Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts

doi:10.1083/jcb.122.5.1079

This Article

	Full Text (PDF, 3399K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lenormand, P.
	Articles by Pouyssegur, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lenormand, P.
	Articles by Pouyssegur, J.


Social Bookmarking

	What's this?

ARTICLES

Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts

P Lenormand, C Sardet, G Pages, G L'Allemain, A Brunet and J Pouyssegur
Centre de Biochimie-CNRS, Universite de Nice, France.

Mitogen-activated protein kinases (p42mapk and p44mapk) are serine/threonine kinases that are activated rapidly in cells stimulated with various extracellular signals. This activation is mediated via MAP kinase kinase (p45mapkk), a dual specificity kinase which phosphorylates two key regulatory threonine and tyrosine residues of MAP kinases. We reported previously that the persistent phase of MAP kinase activation is essential for mitogenically stimulated cells to pass the "restriction point" of the cell cycle. Here, using specific polyclonal antibodies and transfection of epitope-tagged recombinant MAP kinases we demonstrate that these signaling protein kinases undergo distinct spatio-temporal localization in growth factor-stimulated cells. In G0-arrested hamster fibroblasts the activator p45mapkk and MAP kinases (p42mapk, p44mapk) are mainly cytoplasmic. Subsequent to mitogenic stimulation by serum or alpha-thrombin both MAP kinase isoforms translocate into the nucleus. This translocation is rapid (seen in 15 min), persistent (at least during the entire G1 period up to 6 h), reversible (by removal of the mitogenic stimulus) and apparently 'coupled' to the mitogenic potential; it does not occur in response to nonmitogenic agents such as alpha-thrombin-receptor synthetic peptides and phorbol esters that fail to activate MAP kinases persistently. When p42mapk and p44mapk are expressed stably at high levels, they are found in the nucleus of resting cells; this nuclear localization is also apparent with kinase-deficient mutants (p44mapk T192A or Y194F). In marked contrast the p45mapkk activator remains cytoplasmic even during prolonged growth factor stimulation and even after high expression levels achieved by transfection. We propose that the rapid and persistent nuclear transfer of p42mapk and p44mapk during the entire G0-G1 period is crucial for the function of these kinases in mediating the growth response.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

