The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy

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The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy

M Itoh, A Nagafuchi, S Yonemura, T Kitani-Yasuda, S Tsukita and S Tsukita
Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.

We previously identified a 220-kD constitutive protein of the plasma membrane undercoat which colocalizes at the immunofluorescence microscopic level with cadherins and occurs not only in epithelial M., S. Yonemura, A. Nagafuchi, Sa. Tsukita, and Sh. Tsukita. 1991. J. Cell Biol. 115:1449-1462). To clarify the nature and possible functions of this protein, we cloned its full-length cDNA and sequenced it. Unexpectedly, we found mouse 220-kD protein to be highly homologous to rat protein ZO-1, only a part of which had been already sequenced. This relationship was confirmed by immunoblotting with anti-ZO-1 antibody. As protein ZO-1 was originally identified as a component exclusively underlying tight junctions in epithelial cells, where cadherins are not believed to be localized, we analyzed the distribution of cadherins and the 220-kD protein by ultrathin cryosection immunoelectron microscopy. We found that in non-epithelial cells lacking tight junctions cadherins and the 220-kD protein colocalize, whereas in epithelial cells (e.g., intestinal epithelial cells) bearing well-developed tight junctions cadherins and the 220-kD protein are clearly segregated into adherens and tight junctions, respectively. Interestingly, in epithelial cells such as hepatocytes, which tight junctions are not so well developed, the 220-kD protein is detected not only in the tight junction zone but also at adherens junctions. Furthermore, we show in mouse L cells transfected with cDNAs encoding N-, P-, E-cadherins that cadherins interact directly or indirectly with the 220-kD protein. Possible functions of the 220-kD protein (ZO-1) are discussed with special reference to the molecular mechanism for adherens and tight junction formation.
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Lee, D. B. N., Huang, E., Ward, H. J. (2006). Tight junction biology and kidney dysfunction. Am. J. Physiol. Renal Physiol. 290: F20-F34 [Abstract] [Full Text]
Powell, K. (2005). Sticking it out with tight junctions. JCB 171: 916-917 [Full Text]
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Osler, M. E., Chang, M. S., Bader, D. M. (2005). Bves modulates epithelial integrity through an interaction at the tight junction. J. Cell Sci. 118: 4667-4678 [Abstract] [Full Text]
Polette, M., Gilles, C., Nawrocki-Raby, B., Lohi, J., Hunziker, W., Foidart, J.-M., Birembaut, P. (2005). Membrane-Type 1 Matrix Metalloproteinase Expression Is Regulated by Zonula Occludens-1 in Human Breast Cancer Cells. Cancer Res. 65: 7691-7698 [Abstract] [Full Text]
Hawkins, B. T., Davis, T. P. (2005). The Blood-Brain Barrier/Neurovascular Unit in Health and Disease. Pharmacol. Rev. 57: 173-185 [Abstract] [Full Text]
Palmer, C. L., Cotton, L., Henley, J. M. (2005). The Molecular Pharmacology and Cell Biology of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors. Pharmacol. Rev. 57: 253-277 [Abstract] [Full Text]
Smalley, K. S.M., Brafford, P., Haass, N. K., Brandner, J. M., Brown, E., Herlyn, M. (2005). Up-Regulated Expression of Zonula Occludens Protein-1 in Human Melanoma Associates with N-Cadherin and Contributes to Invasion and Adhesion. Am. J. Pathol. 166: 1541-1554 [Abstract] [Full Text]
Vejlsted, M., Avery, B., Schmidt, M., Greve, T., Alexopoulos, N., Maddox-Hyttel, P. (2005). Ultrastructural and Immunohistochemical Characterization of the Bovine Epiblast. Biol. Reprod. 72: 678-686 [Abstract] [Full Text]
