NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

doi:10.1083/jcb.121.3.503

This Article

	Full Text (PDF, 2462K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Goh, P. Y.
	Articles by Kilmartin, J. V.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Goh, P. Y.
	Articles by Kilmartin, J. V.


Social Bookmarking

	What's this?

ARTICLES

NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

PY Goh and JV Kilmartin
MRC Laboratory of Molecular Biology, Cambridge, England.

A mutant, ndc10-1, was isolated by anti-tubulin staining of temperature- sensitive mutant banks of budding yeast. ndc10-1 has a defect chromosome segregation since chromosomes remains at one pole of the anaphase spindle. This produces one polyploid cell and one aploid cell, each containing a spindle pole body (SPD. NDC10 was cloned and sequenced and is identical to CBF2 (Jiang, W., J. Lechnermn and J. Carbon. 1993. J. Cell Biol. 121:513) which is the 110-kD component of a centromere DNA binding complex (Lechner, J., and J. Carbon. 1991. Cell. 61:717-725). NDC10 is an essential gene. Antibodies to Ndc10p labeled the SPB region in nearly all the cells examined including nonmitotic cells. In some cells with short spindles which may be in metaphase, staining was also observed along the spindle. The staining pattern and the phenotype of ndc10-1 are consistent with Cbf2p/Ndc10p being a kinetochore protein, and provide in vivo evidence for its role in the attachment of chromosomes to the spindle.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Bansal, P. K., Mishra, A., High, A. A., Abdulle, R., Kitagawa, K. (2009). Sgt1 Dimerization Is Negatively Regulated by Protein Kinase CK2-mediated Phosphorylation at Ser361. J. Biol. Chem. 284: 18692-18698 [Abstract] [Full Text]
Tartakoff, A. M., Jaiswal, P. (2009). Nuclear Fusion and Genome Encounter during Yeast Zygote Formation. Mol. Biol. Cell 20: 2932-2942 [Abstract] [Full Text]
Bansal, P. K., Nourse, A., Abdulle, R., Kitagawa, K. (2009). Sgt1 Dimerization Is Required for Yeast Kinetochore Assembly. J. Biol. Chem. 284: 3586-3592 [Abstract] [Full Text]
Scott, R. J., Cairo, L. V., Van de Vosse, D. W., Wozniak, R. W. (2009). The nuclear export factor Xpo1p targets Mad1p to kinetochores in yeast. JCB 184: 21-29 [Abstract] [Full Text]
Rahal, R., Amon, A. (2008). Mitotic CDKs control the metaphase-anaphase transition and trigger spindle elongation. Genes Dev. 22: 1534-1548 [Abstract] [Full Text]
Woodbury, E. L., Morgan, D. O. (2007). The Role of Self-association in Fin1 Function on the Mitotic Spindle. J. Biol. Chem. 282: 32138-32143 [Abstract] [Full Text]
Jaqaman, K., Dorn, J. F., Marco, E., Sorger, P. K., Danuser, G. (2007). Phenotypic clustering of yeast mutants based on kinetochore microtubule dynamics. Bioinformatics 23: 1666-1673 [Abstract] [Full Text]
Kerres, A., Jakopec, V., Fleig, U. (2007). The Conserved Spc7 Protein Is Required for Spindle Integrity and Links Kinetochore Complexes in Fission Yeast. Mol. Biol. Cell 18: 2441-2454 [Abstract] [Full Text]
Chiroli, E., Rossio, V., Lucchini, G., Piatti, S. (2007). The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects. JCB 177: 599-611 [Abstract] [Full Text]
Malik, H. S. (2006). A hitchhiker's guide to survival finally makes CENs. JCB 174: 747-749 [Abstract] [Full Text]
