A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin

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A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin

JM Ervasti and KP Campbell
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242.

The dystrophin-glycoprotein complex was tested for interaction with several components of the extracellular matrix as well as actin. The 156-kD dystrophin-associated glycoprotein (156-kD dystroglycan) specifically bound laminin in a calcium-dependent manner and was inhibited by NaCl (IC50 = 250 mM) but was not affected by 1,000-fold (wt/wt) excesses of lactose, IKVAV, or YIGSR peptides. Laminin binding was inhibited by heparin (IC50 = 100 micrograms/ml), suggesting that one of the heparin-binding domains of laminin is involved in binding dystroglycan while negatively charged oligosaccharide moieties on dystroglycan were found to be necessary for its laminin-binding activity. No interaction between any component of the dystrophin- glycoprotein complex and fibronectin, collagen I, collagen IV, entactin, or heparan sulfate proteoglycan was detected by 125I-protein overlay and/or extracellular matrix protein-Sepharose precipitation. In addition, laminin-Sepharose quantitatively precipitated purified dystrophin-glycoprotein complex, demonstrating that the laminin-binding site is accessible when dystroglycan is associated with the complex. Dystroglycan of nonmuscle tissues also bound laminin. However, the other proteins of the striated muscle dystrophin-glycoprotein complex appear to be absent, antigenically dissimilar or less tightly associated with dystroglycan in nonmuscle tissues. Finally, we show that the dystrophin-glycoprotein complex cosediments with F-actin but does not bind calcium or calmodulin. Our results support a role for the striated muscle dystrophin-glycoprotein complex in linking the actin- based cytoskeleton with the extracellular matrix. Furthermore, our results suggest that dystrophin and dystroglycan may play substantially different functional roles in nonmuscle tissues.
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Yamamoto, L. U., Velloso, F. J., Lima, B. L., Fogaca, L. L.Q., de Paula, F., Vieira, N. M., Zatz, M., Vainzof, M. (2008). Muscle Protein Alterations in LGMD2I Patients With Different Mutations in the Fukutin-related Protein Gene. J. Histochem. Cytochem. 56: 995-1001 [Abstract] [Full Text]
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Hijikata, T., Nakamura, A., Isokawa, K., Imamura, M., Yuasa, K., Ishikawa, R., Kohama, K., Takeda, S., Yorifuji, H. (2008). Plectin 1 links intermediate filaments to costameric sarcolemma through {beta}-synemin, {alpha}-dystrobrevin and actin. J. Cell Sci. 121: 2062-2074 [Abstract] [Full Text]
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Zhang, J., Bang, M.-L., Gokhin, D. S., Lu, Y., Cui, L., Li, X., Gu, Y., Dalton, N. D., Scimia, M. C., Peterson, K. L., Lieber, R. L., Chen, J. (2008). Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture. Am. J. Physiol. Cell Physiol. 294: C1175-C1182 [Abstract] [Full Text]
Kizuka, Y., Kobayashi, K., Kakuda, S., Nakajima, Y., Itoh, S., Kawasaki, N., Oka, S. (2008). Laminin-1 is a novel carrier glycoprotein for the nonsulfated HNK-1 epitope in mouse kidney. Glycobiology 18: 331-338 [Abstract] [Full Text]
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Rojek, J. M., Perez, M., Kunz, S. (2008). Cellular Entry of Lymphocytic Choriomeningitis Virus. J. Virol. 82: 1505-1517 [Abstract] [Full Text]
