Fibronectin binding site in type I collagen regulates fibronectin fibril formation

doi:10.1083/jcb.121.5.1165

This Article

	Full Text (PDF, 2730K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Dzamba, B. J.
	Articles by Peters, D. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Dzamba, B. J.
	Articles by Peters, D. M.


Social Bookmarking

	What's this?

ARTICLES

Fibronectin binding site in type I collagen regulates fibronectin fibril formation

BJ Dzamba, H Wu, R Jaenisch and DM Peters
Department of Laboratory Medicine and Pathology, University of Wisconsin, Madison 53706.

Mov13 fibroblasts, which do not express endogenous alpha 1(I) collagen chains due to a retroviral insertion, were used to study the role of type I collagen in the process of fibronectin fibrillogenesis. While Mov13 cells produced a sparse matrix containing short fibronectin fibrils, transfection with a wild type pro alpha 1(I) collagen gene resulted in the production of an extensive matrix containing fibronectin fibrils of normal length. To study the amino acids involved in the fibronectin-collagen interaction, mutations were introduced into the known fibronectin binding region of the pro alpha 1(I) collagen gene. Substitution of Gln and Ala at positions 774 and 777 of the alpha 1(I) chain for Pro resulted in the formation of short fibronectin fibrils similar to what was observed in untransfected Mov13 cells. Type I collagen carrying these substitutions bound weakly to fibronectin- sepharose and could be eluted off with 1 M urea. The effect of this mutation on fibronectin fibrillogenesis could be rescued by adding either type I collagen or a peptide fragment (CB.7) which contained the wild type fibronectin binding region of the alpha 1(I) chain to the cell culture. These results suggest that fibronectin fibrillogenesis in tissue culture is dependent on type I collagen synthesis, and define an important role for the fibronectin binding site in this process.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sottile, J., Shi, F., Rublyevska, I., Chiang, H.-Y., Lust, J., Chandler, J. (2007). Fibronectin-dependent collagen I deposition modulates the cell response to fibronectin. Am. J. Physiol. Cell Physiol. 293: C1934-C1946 [Abstract] [Full Text]
Zoppi, N., Gardella, R., De Paepe, A., Barlati, S., Colombi, M. (2004). Human Fibroblasts with Mutations in COL5A1 and COL3A1 Genes Do Not Organize Collagens and Fibronectin in the Extracellular Matrix, Down-regulate {alpha}2{beta}1 Integrin, and Recruit {alpha}v{beta}3 Instead of {alpha}5{beta}1 Integrin. J. Biol. Chem. 279: 18157-18168 [Abstract] [Full Text]
Lindsey, M. L., Yoshioka, J., MacGillivray, C., Muangman, S., Gannon, J., Verghese, A., Aikawa, M., Libby, P., Krane, S. M., Lee, R. T. (2003). Effect of a Cleavage-Resistant Collagen Mutation on Left Ventricular Remodeling. Circ. Res. 93: 238-245 [Abstract] [Full Text]
Velling, T., Risteli, J., Wennerberg, K., Mosher, D. F., Johansson, S. (2002). Polymerization of Type I and III Collagens Is Dependent On Fibronectin and Enhanced By Integrins alpha 11beta 1 and alpha 2beta 1. J. Biol. Chem. 277: 37377-37381 [Abstract] [Full Text]
Di Lullo, G. A., Sweeney, S. M., Korkko, J., Ala-Kokko, L., San Antonio, J. D. (2002). Mapping the Ligand-binding Sites and Disease-associated Mutations on the Most Abundant Protein in the Human, Type I Collagen. J. Biol. Chem. 277: 4223-4231 [Abstract] [Full Text]
Kinsella, M. G., Fischer, J. W., Mason, D. P., Wight, T. N. (2000). Retrovirally Mediated Expression of Decorin by Macrovascular Endothelial Cells. EFFECTS ON CELLULAR MIGRATION AND FIBRONECTIN FIBRILLOGENESIS IN VITRO. J. Biol. Chem. 275: 13924-13932 [Abstract] [Full Text]
Ohashi, T., Kiehart, D. P., Erickson, H. P. (1999). Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. Proc. Natl. Acad. Sci. USA 96: 2153-2158 [Abstract] [Full Text]
Rosenberg, K., Olsson, H., Morgelin, M., Heinegard, D. (1998). Cartilage Oligomeric Matrix Protein Shows High Affinity Zinc-dependent Interaction with Triple Helical Collagen. J. Biol. Chem. 273: 20397-20403 [Abstract] [Full Text]
Bultmann, H., Santas, A. J., Peters, D. M.�P. (1998). Fibronectin Fibrillogenesis Involves the Heparin II Binding Domain of Fibronectin. J. Biol. Chem. 273: 2601-2609 [Abstract] [Full Text]
Chernousov, M., Stahl, R., Carey, D. (1998). Schwann cells use a novel collagen-dependent mechanism for fibronectin fibril assembly. J. Cell Sci. 111: 2763-2777 [Abstract]
Grab, B., Miles, A. J., Furcht, L. T., Fields, G. B. (1996). Promotion of Fibroblast Adhesion by Triple-helical Peptide Models of Type I Collagen-derived Sequences. J. Biol. Chem. 271: 12234-12240 [Abstract] [Full Text]
Wu, C, Chung, A., McDonald, J. (1995). A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly. J. Cell Sci. 108: 2511-2523 [Abstract]
Ichihara-Tanaka, K, Titani, K, Sekiguchi, K (1995). Role of the carboxyl-terminal Fib2 domain in fibronectin matrix assembly. J. Cell Sci. 108: 907-915 [Abstract]