Roux, K. J., Amici, S. A., Fletcher, B. S., Notterpek, L. (2005). Modulation of Epithelial Morphology, Monolayer Permeability, and Cell Migration by Growth Arrest Specific 3/Peripheral Myelin Protein 22. Mol. Biol. Cell 16: 1142-1151 [Abstract] [Full Text]
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Sorgen, P. L., Duffy, H. S., Sahoo, P., Coombs, W., Delmar, M., Spray, D. C. (2004). Structural Changes in the Carboxyl Terminus of the Gap Junction Protein Connexin43 Indicates Signaling between Binding Domains for c-Src and Zonula Occludens-1. J. Biol. Chem. 279: 54695-54701 [Abstract] [Full Text]
Bordin, M., D'Atri, F., Guillemot, L., Citi, S. (2004). Histone Deacetylase Inhibitors Up-Regulate the Expression of Tight Junction Proteins. Mol Cancer Res 2: 692-701 [Abstract] [Full Text]
Kausalya, P. J., Phua, D. C.Y., Hunziker, W. (2004). Association of ARVCF with Zonula Occludens (ZO)-1 and ZO-2: Binding to PDZ-Domain Proteins and Cell-Cell Adhesion Regulate Plasma Membrane and Nuclear Localization of ARVCF. Mol. Biol. Cell 15: 5503-5515 [Abstract] [Full Text]
Kawkitinarong, K., Linz-McGillem, L., Birukov, K. G., Garcia, J. G. N. (2004). Differential Regulation of Human Lung Epithelial and Endothelial Barrier Function by Thrombin. Am. J. Respir. Cell Mol. Bio. 31: 517-527 [Abstract] [Full Text]
Gupta, S., Singh, R., Datta, P., Zhang, Z., Orr, C., Lu, Z., DuBois, G., Zervos, A. S., Meisler, M. H., Srinivasula, S. M., Fernandes-Alnemri, T., Alnemri, E. S. (2004). The C-terminal Tail of Presenilin Regulates Omi/HtrA2 Protease Activity. J. Biol. Chem. 279: 45844-45854 [Abstract] [Full Text]
Umeda, K., Matsui, T., Nakayama, M., Furuse, K., Sasaki, H., Furuse, M., Tsukita, S. (2004). Establishment and Characterization of Cultured Epithelial Cells Lacking Expression of ZO-1. J. Biol. Chem. 279: 44785-44794 [Abstract] [Full Text]
Bazzoni, G., Dejana, E. (2004). Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis. Physiol. Rev. 84: 869-901 [Abstract] [Full Text]
Schneeberger, E. E., Lynch, R. D. (2004). The tight junction: a multifunctional complex. Am. J. Physiol. Cell Physiol. 286: C1213-C1228 [Abstract] [Full Text]
Turksen, K., Troy, T.-C. (2004). Barriers built on claudins. J. Cell Sci. 117: 2435-2447 [Abstract] [Full Text]
Ohkubo, T., Ozawa, M. (2004). The transcription factor Snail downregulates the tight junction components independently of E-cadherin downregulation. J. Cell Sci. 117: 1675-1685 [Abstract] [Full Text]
Schuster, M. K., Schmierer, B., Shkumatava, A., Kuchler, K. (2004). Activin A and Follicle-Stimulating Hormone Control Tight Junctions in Avian Granulosa Cells by Regulating Occludin Expression. Biol. Reprod. 70: 1493-1499 [Abstract] [Full Text]
Giepmans, B. N.G (2004). Gap junctions and connexin-interacting proteins. Cardiovasc Res 62: 233-245 [Abstract] [Full Text]
Wang, X., Matsumoto, H., Zhao, X., Das, S. K., Paria, B. C. (2004). Embryonic signals direct the formation of tight junctional permeability barrier in the decidualizing stroma during embryo implantation. J. Cell Sci. 117: 53-62 [Abstract] [Full Text]
Geldmacher-Voss, B., Reugels, A. M., Pauls, S., Campos-Ortega, J. A. (2003). A 90{degrees} rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells. Development 130: 3767-3780 [Abstract] [Full Text]
Nielsen, P. A., Baruch, A., Shestopalov, V. I., Giepmans, B. N.G., Dunia, I., Benedetti, E. L., Kumar, N. M. (2003). Lens Connexins {alpha}3Cx46 and {alpha}8Cx50 Interact with Zonula Occludens Protein-1 (ZO-1). Mol. Biol. Cell 14: 2470-2481 [Abstract] [Full Text]
Ikenouchi, J., Matsuda, M., Furuse, M., Tsukita, S. (2003). Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116: 1959-1967 [Abstract] [Full Text]