Akhavan, A., Crivelli, S. N., Singh, M., Lingappa, V. R., Muschler, J. L. (2008). SEA domain proteolysis determines the functional composition of dystroglycan. FASEB J. 22: 612-621 [Abstract] [Full Text]
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Banks, G. B., Gregorevic, P., Allen, J. M., Finn, E. E., Chamberlain, J. S. (2007). Functional capacity of dystrophins carrying deletions in the N-terminal actin-binding domain. Hum Mol Genet 16: 2105-2113 [Abstract] [Full Text]
Dye, W. W., Gleason, R. L., Wilson, E., Humphrey, J. D. (2007). Altered biomechanical properties of carotid arteries in two mouse models of muscular dystrophy. J. Appl. Physiol. 103: 664-672 [Abstract] [Full Text]
Chamberlain, J. S., Metzger, J., Reyes, M., Townsend, D., Faulkner, J. A. (2007). Dystrophin-deficient mdx mice display a reduced life span and are susceptible to spontaneous rhabdomyosarcoma. FASEB J. 21: 2195-2204 [Abstract] [Full Text]
Xu, R., Chandrasekharan, K., Yoon, J. H., Camboni, M., Martin, P. T. (2007). Overexpression of the Cytotoxic T Cell (CT) Carbohydrate Inhibits Muscular Dystrophy in the dyW Mouse Model of Congenital Muscular Dystrophy 1A. Am. J. Pathol. 171: 181-199 [Abstract] [Full Text]
Rojek, J. M., Spiropoulou, C. F., Campbell, K. P., Kunz, S. (2007). Old World and Clade C New World Arenaviruses Mimic the Molecular Mechanism of Receptor Recognition Used by {alpha}-Dystroglycan's Host-Derived Ligands. J. Virol. 81: 5685-5695 [Abstract] [Full Text]
Peter, A. K., Miller, G., Crosbie, R. H. (2007). Disrupted mechanical stability of the dystrophin-glycoprotein complex causes severe muscular dystrophy in sarcospan transgenic mice. J. Cell Sci. 120: 996-1008 [Abstract] [Full Text]
Brown, R. H. Jr., Grant, P. E., Pierson, C. R. (2006). Case 35-2006 -- A Newborn Boy with Hypotonia. NEJM 355: 2132-2142 [Full Text]
Rice, K. M., Preston, D. L., Neff, D., Norton, M., Blough, E. R. (2006). Age-Related Dystrophin-Glycoprotein Complex Structure and Function in the Rat Extensor Digitorum Longus and Soleus Muscle. Journals of Gerontology Series A: Biological Sciences and Medical Sciences 61: 1119-1129 [Abstract] [Full Text]
Weir, M. L., Oppizzi, M. L., Henry, M. D., Onishi, A., Campbell, K. P., Bissell, M. J., Muschler, J. L. (2006). Dystroglycan loss disrupts polarity and {beta}-casein induction in mammary epithelial cells by perturbing laminin anchoring. J. Cell Sci. 119: 4047-4058 [Abstract] [Full Text]
Driss, A., Charrier, L., Yan, Y., Nduati, V., Sitaraman, S., Merlin, D. (2006). Dystroglycan receptor is involved in integrin activation in intestinal epithelia. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G1228-G1242 [Abstract] [Full Text]
Tremblay, M. R., Carbonetto, S. (2006). An Extracellular Pathway for Dystroglycan Function in Acetylcholine Receptor Aggregation and Laminin Deposition in Skeletal Myotubes. J. Biol. Chem. 281: 13365-13373 [Abstract] [Full Text]
Judge, L. M., Haraguchiln, M., Chamberlain, J. S. (2006). Dissecting the signaling and mechanical functions of the dystrophin-glycoprotein complex. J. Cell Sci. 119: 1537-1546 [Abstract] [Full Text]
Guo, C., Willem, M., Werner, A., Raivich, G., Emerson, M., Neyses, L., Mayer, U. (2006). Absence of {alpha}7 integrin in dystrophin-deficient mice causes a myopathy similar to Duchenne muscular dystrophy. Hum Mol Genet 15: 989-998 [Abstract] [Full Text]
