Signaling pathways for sphingosylphosphorylcholine-mediated mitogenesis in Swiss 3T3 fibroblasts

doi:10.1083/jcb.121.6.1385

This Article

	Full Text (PDF, 2263K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Desai, N. N.
	Articles by Spiegel, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Desai, N. N.
	Articles by Spiegel, S.


Social Bookmarking

	What's this?

ARTICLES

Signaling pathways for sphingosylphosphorylcholine-mediated mitogenesis in Swiss 3T3 fibroblasts

NN Desai, RO Carlson, ME Mattie, A Olivera, NE Buckley, T Seki, G Brooker and S Spiegel
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007.

Sphingosylphosphorylcholine (SPC), or lysophingomyelin, a wide-spectrum growth promoting agent for a variety of cell types (Desai, N. N., and S. Spiegel. 1991. Biochem. Biophys. Res. Comm. 181: 361-366), stimulates cellular proliferation of quiescent Swiss 3T3 fibroblasts to a greater extent than other known growth factors or than the structurally related molecules, sphingosine and sphingosine-1- phosphate. SPC potentiated the mitogenic effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol 13-acetate, and did not compete with phorbol esters for binding to protein kinase C in intact Swiss 3T3 fibroblasts. However, downregulation of protein kinase C, by prolonged treatment with phorbol ester, reduced, but did not eliminate, the ability of SPC to stimulate DNA synthesis, indicating that SPC may act via both protein kinase C-dependent and -independent signaling pathways. SPC induced a rapid rise in intracellular free calcium ([Ca2+]i) in viable 3T3 fibroblasts determined with a digital imaging system. Although the increases in [Ca2+]i were observed even in the absence of calcium in the external medium, no increase in the levels of inositol phosphates could be detected in response to mitogenic concentrations of SPC. Furthermore, in contrast to sphingosine or sphingosine-1-phosphate, the mitogenic effect of SPC was not accompanied by increases in phosphatidic acid levels or changes in cAMP levels. SPC, but not sphingosine or sphingosine-1-phosphate, stimulates the release of arachidonic acid. Therefore, the ability of SPC to act an extremely potent mitogen may be due to activation of signaling pathway(s) distinct from those used by sphingosine or sphingosine-1- phosphate.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Urs, A. N., Dammer, E., Sewer, M. B. (2006). Sphingosine Regulates the Transcription of CYP17 by Binding to Steroidogenic Factor-1. Endocrinology 147: 5249-5258 [Abstract] [Full Text]
Jeon, E. S., Song, H. Y., Kim, M. R., Moon, H. J., Bae, Y. C., Jung, J. S., Kim, J. H. (2006). Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK. J. Lipid Res. 47: 653-664 [Abstract] [Full Text]
Okamoto, R., Arikawa, J., Ishibashi, M., Kawashima, M., Takagi, Y., Imokawa, G. (2003). Sphingosylphosphorylcholine is upregulated in the stratum corneum of patients with atopic dermatitis. J. Lipid Res. 44: 93-102 [Abstract] [Full Text]
Shirao, S., Kashiwagi, S., Sato, M., Miwa, S., Nakao, F., Kurokawa, T., Todoroki-Ikeda, N., Mogami, K., Mizukami, Y., Kuriyama, S., Haze, K., Suzuki, M., Kobayashi, S. (2002). Sphingosylphosphorylcholine Is a Novel Messenger for Rho-Kinase-Mediated Ca2+ Sensitization in the Bovine Cerebral Artery: Unimportant Role for Protein Kinase C. Circ. Res. 91: 112-119 [Abstract] [Full Text]
Higuchi, K., Kawashima, M., Takagi, Y., Kondo, H., Yada, Y., Ichikawa, Y., Imokawa, G. (2001). Sphingosylphosphorylcholine is an activator of transglutaminase activity in human keratinocytes. J. Lipid Res. 42: 1562-1570 [Abstract] [Full Text]
Sullards, M. C., Merrill, A. H. Jr. (2001). Analysis of Sphingosine 1-Phosphate, Ceramides, and Other Bioactive Sphingolipids by High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Sci Signal 2001: pl1-pl1 [Abstract] [Full Text]
Xu, Y., Xiao, Y.-j., Baudhuin, L. M., Schwartz, B. M. (2001). The Role and Clinical Applications of Bioactive Lysolipids in Ovarian Cancer. Reproductive Sciences 8: 1-13 [Abstract]
