Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae

doi:10.1083/jcb.122.2.307

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Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae

C Abeijon, K Yanagisawa, EC Mandon, A Hausler, K Moremen, CB Hirschberg and PW Robbins
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.

Current models for nucleotide sugar use in the Golgi apparatus predict a critical role for the lumenal nucleoside diphosphatase. After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol. To test this model, we cloned the gene for the S. cerevisiae guanosine diphosphatase and constructed a null mutation. This mutation should reduce the concentrations of GDP-mannose and GMP and increase the concentration of GDP in the Golgi lumen. The alterations should in turn decrease mannosylation of proteins and lipids in this compartment. In fact, we found a partial block in O- and N-glycosylation of proteins such as chitinase and carboxypeptidase Y and underglycosylation of invertase. In addition, mannosylinositolphosphorylceramide levels were drastically reduced.
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Dunkley, T. P. J., Watson, R., Griffin, J. L., Dupree, P., Lilley, K. S. (2004). Localization of Organelle Proteins by Isotope Tagging (LOPIT). Mol. Cell. Proteomics 3: 1128-1134 [Abstract] [Full Text]
Uccelletti, D., O'Callaghan, C., Berninsone, P., Zemtseva, I., Abeijon, C., Hirschberg, C. B. (2004). ire-1-dependent Transcriptional Up-regulation of a Lumenal Uridine Diphosphatase from Caenorhabditis elegans. J. Biol. Chem. 279: 27390-27398 [Abstract] [Full Text]
Zhong, X., Malhotra, R., Guidotti, G. (2003). ATP Uptake in the Golgi and Extracellular Release Require Mcd4 Protein and the Vacuolar H+-ATPase. J. Biol. Chem. 278: 33436-33444 [Abstract] [Full Text]
Yoko-o, T., Wiggins, C. A.R., Stolz, J., Peak-Chew, S. Y., Munro, S. (2003). An N-acetylglucosaminyltransferase of the Golgi apparatus of the yeast Saccharomyces cerevisiae that can modify N-linked glycans. Glycobiology 13: 581-589 [Abstract] [Full Text]
D'Alessio, C., Trombetta, E. S., Parodi, A. J. (2003). Nucleoside Diphosphatase and Glycosyltransferase Activities Can Localize to Different Subcellular Compartments in Schizosaccharomyces pombe. J. Biol. Chem. 278: 22379-22387 [Abstract] [Full Text]
Failer, B. U., Braun, N., Zimmermann, H. (2002). Cloning, Expression, and Functional Characterization of a Ca2+-dependent Endoplasmic Reticulum Nucleoside Diphosphatase. J. Biol. Chem. 277: 36978-36986 [Abstract] [Full Text]
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Recio, J. A., Páez, J. G., Maskeri, B., Loveland, M., Velasco, J. A., Notario, V. (2000). Both Normal and Transforming PCPH Proteins Have Guanosine Diphosphatase Activity But Only the Oncoprotein Cooperates with Ras in Activating Extracellular Signal-regulated Kinase ERK1. Cancer Res. 60: 1720-1728 [Abstract] [Full Text]
Zhong, X., Guidotti, G. (1999). A Yeast Golgi E-type ATPase with an Unusual Membrane Topology. J. Biol. Chem. 274: 32704-32711 [Abstract] [Full Text]