Montpetit, B., Hazbun, T. R., Fields, S., Hieter, P. (2006). Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation. JCB 174: 653-663 [Abstract] [Full Text]
Oakes, M. L., Johzuka, K., Vu, L., Eliason, K., Nomura, M. (2006). Expression of rRNA Genes and Nucleolus Formation at Ectopic Chromosomal Sites in the Yeast Saccharomyces cerevisiae.. Mol. Cell. Biol. 26: 6223-6238 [Abstract] [Full Text]
Kanta, H., Laprade, L., Almutairi, A., Pinto, I. (2006). Suppressor Analysis of a Histone Defect Identifies a New Function for the Hda1 Complex in Chromosome Segregation. Genetics 173: 435-450 [Abstract] [Full Text]
Kastenmayer, J. P., Ni, L., Chu, A., Kitchen, L. E., Au, W.-C., Yang, H., Carter, C. D., Wheeler, D., Davis, R. W., Boeke, J. D., Snyder, M. A., Basrai, M. A. (2006). Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae.. Genome Res 16: 365-373 [Abstract] [Full Text]
Gillis, A. N., Thomas, S., Hansen, S. D., Kaplan, K. B. (2005). A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis. JCB 171: 773-784 [Abstract] [Full Text]
Collins, K. A., Castillo, A. R., Tatsutani, S. Y., Biggins, S. (2005). De Novo Kinetochore Assembly Requires the Centromeric Histone H3 Variant. Mol. Biol. Cell 16: 5649-5660 [Abstract] [Full Text]
Bouck, D. C., Bloom, K. S. (2005). The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast. Proc. Natl. Acad. Sci. USA 102: 5408-5413 [Abstract] [Full Text]
Yoder, T. J., McElwain, M. A., Francis, S. E., Bagley, J., Muller, E. G.D., Pak, B., O'Toole, E. T., Winey, M., Davis, T. N. (2005). Analysis of a Spindle Pole Body Mutant Reveals a Defect in Biorientation and Illuminates Spindle Forces. Mol. Biol. Cell 16: 141-152 [Abstract] [Full Text]
Maiato, H., DeLuca, J., Salmon, E. D., Earnshaw, W. C. (2004). The dynamic kinetochore-microtubule interface. J. Cell Sci. 117: 5461-5477 [Abstract] [Full Text]
Stoyan, T., Carbon, J. (2004). Inner Kinetochore of the Pathogenic Yeast Candida glabrata. Eukaryot Cell 3: 1154-1163 [Abstract] [Full Text]
Bansal, P. K., Abdulle, R., Kitagawa, K. (2004). Sgt1 Associates with Hsp90: an Initial Step of Assembly of the Core Kinetochore Complex. Mol. Cell. Biol. 24: 8069-8079 [Abstract] [Full Text]
Capiaghi, C., Ho, T. V., Thoma, F. (2004). Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres. Mol. Cell. Biol. 24: 6907-6918 [Abstract] [Full Text]
Melloy, P. G., Holloway, S. L. (2004). Changes in the Localization of the Saccharomyces cerevisiae Anaphase-Promoting Complex Upon Microtubule Depolymerization and Spindle Checkpoint Activation. Genetics 167: 1079-1094 [Abstract] [Full Text]
Gillett, E. S., Espelin, C. W., Sorger, P. K. (2004). Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast. JCB 164: 535-546 [Abstract] [Full Text]
De Wulf, P., McAinsh, A. D., Sorger, P. K. (2003). Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 17: 2902-2921 [Abstract] [Full Text]
Nekrasov, V. S., Smith, M. A., Peak-Chew, S., Kilmartin, J. V. (2003). Interactions between Centromere Complexes in Saccharomyces cerevisiae. Mol. Biol. Cell 14: 4931-4946 [Abstract] [Full Text]
Espelin, C. W., Simons, K. T., Harrison, S. C., Sorger, P. K. (2003). Binding of the Essential Saccharomyces cerevisiae Kinetochore Protein Ndc10p to CDEII. Mol. Biol. Cell 14: 4557-4568 [Abstract] [Full Text]
Pearson, C. G., Maddox, P. S., Zarzar, T. R., Salmon, E.D., Bloom, K. (2003). Yeast Kinetochores Do Not Stabilize Stu2p-dependent Spindle Microtubule Dynamics. Mol. Biol. Cell 14: 4181-4195 [Abstract] [Full Text]