Nie, G.-Y., Li, Y., Minoura, H., Batten, L., Ooi, G. T., Findlay, J. K., Salamonsen, L. A. (2003). A novel serine protease of the mammalian HtrA family is up-regulated in mouse uterus coinciding with placentation. Mol Hum Reprod 9: 279-290 [Abstract] [Full Text]
Burns, A. R., Smith, C. W., Walker, D. C. (2003). Unique Structural Features That Influence Neutrophil Emigration Into the Lung. Physiol. Rev. 83: 309-336 [Abstract] [Full Text]
Sasaki, H., Matsui, C., Furuse, K., Mimori-Kiyosue, Y., Furuse, M., Tsukita, S. (2003). Dynamic behavior of paired claudin strands within apposing plasma membranes. Proc. Natl. Acad. Sci. USA 100: 3971-3976 [Abstract] [Full Text]
Lui, W.-Y., Mruk, D., Lee, W. M, Cheng, C. Y. (2003). Sertoli Cell Tight Junction Dynamics: Their Regulation During Spermatogenesis. Biol. Reprod. 68: 1087-1097 [Abstract] [Full Text]
Balda, M. S., Garrett, M. D., Matter, K. (2003). The ZO-1-associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. JCB 160: 423-432 [Abstract] [Full Text]
Thi, M. M., Kojima, T., Cowin, S. C., Weinbaum, S., Spray, D. C. (2003). Fluid shear stress remodels expression and function of junctional proteins in cultured bone cells. Am. J. Physiol. Cell Physiol. 284: C389-C403 [Abstract] [Full Text]
Asada, M., Irie, K., Morimoto, K., Yamada, A., Ikeda, W., Takeuchi, M., Takai, Y. (2003). ADIP, a Novel Afadin- and alpha -Actinin-Binding Protein Localized at Cell-Cell Adherens Junctions. J. Biol. Chem. 278: 4103-4111 [Abstract] [Full Text]
You, Y., Richer, E. J., Huang, T., Brody, S. L. (2002). Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population. Am. J. Physiol. Lung Cell. Mol. Physiol. 283: L1315-L1321 [Abstract] [Full Text]
Spiers, A., Lamb, H. K., Cocklin, S., Wheeler, K. A., Budworth, J., Dodds, A. L., Pallen, M. J., Maskell, D. J., Charles, I. G., Hawkins, A. R. (2002). PDZ Domains Facilitate Binding of High Temperature Requirement Protease A (HtrA) and Tail-specific Protease (Tsp) to Heterologous Substrates through Recognition of the Small Stable RNA A (ssrA)-encoded Peptide. J. Biol. Chem. 277: 39443-39449 [Abstract] [Full Text]
Cheng, C. Y., Mruk, D. D. (2002). Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development. Physiol. Rev. 82: 825-874 [Abstract] [Full Text]
D'Atri, F., Nadalutti, F., Citi, S. (2002). Evidence for a Functional Interaction between Cingulin and ZO-1 in Cultured Cells. J. Biol. Chem. 277: 27757-27764 [Abstract] [Full Text]
Furuse, M., Hata, M., Furuse, K., Yoshida, Y., Haratake, A., Sugitani, Y., Noda, T., Kubo, A., Tsukita, S. (2002). Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. JCB 156: 1099-1111 [Abstract] [Full Text]
Nishimura, M., Kakizaki, M., Ono, Y., Morimoto, K., Takeuchi, M., Inoue, Y., Imai, T., Takai, Y. (2002). JEAP, a Novel Component of Tight Junctions in Exocrine Cells. J. Biol. Chem. 277: 5583-5587 [Abstract] [Full Text]
Kawabe, H., Nakanishi, H., Asada, M., Fukuhara, A., Morimoto, K., Takeuchi, M., Takai, Y. (2001). Pilt, a Novel Peripheral Membrane Protein at Tight Junctions in Epithelial Cells. J. Biol. Chem. 276: 48350-48355 [Abstract] [Full Text]
Itoh, M., Sasaki, H., Furuse, M., Ozaki, H., Kita, T., Tsukita, S. (2001). Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions. JCB 154: 491-498 [Abstract] [Full Text]
Yokoyama, S., Tachibana, K., Nakanishi, H., Yamamoto, Y., Irie, K., Mandai, K., Nagafuchi, A., Monden, M., Takai, Y. (2001). {alpha}-Catenin-independent Recruitment of ZO-1 to Nectin-based Cell-Cell Adhesion Sites through Afadin. Mol. Biol. Cell 12: 1595-1609 [Abstract] [Full Text]
Suzuki, T., Oyamada, M., Takamatsu, T. (2001). Different Regulation of Connexin26 and ZO-1 in Cochleas of Developing Rats and of Guinea Pigs with Endolymphatic Hydrops. J. Histochem. Cytochem. 49: 573-586 [Abstract] [Full Text]
