Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion

doi:10.1083/jcb.122.1.149

This Article

	Full Text (PDF, 2665K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Cooper, R.
	Articles by Cross, G. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Cooper, R.
	Articles by Cross, G. A.


Social Bookmarking

	What's this?

ARTICLES

Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion

R Cooper, AR de Jesus and GA Cross
Laboratory of Molecular Parasitology, Rockefeller University, New York 10021.

Null mutants of the Trypanosoma cruzi insect stage-specific glycoprotein GP72 were created by targeted gene replacement. Targeting plasmids were constructed in which the neomycin phosphotransferase and hygromycin phosphotransferase genes were flanked by GP72 sequences. These plasmids were sequentially transfected into T. cruzi epimastigotes by electroporation. Southern blot analyzes indicated that precise replacement of the two genes had occurred. No aberrant rearrangements occurred at the GP72 locus and no GP72 gene sequences had been translocated elsewhere in the genome. Western blots confirmed that GP72 is not expressed in these null mutants. The morphology of the mutants is dramatically different from wild-type. In both mutant and wild-type parasites, the flagellum emerges from the flagellar pocket. In the null mutant the normal attachment of the flagellum to the cell membrane of the parasite is lost.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Zago, M. P., Barrio, A. B., Cardozo, R. M., Duffy, T., Schijman, A. G., Basombrio, M. A. (2008). Impairment of Infectivity and Immunoprotective Effect of a LYT1 Null Mutant of Trypanosoma cruzi. Infect. Immun. 76: 443-451 [Abstract] [Full Text]
Turnock, D. C., Izquierdo, L., Ferguson, M. A. J. (2007). The de Novo Synthesis of GDP-fucose Is Essential for Flagellar Adhesion and Cell Growth in Trypanosoma brucei. J. Biol. Chem. 282: 28853-28863 [Abstract] [Full Text]
Atayde, V. D., Cortez, M., Souza, R., da Silveira, J. F., Yoshida, N. (2007). Expression and Cellular Localization of Molecules of the gp82 Family in Trypanosoma cruzi Metacyclic Trypomastigotes. Infect. Immun. 75: 3264-3270 [Abstract] [Full Text]
Heras, S. R., Lopez, M. C., Olivares, M., Thomas, M. C. (2007). The L1Tc non-LTR retrotransposon of Trypanosoma cruzi contains an internal RNA-pol II-dependent promoter that strongly activates gene transcription and generates unspliced transcripts. Nucleic Acids Res 35: 2199-2214 [Abstract] [Full Text]
Okura, M., Fang, J., Salto, M. L., Singer, R. S., Docampo, R., Moreno, S. N. J. (2005). A Lipid-modified Phosphoinositide-specific Phospholipase C (TcPI-PLC) Is Involved in Differentiation of Trypomastigotes to Amastigotes of Trypanosoma cruzi. J. Biol. Chem. 280: 16235-16243 [Abstract] [Full Text]
Briggs, L. J., McKean, P. G., Baines, A., Moreira-Leite, F., Davidge, J., Vaughan, S., Gull, K. (2004). The flagella connector of Trypanosoma brucei: an unusual mobile transmembrane junction. J. Cell Sci. 117: 1641-1651 [Abstract] [Full Text]
Hill, K. L. (2003). Biology and Mechanism of Trypanosome Cell Motility. Eukaryot Cell 2: 200-208 [Full Text]
LaCount, D. J., Barrett, B., Donelson, J. E. (2002). Trypanosoma brucei FLA1 Is Required for Flagellum Attachment and Cytokinesis. J. Biol. Chem. 277: 17580-17588 [Abstract] [Full Text]
Hutchings, N. R., Donelson, J. E., Hill, K. L. (2002). Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes. JCB 156: 867-877 [Abstract] [Full Text]
Moreira-Leite, F. F., Sherwin, T., Kohl, L., Gull, K. (2001). A Trypanosome Structure Involved in Transmitting Cytoplasmic Information During Cell Division. Science 294: 610-612 [Abstract] [Full Text]
Haynes, P., Russell, D., Cross, G. (1996). Subcellular localization of Trypanosoma cruzi glycoprotein Gp72. J. Cell Sci. 109: 2979-2988 [Abstract]
de Jesus, A., Cooper, R, Espinosa, M, Gomes, J., Garcia, E., Paul, S, Cross, G. (1993). Gene deletion suggests a role for Trypanosoma cruzi surface glycoprotein GP72 in the insect and mammalian stages of the life cycle. J. Cell Sci. 106: 1023-1033 [Abstract]
Nozaki, T., Shigeta, Y., Saito-Nakano, Y., Imada, M., Kruger, W. D. (2001). Characterization of Transsulfuration and Cysteine Biosynthetic Pathways in the Protozoan Hemoflagellate, Trypanosoma cruzi. ISOLATION AND MOLECULAR CHARACTERIZATION OF CYSTATHIONINE beta -SYNTHASE AND SERINE ACETYLTRANSFERASE FROM TRYPANOSOMA. J. Biol. Chem. 276: 6516-6523 [Abstract] [Full Text]
Hutchings, N. R., Donelson, J. E., Hill, K. L. (2002). Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes. JCB 156: 867-877 [Abstract] [Full Text]