The alpha v beta 1 integrin functions as a fibronectin receptor but does not support fibronectin matrix assembly and cell migration on fibronectin

doi:10.1083/jcb.122.1.235

This Article

	Full Text (PDF, 1595K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Zhang, Z.
	Articles by Ruoslahti, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Zhang, Z.
	Articles by Ruoslahti, E.


Social Bookmarking

	What's this?

ARTICLES

The alpha v beta 1 integrin functions as a fibronectin receptor but does not support fibronectin matrix assembly and cell migration on fibronectin

Z Zhang, AO Morla, K Vuori, JS Bauer, RL Juliano and E Ruoslahti
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.

The fibronectin receptor, alpha 5 beta 1, has been shown to be required for fibronectin matrix assembly and plays an important role in cell migration on fibronectin. However, it is not clear whether other fibronectin binding integrins can take the place of alpha 5 beta 1 during matrix assembly and cell migration. To test this, we expressed the human alpha v subunit in the CHO cell line CHO-B2 that lacks the alpha 5 subunit. We found that the human alpha v combined with CHO cell beta 1 to form the integrin alpha v beta 1. Cells that expressed alpha v beta 1 attached to and spread well on fibronectin-coated dishes, but did so less well on vitronectin-coated dishes. This, along with other data, indicated that alpha v beta 1 functions as a fibronectin receptor in CHO-B2 cells. The alpha v beta 1-expressing cells failed to produce a fibronectin matrix or to migrate on fibronectin, although the same cells transfected with alpha 5 do produce a matrix and migrate on fibronectin. The affinity of the alpha v beta 1-expressing cells for fibronectin was fourfold lower than that of the alpha 5 beta 1- expressing cells. In addition, alpha v beta 1 was distributed diffusely throughout the cell surface, whereas alpha 5 beta 1 was localized to focal adhesions when cells were seeded onto fibronectin-coated surfaces. Thus, of the two fibronectin receptors, alpha v beta 1 and alpha 5 beta 1, only alpha 5 beta 1 supports fibronectin matrix assembly and promotes cell migration on fibronectin in the CHO-B2 cells. Possible reasons for this difference in the activities of alpha v beta 1 and alpha 5 beta 1 include the lower affinity of alpha v beta 1 for fibronectin and the failure of this integrin to localize in adhesion plaques on a fibronectin substrate. These results show that two integrins with similar ligand specificities and cell attachment functions may be quite different in their ability to support fibronectin matrix assembly and cell motility on fibronectin.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ithychanda, S. S., Das, M., Ma, Y.-Q., Ding, K., Wang, X., Gupta, S., Wu, C., Plow, E. F., Qin, J. (2009). Migfilin, a Molecular Switch in Regulation of Integrin Activation. J. Biol. Chem. 284: 4713-4722 [Abstract] [Full Text]
Huveneers, S., Truong, H., Fassler, R., Sonnenberg, A., Danen, E. H. J. (2008). Binding of soluble fibronectin to integrin {alpha}5{beta}1 - link to focal adhesion redistribution and contractile shape. J. Cell Sci. 121: 2452-2462 [Abstract] [Full Text]
Sabella, C., Faszewski, E., Himic, L., Colpitts, K. M., Kaltenbach, J., Burger, M. M., Fernandez-Busquets, X. (2007). Cyclosporin A Suspends Transplantation Reactions in the Marine Sponge Microciona prolifera. J. Immunol. 179: 5927-5935 [Abstract] [Full Text]
Degen, M., Brellier, F., Kain, R., Ruiz, C., Terracciano, L., Orend, G., Chiquet-Ehrismann, R. (2007). Tenascin-W Is a Novel Marker for Activated Tumor Stroma in Low-grade Human Breast Cancer and Influences Cell Behavior. Cancer Res. 67: 9169-9179 [Abstract] [Full Text]
Somanath, P. R., Kandel, E. S., Hay, N., Byzova, T. V. (2007). Akt1 Signaling Regulates Integrin Activation, Matrix Recognition, and Fibronectin Assembly. J. Biol. Chem. 282: 22964-22976 [Abstract] [Full Text]
