The Journal of Cell Biology, Vol 122, 789-807, Copyright © 1993 by The Rockefeller University Press

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Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells

M Sargiacomo, M Sudol, Z Tang and MP Lisanti
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142-1479.

GPI-linked protein molecules become Triton-insoluble during polarized sorting to the apical cell surface of epithelial cells. These insoluble complexes, enriched in cholesterol, glycolipids, and GPI-linked proteins, have been isolated by flotation on sucrose density gradients and are thought to contain the putative GPI-sorting machinery. As the cellular origin and molecular protein components of this complex remain unknown, we have begun to characterize these low-density insoluble complexes isolated from MDCK cells. We find that these complexes, which represent 0.4-0.8% of the plasma membrane, ultrastructurally resemble caveolae and are over 150-fold enriched in a model GPI-anchored protein and caveolin, a caveolar marker protein. However, they exclude many other plasma membrane associated molecules and organelle-specific marker enzymes, suggesting that they represent microdomains of the plasma membrane. In addition to caveolin, these insoluble complexes contain a subset of hydrophobic plasma membrane proteins and cytoplasmically-oriented signaling molecules, including: (a) GTP- binding proteins--both small and heterotrimeric; (b) annex II--an apical calcium-regulated phospholipid binding protein with a demonstrated role in exocytic fusion events; (c) c-Yes--an apically localized member of the Src family of non-receptor type protein- tyrosine kinases; and (d) an unidentified serine-kinase activity. As we demonstrate that caveolin is both a transmembrane molecule and a major phospho-acceptor component of these complexes, we propose that caveolin could function as a transmembrane adaptor molecule that couples luminal GPI-linked proteins with cytoplasmically oriented signaling molecules during GPI-membrane trafficking or GPI-mediated signal transduction events. In addition, our results have implications for understanding v- Src transformation and the actions of cholera and pertussis toxins on hetero-trimeric G proteins.
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• Garver, W. S., Krishnan, K., Gallagos, J. R., Michikawa, M., Francis, G. A., Heidenreich, R. A. (2002). Niemann-Pick C1 protein regulates cholesterol transport to the trans-Golgi network and plasma membrane caveolae. J. Lipid Res. 43: 579-589 [Abstract] [Full Text]  
• Razani, B., Wang, X. B., Engelman, J. A., Battista, M., Lagaud, G., Zhang, X. L., Kneitz, B., Hou, H. Jr., Christ, G. J., Edelmann, W., Lisanti, M. P. (2002). Caveolin-2-Deficient Mice Show Evidence of Severe Pulmonary Dysfunction without Disruption of Caveolae. Mol. Cell. Biol. 22: 2329-2344 [Abstract] [Full Text]  
• Zhuang, L., Lin, J., Lu, M. L., Solomon, K. R., Freeman, M. R. (2002). Cholesterol-rich Lipid Rafts Mediate Akt-regulated Survival in Prostate Cancer Cells. Cancer Res. 62: 2227-2231 [Abstract] [Full Text]  
• Chen, Y.-H., Lu, Q., Goodenough, D. A., Jeansonne, B. (2002). Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells. Mol. Biol. Cell 13: 1227-1237 [Abstract] [Full Text]  
• Vargas, L., Nore, B. F., Berglof, A., Heinonen, J. E., Mattsson, P. T., Smith, C. I. E., Mohamed, A. J. (2002). Functional Interaction of Caveolin-1 with Bruton's Tyrosine Kinase and Bmx. J. Biol. Chem. 277: 9351-9357 [Abstract] [Full Text]  