O'Mahony, F., Alzamora, R., Chung, H.-L., Thomas, W., Harvey, B. J. (2009). Genomic Priming of the Antisecretory Response to Estrogen in Rat Distal Colon throughout the Estrous Cycle. Mol. Endocrinol. 23: 1885-1899 [Abstract] [Full Text]
Caunt, C. J., Armstrong, S. P., Rivers, C. A., Norman, M. R., McArdle, C. A. (2008). Spatiotemporal Regulation of ERK2 by Dual Specificity Phosphatases. J. Biol. Chem. 283: 26612-26623 [Abstract] [Full Text]
Wang, J., Yen, A. (2008). A MAPK-positive Feedback Mechanism for BLR1 Signaling Propels Retinoic Acid-triggered Differentiation and Cell Cycle Arrest. J. Biol. Chem. 283: 4375-4386 [Abstract] [Full Text]
Lefloch, R., Pouyssegur, J., Lenormand, P. (2008). Single and Combined Silencing of ERK1 and ERK2 Reveals Their Positive Contribution to Growth Signaling Depending on Their Expression Levels. Mol. Cell. Biol. 28: 511-527 [Abstract] [Full Text]
Yazicioglu, M. N., Goad, D. L., Ranganathan, A., Whitehurst, A. W., Goldsmith, E. J., Cobb, M. H. (2007). Mutations in ERK2 Binding Sites Affect Nuclear Entry. J. Biol. Chem. 282: 28759-28767 [Abstract] [Full Text]
Lee, N. Y., Blobe, G. C. (2007). The Interaction of Endoglin with beta-Arrestin2 Regulates Transforming Growth Factor-beta-mediated ERK Activation and Migration in Endothelial Cells. J. Biol. Chem. 282: 21507-21517 [Abstract] [Full Text]
Bourguignon, L. Y. W., Gilad, E., Peyrollier, K. (2007). Heregulin-mediated ErbB2-ERK Signaling Activates Hyaluronan Synthases Leading to CD44-dependent Ovarian Tumor Cell Growth and Migration. J. Biol. Chem. 282: 19426-19441 [Abstract] [Full Text]
Burgermeister, E., Chuderland, D., Hanoch, T., Meyer, M., Liscovitch, M., Seger, R. (2007). Interaction with MEK Causes Nuclear Export and Downregulation of Peroxisome Proliferator-Activated Receptor {gamma}. Mol. Cell. Biol. 27: 803-817 [Abstract] [Full Text]
Bogoyevitch, M. A., Kobe, B. (2006). Uses for JNK: the Many and Varied Substrates of the c-Jun N-Terminal Kinases. Microbiol. Mol. Biol. Rev. 70: 1061-1095 [Abstract] [Full Text]
Costa, M., Marchi, M., Cardarelli, F., Roy, A., Beltram, F., Maffei, L., Ratto, G. M. (2006). Dynamic regulation of ERK2 nuclear translocation and mobility in living cells. J. Cell Sci. 119: 4952-4963 [Abstract] [Full Text]
Vukmirica, J., Monzo, P., Le Marchand-Brustel, Y., Cormont, M. (2006). The Rab4A Effector Protein Rabip4 Is Involved in Migration of NIH 3T3 Fibroblasts. J. Biol. Chem. 281: 36360-36368 [Abstract] [Full Text]
Ranganathan, A., Yazicioglu, M. N., Cobb, M. H. (2006). The Nuclear Localization of ERK2 Occurs by Mechanisms Both Independent of and Dependent on Energy. J. Biol. Chem. 281: 15645-15652 [Abstract] [Full Text]
Kondoh, K., Terasawa, K., Morimoto, H., Nishida, E. (2006). Regulation of nuclear translocation of extracellular signal-regulated kinase 5 by active nuclear import and export mechanisms.. Mol. Cell. Biol. 26: 1679-1690 [Abstract] [Full Text]
Caunt, C. J., Finch, A. R., Sedgley, K. R., Oakley, L., Luttrell, L. M., McArdle, C. A. (2006). Arrestin-mediated ERK Activation by Gonadotropin-releasing Hormone Receptors: RECEPTOR-SPECIFIC ACTIVATION MECHANISMS AND COMPARTMENTALIZATION. J. Biol. Chem. 281: 2701-2710 [Abstract] [Full Text]
Marenda, D. R., Vrailas, A. D., Rodrigues, A. B., Cook, S., Powers, M. A., Lorenzen, J. A., Perkins, L. A., Moses, K. (2006). MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing. Development 133: 43-51 [Abstract] [Full Text]
Singh, R. R., Song, C., Yang, Z., Kumar, R. (2005). Nuclear Localization and Chromatin Targets of p21-activated Kinase 1. J. Biol. Chem. 280: 18130-18137 [Abstract] [Full Text]
