Cell type-dependent variations in the subcellular distribution of alpha- mannosidase I and II

doi:10.1083/jcb.122.1.39

This Article

	Full Text (PDF, 8248K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Velasco, A.
	Articles by Farquhar, M. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Velasco, A.
	Articles by Farquhar, M. G.


Social Bookmarking

	What's this?

ARTICLES

Cell type-dependent variations in the subcellular distribution of alpha- mannosidase I and II

A Velasco, L Hendricks, KW Moremen, DR Tulsiani, O Touster and MG Farquhar
Division of Cellular and Molecular Medicine and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0651.

alpha-mannosidases I and II (Man I and II) are resident enzymes of the Golgi complex involved in oligosaccharide processing during N-linked glycoprotein biosynthesis that are widely considered to be markers of the cis- and medial-Golgi compartments, respectively. We have investigated the distribution of these enzymes in several cell types by immunofluorescence and immunoelectron microscopy. Man II was most commonly found in medial- and/or trans- cisternae but showed cell type- dependent variations in intra-Golgi distribution. It was variously localized to either medial (NRK and CHO cells), both medial and trans (pancreatic acinar cells, enterocytes), or trans- (goblet cells) cisternae, or distributed across the entire Golgi stack (hepatocytes and some enterocytes). The distribution of Man I largely coincided with that of Man II in that it was detected primarily in medial- and trans- cisternae. It also showed cell type dependent variations in its intra- Golgi distribution. Man I and Man II were also detected within secretory granules and at the cell surface of some cell types (enterocytes, pancreatic acinar cells, goblet cells). In the case of Man II, cell surface staining was shown not to be due to antibody cross- reactivity with oligosaccharide epitopes. These results indicate that the distribution of Man I and Man II within the Golgi stack of a given cell type overlaps considerably, and their distribution from one cell type to another is more variable and less compartmentalized than previously assumed.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ghosh, P., Garcia-Marcos, M., Bornheimer, S. J., Farquhar, M. G. (2008). Activation of G{alpha}i3 triggers cell migration via regulation of GIV. JCB 182: 381-393 [Abstract] [Full Text]
Avezov, E., Frenkel, Z., Ehrlich, M., Herscovics, A., Lederkremer, G. Z. (2008). Endoplasmic Reticulum (ER) Mannosidase I Is Compartmentalized and Required for N-Glycan Trimming to Man5 6GlcNAc2 in Glycoprotein ER-associated Degradation. Mol. Biol. Cell 19: 216-225 [Abstract] [Full Text]
Yilmaz Dejgaard, S., Murshid, A., Dee, K. M., Presley, J. F. (2007). Confocal Microscopy-based Linescan Methodologies for Intra-Golgi Localization of Proteins. J. Histochem. Cytochem. 55: 709-719 [Abstract] [Full Text]
Gaietta, G. M., Giepmans, B. N. G., Deerinck, T. J., Smith, W. B., Ngan, L., Llopis, J., Adams, S. R., Tsien, R. Y., Ellisman, M. H. (2006). Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc. Natl. Acad. Sci. USA 103: 17777-17782 [Abstract] [Full Text]
