Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180: a modular structure similar to tenascin

doi:10.1083/jcb.120.5.1237

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Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180: a modular structure similar to tenascin

B Fuss, ES Wintergerst, U Bartsch and M Schachner
Department of Neurobiology, Swiss Federal Institute of Technology, Zurich.

The oligodendrocyte-derived extracellular matrix glycoprotein J1- 160/180 is a recognition molecule expressed exclusively in the central nervous system. J1-160/180 has been shown to be adhesive for astrocytes and repellent towards neurons and growth cones. We report here the complete nucleotide sequence of J1-160/180 in the rat. The predicted amino acid sequence showed a structural architecture very similar to tenascin: a cysteine-rich amino terminal region is followed by 4.5 epidermal growth factor-like repeats, 9 fibronectin type III homologous repeats and a domain homologous to fibrinogen. Sequence comparison analysis revealed highest homology of rat J1-160/180 to mouse tenascin and chicken restrictin with a similarity of 66% and 85%, respectively. The J1-160/180-coding mRNA is derived from a single copy gene. Using the polymerase chain reaction we could show that two J1-160/180 isoforms are generated by alternative splicing of the sixth fibronectin type III homologous repeat. Localization of J1-160/180 mRNA by in situ hybridization in the cerebellum, hippocampus and olfactory bulb confirmed the expression of J1-160/180 by oligodendrocytes with a peak of transcription at 7-14 d after birth, indicating a functional role during myelination. In addition, J1-160/180-specific RNA was found in a small subset of neurons in all three structures of the CNS analyzed. These neurons continue to express J1-160/180 in the adult.
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Xiao, Z.-C., Ragsdale, D. S., Malhotra, J. D., Mattei, L. N., Braun, P. E., Schachner, M., Isom, L. L. (1999). Tenascin-R Is a Functional Modulator of Sodium Channel beta Subunits. J. Biol. Chem. 274: 26511-26517 [Abstract] [Full Text]
Zacharias, U., Norenberg, U., Rathjen, F. G. (1999). Functional Interactions of the Immunoglobulin Superfamily Member F11 Are Differentially Regulated by the Extracellular Matrix Proteins Tenascin-R and Tenascin-C. J. Biol. Chem. 274: 24357-24365 [Abstract] [Full Text]
Weber, P., Bartsch, U., Rasband, M. N., Czaniera, R., Lang, Y., Bluethmann, H., Margolis, R. U., Levinson, S. R., Shrager, P., Montag, D., Schachner, M. (1999). Mice Deficient for Tenascin-R Display Alterations of the Extracellular Matrix and Decreased Axonal Conduction Velocities in the CNS. J. Neurosci. 19: 4245-4262 [Abstract] [Full Text]
Becker, C. G., Becker, T., Meyer, R. L., Schachner, M. (1999). Tenascin-R Inhibits the Growth of Optic Fibers In Vitro But Is Rapidly Eliminated during Nerve Regeneration in the Salamander Pleurodeles waltl. J. Neurosci. 19: 813-827 [Abstract] [Full Text]
David, C. L., Orpiszewski, J., Zhu, X.-C., Reissner, K. J., Aswad, D. W. (1998). Isoaspartate in Chrondroitin Sulfate Proteoglycans of Mammalian Brain. J. Biol. Chem. 273: 32063-32070 [Abstract] [Full Text]