Lakhe-Reddy, S., Khan, S., Konieczkowski, M., Jarad, G., Wu, K. L., Reichardt, L. F., Takai, Y., Bruggeman, L. A., Wang, B., Sedor, J. R., Schelling, J. R. (2006). beta8 Integrin Binds Rho GDP Dissociation Inhibitor-1 and Activates Rac1 to Inhibit Mesangial Cell Myofibroblast Differentiation. J. Biol. Chem. 281: 19688-19699 [Abstract] [Full Text]
Mao, Y., Schwarzbauer, J. E. (2005). Stimulatory effects of a three-dimensional microenvironment on cell-mediated fibronectin fibrillogenesis. J. Cell Sci. 118: 4427-4436 [Abstract] [Full Text]
Liu, C., Dickinson, C., Shobe, J., Donate, F., Ruf, W., Edgington, T. (2004). A hybrid fibronectin motif protein as an integrin targeting selective tumor vascular thrombogen. Molecular Cancer Therapeutics 3: 793-801 [Abstract] [Full Text]
Wilson, S. H., Ljubimov, A. V., Morla, A. O., Caballero, S., Shaw, L. C., Spoerri, P. E., Tarnuzzer, R. W., Grant, M. B. (2003). Fibronectin Fragments Promote Human Retinal Endothelial Cell Adhesion and Proliferation and ERK Activation through {alpha}5{beta}1 Integrin and PI 3-Kinase. IOVS 44: 1704-1715 [Abstract] [Full Text]
Robinson, E. E., Zazzali, K. M., Corbett, S. A., Foty, R. A. (2003). {alpha}5{beta}1 integrin mediates strong tissue cohesion. J. Cell Sci. 116: 377-386 [Abstract] [Full Text]
Danen, E. H.J., Sonneveld, P., Brakebusch, C., Fassler, R., Sonnenberg, A. (2002). The fibronectin-binding integrins {alpha}5{beta}1 and {alpha}v{beta}3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis. JCB 159: 1071-1086 [Abstract] [Full Text]
Koistinen, P., Heino, J. (2002). The Selective Regulation of alpha Vbeta 1 Integrin Expression Is Based on the Hierarchical Formation of alpha V-containing Heterodimers. J. Biol. Chem. 277: 24835-24841 [Abstract] [Full Text]
Tang, H., Hao, Q., Fitzgerald, T., Sasaki, T., Landon, E. J., Inagami, T. (2002). Pyk2/CAKbeta Tyrosine Kinase Activity-mediated Angiogenesis of Pulmonary Vascular Endothelial Cells. J. Biol. Chem. 277: 5441-5447 [Abstract] [Full Text]
Jackson, T., Mould, A. P., Sheppard, D., King, A. M. Q. (2002). Integrin {alpha}v{beta}1 Is a Receptor for Foot-and-Mouth Disease Virus. J. Virol. 76: 935-941 [Abstract] [Full Text]
Kuhns, W. J., Rusciano, D., Kaltenbach, J., Ho, M., Burger, M., Fernandez-Busquets, X. (2001). Up-regulation of Integrins {alpha}3 {beta}1 in Sulfate-Starved Marine Sponge Cells: Functional Correlates. Biol. Bull. 201: 238-239 [Full Text]
Laukaitis, C. M., Webb, D. J., Donais, K., Horwitz, A. F. (2001). Differential Dynamics of {alpha}5 Integrin, Paxillin, and {alpha}-Actinin during Formation and Disassembly of Adhesions in Migrating Cells. JCB 153: 1427-1440 [Abstract] [Full Text]
Rehn, M., Veikkola, T., Kukk-Valdre, E., Nakamura, H., Ilmonen, M., Lombardo, C. R., Pihlajaniemi, T., Alitalo, K., Vuori, K. (2001). Interaction of endostatin with integrins implicated in angiogenesis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.031564998v1 [Abstract] [Full Text]
Pankov, R., Cukierman, E., Katz, B.-Z., Matsumoto, K., Lin, D. C., Lin, S., Hahn, C., Yamada, K. M. (2000). Integrin Dynamics and Matrix Assembly: Tensin-Dependent Translocation of {alpha}5{beta}1 Integrins Promotes Early Fibronectin Fibrillogenesis. JCB 148: 1075-1090 [Abstract] [Full Text]
Sottile, J, Hocking, D., Langenbach, K. (2000). Fibronectin polymerization stimulates cell growth by RGD-dependent and -independent mechanisms. J. Cell Sci. 113: 4287-4299 [Abstract]
Klass, C., Couchman, J., Woods, A (2000). Control of extracellular matrix assembly by syndecan-2 proteoglycan. J. Cell Sci. 113: 493-506 [Abstract]
Degryse, B., Resnati, M., Rabbani, S. A., Villa, A., Fazioli, F., Blasi, F. (1999). Src-Dependence and Pertussis-Toxin Sensitivity of Urokinase Receptor-Dependent Chemotaxis and Cytoskeleton Reorganization in Rat Smooth Muscle Cells. Blood 94: 649-662 [Abstract] [Full Text]