Refojo, D., Echenique, C., Muller, M. B., Reul, J. M. H. M., Deussing, J. M., Wurst, W., Sillaber, I., Paez-Pereda, M., Holsboer, F., Arzt, E. (2005). Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas. Proc. Natl. Acad. Sci. USA 102: 6183-6188 [Abstract] [Full Text]
Bourguignon, L. Y. W., Gilad, E., Rothman, K., Peyrollier, K. (2005). Hyaluronan-CD44 Interaction with IQGAP1 Promotes Cdc42 and ERK Signaling, Leading to Actin Binding, Elk-1/Estrogen Receptor Transcriptional Activation, and Ovarian Cancer Progression. J. Biol. Chem. 280: 11961-11972 [Abstract] [Full Text]
Mandl, M., Slack, D. N., Keyse, S. M. (2005). Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5. Mol. Cell. Biol. 25: 1830-1845 [Abstract] [Full Text]
Khan, S. M., Oliver, R. H., Yeh, J. (2005). Epidermal Growth Factor Receptor Inhibition by Tyrphostin 51 Induces Apoptosis in Luteinized Granulosa Cells. J. Clin. Endocrinol. Metab. 90: 469-473 [Abstract] [Full Text]
Natalicchio, A., Laviola, L., De Tullio, C., Renna, L. A., Montrone, C., Perrini, S., Valenti, G., Procino, G., Svelto, M., Giorgino, F. (2004). Role of the p66Shc Isoform in Insulin-like Growth Factor I Receptor Signaling through MEK/Erk and Regulation of Actin Cytoskeleton in Rat Myoblasts. J. Biol. Chem. 279: 43900-43909 [Abstract] [Full Text]
Karlsson, M., Mathers, J., Dickinson, R. J., Mandl, M., Keyse, S. M. (2004). Both Nuclear-Cytoplasmic Shuttling of the Dual Specificity Phosphatase MKP-3 and Its Ability to Anchor MAP Kinase in the Cytoplasm Are Mediated by a Conserved Nuclear Export Signal. J. Biol. Chem. 279: 41882-41891 [Abstract] [Full Text]
See, V., Rajala, N. K.M., Spiller, D. G., White, M. R.H. (2004). Calcium-dependent regulation of the cell cycle via a novel MAPK-NF-{kappa}B pathway in Swiss 3T3 cells. JCB 166: 661-672 [Abstract] [Full Text]
Li, S., Gerrard, E. R. Jr., Balkovetz, D. F. (2004). Evidence for ERK1/2 phosphorylation controlling contact inhibition of proliferation in Madin-Darby canine kidney epithelial cells. Am. J. Physiol. Cell Physiol. 287: C432-C439 [Abstract] [Full Text]
Imamura, Y., Katahira, T., Kitamura, D. (2004). Identification and Characterization of a Novel BASH N Terminus-associated Protein, BNAS2. J. Biol. Chem. 279: 26425-26432 [Abstract] [Full Text]
Roux, P. P., Blenis, J. (2004). ERK and p38 MAPK-Activated Protein Kinases: a Family of Protein Kinases with Diverse Biological Functions. Microbiol. Mol. Biol. Rev. 68: 320-344 [Abstract] [Full Text]
Whitehurst, A. W., Robinson, F. L., Moore, M. S., Cobb, M. H. (2004). The Death Effector Domain Protein PEA-15 Prevents Nuclear Entry of ERK2 by Inhibiting Required Interactions. J. Biol. Chem. 279: 12840-12847 [Abstract] [Full Text]
Butcher, G. Q., Lee, B., Obrietan, K. (2003). Temporal Regulation of Light-Induced Extracellular Signal-Regulated Kinase Activation in the Suprachiasmatic Nucleus. J. Neurophysiol. 90: 3854-3863 [Abstract] [Full Text]
Julien, C., Coulombe, P., Meloche, S. (2003). Nuclear Export of ERK3 by a CRM1-dependent Mechanism Regulates Its Inhibitory Action on Cell Cycle Progression. J. Biol. Chem. 278: 42615-42624 [Abstract] [Full Text]
Pei, J.-J., Gong, C.-X., An, W.-L., Winblad, B., Cowburn, R. F., Grundke-Iqbal, I., Iqbal, K. (2003). Okadaic-Acid-Induced Inhibition of Protein Phosphatase 2A Produces Activation of Mitogen-Activated Protein Kinases ERK1/2, MEK1/2, and p70 S6, Similar to That in Alzheimer's Disease. Am. J. Pathol. 163: 845-858 [Abstract] [Full Text]