Shah, W. A., Peng, H., Carbonetto, S. (2006). Role of non-raft cholesterol in lymphocytic choriomeningitis virus infection via {alpha}-dystroglycan.. J. Gen. Virol. 87: 673-678 [Abstract] [Full Text]
Barresi, R., Campbell, K. P. (2006). Dystroglycan: from biosynthesis to pathogenesis of human disease. J. Cell Sci. 119: 199-207 [Abstract] [Full Text]
Jastrow, H., Koulen, P., Altrock, W. D., Kroger, S. (2006). Identification of a {beta}-Dystroglycan Immunoreactive Subcompartment in Photoreceptor Terminals. IOVS 47: 17-24 [Abstract] [Full Text]
Zolkiewska, A. (2005). Ecto-ADP-ribose Transferases: Cell-Surface Response to Local Tissue Injury. Physiology 20: 374-381 [Abstract] [Full Text]
Kunz, S., Rojek, J. M., Kanagawa, M., Spiropoulou, C. F., Barresi, R., Campbell, K. P., Oldstone, M. B. A. (2005). Posttranslational Modification of {alpha}-Dystroglycan, the Cellular Receptor for Arenaviruses, by the Glycosyltransferase LARGE Is Critical for Virus Binding. J. Virol. 79: 14282-14296 [Abstract] [Full Text]
Imperiali, M., Thoma, C., Pavoni, E., Brancaccio, A., Callewaert, N., Oxenius, A. (2005). O Mannosylation of {alpha}-Dystroglycan Is Essential for Lymphocytic Choriomeningitis Virus Receptor Function. J. Virol. 79: 14297-14308 [Abstract] [Full Text]
Occhi, S., Zambroni, D., Del Carro, U., Amadio, S., Sirkowski, E. E., Scherer, S. S., Campbell, K. P., Moore, S. A., Chen, Z.-L., Strickland, S., Di Muzio, A., Uncini, A., Wrabetz, L., Feltri, M. L. (2005). Both Laminin and Schwann Cell Dystroglycan Are Necessary for Proper Clustering of Sodium Channels at Nodes of Ranvier. J. Neurosci. 25: 9418-9427 [Abstract] [Full Text]
Nerbonne, J. M., Kass, R. S. (2005). Molecular Physiology of Cardiac Repolarization. Physiol. Rev. 85: 1205-1253 [Abstract] [Full Text]
Stone, M. R., O'Neill, A., Catino, D., Bloch, R. J. (2005). Specific Interaction of the Actin-binding Domain of Dystrophin with Intermediate Filaments Containing Keratin 19. Mol. Biol. Cell 16: 4280-4293 [Abstract] [Full Text]
Consolino, C. M., Duclos, F., Lee, J., Williamson, R. A., Campbell, K. P., Brooks, S. V. (2005). Muscles of mice deficient in {alpha}-sarcoglycan maintain large masses and near control force values throughout the life span. Physiol. Genomics 22: 244-256 [Abstract] [Full Text]
Muskiewicz, K. R., Frank, N. Y., Flint, A. F., Gussoni, E. (2005). Myogenic Potential of Muscle Side and Main Population Cells after Intravenous Injection into Sub-lethally Irradiated mdx Mice. J. Histochem. Cytochem. 53: 861-873 [Abstract] [Full Text]
Chakkalakal, J. V., Thompson, J., Parks, R. J., Jasmin, B. J. (2005). Molecular, cellular, and pharmacological therapies for Duchenne/Becker muscular dystrophies. FASEB J. 19: 880-891 [Abstract] [Full Text]
Patnaik, S. K., Stanley, P. (2005). Mouse Large Can Modify Complex N- and Mucin O-Glycans on {alpha}-Dystroglycan to Induce Laminin Binding. J. Biol. Chem. 280: 20851-20859 [Abstract] [Full Text]
Kunz, S., Rojek, J. M., Perez, M., Spiropoulou, C. F., Oldstone, M. B. A. (2005). Characterization of the Interaction of Lassa Fever Virus with Its Cellular Receptor {alpha}-Dystroglycan. J. Virol. 79: 5979-5987 [Abstract] [Full Text]
Zhan, Y., Tremblay, M. R., Melian, N., Carbonetto, S. (2005). Evidence That Dystroglycan Is Associated with Dynamin and Regulates Endocytosis. J. Biol. Chem. 280: 18015-18024 [Abstract] [Full Text]
Li, S., Liquari, P., McKee, K. K., Harrison, D., Patel, R., Lee, S., Yurchenco, P. D. (2005). Laminin-sulfatide binding initiates basement membrane assembly and enables receptor signaling in Schwann cells and fibroblasts. JCB 169: 179-189 [Abstract] [Full Text]