Van Brocklyn, J. R., Graler, M. H., Bernhardt, G., Hobson, J. P., Lipp, M., Spiegel, S. (2000). Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6. Blood 95: 2624-2629 [Abstract] [Full Text]
Sekiguchi, K., Yokoyama, T., Kurabayashi, M., Okajima, F., Nagai, R. (1999). Sphingosylphosphorylcholine Induces a Hypertrophic Growth Response Through the Mitogen-Activated Protein Kinase Signaling Cascade in Rat Neonatal Cardiac Myocytes. Circ. Res. 85: 1000-1008 [Abstract] [Full Text]
Ancellin, N., Hla, T. (1999). Differential Pharmacological Properties and Signal Transduction of the Sphingosine 1-Phosphate Receptors EDG-1, EDG-3, and EDG-5. J. Biol. Chem. 274: 18997-19002 [Abstract] [Full Text]
Fadel, M. P., Dziak, E., Lo, C.-M., Ferrier, J., Mesaeli, N., Michalak, M., Opas, M. (1999). Calreticulin Affects Focal Contact-dependent but Not Close Contact-dependent Cell-substratum Adhesion. J. Biol. Chem. 274: 15085-15094 [Abstract] [Full Text]
Van Brocklyn, J. R., Tu, Z., Edsall, L. C., Schmidt, R. R., Spiegel, S. (1999). Sphingosine 1-Phosphate-induced Cell Rounding and Neurite Retraction Are Mediated by the G Protein-coupled Receptor H218. J. Biol. Chem. 274: 4626-4632 [Abstract] [Full Text]
Chin, T.-Y., Chueh, S.-H. (1998). Sphingosylphosphorylcholine stimulates mitogen-activated protein kinase via a Ca2+-dependent pathway. Am. J. Physiol. Cell Physiol. 275: C1255-C1263 [Abstract] [Full Text]
Liliom, K., Fischer, D. J., Virag, T., Sun, G., Miller, D. D., Tseng, J.-L., Desiderio, D. M., Seidel, M. C., Erickson, J. R., Tigyi, G. (1998). Identification of a Novel Growth Factor-like Lipid, 1-O-cis-Alk-1'-enyl-2-lyso-sn-glycero-3-phosphate (Alkenyl-GP) That Is Present in Commercial Sphingolipid Preparations. J. Biol. Chem. 273: 13461-13468 [Abstract] [Full Text]
Lee, M., Van Brocklyn, J. R., Thangada, S., Liu, C. H., Hand, A. R., Menzeleev, R., Spiegel, S., Hla, T. (1998). Sphingosine-1-Phosphate as a Ligand for the G Protein-Coupled Receptor EDG-1. Science 279: 1552-1555 [Abstract] [Full Text]
Titievsky, A., Titievskaya, I., Pasternack, M., Kaila, K., Tornquist, K. (1998). Sphingosine Inhibits Voltage-operated Calcium Channels in GH4C1 Cells. J. Biol. Chem. 273: 242-247 [Abstract] [Full Text]
Tornquist, K., Saarinen, P., Vainio, M., Ahlstrom, M. (1997). Sphingosine 1-Phosphate Mobilizes Sequestered Calcium, Activates Calcium Entry, and Stimulates Deoxyribonucleic Acid Synthesis in Thyroid FRTL-5 Cells. Endocrinology 138: 4049-4057 [Abstract] [Full Text]
Okajima, F., Kondo, Y. (1995). Pertussis Toxin Inhibits Phospholipase C Activation and Ca[IMAGE] Mobilization by Sphingosylphosphorylcholine and Galactosylsphingosine in HL60 Leukemia Cells. J. Biol. Chem. 270: 26332-26340 [Abstract] [Full Text]
Seufferlein, T., Rozengurt, E. (1995). Sphingosylphosphorylcholine Activation of Mitogen-activated Protein Kinase in Swiss 3T3 Cells Requires Protein Kinase C and a Pertussis Toxin-sensitive G Protein. J. Biol. Chem. 270: 24334-24342 [Abstract] [Full Text]
Seufferlein, T., Rozengurt, E. (1995). Sphingosylphosphorylcholine Rapidly Induces Tyrosine Phosphorylation of p125[IMAGE] and Paxillin, Rearrangement of the Actin Cytoskeleton and Focal Contact Assembly. J. Biol. Chem. 270: 24343-24351 [Abstract] [Full Text]
Kim, S., Lakhani, V., Costa, D. J., Sharara, A. I., Fitz, J. G., Huang, L.-W., Peters, K. G., Kindman, L. A. (1995). Sphingolipid-gated Ca[IMAGE] Release from Intracellular Stores of Endothelial Cells Is Mediated by a Novel Ca[IMAGE]-permeable Channel. J. Biol. Chem. 270: 5266-5269 [Abstract] [Full Text]
Zhu, K., Baudhuin, L. M., Hong, G., Williams, F. S., Cristina, K. L., Kabarowski, J. H. S., Witte, O. N., Xu, Y. (2001). Sphingosylphosphorylcholine and Lysophosphatidylcholine Are Ligands for the G Protein-coupled Receptor GPR4. J. Biol. Chem. 276: 41325-41335 [Abstract] [Full Text]
Berger, A., Crozier, G., Bisogno, T., Cavaliere, P., Innis, S., Di Marzo, V. (2001). Anandamide and diet: Inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines in piglets. Proc. Natl. Acad. Sci. USA 98: 6402-6406 [Abstract] [Full Text]