Saint-Jore-Dupas, C., Nebenfuhr, A., Boulaflous, A., Follet-Gueye, M.-L., Plasson, C., Hawes, C., Driouich, A., Faye, L., Gomord, V. (2006). Plant N-Glycan Processing Enzymes Employ Different Targeting Mechanisms for Their Spatial Arrangement along the Secretory Pathway. Plant Cell 18: 3182-3200 [Abstract] [Full Text]
Zhao, W., Chen, T.-L. L., Vertel, B. M., Colley, K. J. (2006). The CMP-sialic Acid Transporter Is Localized in the Medial-Trans Golgi and Possesses Two Specific Endoplasmic Reticulum Export Motifs in Its Carboxyl-terminal Cytoplasmic Tail. J. Biol. Chem. 281: 31106-31118 [Abstract] [Full Text]
Grewal, P. K., McLaughlan, J. M., Moore, C. J., Browning, C. A., Hewitt, J. E. (2005). Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies. Glycobiology 15: 912-923 [Abstract] [Full Text]
Le-Niculescu, H., Niesman, I., Fischer, T., DeVries, L., Farquhar, M. G. (2005). Identification and Characterization of GIV, a Novel G{alpha}i/s -interacting Protein Found on COPI, Endoplasmic Reticulum-Golgi Transport Vesicles. J. Biol. Chem. 280: 22012-22020 [Abstract] [Full Text]
Malsam, J., Satoh, A., Pelletier, L., Warren, G. (2005). Golgin Tethers Define Subpopulations of COPI Vesicles. Science 307: 1095-1098 [Abstract] [Full Text]
Kweon, H.-S., Beznoussenko, G. V., Micaroni, M., Polishchuk, R. S., Trucco, A., Martella, O., Di Giandomenico, D., Marra, P., Fusella, A., Di Pentima, A., Berger, E. G., Geerts, W. J. C., Koster, A. J., Burger, K. N. J., Luini, A., Mironov, A. A. (2004). Golgi Enzymes Are Enriched in Perforated Zones of Golgi Cisternae but Are Depleted in COPI Vesicles. Mol. Biol. Cell 15: 4710-4724 [Abstract] [Full Text]
Duran, J. M., Cano, M., Peral, M. J., Ilundain, A. A. (2004). D-mannose transport and metabolism in isolated enterocytes. Glycobiology 14: 495-500 [Abstract] [Full Text]
Parker, A. K. T., Gergely, F. V., Taylor, C. W. (2004). Targeting of Inositol 1,4,5-Trisphosphate Receptors to the Endoplasmic Reticulum by Multiple Signals within Their Transmembrane Domains. J. Biol. Chem. 279: 23797-23805 [Abstract] [Full Text]
Pottekat, A., Menon, A. K. (2004). Subcellular Localization and Targeting of N-Acetylglucosaminyl Phosphatidylinositol De-N-acetylase, the Second Enzyme in the Glycosylphosphatidylinositol Biosynthetic Pathway. J. Biol. Chem. 279: 15743-15751 [Abstract] [Full Text]
Nagai, M., Meerloo, T., Takeda, T., Farquhar, M. G. (2003). The Adaptor Protein ARH Escorts Megalin to and through Endosomes. Mol. Biol. Cell 14: 4984-4996 [Abstract] [Full Text]
Frenkel, Z., Gregory, W., Kornfeld, S., Lederkremer, G. Z. (2003). Endoplasmic Reticulum-associated Degradation of Mammalian Glycoproteins Involves Sugar Chain Trimming to Man6-5GlcNAc2. J. Biol. Chem. 278: 34119-34124 [Abstract] [Full Text]
Alvarez, C., Garcia-Mata, R., Brandon, E., Sztul, E. (2003). COPI Recruitment Is Modulated by a Rab1b-dependent Mechanism. Mol. Biol. Cell 14: 2116-2127 [Abstract] [Full Text]