Kerscher, O., Crotti, L. B., Basrai, M. A. (2003). Recognizing Chromosomes in Trouble: Association of the Spindle Checkpoint Protein Bub3p with Altered Kinetochores and a Unique Defective Centromere. Mol. Cell. Biol. 23: 6406-6418 [Abstract] [Full Text]
Trelles-Sticken, E., Loidl, J., Scherthan, H. (2003). Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres. J. Cell Sci. 116: 2431-2442 [Abstract] [Full Text]
Buvelot, S., Tatsutani, S. Y., Vermaak, D., Biggins, S. (2003). The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. JCB 160: 329-339 [Abstract] [Full Text]
Gentry, M. S., Hallberg, R. L. (2002). Localization of Saccharomyces cerevisiae Protein Phosphatase 2A Subunits throughout Mitotic Cell Cycle. Mol. Biol. Cell 13: 3477-3492 [Abstract] [Full Text]
Mehta, S., Yang, X. M., Chan, C. S., Dobson, M. J., Jayaram, M., Velmurugan, S. (2002). The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?. JCB 158: 625-637 [Abstract] [Full Text]
Abruzzi, K. C., Magendantz, M., Solomon, F. (2002). An {alpha}-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae. Genetics 161: 983-994 [Abstract] [Full Text]
Maiato, H., Sampaio, P., Lemos, C. L., Findlay, J., Carmena, M., Earnshaw, W. C., Sunkel, C. E. (2002). MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. JCB 157: 749-760 [Abstract] [Full Text]
Cheeseman, I. M., Drubin, D. G., Barnes, G. (2002). Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast. JCB 157: 199-203 [Abstract] [Full Text]
Euskirchen, G. M. (2002). Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae. Eukaryot Cell 1: 229-240 [Abstract] [Full Text]
Li, Y., Bachant, J., Alcasabas, A. A., Wang, Y., Qin, J., Elledge, S. J. (2002). The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16: 183-197 [Abstract] [Full Text]
Sharp, J. A., Franco, A. A., Osley, M. A., Kaufman, P. D. (2002). Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev. 16: 85-100 [Abstract] [Full Text]
Measday, V., Hailey, D. W., Pot, I., Givan, S. A., Hyland, K. M., Cagney, G., Fields, S., Davis, T. N., Hieter, P. (2002). Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 16: 101-113 [Abstract] [Full Text]
Shanks, R. M. Q., Kamieniecki, R. J., Dawson, D. S. (2001). The Kar3-Interacting Protein Cik1p Plays a Critical Role in Passage Through Meiosis I in Saccharomyces cerevisiae. Genetics 159: 939-951 [Abstract] [Full Text]
Enquist-Newman, M., Cheeseman, I. M., Van Goor, D., Drubin, D. G., Meluh, P. B., Barnes, G. (2001). Dad1p, Third Component of the Duo1p/Dam1p Complex Involved in Kinetochore Function and Mitotic Spindle Integrity. Mol. Biol. Cell 12: 2601-2613 [Abstract] [Full Text]
Stoyan, T., Gloeckner, G., Diekmann, S., Carbon, J. (2001). Multifunctional Centromere Binding Factor 1 Is Essential for Chromosome Segregation in the Human Pathogenic Yeast Candida glabrata. Mol. Cell. Biol. 21: 4875-4888 [Abstract] [Full Text]
Adams, R. R., Maiato, H., Earnshaw, W. C., Carmena, M. (2001). Essential Roles of Drosophila Inner Centromere Protein (Incenp) and Aurora B in Histone H3 Phosphorylation, Metaphase Chromosome Alignment, Kinetochore Disjunction, and Chromosome Segregation. JCB 153: 865-880 [Abstract] [Full Text]
Gardner, R. D., Poddar, A., Yellman, C., Tavormina, P. A., Monteagudo, M. C., Burke, D. J. (2001). The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain. Genetics 157: 1493-1502 [Abstract] [Full Text]
