lipton, B. P., Bautista, A. P., Delcarpio, J. B., McDonough, K. H. (2001). Effects of endotoxin on neutrophil-mediated I/R injury in isolated perfused rat hearts. Am. J. Physiol. Heart Circ. Physiol. 280: H802-H811 [Abstract] [Full Text]
Cox, D., Dale, B. M., Kashiwada, M., Helgason, C. D., Greenberg, S. (2001). A Regulatory Role for Src Homology 2 Domain-Containing Inositol 5'-Phosphatase (Ship) in Phagocytosis Mediated by Fc{gamma} Receptors and Complement Receptor 3 ({alpha}M{beta}2; Cd11b/Cd18). JEM 193: 61-72 [Abstract] [Full Text]
Joussen, A. M., Murata, T., Tsujikawa, A., Kirchhof, B., Bursell, S.-E., Adamis, A. P. (2001). Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina. Am. J. Pathol. 158: 147-152 [Abstract] [Full Text]
Whitlock, B. B., Gardai, S., Fadok, V., Bratton, D., Henson, P. M. (2000). Differential Roles for {alpha}M{beta}2 Integrin Clustering or Activation in the Control of Apoptosis via Regulation of Akt and ERK Survival Mechanisms. JCB 151: 1305-1320 [Abstract] [Full Text]
Lindner, J. R., Kahn, M. L., Coughlin, S. R., Sambrano, G. R., Schauble, E., Bernstein, D., Foy, D., Hafezi-Moghadam, A., Ley, K. (2000). Delayed Onset of Inflammation in Protease-Activated Receptor-2-Deficient Mice. J. Immunol. 165: 6504-6510 [Abstract] [Full Text]
Orchekowski, R. P., Plescia, J., Altieri, D. C., Bajt, M. L. (2000). {alpha}M{beta}2 (CD11b/CD18, Mac-1) integrin activation by a unique monoclonal antibody to {alpha}M I domain that is divalent cation-sensitive. J. Leukoc. Biol. 68: 641-649 [Abstract] [Full Text]
Greenstein, S., Barnard, J., Zhou, K., Fong, M., Hendey, B. (2000). Fas activation reduces neutrophil adhesion to endothelial cells. J. Leukoc. Biol. 68: 715-722 [Abstract] [Full Text]
Menegazzi, R., Busetto, S., Cramer, R., Dri, P., Patriarca, P. (2000). Role of Intracellular Chloride in the Reversible Activation of Neutrophil {beta}2 Integrins: A Lesson from TNF Stimulation. J. Immunol. 165: 4606-4614 [Abstract] [Full Text]
Kupatt, C., Habazettl, H., Hanusch, P., Wichels, R., Hahnel, D., Becker, B. F., Boekstegers, P. (2000). c7E3Fab Reduces Postischemic Leukocyte-Thrombocyte Interaction Mediated by Fibrinogen : Implications for Myocardial Reperfusion Injury. Arterioscler. Thromb. Vasc. Bio. 20: 2226-2232 [Abstract] [Full Text]
Wagner, J. G., Roth, R. A. (2000). Neutrophil Migration Mechanisms, with an Emphasis on the Pulmonary Vasculature. Pharmacol. Rev. 52: 349-374 [Abstract] [Full Text]
Lindner, J. R., Dayton, P. A., Coggins, M. P., Ley, K., Song, J., Ferrara, K., Kaul, S. (2000). Noninvasive Imaging of Inflammation by Ultrasound Detection of Phagocytosed Microbubbles. Circulation 102: 531-538 [Abstract] [Full Text]
May, A. E., Neumann, F.-J., Schomig, A., Preissner, K. T. (2000). VLA-4 (alpha 4beta 1) engagement defines a novel activation pathway for beta 2 integrin-dependent leukocyte adhesion involving the urokinase receptor. Blood 96: 506-513 [Abstract] [Full Text]
Salas, A, Sans, M, Soriano, A, Reverter, J C, Anderson, D C, Pique, J M, Panes, J (2000). Heparin attenuates TNF-alpha induced inflammatory response through a CD11b dependent mechanism. Gut 47: 88-96 [Abstract] [Full Text]
Huth, J. R., Olejniczak, E. T., Mendoza, R., Liang, H., Harris, E. A. S., Lupher, M. L. Jr., Wilson, A. E., Fesik, S. W., Staunton, D. E. (2000). NMR and mutagenesis evidence for an I domain allosteric site that regulates lymphocyte function-associated antigen 1 ligand binding. Proc. Natl. Acad. Sci. USA 97: 5231-5236 [Abstract] [Full Text]
Pierini, L. M., Lawson, M. A., Eddy, R. J., Hendey, B., Maxfield, F. R. (2000). Oriented endocytic recycling of alpha 5beta 1 in motile neutrophils. Blood 95: 2471-2480 [Abstract] [Full Text]