Homman-Loudiyi, M., Hultenby, K., Britt, W., Soderberg-Naucler, C. (2003). Envelopment of Human Cytomegalovirus Occurs by Budding into Golgi-Derived Vacuole Compartments Positive for gB, Rab 3, Trans-Golgi Network 46, and Mannosidase II. J. Virol. 77: 3191-3203 [Abstract] [Full Text]
Chen, T.-l. L., Chen, C., Bergeron, N. Q., Close, B. E., Bohrer, T. J., Vertel, B. M., Colley, K. J. (2003). The two rat {alpha}2,6-sialyltransferase (ST6Gal I) isoforms: evaluation of catalytic activity and intra-Golgi localization. Glycobiology 13: 109-117 [Abstract] [Full Text]
Junn, E., Lee, S. S., Suhr, U. T., Mouradian, M. M. (2002). Parkin Accumulation in Aggresomes Due to Proteasome Impairment. J. Biol. Chem. 277: 47870-47877 [Abstract] [Full Text]
Szaszi, K., Paulsen, A., Szabo, E. Z., Numata, M., Grinstein, S., Orlowski, J. (2002). Clathrin-mediated Endocytosis and Recycling of the Neuron-specific Na+/H+ Exchanger NHE5 Isoform. REGULATION BY PHOSPHATIDYLINOSITOL 3'-KINASE AND THE ACTIN CYTOSKELETON. J. Biol. Chem. 277: 42623-42632 [Abstract] [Full Text]
Spiliotis, E. T., Pentcheva, T., Edidin, M. (2002). Probing for Membrane Domains in the Endoplasmic Reticulum: Retention and Degradation of Unassembled MHC Class I Molecules. Mol. Biol. Cell 13: 1566-1581 [Abstract] [Full Text]
Chen, X., Shen, J., Prywes, R. (2002). The Luminal Domain of ATF6 Senses Endoplasmic Reticulum (ER) Stress and Causes Translocation of ATF6 from the ER to the Golgi. J. Biol. Chem. 277: 13045-13052 [Abstract] [Full Text]
Bantel-Schaal, U., Hub, B., Kartenbeck, J. (2002). Endocytosis of Adeno-Associated Virus Type 5 Leads to Accumulation of Virus Particles in the Golgi Compartment. J. Virol. 76: 2340-2349 [Abstract] [Full Text]
Zerfaoui, M., Fukuda, M., Langlet, C., Mathieu, S., Suzuki, M., Lombardo, D., El-Battari, A. (2002). The cytosolic and transmembrane domains of the {beta}1,6 N-acetylglucosaminyltransferase (C2GnT) function as a cis to medial/Golgi-targeting determinant. Glycobiology 12: 15-24 [Abstract] [Full Text]
Bai, X., Zhou, D., Brown, J. R., Crawford, B. E., Hennet, T., Esko, J. D. (2001). Biosynthesis of the Linkage Region of Glycosaminoglycans. CLONING AND ACTIVITY OF GALACTOSYLTRANSFERASE II, THE SIXTH MEMBER OF THE beta 1,3-GALACTOSYLTRANSFERASE FAMILY (beta 3GalT6). J. Biol. Chem. 276: 48189-48195 [Abstract] [Full Text]
Pinhal, M. A. S., Smith, B., Olson, S., Aikawa, J.-i., Kimata, K., Esko, J. D. (2001). Enzyme interactions in heparan sulfate biosynthesis: Uronosyl 5-epimerase and 2-O-sulfotransferase interact in vivo. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241175798v1 [Abstract] [Full Text]
Nelson, C. S., Ikeda, M., Gompf, H. S., Robinson, M. L., Fuchs, N. K., Yoshioka, T., Neve, K. A., Allen, C. N. (2001). Regulation of Melatonin 1a Receptor Signaling and Trafficking by Asparagine-124. Mol. Endocrinol. 15: 1306-1317 [Abstract] [Full Text]
Waelter, S., Boeddrich, A., Lurz, R., Scherzinger, E., Lueder, G., Lehrach, H., Wanker, E. E. (2001). Accumulation of Mutant Huntingtin Fragments in Aggresome-like Inclusion Bodies as a Result of Insufficient Protein Degradation. Mol. Biol. Cell 12: 1393-1407 [Abstract] [Full Text]