Wigge, P. A., Kilmartin, J. V. (2001). The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation. JCB 152: 349-360 [Abstract] [Full Text]
Jensen, S., Segal, M., Clarke, D. J., Reed, S. I. (2001). A Novel Role of the Budding Yeast Separin Esp1 in Anaphase Spindle Elongation: Evidence That Proper Spindle Association of Esp1 Is Regulated by Pds1. JCB 152: 27-40 [Abstract] [Full Text]
Cheeseman, I. M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D. G., Barnes, G. (2001). Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex. JCB 152: 197-212 [Abstract] [Full Text]
Volkert, M. R., Elliott, N. A., Housman, D. E. (2000). Functional genomics reveals a family of eukaryotic oxidation protection genes. Proc. Natl. Acad. Sci. USA 10.1073/pnas.260495897v1 [Abstract] [Full Text]
Lin, H., Choi, J. H., Hasek, J., DeLillo, N., Lou, W., Vancura, A. (2000). Phospholipase C Is Involved in Kinetochore Function in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 3597-3607 [Abstract] [Full Text]
Jin, Q., Fuchs, J, Loidl, J (2000). Centromere clustering is a major determinant of yeast interphase nuclear organization. J. Cell Sci. 113: 1903-1912 [Abstract]
Zheng, L., Chen, Y., Lee, W.-H. (1999). Hec1p, an Evolutionarily Conserved Coiled-Coil Protein, Modulates Chromosome Segregation through Interaction with SMC Proteins. Mol. Cell. Biol. 19: 5417-5428 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Kim, J.-h., Kang, J.-s., Chan, C. S.M. (1999). Sli15 Associates with the Ipl1 Protein Kinase to Promote Proper Chromosome Segregation in Saccharomyces cerevisiae. JCB 145: 1381-1394 [Abstract] [Full Text]
Russell, I. D., Grancell, A. S., Sorger, P. K. (1999). The Unstable F-box Protein p58-Ctf13 Forms the Structural Core of the CBF3 Kinetochore Complex. JCB 145: 933-950 [Abstract] [Full Text]
Ortiz, J., Stemmann, O., Rank, S., Lechner, J. (1999). A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 13: 1140-1155 [Abstract] [Full Text]
Hyland, K. M., Kingsbury, J., Koshland, D., Hieter, P. (1999). Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle. JCB 145: 15-28 [Abstract] [Full Text]
Pietrasanta, L. I., Thrower, D., Hsieh, W., Rao, S., Stemmann, O., Lechner, J., Carbon, J., Hansma, H. (1999). Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3�(CBF3) kinetochore complex by using atomic force microscopy. Proc. Natl. Acad. Sci. USA 96: 3757-3762 [Abstract] [Full Text]
Biggins, S., Severin, F. F., Bhalla, N., Sassoon, I., Hyman, A. A., Murray, A. W. (1999). The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev. 13: 532-544 [Abstract] [Full Text]
Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M.-R., Kaplan, K. B., Ashford, A. J., Stark, M. J.R., Sorger, P. K., Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1�phosphatase Glc7p. Genes Dev. 13: 545-555 [Abstract] [Full Text]
Skibbens, R. V., Corson, L. B., Koshland, D., Hieter, P. (1999). Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev. 13: 307-319 [Abstract] [Full Text]
Dahlseid, J. N., Puziss, J., Shirley, R. L., Atkin, A. L., Hieter, P., Culbertson, M. R. (1998). Accumulation of mRNA Coding for the Ctf13p Kinetochore Subunit of Saccharomyces cerevisiae Depends on the Same Factors That Promote Rapid Decay of Nonsense mRNAs. Genetics 150: 1019-1035 [Abstract] [Full Text]