Kumar, J. P., Hsiung, F., Powers, M. A., Moses, K. (2003). Nuclear translocation of activated MAP kinase is developmentally regulated in the developing Drosophila eye. Development 130: 3703-3714 [Abstract] [Full Text]
Fringer, J., Grinnell, F. (2003). Fibroblast Quiescence in Floating Collagen Matrices: DECREASE IN SERUM ACTIVATION OF MEK AND RAF BUT NOT RAS. J. Biol. Chem. 278: 20612-20617 [Abstract] [Full Text]
Moshkov, I. E., Novikova, G. V., Mur, L. A.J., Smith, A. R., Hall, M. A. (2003). Ethylene Rapidly Up-Regulates the Activities of Both Monomeric GTP-Binding Proteins and Protein Kinase(s) in Epicotyls of Pea. Plant Physiol. 131: 1718-1726 [Abstract] [Full Text]
Beutler, T., Hoflich, C., Stevens, P. A., Kruger, D. H., Prosch, S. (2003). Downregulation of the Epidermal Growth Factor Receptor by Human Cytomegalovirus Infection in Human Fetal Lung Fibroblasts. Am. J. Respir. Cell Mol. Bio. 28: 86-94 [Abstract] [Full Text]
Tu, X., Batta, P., Innocent, N., Prisco, M., Casaburi, I., Belletti, B., Baserga, R. (2002). Nuclear Translocation of Insulin Receptor Substrate-1 by Oncogenes And Igf-I. EFFECT ON RIBOSOMAL RNA SYNTHESIS*. J. Biol. Chem. 277: 44357-44365 [Abstract] [Full Text]
Lai, J.-M., Wu, S., Huang, D.-Y., Chang, Z.-F. (2002). Cytosolic Retention of Phosphorylated Extracellular Signal-Regulated Kinase and a Rho-Associated Kinase-Mediated Signal Impair Expression of p21Cip1/Waf1 in Phorbol 12-Myristate-13- Acetate-Induced Apoptotic Cells. Mol. Cell. Biol. 22: 7581-7592 [Abstract] [Full Text]
Scherl, A., Coute, Y., Deon, C., Calle, A., Kindbeiter, K., Sanchez, J.-C., Greco, A., Hochstrasser, D., Diaz, J.-J. (2002). Functional Proteomic Analysis of Human Nucleolus. Mol. Biol. Cell 13: 4100-4109 [Abstract] [Full Text]
Sulpice, E., Bryckaert, M., Lacour, J., Contreres, J.-O., Tobelem, G. (2002). Platelet factor 4 inhibits FGF2-induced endothelial cell proliferation via the extracellular signal-regulated kinase pathway but not by the phosphatidylinositol 3-kinase pathway. Blood 100: 3087-3094 [Abstract] [Full Text]
Bjorndal, B., Helleland, C., Boe, S.-O., Gudbrandsen, O. A., Kalland, K.-H., Bohov, P., Berge, R. K., Lillehaug, J. R. (2002). Nuclear import of factors involved in signaling is inhibited in C3H/10T1/2 cells treated with tetradecylthioacetic acid. J. Lipid Res. 43: 1630-1640 [Abstract] [Full Text]
Eblen, S. T., Slack, J. K., Weber, M. J., Catling, A. D. (2002). Rac-PAK Signaling Stimulates Extracellular Signal-Regulated Kinase (ERK) Activation by Regulating Formation of MEK1-ERK Complexes. Mol. Cell. Biol. 22: 6023-6033 [Abstract] [Full Text]
Prisco, M., Santini, F., Baffa, R., Liu, M., Drakas, R., Wu, A., Baserga, R. (2002). Nuclear Translocation of Insulin Receptor Substrate-1 by the Simian Virus 40 T Antigen and the Activated Type 1 Insulin-like Growth Factor Receptor. J. Biol. Chem. 277: 32078-32085 [Abstract] [Full Text]
Nguyen, A. N., Ikner, A. D., Shiozaki, M., Warren, S. M., Shiozaki, K. (2002). Cytoplasmic Localization of Wis1 MAPKK by Nuclear Export Signal Is Important for Nuclear Targeting of Spc1/Sty1 MAPK in Fission Yeast. Mol. Biol. Cell 13: 2651-2663 [Abstract] [Full Text]
Oksvold, M. P., Skarpen, E., Widerberg, J., Huitfeldt, H. S. (2002). Fluorescent Histochemical Techniques for Analysis of Intracellular Signaling. J. Histochem. Cytochem. 50: 289-303 [Abstract] [Full Text]
Robinson, M. J., Xu, B.-e, Stippec, S., Cobb, M. H. (2002). Different Domains of the Mitogen-activated Protein Kinases ERK3 and ERK2 Direct Subcellular Localization and Upstream Specificity in Vivo. J. Biol. Chem. 277: 5094-5100 [Abstract] [Full Text]