• Breuza, L., Corby, S., Arsanto, J.-P., Delgrossi, M.-H., Scheiffele, P., Le Bivic, A. (2002). The scaffolding domain of caveolin 2 is responsible for its Golgi localization in Caco-2 cells. J. Cell Sci. 115: 4457-4467 [Abstract] [Full Text]  
• Hakomori, S.-i. (2002). The glycosynapse. Proc. Natl. Acad. Sci. USA 10.1073/pnas.012540899v1 [Abstract] [Full Text]  
• Razani, B., Lisanti, M. P. (2001). Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein kinase A. Am. J. Physiol. Cell Physiol. 281: C1241-C1250 [Abstract] [Full Text]  
• Fujita, T., Toya, Y., Iwatsubo, K., Onda, T., Kimura, K., Umemura, S., Ishikawa, Y. (2001). Accumulation of molecules involved in {alpha}1-adrenergic signal within caveolae: caveolin expression and the development of cardiac hypertrophy. Cardiovasc Res 51: 709-716 [Abstract] [Full Text]  
• Kimura, A., Baumann, C. A., Chiang, S.-H., Saltiel, A. R. (2001). The sorbin homology domain: A motif for the targeting of proteins to lipid rafts. Proc. Natl. Acad. Sci. USA 98: 9098-9103 [Abstract] [Full Text]  
• Muller, G., Jung, C., Wied, S., Welte, S., Jordan, H., Frick, W. (2001). Redistribution of Glycolipid Raft Domain Components Induces Insulin-Mimetic Signaling in Rat Adipocytes. Mol. Cell. Biol. 21: 4553-4567 [Abstract] [Full Text]  
• Gkantiragas, I., Brugger, B., Stuven, E., Kaloyanova, D., Li, X.-Y., Lohr, K., Lottspeich, F., Wieland, F. T., Helms, J. B. (2001). Sphingomyelin-enriched Microdomains at the Golgi Complex. Mol. Biol. Cell 12: 1819-1833 [Abstract] [Full Text]  
• Suzuki, T., Kiyokawa, N., Taguchi, T., Sekino, T., Katagiri, Y. U., Fujimoto, J. (2001). CD24 Induces Apoptosis in Human B Cells Via the Glycolipid-Enriched Membrane Domains/Rafts-Mediated Signaling System. J. Immunol. 166: 5567-5577 [Abstract] [Full Text]  
• Ginés, S., Ciruela, F., Burgueño, J., Casadó, V., Canela, Enric. I., Mallol, J., Lluís, C., Franco, R. (2001). Involvement of Caveolin in Ligand-Induced Recruitment and Internalization of A1 Adenosine Receptor and Adenosine Deaminase in an Epithelial Cell Line. Mol. Pharmacol. 59: 1314-1323 [Abstract] [Full Text]  
• Liu, J., Lee, P., Galbiati, F., Kitsis, R. N., Lisanti, M. P. (2001). Caveolin-1 expression sensitizes fibroblastic and epithelial cells to apoptotic stimulation. Am. J. Physiol. Cell Physiol. 280: C823-C835 [Abstract] [Full Text]  
• Nusrat, A., von Eichel-Streiber, C., Turner, J. R., Verkade, P., Madara, J. L., Parkos, C. A. (2001). Clostridium difficile Toxins Disrupt Epithelial Barrier Function by Altering Membrane Microdomain Localization of Tight Junction Proteins. Infect. Immun. 69: 1329-1336 [Abstract] [Full Text]  
• Oh, P., Schnitzer, J. E. (2001). Segregation of Heterotrimeric G Proteins in Cell Surface Microdomains. Gq Binds Caveolin to Concentrate in Caveolae, whereas Gi and Gs Target Lipid Rafts by Default. Mol. Biol. Cell 12: 685-698 [Abstract] [Full Text]  
• Li, H., Brodsky, S., Basco, M., Romanov, V., De Angelis, D. A., Goligorsky, M. S. (2001). Nitric Oxide Attenuates Signal Transduction : Possible Role in Dissociating Caveolin-1 Scaffold. Circ. Res. 88: 229-236 [Abstract] [Full Text]  
• Felley-Bosco, E., Bender, F. C., Courjault-Gautier, F., Bron, C., Quest, A. F. G. (2000). Caveolin-1 down-regulates inducible nitric oxide synthase via the proteasome pathway in human colon carcinoma cells. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250406797v1 [Abstract] [Full Text]  

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