Roseberry, A. G., Bünemann, M., Elavunkal, J., Hosey, M. M. (2001). Agonist-Dependent Delivery of M2 Muscarinic Acetylcholine Receptors to the Cell Surface after Pertussis Toxin Treatment. Mol. Pharmacol. 59: 1256-1268 [Abstract] [Full Text]
Ulloa, F., Real, F. X. (2001). Differential Distribution of Sialic Acid in {{alpha}}2,3 and {{alpha}}2,6 Linkages in the Apical Membrane of Cultured Epithelial Cells and Tissues. J. Histochem. Cytochem. 49: 501-510 [Abstract] [Full Text]
Sohn, H. W., Shin, Y. K., Lee, I.-S., Bae, Y. M., Suh, Y. H., Kim, M. K., Kim, T. J., Jung, K. C., Park, W. S., Park, C.-S., Chung, D. H., Ahn, K., Kim, I. S., Ko, Y. H., Bang, Y. J., Kim, C. W., Park, S. H. (2001). CD99 Regulates the Transport of MHC Class I Molecules from the Golgi Complex to the Cell Surface. J. Immunol. 166: 787-794 [Abstract] [Full Text]
Aridor, M., Fish, K. N., Bannykh, S., Weissman, J., Roberts, T. H., Lippincott-Schwartz, J., Balch, W. E. (2001). The Sar1 Gtpase Coordinates Biosynthetic Cargo Selection with Endoplasmic Reticulum Export Site Assembly. JCB 152: 213-230 [Abstract] [Full Text]
Zuber, C., Spiro, M. J., Guhl, B., Spiro, R. G., Roth, J. (2000). Golgi Apparatus Immunolocalization of Endomannosidase Suggests Post-Endoplasmic Reticulum Glucose Trimming: Implications for Quality Control. Mol. Biol. Cell 11: 4227-4240 [Abstract] [Full Text]
Orci, L., Amherdt, M., Ravazzola, M., Perrelet, A., Rothman, J. E. (2000). Exclusion of Golgi Residents from Transport Vesicles Budding from Golgi Cisternae in Intact Cells. JCB 150: 1263-1270 [Abstract] [Full Text]
Martin, M. E., Hidalgo, J., Rosa, J. L., Crottet, P., Velasco, A. (2000). Effect of Protein Kinase A Activity on the Association of ADP-ribosylation Factor 1 to Golgi Membranes. J. Biol. Chem. 275: 19050-19059 [Abstract] [Full Text]
Hoja-Lukowicz, D., Ciolczyk, D., Bergquist, J., Litynska, A., Laidler, P. (2000). High-mannose-type oligosaccharides from human placental arylsulfatase A are core fucosylated as confirmed by MALDI MS. Glycobiology 10: 551-557 [Abstract] [Full Text]
Corse, E., Machamer, C. E. (2000). Infectious Bronchitis Virus E Protein Is Targeted to the Golgi Complex and Directs Release of Virus-Like Particles. J. Virol. 74: 4319-4326 [Abstract] [Full Text]
Boehmelt, G., Fialka, I., Brothers, G., McGinley, M. D., Patterson, S. D., Mo, R., Hui, C. C., Chung, S., Huber, L. A., Mak, T. W., Iscove, N. N. (2000). Cloning and Characterization of the Murine Glucosamine-6-phosphate Acetyltransferase EMeg32. DIFFERENTIAL EXPRESSION AND INTRACELLULAR MEMBRANE ASSOCIATION. J. Biol. Chem. 275: 12821-12832 [Abstract] [Full Text]
Korimilli, A., Gonzales, L. W., Guttentag, S. H. (2000). Intracellular Localization of Processing Events in Human Surfactant Protein B Biosynthesis. J. Biol. Chem. 275: 8672-8679 [Abstract] [Full Text]
Pedrazzini, E., Villa, A., Longhi, R., Bulbarelli, A., Borgese, N. (2000). Mechanism of Residence of Cytochrome B(5), a Tail-Anchored Protein, in the Endoplasmic Reticulum. JCB 148: 899-914 [Abstract] [Full Text]
Zhao, Y., McCabe, J. B., Vance, J., Berthiaume, L. G. (2000). Palmitoylation of Apolipoprotein B Is Required for Proper Intracellular Sorting and Transport of Cholesteroyl Esters and Triglycerides. Mol. Biol. Cell 11: 721-734 [Abstract] [Full Text]
