Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore

doi:10.1083/jcb.121.3.513

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Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore

W Jiang, J Lechner and J Carbon
Department of Biological Sciences, University of California, Santa Barbara 93106.

We have cloned and determined the nucleotide sequence of the gene (CBF2) specifying the large (110 kD) subunit of the 240-kD multisubunit yeast centromere binding factor CBF3, which binds selectively in vitro to yeast centromere DNA and contains a minus end-directed microtubule motor activity. The deduced amino acid sequence of CBF2p shows no sequence homologies with known molecular motors, although a consensus nucleotide binding site is present. The CBF2 gene is essential for viability of yeast and is identical to NDC10, in which a conditional mutation leads to a defect in chromosome segregation (Goh, P.-Y., and J. V. Kilmartin, in this issue of The Journal of Cell Biology). The combined in vitro and in vivo evidence indicate that CBF2p is a key component of the budding yeast kinetochore.
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