Barouch, F. C., Miyamoto, K., Allport, J. R., Fujita, K., Bursell, S.-E., Aiello, L. P., Luscinskas, F. W., Adamis, A. P. (2000). Integrin-Mediated Neutrophil Adhesion and Retinal Leukostasis in Diabetes. IOVS 41: 1153-1158 [Abstract] [Full Text]
Lindner, J. R., Coggins, M. P., Kaul, S., Klibanov, A. L., Brandenburger, G. H., Ley, K. (2000). Microbubble Persistence in the Microcirculation During Ischemia/Reperfusion and Inflammation Is Caused by Integrin- and Complement-Mediated Adherence to Activated Leukocytes. Circulation 101: 668-675 [Abstract] [Full Text]
De Caterina, R., Liao, J. K, Libby, P. (2000). Fatty acid modulation of endothelial activation. Am. J. Clin. Nutr. 71: 213S-223S [Abstract] [Full Text]
Lo, S. K., Rahman, A., Xu, N., Zhou, M. Y., Nagpala, P., Jaffe, H. A., Malik, A. B. (1999). Neutrophil Inhibitory Factor Abrogates Neutrophil Adhesion by Blockade of CD11a and CD11b beta 2 Integrins. Mol. Pharmacol. 56: 926-932 [Abstract] [Full Text]
Hoffer, E, Baum, Y, Tabak, A, Frevert, C (1999). Adhesion molecules of blood polymorphonuclear leukocytes and alveolar macrophages in rats: modulation by exposure to ozone. Hum Exp Toxicol 18: 547-551 [Abstract]
Ottonello, L., Epstein, A.L., Dapino, P., Barbera, P., Morone, P., Dallegri, F. (1999). Monoclonal Lym-1 Antibody-Dependent Cytolysis by Neutrophils Exposed to Granulocyte-Macrophage Colony-Stimulating Factor: Intervention of Fc{gamma}RII (CD32), CD11b-CD18 Integrins, and CD66b Glycoproteins. Blood 93: 3505-3511 [Abstract] [Full Text]
Perez, R. L., Ritzenthaler, J. D., Roman, J. (1999). Transcriptional Regulation of the Interleukin-1beta Promoter via Fibrinogen Engagement of the CD18 Integrin Receptor. Am. J. Respir. Cell Mol. Bio. 20: 1059-1066 [Abstract] [Full Text]
Weber, K. S.C., Klickstein, L. B., Weber, C. (1999). Specific Activation of Leukocyte beta 2 Integrins Lymphocyte Function-associated Antigen-1 and Mac-1 by Chemokines Mediated by Distinct Pathways via the alpha �Subunit Cytoplasmic Domains. Mol. Biol. Cell 10: 861-873 [Abstract] [Full Text]
Oxvig, C., Lu, C., Springer, T. A. (1999). Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc. Natl. Acad. Sci. USA 96: 2215-2220 [Abstract] [Full Text]
Mazzucchelli, L., Burritt, J. B., Jesaitis, A. J., Nusrat, A., Liang, T. W., Gewirtz, A. T., Schnell, F. J., Parkos, C. A. (1999). Cell-Specific Peptide Binding by Human Neutrophils. Blood 93: 1738-1748 [Abstract] [Full Text]
Vuorte, J., Lindsberg, P. J., Kaste, M., Meri, S., Jansson, S.-E., Rothlein, R., Repo, H. (1999). Anti-ICAM-1 Monoclonal Antibody R6.5 (Enlimomab) Promotes Activation of Neutrophils in Whole Blood. J. Immunol. 162: 2353-2357 [Abstract] [Full Text]
Chen, C., Mobley, J. L., Dwir, O., Shimron, F., Grabovsky, V., Lobb, R. R., Shimizu, Y., Alon, R. (1999). High Affinity Very Late Antigen-4 Subsets Expressed on T Cells Are Mandatory for Spontaneous Adhesion Strengthening But Not for Rolling on VCAM-1 in Shear Flow. J. Immunol. 162: 1084-1095 [Abstract] [Full Text]
Soriano, S. G., Coxon, A., Wang, Y. F., Frosch, M. P., Lipton, S. A., Hickey, P. R., Mayadas, T. N., Chan, P. H. (1999). Mice Deficient in Mac-1 (CD11b/CD18) Are Less Susceptible to Cerebral Ischemia/Reperfusion Injury � Editorial Comment. Stroke 30: 134-139 [Abstract] [Full Text]
Li, R., Rieu, P., Griffith, D. L., Scott, D., Amin Arnaout, M. (1998). Two Functional States of the CD11b A-Domain: Correlations with Key Features of Two Mn2+-complexed Crystal Structures. JCB 143: 1523-1534 [Abstract] [Full Text]
Asimakopoulos, G., Taylor, K. M. (1998). Effects of cardiopulmonary bypass on leukocyte and endothelial adhesion molecules. Ann. Thorac. Surg. 66: 2135-2144 [Abstract] [Full Text]