Ermonval, M., Duvet, S., Zonneveld, D., Cacan, R., Buttin, G., Braakman, I. (2000). Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding. Glycobiology 10: 77-87 [Abstract] [Full Text]
Brigance, W. T., Barlowe, C., Graham, T. R. (2000). Organization of the Yeast Golgi Complex into at Least Four Funtionally Distinct Compartments. Mol. Biol. Cell 11: 171-182 [Abstract] [Full Text]
Lin, C.-C., Love, H. D., Gushue, J. N., Bergeron, J. J.M., Ostermann, J. (1999). ER/Golgi Intermediates Acquire Golgi Enzymes by Brefeldin a-Sensitive Retrograde Transport in Vitro. JCB 147: 1457-1472 [Abstract] [Full Text]
Alvarez, C., Fujita, H., Hubbard, A., Sztul, E. (1999). ER to Golgi Transport: Requirement for P115 at a Pre-Golgi Vtc Stage. JCB 147: 1205-1222 [Abstract] [Full Text]
Cabrita, M. A., Hobman, T. C., Hogue, D. L., King, K. M., Cass, C. E. (1999). Mouse Transporter Protein, a Membrane Protein That Regulates Cellular Multidrug Resistance, Is Localized to Lysosomes. Cancer Res. 59: 4890-4897 [Abstract] [Full Text]
Cikaluk, D. E., Tahbaz, N., Hendricks, L. C., DiMattia, G. E., Hansen, D., Pilgrim, D., Hobman, T. C. (1999). GERp95, a Membrane-associated Protein that Belongs to a Family of Proteins Involved in Stem Cell Differentiation. Mol. Biol. Cell 10: 3357-3372 [Abstract] [Full Text]
Nohturfft, A., DeBose-Boyd, R. A., Scheek, S., Goldstein, J. L., Brown, M. S. (1999). Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi. Proc. Natl. Acad. Sci. USA 96: 11235-11240 [Abstract] [Full Text]
Gonzalez, D. S., Karaveg, K., Vandersall-Nairn, A. S., Lal, A., Moremen, K. W. (1999). Identification, Expression, and Characterization of a cDNA Encoding Human Endoplasmic Reticulum Mannosidase I, the Enzyme That Catalyzes the First Mannose Trimming Step in Mammalian Asn-linked Oligosaccharide Biosynthesis. J. Biol. Chem. 274: 21375-21386 [Abstract] [Full Text]
Nishimura, N., Bannykh, S., Slabough, S., Matteson, J., Altschuler, Y., Hahn, K., Balch, W. E. (1999). A Di-acidic (DXE) Code Directs Concentration of Cargo during Export from the Endoplasmic Reticulum. J. Biol. Chem. 274: 15937-15946 [Abstract] [Full Text]
Garbutt, M., Law, L. M. J., Chan, H., Hobman, T. C. (1999). Role of Rubella Virus Glycoprotein Domains in Assembly of Virus-Like Particles. J. Virol. 73: 3524-3533 [Abstract] [Full Text]
Lin, P., Yao, Y., Hofmeister, R., Tsien, R. Y., Farquhar, M. G. (1999). Overexpression of CALNUC (Nucleobindin) Increases Agonist and Thapsigargin Releasable Ca2+ Storage in the Golgi. JCB 145: 279-289 [Abstract] [Full Text]
Eckhardt, M., Gotza, B., Gerardy-Schahn, R. (1999). Membrane Topology of the Mammalian CMP-Sialic Acid Transporter. J. Biol. Chem. 274: 8779-8787 [Abstract] [Full Text]
Rabouille, C, Kuntz, D., Lockyer, A, Watson, R, Signorelli, T, Rose, D., van den Heuvel, M, Roberts, D. (1999). The Drosophila GMII gene encodes a Golgi alpha-mannosidase II. J. Cell Sci. 112: 3319-3330 [Abstract]