Groos, S., Reale, E., Luciano, L. (2001). Re-evaluation of Epoxy Resin Sections for Light and Electron Microscopic Immunostaining. J. Histochem. Cytochem. 49: 397-406 [Abstract] [Full Text]
Decaens, C., Cassio, D. (2001). Spatiotemporal expression of catenins, ZO-1, and occludin during early polarization of hepatic WIF-B9 cells. Am. J. Physiol. Cell Physiol. 280: C527-C539 [Abstract] [Full Text]
Mo, C., Schneeberger, E. E., Arvin, A. M. (2000). Glycoprotein E of Varicella-Zoster Virus Enhances Cell-Cell Contact in Polarized Epithelial Cells. J. Virol. 74: 11377-11387 [Abstract] [Full Text]
Weed, S. A., Karginov, A. V., Schafer, D. A., Weaver, A. M., Kinley, A. W., Cooper, J. A., Parsons, J. T. (2000). Cortactin Localization to Sites of Actin Assembly in Lamellipodia Requires Interactions with F-Actin and the Arp2/3 Complex. JCB 151: 29-40 [Abstract] [Full Text]
Nevrivy, D. J., Peterson, V. J., Avram, D., Ishmael, J. E., Hansen, S. G., Dowell, P., Hruby, D. E., Dawson, M. I., Leid, M. (2000). Interaction of GRASP, a Protein encoded by a Novel Retinoic Acid-induced Gene, with Members of the Cytohesin Family of Guanine Nucleotide Exchange Factors. J. Biol. Chem. 275: 16827-16836 [Abstract] [Full Text]
Faccio, L., Fusco, C., Chen, A., Martinotti, S., Bonventre, J. V., Zervos, A. S. (2000). Characterization of a Novel Human Serine Protease That Has Extensive Homology to Bacterial Heat Shock Endoprotease HtrA and Is Regulated by Kidney Ischemia. J. Biol. Chem. 275: 2581-2588 [Abstract] [Full Text]
Sheth, B, Moran, B, Anderson, J., Fleming, T. (2000). Post-translational control of occludin membrane assembly in mouse trophectoderm: a mechanism to regulate timing of tight junction biogenesis and blastocyst formation. Development 127: 831-840 [Abstract]
REISER, J., KRIZ, W., KRETZLER, M., MUNDEL, P. (2000). The Glomerular Slit Diaphragm Is a Modified Adherens Junction. J. Am. Soc. Nephrol. 11: 1-8 [Abstract] [Full Text]
Trotter, K. W., Fraser, I. D.C., Scott, G. K., Stutts, M. J., Scott, J. D., Milgram, S. L. (1999). Alternative Splicing Regulates the Subcellular Localization of a-Kinase Anchoring Protein 18 Isoforms. JCB 147: 1481-1492 [Abstract] [Full Text]
Cordenonsi, M., D'Atri, F., Hammar, E., Parry, D. A.D., Kendrick-Jones, J., Shore, D., Citi, S. (1999). Cingulin Contains Globular and Coiled-Coil Domains and Interacts with Zo-1, Zo-2, Zo-3, and Myosin. JCB 147: 1569-1582 [Abstract] [Full Text]
Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. (1999). Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins. JCB 147: 1351-1363 [Abstract] [Full Text]
Thomas, M. K., Yao, K.-M., Tenser, M. S., Wong, G. G., Habener, J. F. (1999). Bridge-1, a Novel PDZ-Domain Coactivator of E2A-Mediated Regulation of Insulin Gene Transcription. Mol. Cell. Biol. 19: 8492-8504 [Abstract] [Full Text]
Hiiragi, T., Sasaki, H., Nagafuchi, A., Sabe, H., Shen, S. C., Matsuki, M., Yamanishi, K., Tsukita, S. (1999). Transglutaminase Type 1 and Its Cross-linking Activity Are Concentrated at Adherens Junctions in Simple Epithelial Cells. J. Biol. Chem. 274: 34148-34154 [Abstract] [Full Text]
Adams, L. D., Lemire, J. M., Schwartz, S. M. (1999). A Systematic Analysis of 40 Random Genes in Cultured Vascular Smooth Muscle Subtypes Reveals a Heterogeneity of Gene Expression and Identifies the Tight Junction Gene Zonula Occludens 2 as a Marker of Epithelioid "Pup" Smooth Muscle Cells and a Participant in Carotid Neointimal Formation. Arterioscler. Thromb. Vasc. Bio. 19: 2600-2608 [Abstract] [Full Text]
Ye, J., Tsukamoto, T., Sun, A., Nigam, S. K. (1999). A role for intracellular calcium in tight junction reassembly after ATP depletion-repletion. Am. J. Physiol. Renal Physiol. 277: F524-F532 [Abstract] [Full Text]