Gao, X.-D., Kaigorodov, V., Jigami, Y. (1999). YND1, a Homologue of GDA1, Encodes Membrane-bound Apyrase Required for Golgi N- and O-Glycosylation in Saccharomyces cerevisiae. J. Biol. Chem. 274: 21450-21456 [Abstract] [Full Text]
Puglielli, L., Mandon, E. C., Rancour, D. M., Menon, A. K., Hirschberg, C. B. (1999). Identification and Purification of the Rat Liver Golgi Membrane UDP-N-acetylgalactosamine Transporter. J. Biol. Chem. 274: 4474-4479 [Abstract] [Full Text]
Reggiori, F., Conzelmann, A. (1998). Biosynthesis of Inositol Phosphoceramides and Remodeling of Glycosylphosphatidylinositol Anchors in Saccharomyces cerevisiae Are Mediated by Different Enzymes. J. Biol. Chem. 273: 30550-30559 [Abstract] [Full Text]
Valenzuela, J. G., Charlab, R., Galperin, M. Y., Ribeiro, J. M.�C. (1998). Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius. A NEW TYPE OF NUCLEOTIDE-BINDING ENZYME. J. Biol. Chem. 273: 30583-30590 [Abstract] [Full Text]
Beeler, T., Bacikova, D., Gable, K., Hopkins, L., Johnson, C., Slife, H., Dunn, T. (1998). The Saccharomyces cerevisiae TSC10/YBR265w Gene Encoding 3-Ketosphinganine Reductase Is Identified in a Screen for Temperature-sensitive Suppressors of the Ca2+-sensitive csg2Delta Mutant. J. Biol. Chem. 273: 30688-30694 [Abstract] [Full Text]
Abeijon, C., Chen, L. Y. (1998). The Role of Glucosidase I (Cwh41p) in the Biosynthesis of Cell Wall beta -1,6-Glucan Is Indirect. Mol. Biol. Cell 9: 2729-2738 [Abstract] [Full Text]
Wang, T.-F., Ou, Y., Guidotti, G. (1998). The Transmembrane Domains of Ectoapyrase (CD39) Affect Its Enzymatic Activity and Quaternary Structure. J. Biol. Chem. 273: 24814-24821 [Abstract] [Full Text]
Guillen, E., Abeijon, C., Hirschberg, C. B. (1998). Mammalian Golgi apparatus UDP-N-acetylglucosamine transporter: Molecular cloning by phenotypic correction of a yeast mutant. Proc. Natl. Acad. Sci. USA 95: 7888-7892 [Abstract] [Full Text]
Neckelmann, G., Orellana, A. (1998). Metabolism of Uridine 5'-Diphosphate-Glucose in Golgi Vesicles from Pea Stems. Plant Physiol. 117: 1007-1014 [Abstract] [Full Text]
Vowels, J. J., Payne, G. S. (1998). A Role for the Lumenal Domain in Golgi Localization of the Saccharomyces cerevisiae Guanosine Diphosphatase. Mol. Biol. Cell 9: 1351-1365 [Abstract] [Full Text]
Wang, T.-F., Guidotti, G. (1998). Golgi Localization and Functional Expression of Human Uridine Diphosphatase. J. Biol. Chem. 273: 11392-11399 [Abstract] [Full Text]
Wickert, S., Finck, M., Herz, B., Ernst, J. F. (1998). A Small Protein (Ags1p) and the Pho80p-Pho85p Kinase Complex Contribute to Aminoglycoside Antibiotic Resistance of the Yeast Saccharomyces cerevisiae. J. Bacteriol. 180: 1887-1894 [Abstract] [Full Text]
Kukuruzinska, M.A., Lennon, K. (1998). Protein N-Glycosylation: Molecular Genetics and Functional Significance. CROBM 9: 415-448 [Abstract] [Full Text]
Dean, N., Zhang, Y. B., Poster, J. B. (1997). The VRG4 Gene Is Required for GDP-mannose Transport into the Lumen of the Golgi in the Yeast, Saccharomyces cerevisiae. J. Biol. Chem. 272: 31908-31914 [Abstract] [Full Text]