Rowe, J, Corradi, N, Malosio, M., Taverna, E, Halban, P, Meldolesi, J, Rosa, P (1999). Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells: prevention by rbSEC1. J. Cell Sci. 112: 1865-1877 [Abstract]
Ihida, K, Predescu, D, Czekay, R., Palade, G. (1999). Platelet activating factor receptor (PAF-R) is found in a large endosomal compartment in human umbilical vein endothelial cells. J. Cell Sci. 112: 285-295 [Abstract]
Johnston, J. A., Ward, C. L., Kopito, R. R. (1998). Aggresomes: A Cellular Response to Misfolded Proteins. JCB 143: 1883-1898 [Abstract] [Full Text]
De Vries, L., Lou, X., Zhao, G., Zheng, B., Farquhar, M. G. (1998). GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP. Proc. Natl. Acad. Sci. USA 95: 12340-12345 [Abstract] [Full Text]
Eckhardt, M., Gotza, B., Gerardy-Schahn, R. (1998). Mutants of the CMP-sialic Acid Transporter Causing the Lec2 Phenotype. J. Biol. Chem. 273: 20189-20195 [Abstract] [Full Text]
Tai, A. W., Chuang, J.-Z., Sung, C.-H. (1998). Localization of Tctex-1, a Cytoplasmic Dynein Light Chain, to the Golgi Apparatus and Evidence for Dynein Complex Heterogeneity. J. Biol. Chem. 273: 19639-19649 [Abstract] [Full Text]
Yan, D., Swain, P. K., Breuer, D., Tucker, R. M., Wu, W., Fujita, R., Rehemtulla, A., Burke, D., Swaroop, A. (1998). Biochemical Characterization and Subcellular Localization of the Mouse Retinitis Pigmentosa GTPase Regulator (mRpgr). J. Biol. Chem. 273: 19656-19663 [Abstract] [Full Text]
Lin, P., Le-Niculescu, H., Hofmeister, R., Michael McCaffery, J., Jin, M., Hennemann, H., McQuistan, T., De Vries, L., Farquhar, M. G. (1998). The Mammalian Calcium-binding Protein, Nucleobindin (CALNUC), Is a Golgi Resident Protein. JCB 141: 1515-1527 [Abstract] [Full Text]
Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G., Tsien, R. Y. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95: 6803-6808 [Abstract] [Full Text]
Hobman, T. C., Zhao, B., Chan, H., Farquhar, M. G. (1998). Immunoisolation and Characterization of a Subdomain of the Endoplasmic Reticulum That Concentrates Proteins Involved in COPII Vesicle Biogenesis. Mol. Biol. Cell 9: 1265-1278 [Abstract] [Full Text]
De Vries, L., Elenko, E., McCaffery, J. M., Fischer, T., Hubler, L., McQuistan, T., Watson, N., Farquhar, M. G. (1998). RGS-GAIP, a GTPase-activating Protein for Galpha i Heterotrimeric G Proteins, Is Located on Clathrin-coated Vesicles. Mol. Biol. Cell 9: 1123-1134 [Abstract] [Full Text]
Mironov, A. JR., Luini, A., Mironov, A. (1998). A synthetic model of intra-Golgi traffic. FASEB J. 12: 249-252 [Abstract] [Full Text]
Rowe, T., Dascher, C., Bannykh, S., Plutner, H., Balch, W. E. (1998). Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates. Science 279: 696-700 [Abstract] [Full Text]
D'Souza, S., Garcia-Cabado, A., Yu, F., Teter, K., Lukacs, G., Skorecki, K., Moore, H.-P., Orlowski, J., Grinstein, S. (1998). The Epithelial Sodium-Hydrogen Antiporter Na+/H+ Exchanger 3�Accumulates and Is Functional in Recycling Endosomes. J. Biol. Chem. 273: 2035-2043 [Abstract] [Full Text]
Porwoll, S., Loch, N., Kannicht, C., Nuck, R., Grunow, D., Reutter, W., Tauber, R. (1998). Cell Surface Glycoproteins Undergo Postbiosynthetic Modification of Their N-Glycans by Stepwise Demannosylation. J. Biol. Chem. 273: 1075-1085 [Abstract] [Full Text]