Zhang, Q., Sakai, T., Nowlen, J., Hayashi, I., Fassler, R., Mosher, D. F. (1999). Functional beta 1-Integrins Release the Suppression of Fibronectin Matrix Assembly by Vitronectin. J. Biol. Chem. 274: 368-375 [Abstract] [Full Text]
Bourdoulous, S., Orend, G., MacKenna, D. A., Pasqualini, R., Ruoslahti, E. (1998). Fibronectin Matrix Regulates Activation of RHO and CDC42 GTPases and Cell Cycle Progression. JCB 143: 267-276 [Abstract] [Full Text]
Magnusson, M. K., Mosher, D. F. (1998). Fibronectin : Structure, Assembly, and Cardiovascular Implications. Arterioscler. Thromb. Vasc. Bio. 18: 1363-1370 [Full Text]
Munger, J. S., Harpel, J. G., Giancotti, F. G., Rifkin, D. B. (1998). Interactions between Growth Factors and Integrins: Latent Forms of Transforming Growth Factor-beta Are Ligands for the Integrin alpha vbeta 1. Mol. Biol. Cell 9: 2627-2638 [Abstract] [Full Text]
Yu, D.-H., Qu, C.-K., Henegariu, O., Lu, X., Feng, G.-S. (1998). Protein-tyrosine Phosphatase Shp-2 Regulates Cell Spreading, Migration, and Focal Adhesion. J. Biol. Chem. 273: 21125-21131 [Abstract] [Full Text]
Matter, M. L., Zhang, Z., Nordstedt, C., Ruoslahti, E. (1998). The {alpha}5{beta}1 Integrin Mediates Elimination of Amyloid-{beta} Peptide and Protects Against Apoptosis. JCB 141: 1019-1030 [Abstract] [Full Text]
Enomoto-Iwamoto, M., Iwamoto, M., Mukudai, Y., Kawakami, Y., Nohno, T., Higuchi, Y., Takemoto, S., Ohuchi, H., Noji, S., Kurisu, K. (1998). Bone Morphogenetic Protein Signaling Is Required for Maintenance of Differentiated Phenotype, Control of Proliferation, and Hypertrophy in Chondrocytes. JCB 140: 409-418 [Abstract] [Full Text]
Wu, C., Keightley, S. Y., Leung-Hagesteijn, C., Radeva, G., Coppolino, M., Goicoechea, S., McDonald, J. A., Dedhar, S. (1998). Integrin-linked Protein Kinase Regulates Fibronectin Matrix Assembly, E-cadherin Expression, and Tumorigenicity. J. Biol. Chem. 273: 528-536 [Abstract] [Full Text]
Brakebusch, C, Hirsch, E, Potocnik, A, Fassler, R (1997). Genetic analysis of beta1 integrin function: confirmed, new and revised roles for a crucial family of cell adhesion molecules. J. Cell Sci. 110: 2895-2904 [Abstract]
Cattelino, A, Longhi, R, de Curtis, I (1995). Differential distribution of two cytoplasmic variants of the alpha 6 beta 1 integrin laminin receptor in the ventral plasma membrane of embryonic fibroblasts. J. Cell Sci. 108: 3067-3078 [Abstract]
Mitjans, F, Sander, D, Adan, J, Sutter, A, Martinez, J., Jaggle, C., Moyano, J., Kreysch, H., Piulats, J, Goodman, S. (1995). An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. J. Cell Sci. 108: 2825-2838 [Abstract]
Wu, C, Chung, A., McDonald, J. (1995). A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly. J. Cell Sci. 108: 2511-2523 [Abstract]
Wu, C, Fields, A., Kapteijn, B., McDonald, J. (1995). The role of alpha 4 beta 1 integrin in cell motility and fibronectin matrix assembly. J. Cell Sci. 108: 821-829 [Abstract]
Delannet, M., Martin, F., Bossy, B., Cheresh, D. A., Reichardt, L. F., Duband, J. L. (1994). Specific roles of the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins in avian neural crest cell adhesion and migration on vitronectin. Development 120: 2687-2702 [Abstract]
Carlson, T. R., Feng, Y., Maisonpierre, P. C., Mrksich, M., Morla, A. O. (2001). Direct Cell Adhesion to the Angiopoietins Mediated by Integrins. J. Biol. Chem. 276: 26516-26525 [Abstract] [Full Text]
Matter, M. L., Ruoslahti, E. (2001). A Signaling Pathway from the alpha 5beta 1 and alpha vbeta 3 Integrins That Elevates bcl-2 Transcription. J. Biol. Chem. 276: 27757-27763 [Abstract] [Full Text]
Rehn, M., Veikkola, T., Kukk-Valdre, E., Nakamura, H., Ilmonen, M., Lombardo, C. R., Pihlajaniemi, T., Alitalo, K., Vuori, K. (2001). Interaction of endostatin with integrins implicated in angiogenesis. Proc. Natl. Acad. Sci. USA 98: 1024-1029 [Abstract] [Full Text]