Hashimoto, H., Sakakibara, A., Yamasaki, M., Yoda, K. (1997). Saccharomyces cerevisiae VIG9 Encodes GDP-mannose Pyrophosphorylase, Which Is Essential for Protein Glycosylation. J. Biol. Chem. 272: 16308-16314 [Abstract] [Full Text]
Ma, D., Russell, D. G., Beverley, S. M., Turco, S. J. (1997). Golgi GDP-mannose Uptake Requires Leishmania LPG2. A MEMBER OF A EUKARYOTIC FAMILY OF PUTATIVE NUCLEOTIDE-SUGAR TRANSPORTERS. J. Biol. Chem. 272: 3799-3805 [Abstract] [Full Text]
Smith, V., Chou, K. N., Lashkari, D., Botstein, D., Brown, P. O. (1996). Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting. Science 274: 2069-2074 [Abstract] [Full Text]
Vasconcelos, E. G., Ferreira, S. T., Carvalho, T. M.U.d., Souza, W. d., Kettlun, A. M., Mancilla, M., Valenzuela, M. A., Verjovski-Almeida, S. (1996). Partial Purification and Immunohistochemical Localization of ATP Diphosphohydrolase from Schistosoma mansoni. IMMUNOLOGICAL CROSS-REACTIVITIES WITH POTATO APYRASE AND TOXOPLASMA GONDII NUCLEOSIDE TRIPHOSPHATE HYDROLASE. J. Biol. Chem. 271: 22139-22145 [Abstract] [Full Text]
Wang, T.-F., Guidotti, G. (1996). CD39 Is an Ecto-(Ca[IMAGE],Mg[IMAGE])-apyrase. J. Biol. Chem. 271: 9898-9901 [Abstract] [Full Text]
Abeijon, C., Mandon, E. C., Robbins, P. W., Hirschberg, C. B. (1996). A Mutant Yeast Deficient in Golgi Transport of Uridine Diphosphate N-Acetylglucosamine. J. Biol. Chem. 271: 8851-8854 [Abstract] [Full Text]
Sipos, G.�r., Puoti, A., Conzelmann, A. (1995). Biosynthesis of the Side Chain of Yeast Glycosylphosphatidylinositol Anchors Is Operated by Novel Mannosyltransferases Located in the Endoplasmic Reticulum and the Golgi Apparatus. J. Biol. Chem. 270: 19709-19715 [Abstract] [Full Text]
Berninsone, P., Lin, Z.-Y., Kempner, E., Hirschberg, C. B. (1995). Regulation of Yeast Golgi Glycosylation. J. Biol. Chem. 270: 14564-14567 [Abstract] [Full Text]
Schwientek, T., Lorenz, C., Ernst, J. F. (1995). Golgi Localization in Yeast Is Mediated by the Membrane Anchor Region of Rat Liver Sialyltransferase. J. Biol. Chem. 270: 5483-5489 [Abstract] [Full Text]
Biederbick, A., Kosan, C., Kunz, J., Elsasser, H.-P. (2000). First Apyrase Splice Variants Have Different Enzymatic Properties. J. Biol. Chem. 275: 19018-19024 [Abstract] [Full Text]
Hicks-Berger, C. A., Chadwick, B. P., Frischauf, A.-M., Kirley, T. L. (2000). Expression and Characterization of Soluble and Membrane-bound Human Nucleoside Triphosphate Diphosphohydrolase 6 (CD39L2). J. Biol. Chem. 275: 34041-34045 [Abstract] [Full Text]
Zhong, X., Malhotra, R., Guidotti, G. (2000). Regulation of Yeast Ectoapyrase Ynd1p Activity by Activator Subunit Vma13p of Vacuolar H+-ATPase. J. Biol. Chem. 275: 35592-35599 [Abstract] [Full Text]
Zhong, X., Malhotra, R., Woodruff, R., Guidotti, G. (2001). Mammalian Plasma Membrane Ecto-nucleoside Triphosphate Diphosphohydrolase 1, CD39, Is Not Active Intracellularly. THE N-GLYCOSYLATION STATE OF CD39 CORRELATES WITH SURFACE ACTIVITY AND LOCALIZATION. J. Biol. Chem. 276: 41518-41525 [Abstract] [Full Text]
Berninsone, P., Hwang, H.-Y., Zemtseva, I., Horvitz, H. R., Hirschberg, C. B. (2001). SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose. Proc. Natl. Acad. Sci. USA 98: 3738-3743 [Abstract] [Full Text]