Angelov, D. N., Walther, M., Streppel, M., Guntinas-Lichius, O., Neiss, W. F., Probstmeier, R., Pesheva, P. (1998). Tenascin-R Is Antiadhesive for Activated Microglia that Induce Downregulation of the Protein after Peripheral Nerve Injury: a New Role in Neuronal Protection. J. Neurosci. 18: 6218-6229 [Abstract] [Full Text]
Waschek, J. A., Casillas, R. A., Nguyen, T. B., DiCicco-Bloom, E. M., Carpenter, E. M., Rodriguez, W. I. (1998). Neural tube expression of pituitary adenylate cyclase-activating peptide (PACAP) and receptor: Potential role in patterning and neurogenesis. Proc. Natl. Acad. Sci. USA 95: 9602-9607 [Abstract] [Full Text]
Kammerer, R. A., Schulthess, T., Landwehr, R., Lustig, A., Fischer, D., Engel, J. (1998). Tenascin-C Hexabrachion Assembly Is a Sequential Two-step Process Initiated by Coiled-coil alpha -Helices. J. Biol. Chem. 273: 10602-10608 [Abstract] [Full Text]
Dupree, J. L., Coetzee, T., Blight, A., Suzuki, K., Popko, B. (1998). Myelin Galactolipids Are Essential for Proper Node of Ranvier Formation in the CNS. J. Neurosci. 18: 1642-1649 [Abstract] [Full Text]
Luczak, J. A., Redick, S. D., Schwarzbauer, J. E. (1998). A Single Cysteine, Cys-64, Is Essential for Assembly of Tenascin-C Hexabrachions. J. Biol. Chem. 273: 2073-2077 [Abstract] [Full Text]
Xiao, Z.-C., Bartsch, U., Margolis, R. K., Rougon, G., Montag, D., Schachner, M. (1997). Isolation of a Tenascin-R Binding Protein from Mouse Brain Membranes. A PHOSPHACAN-RELATED CHONDROITIN SULFATE PROTEOGLYCAN. J. Biol. Chem. 272: 32092-32101 [Abstract] [Full Text]
Fuss, B., Baba, H., Phan, T., Tuohy, V. K., Macklin, W. B. (1997). Phosphodiesterase I, A Novel Adhesion Molecule and/or Cytokine Involved in Oligodendrocyte Function. J. Neurosci. 17: 9095-9103 [Abstract] [Full Text]
Clark, R. A., Erickson, H. P., Springer, T. A. (1997). Tenascin Supports Lymphocyte Rolling. JCB 137: 755-765 [Abstract] [Full Text]
Leprini, A., Gherzi, R., Siri, A., Querze, G., Viti, F., Zardi, L. (1996). The Human Tenascin-R Gene. J. Biol. Chem. 271: 31251-31254 [Abstract] [Full Text]
Carnemolla, B., Leprini, A., Borsi, L., Querz�, G., Urbini, S., Zardi, L. (1996). Human Tenascin-R. J. Biol. Chem. 271: 8157-8160 [Abstract] [Full Text]
Thomas, L. B., Steindler, D. A. (1995). Review : Glial Boundaries and Scars: Programs for Normal Development and Wound Healing in the Brain. Neuroscientist 1: 142-154 [Abstract]
Gherzi, R., Carnemolla, B., Siri, A., Ponassi, M., Balza, E., Zardi, L. (1995). Human Tenascin Gene. J. Biol. Chem. 270: 3429-3434 [Abstract] [Full Text]
Redick, S., Schwarzbauer, J. (1995). Rapid intracellular assembly of tenascin hexabrachions suggests a novel cotranslational process. J. Cell Sci. 108: 1761-1769 [Abstract]
Pesheva, P, Probstmeier, R, Skubitz, A., McCarthy, J., Furcht, L., Schachner, M (1994). Tenascin-R (J1 160/180 inhibits fibronectin-mediated cell adhesion--functional relatedness to tenascin-C. J. Cell Sci. 107: 2323-2333 [Abstract]
Ellis, M., Weber, U, Wiersdorff, V, Mlodzik, M (1994). Confrontation of scabrous expressing and non-expressing cells is essential for normal ommatidial spacing in the Drosophila eye. Development 120: 1959-1969 [Abstract]
Wehrle-Haller, B, Chiquet, M (1993). Dual function of tenascin: simultaneous promotion of neurite growth and inhibition of glial migration. J. Cell Sci. 106: 597-610 [Abstract]