Wallquist, W., Plantman, S., Thams, S., Thyboll, J., Kortesmaa, J., Lannergren, J., Domogatskaya, A., Ogren, S. O., Risling, M., Hammarberg, H., Tryggvason, K., Cullheim, S. (2005). Impeded Interaction between Schwann Cells and Axons in the Absence of Laminin {alpha}4. J. Neurosci. 25: 3692-3700 [Abstract] [Full Text]
Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., Hino, H., Suzuki, A., Sanai, Y., Kitajima, K., Sakuraba, H. (2005). Distal Myopathy with Rimmed Vacuoles: Impaired O-Glycan Formation in Muscular Glycoproteins. Am. J. Pathol. 166: 1121-1130 [Abstract] [Full Text]
Scheele, S., Falk, M., Franzen, A., Ellin, F., Ferletta, M., Lonai, P., Andersson, B., Timpl, R., Forsberg, E., Ekblom, P. (2005). Laminin {alpha}1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly. Proc. Natl. Acad. Sci. USA 102: 1502-1506 [Abstract] [Full Text]
Zhou, Y. W., Oak, S. A., Senogles, S. E., Jarrett, H. W. (2005). Laminin-{alpha}1 globular domains 3 and 4 induce heterotrimeric G protein binding to {alpha}-syntrophin's PDZ domain and alter intracellular Ca2+ in muscle. Am. J. Physiol. Cell Physiol. 288: C377-C388 [Abstract] [Full Text]
Esapa, C. T., McIlhinney, R.A. J., Blake, D. J. (2005). Fukutin-related protein mutations that cause congenital muscular dystrophy result in ER-retention of the mutant protein in cultured cells. Hum Mol Genet 14: 295-305 [Abstract] [Full Text]
Hernandez-Gonzalez, E. O., Mornet, D., Rendon, A., Martinez-Rojas, D. (2005). Absence of Dp71 in mdx3cv mouse spermatozoa alters flagellar morphology and the distribution of ion channels and nNOS. J. Cell Sci. 118: 137-145 [Abstract] [Full Text]
Singh, J., Itahana, Y., Knight-Krajewski, S., Kanagawa, M., Campbell, K. P., Bissell, M. J., Muschler, J. (2004). Proteolytic Enzymes and Altered Glycosylation Modulate Dystroglycan Function in Carcinoma Cells. Cancer Res. 64: 6152-6159 [Abstract] [Full Text]
Head, S. I., Bakker, A. J., Liangas, G. (2004). EDL and soleus muscles of the C57BL6J/dy2j laminin-{alpha}2-deficient dystrophic mouse are not vulnerable to eccentric contractions. Exp Physiol 89: 531-539 [Abstract] [Full Text]
Allikian, M. J., Hack, A. A., Mewborn, S., Mayer, U., McNally, E. M. (2004). Genetic compensation for sarcoglycan loss by integrin {alpha}7{beta}1 in muscle. J. Cell Sci. 117: 3821-3830 [Abstract] [Full Text]
Ido, H., Harada, K., Futaki, S., Hayashi, Y., Nishiuchi, R., Natsuka, Y., Li, S., Wada, Y., Combs, A. C., Ervasti, J. M., Sekiguchi, K. (2004). Molecular Dissection of the {alpha}-Dystroglycan- and Integrin-binding Sites within the Globular Domain of Human Laminin-10. J. Biol. Chem. 279: 10946-10954 [Abstract] [Full Text]
Miner, J. H., Li, C., Patton, B. L. (2004). Laminins {alpha}2 and {alpha}4 in Pancreatic Acinar Basement Membranes Are Required for Basal Receptor Localization. J. Histochem. Cytochem. 52: 153-156 [Abstract] [Full Text]
Kanwar, Y. S., Wada, J., Lin, S., Danesh, F. R., Chugh, S. S., Yang, Q., Banerjee, T., Lomasney, J. W. (2004). Update of extracellular matrix, its receptors, and cell adhesion molecules in mammalian nephrogenesis. Am. J. Physiol. Renal Physiol. 286: F202-F215 [Abstract] [Full Text]
Hoyte, K., Jayasinha, V., Xia, B., Martin, P. T. (2004). Transgenic Overexpression of Dystroglycan Does Not Inhibit Muscular Dystrophy in mdx Mice. Am. J. Pathol. 164: 711-718 [Abstract] [Full Text]
