Beta and gamma actin mRNAs are differentially located within myoblasts

doi:10.1083/jcb.122.4.825

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Beta and gamma actin mRNAs are differentially located within myoblasts

MA Hill and P Gunning
Cell Biology Unit, Children's Medical Research Institute, Wentworthville, New South Wales, Australia.

Actin is of fundamental importance to all eukaryotic cells. Of the six mammalian actins, beta (beta) and gamma (gamma) cytoplasmic are the isoforms found in all nonmuscle cells and differ by only four amino acids at the amino-terminal region. Both genes are regulated temporally and spatially, though no differences in protein function have been described. Using fluorescent double in situ hybridization we describe the simultaneous intracellular localization of both beta and gamma actin mRNA. This study shows that myoblasts differentially segregate the beta and gamma actin mRNAs. The distribution of gamma actin mRNA, only to perinuclear and nearby cytoplasm, suggests a distribution based on diffusion or restriction to nearby cytoplasm. The distribution of beta actin mRNA, perinuclear and at the cell periphery, implicates a peripheral localizing signal which is unique to the beta isoform. The peripheral beta actin mRNA corresponded to cellular morphologies, extending processes, and ruffling edges that reflect cell movement. Total actin and gamma actin protein steady-state distributions were identified by specific antibodies. gamma actin protein was found in both stress fibers and at the cell plasma membrane and does not correspond to its mRNA distribution. We suggest that localized protein synthesis rather than steady-state distribution functionally differentiates between the actin isoforms.
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LEE, N. S., SUN, B., WILLIAMSON, R., GUNKEL, N., SALVATERRA, P. M., ROSSI, J. J. (2001). Functional colocalization of ribozymes and target mRNAs in Drosophila oocytes. FASEB J. 15: 2390-2400 [Abstract] [Full Text]
Peckham, M, Miller, G, Wells, C, Zicha, D, Dunn, G. (2001). Specific changes to the mechanism of cell locomotion induced by overexpression of (&bgr;)-actin. J. Cell Sci. 114: 1367-1377 [Abstract]
Gunning, P., Ferguson, V, Brennan, K., Hardeman, E. (2001). Alpha-skeletal actin induces a subset of muscle genes independently of muscle differentiation and withdrawal from the cell cycle. J. Cell Sci. 114: 513-524 [Abstract]
Morris, E., Evason, K, Wiand, C, L'Ecuyer, T., Fulton, A. (2000). Misdirected vimentin messenger RNA alters cell morphology and motility. J. Cell Sci. 113: 2433-2443 [Abstract]
Anderson, M., Muro-Cacho, C., Cordero, J., Livingston, S., Munoz-Antonia, T. (1999). Transforming Growth Factor {beta} Receptors in Verrucous and Squamous Cell Carcinoma. Arch Otolaryngol Head Neck Surg 125: 849-854 [Abstract] [Full Text]
Marion, R. M., Fortes, P., Beloso, A., Dotti, C., Ortin, J. (1999). A Human Sequence Homologue of Staufen Is an RNA-Binding Protein That Is Associated with Polysomes and Localizes to the Rough Endoplasmic Reticulum. Mol. Cell. Biol. 19: 2212-2219 [Abstract] [Full Text]
Nabi, I. (1999). The polarization of the motile cell. J. Cell Sci. 112: 1803-1811 [Abstract]
Kiebler, M. A., Hemraj, I., Verkade, P., Kohrmann, M., Fortes, P., Marion, R. M., Ortin, J., Dotti, C. G. (1999). The Mammalian Staufen Protein Localizes to the Somatodendritic Domain of Cultured Hippocampal Neurons: Implications for Its Involvement in mRNA Transport. J. Neurosci. 19: 288-297 [Abstract] [Full Text]
Potter, D. A., Tirnauer, J. S., Janssen, R., Croall, D. E., Hughes, C. N., Fiacco, K. A., Mier, J. W., Maki, M., Herman, I. M. (1998). Calpain Regulates Actin Remodeling during Cell Spreading. JCB 141: 647-662 [Abstract] [Full Text]
Fages, C, Kaksonen, M, Kinnunen, T, Punnonen, E., Rauvala, H (1998). Regulation of mRNA localization by transmembrane signalling: local interaction of HB-GAM (heparin-binding growth-associated molecule) with the cell surface localizes beta-actin mRNA. J. Cell Sci. 111: 3073-3080 [Abstract]
Watanabe, H, Kislauskis, E., Mackay, C., Mason-Savas, A, Marks, S. (1998). Actin mRNA isoforms are differentially sorted in normal osteoblasts and sorting is altered in osteoblasts from a skeletal mutation in the rat. J. Cell Sci. 111: 1287-1292 [Abstract]
Kaech, S., Fischer, M., Doll, T., Matus, A. (1997). Isoform Specificity in the Relationship of Actin to Dendritic Spines. J. Neurosci. 17: 9565-9572 [Abstract] [Full Text]
Mounier, N, Perriard, J., Gabbiani, G, Chaponnier, C (1997). Transfected muscle and non-muscle actins are differentially sorted by cultured smooth muscle and non-muscle cells. J. Cell Sci. 110: 839-846 [Abstract]
Hofer, D, Ness, W, Drenckhahn, D (1997). Sorting of actin isoforms in chicken auditory hair cells. J. Cell Sci. 110: 765-770 [Abstract]
Marsden, K. M., Doll, T., Ferralli, J., Botteri, F., Matus, A. (1996). Transgenic Expression of Embryonic MAP2 in Adult Mouse Brain: Implications for Neuronal Polarization. J. Neurosci. 16: 3265-3273 [Abstract] [Full Text]
Yao, X., Cheng, L., Forte, J. G. (1996). Biochemical Characterization of Ezrin-Actin Interaction. J. Biol. Chem. 271: 7224-7229 [Abstract] [Full Text]
Veyrune, J., Campbell, G., Wiseman, J, Blanchard, J., Hesketh, J. (1996). A localisation signal in the 3' untranslated region of c-myc mRNA targets c-myc mRNA and beta-globin reporter sequences to the perinuclear cytoplasm and cytoskeletal-bound polysomes. J. Cell Sci. 109: 1185-1194 [Abstract]
Bershadsky, A., Gluck, U, Denisenko, O., Sklyarova, T., Spector, I, Ben-Ze'ev, A (1995). The state of actin assembly regulates actin and vinculin expression by a feedback loop. J. Cell Sci. 108: 1183-1193 [Abstract]
Huttelmaier, S., Illenberger, S., Grosheva, I., Rudiger, M., Singer, R. H., Jockusch, B. M. (2001). Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins. JCB 155: 775-786 [Abstract] [Full Text]