Pavlova, A., Stuart, R. O., Pohl, M., Nigam, S. K. (1999). Evolution of gene expression patterns in a model of branching morphogenesis. Am. J. Physiol. Renal Physiol. 277: F650-F663 [Abstract] [Full Text]
Ikeda, W., Nakanishi, H., Miyoshi, J., Mandai, K., Ishizaki, H., Tanaka, M., Togawa, A., Takahashi, K., Nishioka, H., Yoshida, H., Mizoguchi, A., Nishikawa, S.-i., Takai, Y. (1999). Afadin: A Key Molecule Essential for Structural Organization of Cell-Cell Junctions of Polarized Epithelia during Embryogenesis. JCB 146: 1117-1132 [Abstract] [Full Text]
Mitic, L. L., Schneeberger, E. E., Fanning, A. S., Anderson, J. M. (1999). Connexin-Occludin Chimeras Containing the Zo-Binding Domain of Occludin Localize at Mdck Tight Junctions and Nrk Cell Contacts. JCB 146: 683-693 [Abstract] [Full Text]
Truschel, S. T., Ruiz, W. G., Shulman, T., Pilewski, J., Sun, T.-T., Zeidel, M. L., Apodaca, G. (1999). Primary Uroepithelial Cultures. A MODEL SYSTEM TO ANALYZE UMBRELLA CELL BARRIER FUNCTION. J. Biol. Chem. 274: 15020-15029 [Abstract] [Full Text]
Morita, K., Sasaki, H., Fujimoto, K., Furuse, M., Tsukita, S. (1999). Claudin-11/OSP-based Tight Junctions of Myelin Sheaths in Brain and Sertoli Cells in Testis. JCB 145: 579-588 [Abstract] [Full Text]
Imamura, Y., Itoh, M., Maeno, Y., Tsukita, S., Nagafuchi, A. (1999). Functional Domains of {alpha}-Catenin Required for the Strong State of Cadherin-based Cell Adhesion. JCB 144: 1311-1322 [Abstract] [Full Text]
Itoh, M., Morita, K., Tsukita, S. (1999). Characterization of ZO-2 as a MAGUK Family Member Associated with Tight as well as Adherens Junctions with a Binding Affinity to Occludin and alpha �Catenin. J. Biol. Chem. 274: 5981-5986 [Abstract] [Full Text]
Morita, K., Furuse, M., Fujimoto, K., Tsukita, S. (1999). Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc. Natl. Acad. Sci. USA 96: 511-516 [Abstract] [Full Text]
YEAMAN, C., GRINDSTAFF, K. K., NELSON, W. J. (1999). New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79: 73-98 [Abstract] [Full Text]
Katsube, T., Takahisa, M., Ueda, R., Hashimoto, N., Kobayashi, M., Togashi, S. (1998). Cortactin Associates with the Cell-Cell Junction Protein ZO-1 in both Drosophila and Mouse. J. Biol. Chem. 273: 29672-29677 [Abstract] [Full Text]
Furuse, M., Sasaki, H., Fujimoto, K., Tsukita, S. (1998). A Single Gene Product, Claudin-1 or -2, Reconstitutes Tight Junction Strands and Recruits Occludin in Fibroblasts. JCB 143: 391-401 [Abstract] [Full Text]
Izumi, Y., Hirose, T., Tamai, Y., Hirai, S.-i., Nagashima, Y., Fujimoto, T., Tabuse, Y., Kemphues, K. J., Ohno, S. (1998). An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3. JCB 143: 95-106 [Abstract] [Full Text]
Taya, S., Yamamoto, T., Kano, K., Kawano, Y., Iwamatsu, A., Tsuchiya, T., Tanaka, K., Kanai-Azuma, M., Wood, S. A., Mattick, J. S., Kaibuchi, K. (1998). The Ras Target AF-6 is a Substrate of the Fam Deubiquitinating Enzyme. JCB 142: 1053-1062 [Abstract] [Full Text]
Gee, S. H., Sekely, S. A., Lombardo, C., Kurakin, A., Froehner, S. C., Kay, B. K. (1998). Cyclic Peptides as Non-carboxyl-terminal Ligands of Syntrophin PDZ Domains. J. Biol. Chem. 273: 21980-21987 [Abstract] [Full Text]
Hirao, K., Hata, Y., Ide, N., Takeuchi, M., Irie, M., Yao, I., Deguchi, M., Toyoda, A., Sudhof, T. C., Takai, Y. (1998). A Novel Multiple PDZ Domain-containing Molecule Interacting with N-Methyl-D-aspartate Receptors and Neuronal Cell Adhesion Proteins. J. Biol. Chem. 273: 21105-21110 [Abstract] [Full Text]
Ganzler-Odenthal, S. I.�I., Redies, C. (1998). Blocking N-Cadherin Function Disrupts the Epithelial Structure of Differentiating Neural Tissue in the Embryonic Chicken Brain. J. Neurosci. 18: 5415-5425 [Abstract] [Full Text]