Brown, S. C., Torelli, S., Brockington, M., Yuva, Y., Jimenez, C., Feng, L., Anderson, L., Ugo, I., Kroger, S., Bushby, K., Voit, T., Sewry, C., Muntoni, F. (2004). Abnormalities in {alpha}-Dystroglycan Expression in MDC1C and LGMD2I Muscular Dystrophies. Am. J. Pathol. 164: 727-737 [Abstract] [Full Text]
McDearmon, E. L., Combs, A. C., Ervasti, J. M. (2003). Core 1 Glycans on {alpha}-Dystroglycan Mediate Laminin-induced Acetylcholine Receptor Clustering but Not Laminin Binding. J. Biol. Chem. 278: 44868-44873 [Abstract] [Full Text]
Hathaway, H. J., Evans, S. C., Dubois, D. H., Foote, C. I., Elder, B. H., Shur, B. D. (2003). Mutational analysis of the cytoplasmic domain of {beta}1,4-galactosyltransferase I: influence of phosphorylation on cell surface expression. J. Cell Sci. 116: 4319-4330 [Abstract] [Full Text]
Grewal, P. K., Hewitt, J. E. (2003). Glycosylation defects: a new mechanism for muscular dystrophy?. Hum Mol Genet 12: R259-264 [Abstract] [Full Text]
Oak, S. A., Zhou, Y. W., Jarrett, H. W. (2003). Skeletal Muscle Signaling Pathway through the Dystrophin Glycoprotein Complex and Rac1. J. Biol. Chem. 278: 39287-39295 [Abstract] [Full Text]
Moghadaszadeh, B., Albrechtsen, R., Guo, L. T., Zaik, M., Kawaguchi, N., Borup, R. H., Kronqvist, P., Schroder, H. D., Davies, K. E., Voit, T., Nielsen, F. C., Engvall, E., Wewer, U. M. (2003). Compensation for dystrophin-deficiency: ADAM12 overexpression in skeletal muscle results in increased {alpha}7 integrin, utrophin and associated glycoproteins. Hum Mol Genet 12: 2467-2479 [Abstract] [Full Text]
Martin, P. T. (2003). Dystroglycan glycosylation and its role in matrix binding in skeletal muscle. Glycobiology 13: 55R-66R [Abstract] [Full Text]
Durbeej, M., Sawatzki, S. M., Barresi, R., Schmainda, K. M., Allamand, V., Michele, D. E., Campbell, K. P. (2003). Gene transfer establishes primacy of striated vs. smooth muscle sarcoglycan complex in limb-girdle muscular dystrophy. Proc. Natl. Acad. Sci. USA 100: 8910-8915 [Abstract] [Full Text]
Previtali, S. C., Nodari, A., Taveggia, C., Pardini, C., Dina, G., Villa, A., Wrabetz, L., Quattrini, A., Feltri, M. L. (2003). Expression of Laminin Receptors in Schwann Cell Differentiation: Evidence for Distinct Roles. J. Neurosci. 23: 5520-5530 [Abstract] [Full Text]
Takeda, S., Kondo, M., Sasaki, J., Kurahashi, H., Kano, H., Arai, K., Misaki, K., Fukui, T., Kobayashi, K., Tachikawa, M., Imamura, M., Nakamura, Y., Shimizu, T., Murakami, T., Sunada, Y., Fujikado, T., Matsumura, K., Terashima, T., Toda, T. (2003). Fukutin is required for maintenance of muscle integrity, cortical histiogenesis and normal eye development. Hum Mol Genet 12: 1449-1459 [Abstract] [Full Text]
Michele, D. E., Campbell, K. P. (2003). Dystrophin-Glycoprotein Complex: Post-translational Processing and Dystroglycan Function. J. Biol. Chem. 278: 15457-15460 [Full Text]
Guyon, J. R., Mosley, A. N., Zhou, Y., O'Brien, K. F., Sheng, X., Chiang, K., Davidson, A. J., Volinski, J. M., Zon, L. I., Kunkel, L. M. (2003). The dystrophin associated protein complex in zebrafish. Hum Mol Genet 12: 601-615 [Abstract] [Full Text]
Grady, R. M., Akaaboune, M., Cohen, A. L., Maimone, M. M., Lichtman, J. W., Sanes, J. R. (2003). Tyrosine-phosphorylated and nonphosphorylated isoforms of {alpha}-dystrobrevin: roles in skeletal muscle and its neuromuscular and myotendinous junctions. JCB 160: 741-752 [Abstract] [Full Text]