Wigge, P. A., Jensen, O. N., Holmes, S., Soues, S., Mann, M., Kilmartin, J. V. (1998). Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. JCB 141: 967-977 [Abstract] [Full Text]
Baker, R. E., Harris, K., Zhang, K. (1998). Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components. Genetics 149: 73-85 [Abstract] [Full Text]
Brachat, A., Kilmartin, J. V., Wach, A., Philippsen, P. (1998). Saccharomyces cerevisiae Cells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge-organized Microtubules. Mol. Biol. Cell 9: 977-991 [Abstract] [Full Text]
Tavormina, P. A., Burke, D. J. (1998). Cell Cycle Arrest in cdc20 Mutants of Saccharomyces cerevisiae Is Independent of Ndc10p and Kinetochore Function but Requires a Subset of Spindle Checkpoint Genes. Genetics 148: 1701-1713 [Abstract] [Full Text]
Luca, F. C., Winey, M. (1998). MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy. Mol. Biol. Cell 9: 29-46 [Abstract] [Full Text]
Espelin, C. W., Kaplan, K. B., Sorger, P. K. (1997). Probing the Architecture of a Simple Kinetochore Using DNA-Protein Crosslinking. JCB 139: 1383-1396 [Abstract] [Full Text]
Meluh, P. B., Koshland, D. (1997). Budding yeast centromere composition and assembly as revealed by in vivo�cross-linking. Genes Dev. 11: 3401-3412 [Abstract] [Full Text]
Saunders, W., Hornack, D., Lengyel, V., Deng, C. (1997). The Saccharomyces cerevisiae Kinesin-related Motor Kar3p Acts at Preanaphase Spindle Poles to Limit the Number and Length of Cytoplasmic Microtubules. JCB 137: 417-431 [Abstract] [Full Text]
Zarzov, P, Boucherie, H, Mann, C (1997). A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J. Cell Sci. 110: 1879-1891 [Abstract]
Li, Y., Benezra, R. (1996). Identification of a Human Mitotic Checkpoint Gene: hsMAD2. Science 274: 246-248 [Abstract] [Full Text]
Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G., Earnshaw, W. C. (1995). The Centromere: Hub of Chromosomal Activities. Science 270: 1591-1594 [Abstract]
Stoler, S, Keith, K C, Curnick, K E, Fitzgerald-Hayes, M (1995). A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis.. Genes Dev. 9: 573-586 [Abstract]
Brock, J., Bloom, K (1994). A chromosome breakage assay to monitor mitotic forces in budding yeast. J. Cell Sci. 107: 891-902 [Abstract]
Jiang, W., Carbon, J. (1993). Molecular Analysis of the Budding Yeast Centromere/Kinetochore. Cold Spring Harb Symp Quant Biol 58: 669-676 [Abstract]
Guacci, V., Yamamoto, A., Strunnikov, A., Kingsbury, J., Hogan, E., Meluh, P., Koshland, D. (1993). Structure and Function of Chromosomes in Mitosis of Budding Yeast. Cold Spring Harb Symp Quant Biol 58: 677-685 [Abstract]
Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 98: 13675-13680 [Abstract] [Full Text]
Volkert, M. R., Elliott, N. A., Housman, D. E. (2000). Functional genomics reveals a family of eukaryotic oxidation protection genes. Proc. Natl. Acad. Sci. USA 97: 14530-14535 [Abstract] [Full Text]
Cheeseman, I. M., Brew, C., Wolyniak, M., Desai, A., Anderson, S., Muster, N., Yates, J. R., Huffaker, T. C., Drubin, D. G., Barnes, G. (2001). Implication of a novel multiprotein Dam1p complex in outer kinetochore function. JCB 155: 1137-1146 [Abstract] [Full Text]
Cheeseman, I. M., Drubin, D. G., Barnes, G. (2002). Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast. JCB 157: 199-203 [Abstract] [Full Text]