Zheng, J., Koda, T, Fujiwara, T, Kishi, M, Ikehara, Y, Kakinuma, M (1998). A novel Rab GTPase, Rab33B, is ubiquitously expressed and localized to the medial Golgi cisternae. J. Cell Sci. 111: 1061-1069 [Abstract]
Kukuruzinska, M.A., Lennon, K. (1998). Protein N-Glycosylation: Molecular Genetics and Functional Significance. CROBM 9: 415-448 [Abstract] [Full Text]
Rottger, S, White, J, Wandall, H., Olivo, J., Stark, A, Bennett, E., Whitehouse, C, Berger, E., Clausen, H, Nilsson, T (1998). Localization of three human polypeptide GalNAc-transferases in HeLa cells suggests initiation of O-linked glycosylation throughout the Golgi apparatus. J. Cell Sci. 111: 45-60 [Abstract]
Muniz, M., Martin, M. E., Hidalgo, J., Velasco, A. (1997). Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi�network. Proc. Natl. Acad. Sci. USA 94: 14461-14466 [Abstract] [Full Text]
Maceyka, M., Machamer, C. E. (1997). Ceramide Accumulation Uncovers a Cycling Pathway for the cis-Golgi Network Marker, Infectious Bronchitis Virus M Protein. JCB 139: 1411-1418 [Abstract] [Full Text]
Deschenes, S. M., Walcott, J. L., Wexler, T. L., Scherer, S. S., Fischbeck, K. H. (1997). Altered Trafficking of Mutant Connexin32. J. Neurosci. 17: 9077-9084 [Abstract] [Full Text]
Fath, K. R., Trimbur, G. M., Burgess, D. R. (1997). Molecular Motors and a Spectrin Matrix Associate with Golgi Membranes In Vitro. JCB 139: 1169-1181 [Abstract] [Full Text]
Rolls, M. M., Marquardt, M. T., Kielian, M., Machamer, C. E. (1997). Cholesterol-independent Targeting of Golgi Membrane Proteins in Insect Cells. Mol. Biol. Cell 8: 2111-2118 [Abstract] [Full Text]
Cluett, E. B., Kuismanen, E., Machamer, C. E. (1997). Heterogeneous Distribution of the Unusual Phospholipid Semilysobisphosphatidic Acid through the Golgi Complex. Mol. Biol. Cell 8: 2233-2240 [Abstract] [Full Text]
Yang, L., Guan, T., Gerace, L. (1997). Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis. JCB 137: 1199-1210 [Abstract] [Full Text]
Ojakian, G., Nelson, W., Beck, K. (1997). Mechanisms for de novo biogenesis of an apical membrane compartment in groups of simple epithelial cells surrounded by extracellular matrix. J. Cell Sci. 110: 2781-2794 [Abstract]
Muniz, M., Alonso, M., Hidalgo, J., Velasco, A. (1996). A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route. J. Biol. Chem. 271: 30935-30941 [Abstract] [Full Text]
Rooney, E., Meldolesi, J. (1996). The Endoplasmic Reticulum in PC12 Cells. EVIDENCE FOR A MOSAIC OF DOMAINS DIFFERENTLY SPECIALIZED IN Ca2+ HANDLING. J. Biol. Chem. 271: 29304-29311 [Abstract] [Full Text]
Tisdale, E. J., Balch, W. E. (1996). Rab2 Is Essential for the Maturation of Pre-Golgi Intermediates. J. Biol. Chem. 271: 29372-29379 [Abstract] [Full Text]
Bock, J. B., Lin, R. C., Scheller, R. H. (1996). A New Syntaxin Family Member Implicated in Targeting of Intracellular Transport Vesicles. J. Biol. Chem. 271: 17961-17965 [Abstract] [Full Text]