Nawrotzki, R., Willem, M., Miosge, N., Brinkmeier, H., Mayer, U. (2003). Defective integrin switch and matrix composition at alpha 7-deficient myotendinous junctions precede the onset of muscular dystrophy in mice. Hum Mol Genet 12: 483-495 [Abstract] [Full Text]
Le Rumeur, E., Fichou, Y., Pottier, S., Gaboriau, F., Rondeau-Mouro, C., Vincent, M., Gallay, J., Bondon, A. (2003). Interaction of Dystrophin Rod Domain with Membrane Phospholipids. EVIDENCE OF A CLOSE PROXIMITY BETWEEN TRYPTOPHAN RESIDUES AND LIPIDS. J. Biol. Chem. 278: 5993-6001 [Abstract] [Full Text]
Esapa, C. T., Benson, M. A., Schroder, J. E., Martin-Rendon, E., Brockington, M., Brown, S. C., Muntoni, F., Kroger, S., Blake, D. J. (2002). Functional requirements for fukutin-related protein in the Golgi apparatus. Hum Mol Genet 11: 3319-3331 [Abstract] [Full Text]
Muschler, J., Levy, D., Boudreau, R., Henry, M., Campbell, K., Bissell, M. J. (2002). A Role for Dystroglycan in Epithelial Polarization: Loss of Function in Breast Tumor Cells. Cancer Res. 62: 7102-7109 [Abstract] [Full Text]
Austin, R. C., Fox, J. E. B., Werstuck, G. H., Stafford, A. R., Bulman, D. E., Dally, G. Y., Ackerley, C. A., Weitz, J. I., Ray, P. N. (2002). Identification of Dp71 Isoforms in the Platelet Membrane Cytoskeleton. POTENTIAL ROLE IN THROMBIN-MEDIATED PLATELET ADHESION. J. Biol. Chem. 277: 47106-47113 [Abstract] [Full Text]
Roper, K., Gregory, S. L., Brown, N. H. (2002). The `Spectraplakins': cytoskeletal giants with characteristics of both spectrin and plakin families. J. Cell Sci. 115: 4215-4225 [Abstract] [Full Text]
Kikkawa, Y., Moulson, C. L., Virtanen, I., Miner, J. H. (2002). Identification of the Binding Site for the Lutheran Blood Group Glycoprotein on Laminin alpha 5 through Expression of Chimeric Laminin Chains in Vivo. J. Biol. Chem. 277: 44864-44869 [Abstract] [Full Text]
CROSBIE, R. H., BARRESI, R., CAMPBELL, K. P. (2002). Loss of sarcolemma nNOS in sarcoglycan-deficient muscle. FASEB J. 16: 1786-1791 [Abstract] [Full Text]
Holzfeind, P. J., Grewal, P. K., Reitsamer, H. A., Kechvar, J., Lassmann, H., Hoeger, H., Hewitt, J. E., Bittner, R. E. (2002). Skeletal, cardiac and tongue muscle pathology, defective retinal transmission, and neuronal migration defects in the Largemyd mouse defines a natural model for glycosylation-deficient muscle - eye - brain disorders. Hum Mol Genet 11: 2673-2687 [Abstract] [Full Text]
Chamberlain, J. S. (2002). Gene therapy of muscular dystrophy. Hum Mol Genet 11: 2355-2362 [Abstract] [Full Text]
Harper, S. Q., Crawford, R. W., DelloRusso, C., Chamberlain, J. S. (2002). Spectrin-like repeats from dystrophin and {alpha}-actinin-2 are not functionally interchangeable. Hum Mol Genet 11: 1807-1815 [Abstract] [Full Text]
CROSBIE, R. H., DOVICO, S. A., FLANAGAN, J. D., CHAMBERLAIN, J. S., OWNBY, C. L., CAMPBELL, K. P. (2002). Characterization of aquaporin-4 in muscle and muscular dystrophy. FASEB J. 16: 943-949 [Abstract] [Full Text]
Warner, L. E., DelloRusso, C., Crawford, R. W., Rybakova, I. N., Patel, J. R., Ervasti, J. M., Chamberlain, J. S. (2002). Expression of Dp260 in muscle tethers the actin cytoskeleton to the dystrophin-glycoprotein complex and partially prevents dystrophy. Hum Mol Genet 11: 1095-1105 [Abstract] [Full Text]
Rybakova, I. N., Patel, J. R., Davies, K. E., Yurchenco, P. D., Ervasti, J. M. (2002). Utrophin Binds Laterally along Actin Filaments and Can Couple Costameric Actin with Sarcolemma When Overexpressed in Dystrophin-deficient Muscle. Mol. Biol. Cell 13: 1512-1521 [Abstract] [Full Text]