BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans

doi:10.1083/jcb.120.4.959

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BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans

PM Mirabito and NR Morris
Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.

The Aspergillus nidulans bimA gene is required for mitosis. Loss of function mutations in bimA cause cells to arrest growth with condensed chromatin and a short, metaphaselike mitotic spindle. bimA is a member of a gene family defined by a repeated motif called the Tetratrico Peptide Repeat (TPR), which is found in genes from bacteria, yeast and insects. Several yeast TPR genes are also required for mitosis, including Saccharomyces cerevisiae CDC27 and Schizosaccharomyces pombe nuc2+, which appear to be functional homologs of bimA. We have developed antisera specific to the bimA protein (BIMA) and have characterized BIMA by western blot and immunocytochemical analyses. BIMA is heterogeneous in apparent molecular weight, consisting of a major 90-kD species and at least two minor species of approximately 105 kD. The results of BIMA localization by immunofluorescence microscopy depend on the level of BIMA expression. Overexpression of BIMA, which had no deleterious affect on growth or mitosis, resulted in localization of BIMA on or throughout most nuclei. Nuclear staining was granular, and overlapped but was not completely coincident with DNA staining by DAPI. In contrast, when expressed at normal levels, BIMA colocalized with the spindle pole body (SPB). BIMA localized to the SPB in a cell cycle independent manner. These results show that BIMA is either associated with or is a component of the SPB, and they suggest that BIMA functions at the spindle poles to promote the onset of anaphase.
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Ye, X. S., Fincher, R. R., Tang, A., Osmani, A. H., Osmani, S. A. (1998). Regulation of the Anaphase-promoting Complex/Cyclosome by bimAAPC3 and Proteolysis of NIMA. Mol. Biol. Cell 9: 3019-3030 [Abstract] [Full Text]
Osherov, N., Yamashita, R. A., Chung, Y.-S., May, G. S. (1998). Structural Requirements for in Vivo Myosin I Function in Aspergillus nidulans. J. Biol. Chem. 273: 27017-27025 [Abstract] [Full Text]
Wu, L., Osmani, S.A., Mirabito, P.M. (1998). A Role for NIMA in the Nuclear Localization of Cyclin B in Aspergillus nidulans. JCB 141: 1575-1587 [Abstract] [Full Text]
Hagan, I. (1998). The fission yeast microtubule cytoskeleton. J. Cell Sci. 111: 1603-1612 [Abstract]
Lies, C., Cheng, J, James, S., Morris, N., O'Connell, M., Mirabito, P. (1998). BIMAAPC3, a component of the Aspergillus anaphase promoting complex/cyclosome, is required for a G2 checkpoint blocking entry into mitosis in the absence of NIMA function. J. Cell Sci. 111: 1453-1465 [Abstract]
Jörgensen, P.-M., Brundell, E., Starborg, M., Höög, C. (1998). A Subunit of the Anaphase-Promoting Complex Is a Centromere-Associated Protein in Mammalian Cells. Mol. Cell. Biol. 18: 468-476 [Abstract] [Full Text]
Rieder, C. L., Khodjakov, A., Paliulis, L. V., Fortier, T. M., Cole, R. W., Sluder, G. (1997). Mitosis in vertebrate somatic cells with two spindles: Implications for the metaphase/anaphase transition checkpoint�and�cleavage. Proc. Natl. Acad. Sci. USA 94: 5107-5112 [Abstract] [Full Text]
King, R. W., Deshaies, R. J., Peters, J.-M., Kirschner, M. W. (1996). How Proteolysis Drives the Cell Cycle. Science 274: 1652-1659 [Abstract] [Full Text]
Nabeshima, K, Kurooka, H, Takeuchi, M, Kinoshita, K, Nakaseko, Y, Yanagida, M (1995). p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.. Genes Dev. 9: 1572-1585 [Abstract]
James, S., Mirabito, P., Scacheri, P., Morris, N. (1995). The Aspergillus nidulans bimE (blocked-in-mitosis) gene encodes multiple cell cycle functions involved in mitotic checkpoint control and mitosis. J. Cell Sci. 108: 3485-3499 [Abstract]
Kumada, K, Su, S, Yanagida, M, Toda, T (1995). Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation. J. Cell Sci. 108: 895-905 [Abstract]