Levine, T. P., Rabouille, C., Kieckbusch, R. H., Warren, G. (1996). Binding of the Vesicle Docking Protein p115 to Golgi Membranes Is Inhibited under Mitotic Conditions. J. Biol. Chem. 271: 17304-17311 [Abstract] [Full Text]
Perez-Vilar, J., Eckhardt, A. E., Hill, R. L. (1996). Porcine Submaxillary Mucin Forms Disulfide-bonded Dimers between Its Carboxyl-terminal Domains. J. Biol. Chem. 271: 9845-9850 [Abstract] [Full Text]
Ma, J., Colley, K. J. (1996). A Disulfide-bonded Dimer of the Golgi beta-Galactoside alpha2,6-Sialyltransferase Is Catalytically Inactive yet Still Retains the Ability to Bind Galactose. J. Biol. Chem. 271: 7758-7766 [Abstract] [Full Text]
Wang, C.-N., Hobman, T. C., Brindley, D. N. (1995). Degradation of Apolipoprotein B in Cultured Rat Hepatocytes Occurs in a Post-endoplasmic Reticulum Compartment. J. Biol. Chem. 270: 24924-24931 [Abstract] [Full Text]
Hoe, M. H., Slusarewicz, P., Misteli, T., Watson, R., Warren, G. (1995). Evidence for Recycling of the Resident medial/trans Golgi Enzyme, N-Acetylglucosaminyltransferase I, in ldlD Cells. J. Biol. Chem. 270: 25057-25063 [Abstract] [Full Text]
Leung, H. T., Bradshaw, J., Cleaveland, J. S., Linsley, P. S. (1995). Cytotoxic T Lymphocyte-associated Molecule-4, a High Avidity Receptor for CD80 and CD86, Contains an Intracellular Localization Motif in Its Cytoplasmic Tail. J. Biol. Chem. 270: 25107-25114 [Abstract] [Full Text]
(1995). . J. Biol. Chem. 270: 15211-15221 [Abstract] [Full Text]
Etchison, J. R., Srikrishna, G., Freeze, H. H. (1995). A Novel Method to Co-localize Glycosaminoglycan-Core Oligosaccharide Glycosyltransferases in Rat Liver Golgi. J. Biol. Chem. 270: 756-764 [Abstract] [Full Text]
Rabouille, C, Hui, N, Hunte, F, Kieckbusch, R, Berger, E., Warren, G, Nilsson, T (1995). Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108: 1617-1627 [Abstract]
Hidalgo, J, Muniz, M, Velasco, A (1995). Trimeric G proteins regulate the cytosol-induced redistribution of Golgi enzymes into the endoplasmic reticulum. J. Cell Sci. 108: 1805-1815 [Abstract]
Horvat, R, Palade, G. (1995). The functional thrombin receptor is associated with the plasmalemma and a large endosomal network in cultured human umbilical vein endothelial cells. J. Cell Sci. 108: 1155-1164 [Abstract]
Acharya, U., Malhotra, V. (1995). Reconstitution of Golgi Stacks from Vesiculated Golgi Membranes in Permeabilized Cells. Cold Spring Harb Symp Quant Biol 60: 559-566 [Abstract]
Suh, W., Cohen-Doyle, M., Fruh, K, Wang, K, Peterson, P., Williams, D. (1994). Interaction of MHC class I molecules with the transporter associated with antigen processing. Science 264: 1322-1326 [Abstract]
Jackson, M., Cohen-Doyle, M., Peterson, P., Williams, D. (1994). Regulation of MHC class I transport by the molecular chaperone, calnexin (p88, IP90). Science 263: 384-387 [Abstract]
Pryde, J. (1994). A group of integral membrane proteins of the rat liver Golgi contains a conserved protein of 100 kDa. J. Cell Sci. 107: 3425-3436 [Abstract]
Johnston, P., Stieber, A, Gonatas, N. (1994). A hypothesis on the traffic of MG160, a medial Golgi sialoglycoprotein, from the trans-Golgi network to the Golgi cisternae. J. Cell Sci. 107: 529-537 [Abstract]