Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina

doi:10.1083/jcb.121.2.335

This Article

	Full Text (PDF, 2744K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gundersen, D.
	Articles by Rodriguez-Boulan, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gundersen, D.
	Articles by Rodriguez-Boulan, E.


Social Bookmarking

	What's this?

ARTICLES

Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina

D Gundersen, SK Powell and E Rodriguez-Boulan
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.

The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. Strikingly, culture of RPE results in rapid redistribution of N-CAM to the basolateral surface. This is not due to an isoform shift, since the N-CAM expressed by cultured cells (140 kD) is the same as that expressed by RPE in vivo. Rather, the reversed polarity of N-CAM appears to result from the disruption of the contact between the RPE and the photoreceptors of the neural retina. We suggest that N-CAM in RPE and photoreceptors participate in these interactions.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Nandrot, E. F., Anand, M., Sircar, M., Finnemann, S. C. (2006). Novel role for {alpha}vbeta5-integrin in retinal adhesion and its diurnal peak. Am. J. Physiol. Cell Physiol. 290: C1256-C1262 [Abstract] [Full Text]
Mora, R. C., Bonilha, V. L., Shin, B.-C., Hu, J., Cohen-Gould, L., Bok, D., Rodriguez-Boulan, E. (2006). Bipolar assembly of caveolae in retinal pigment epithelium. Am. J. Physiol. Cell Physiol. 290: C832-C843 [Abstract] [Full Text]
Strauss, O. (2005). The Retinal Pigment Epithelium in Visual Function. Physiol. Rev. 85: 845-881 [Abstract] [Full Text]
Zamiri, P., Masli, S., Kitaichi, N., Taylor, A. W., Streilein, J. W. (2005). Thrombospondin Plays a Vital Role in the Immune Privilege of the Eye. IOVS 46: 908-919 [Abstract] [Full Text]
Bonilha, V. L., Bhattacharya, S. K., West, K. A., Sun, J., Crabb, J. W., Rayborn, M. E., Hollyfield, J. G. (2004). Proteomic Characterization of Isolated Retinal Pigment Epithelium Microvilli. Mol. Cell. Proteomics 3: 1119-1127 [Abstract] [Full Text]
Deora, A. A., Gravotta, D., Kreitzer, G., Hu, J., Bok, D., Rodriguez-Boulan, E. (2004). The Basolateral Targeting Signal of CD147 (EMMPRIN) Consists of a Single Leucine and Is Not Recognized by Retinal Pigment Epithelium. Mol. Biol. Cell 15: 4148-4165 [Abstract] [Full Text]
Van Aken, E. H., De Wever, O., Van Hoorde, L., Bruyneel, E., De Laey, J.-J., Mareel, M. M. (2003). Invasion of Retinal Pigment Epithelial Cells: N-cadherin, Hepatocyte Growth Factor, and Focal Adhesion Kinase. IOVS 44: 463-472 [Abstract] [Full Text]
Peng, S., Rahner, C., Rizzolo, L. J. (2003). Apical and Basal Regulation of the Permeability of the Retinal Pigment Epithelium. IOVS 44: 808-817 [Abstract] [Full Text]
Geisert, E. E. Jr, Abel, H. J., Fan, L., Geisert, G. R. (2002). Retinal Pigment Epithelium of the Rat Express CD81, the Target of the Anti-proliferative Antibody (TAPA). IOVS 43: 274-280 [Abstract] [Full Text]
Bonilha, V. L., Rodriguez-Boulan, E. (2001). Polarity and Developmental Regulation of Two PDZ Proteins in the Retinal Pigment Epithelium. IOVS 42: 3274-3282 [Abstract] [Full Text]
Burke, J. M., Cao, F., Irving, P. E. (2000). High Levels of E-/P-Cadherin: Correlation with Decreased Apical Polarity of Na/K ATPase in Bovine RPE Cells In Situ. IOVS 41: 1945-1952 [Abstract] [Full Text]
Bonilha, V. L., Finnemann, S. C., Rodriguez-Boulan, E. (1999). Ezrin Promotes Morphogenesis of Apical Microvilli and Basal Infoldings in Retinal Pigment Epithelium. JCB 147: 1533-1548 [Abstract] [Full Text]
Burke, J. M., Cao, F., Irving, P. E., Skumatz, C. M. B. (1999). Expression of E-Cadherin by Human Retinal Pigment Epithelium: Delayed Expression In Vitro. IOVS 40: 2963-2970 [Abstract] [Full Text]
Vijayakumar, S., Takito, J., Hikita, C., Al-Awqati, Q. (1999). Hensin Remodels the Apical Cytoskeleton and Induces Columnarization of Intercalated Epithelial Cells: Processes that Resemble Terminal Differentiation. JCB 144: 1057-1067 [Abstract] [Full Text]
Marmorstein, A. D., Gan, Y. C., Bonilha, V. L., Finnemann, S. C., Csaky, K. G., Rodriguez-Boulan, E. (1998). Apical Polarity of N-CAM and EMMPRIN in Retinal Pigment Epithelium Resulting from Suppression of Basolateral Signal Recognition. JCB 142: 697-710 [Abstract] [Full Text]
Al-Awqati, Q., Vijayakumar, S., Hikita, C., Chen, J., Takito, J. (1998). Phenotypic plasticity in the intercalated cell: the hensin pathway. Am. J. Physiol. Renal Physiol. 275: F183-F190 [Abstract] [Full Text]
Hof, W. v., Malik, A., Vijayakumar, S., Qiao, J., Adelsberg, J. v., Al-Awqati, Q. (1997). The Effect of Apical and Basolateral Lipids on the Function of the Band 3 Anion Exchange Protein. JCB 139: 941-949 [Abstract] [Full Text]
Le Gall, A. H., Powell, S. K., Yeaman, C. A., Rodriguez-Boulan, E. (1997). The Neural Cell Adhesion Molecule Expresses a Tyrosine-independent Basolateral Sorting Signal. J. Biol. Chem. 272: 4559-4567 [Abstract] [Full Text]
Bonilha, V., Marmorstein, A., Cohen-Gould, L, Rodriguez-Boulan, E (1997). Apical sorting of influenza hemagglutinin by transcytosis in retinal pigment epithelium. J. Cell Sci. 110: 1717-1727 [Abstract]
Daston, M. M., Bastmeyer, M., Rutishauser, U., O'Leary, D. D.�M. (1996). Spatially Restricted Increase in Polysialic Acid Enhances Corticospinal Axon Branching Related to Target Recognition and Innervation. J. Neurosci. 16: 5488-5497 [Abstract] [Full Text]
Marmorstein, A., Bonilha, V., Chiflet, S, Neill, J., Rodriguez-Boulan, E (1996). The polarity of the plasma membrane protein RET-PE2 in retinal pigment epithelium is developmentally regulated. J. Cell Sci. 109: 3025-3034 [Abstract]
Mellman, I. (1995). Molecular Sorting of Membrane Proteins in Polarized and Nonpolarized Cells. Cold Spring Harb Symp Quant Biol 60: 745-752 [Abstract]