Brennan, J. A., Volle, D. J., Chaika, O. V., Lewis, R. E. (2002). Phosphorylation Regulates the Nucleocytoplasmic Distribution of Kinase Suppressor of Ras. J. Biol. Chem. 277: 5369-5377 [Abstract] [Full Text]
Stanciu, M., DeFranco, D. B. (2002). Prolonged Nuclear Retention of Activated Extracellular Signal-regulated Protein Kinase Promotes Cell Death Generated by Oxidative Toxicity or Proteasome Inhibition in a Neuronal Cell Line. J. Biol. Chem. 277: 4010-4017 [Abstract] [Full Text]
Young, M. R., Nair, R., Bucheimer, N., Tulsian, P., Brown, N., Chapp, C., Hsu, T.-C., Colburn, N. H. (2002). Transactivation of Fra-1 and Consequent Activation of AP-1 Occur Extracellular Signal-Regulated Kinase Dependently. Mol. Cell. Biol. 22: 587-598 [Abstract] [Full Text]
Aplin, A. E., Hogan, B. P., Tomeu, J., Juliano, R. L. (2002). Cell adhesion differentially regulates the nucleocytoplasmic distribution of active MAP kinases. J. Cell Sci. 115: 2781-2790 [Abstract] [Full Text]
Esparis-Ogando, A., Diaz-Rodriguez, E., Montero, J. C., Yuste, L., Crespo, P., Pandiella, A. (2002). Erk5 Participates in Neuregulin Signal Transduction and Is Constitutively Active in Breast Cancer Cells Overexpressing ErbB2. Mol. Cell. Biol. 22: 270-285 [Abstract] [Full Text]
Lu, Q., Dunn, R. L., Angeles, R., Smith, G. D. (2002). Regulation of Spindle Formation by Active Mitogen-Activated Protein Kinase and Protein Phosphatase 2A During Mouse Oocyte Meiosis. Biol. Reprod. 66: 29-37 [Abstract] [Full Text]
Yang, Z., Madinova, A., Kozai, T., Joch, H., Aebi, U., Luscher, T. F (2002). Felodipine inhibits nuclear translocation of p42/44 mitogen-activated protein kinase and human smooth muscle cell growth. Cardiovasc Res 53: 227-231 [Abstract] [Full Text]
Cha, H., Lee, E. K., Shapiro, P. (2001). Identification of a C-terminal Region That Regulates Mitogen-activated Protein Kinase Kinase-1 Cytoplasmic Localization and ERK Activation. J. Biol. Chem. 276: 48494-48501 [Abstract] [Full Text]
Matsubayashi, Y., Fukuda, M., Nishida, E. (2001). Evidence for Existence of a Nuclear Pore Complex-mediated, Cytosol-independent Pathway of Nuclear Translocation of ERK MAP Kinase in Permeabilized Cells. J. Biol. Chem. 276: 41755-41760 [Abstract] [Full Text]
Carlson, C. J., Fan, Z., Gordon, S. E., Booth, F. W. (2001). Time course of the MAPK and PI3-kinase response within 24 h of skeletal muscle overload. J. Appl. Physiol. 91: 2079-2087 [Abstract] [Full Text]
Aggeli, I.-K. S., Gaitanaki, C., Lazou, A., Beis, I. (2001). Stimulation of multiple MAPK pathways by mechanical overload in the perfused amphibian heart. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281: R1689-R1698 [Abstract] [Full Text]
Masuda, K., Shima, H., Watanabe, M., Kikuchi, K. (2001). MKP-7, a Novel Mitogen-activated Protein Kinase Phosphatase, Functions as a Shuttle Protein. J. Biol. Chem. 276: 39002-39011 [Abstract] [Full Text]
Christopher, J., Velarde, V., Jaffa, A. A. (2001). Induction of B1-Kinin Receptors in Vascular Smooth Muscle Cells: Cellular Mechanisms of MAP Kinase Activation. Hypertension 38: 602-605 [Abstract] [Full Text]
Xu, A., Suh, P.-G., Marmy-Conus, N., Pearson, R. B., Seok, O. Y., Cocco, L., Gilmour, R. S. (2001). Phosphorylation of Nuclear Phospholipase C {beta}1 by Extracellular Signal-Regulated Kinase Mediates the Mitogenic Action of Insulin-Like Growth Factor I. Mol. Cell. Biol. 21: 2981-2990 [Abstract] [Full Text]
Aplin, A. E., Stewart, S. A., Assoian, R. K., Juliano, R.L. (2001). Integrin-Mediated Adhesion Regulates ERK Nuclear Translocation and Phosphorylation of Elk-1. JCB 153: 273-282 [Abstract] [Full Text]
Pearson, G., Robinson, F., Beers Gibson, T., Xu, B.-e, Karandikar, M., Berman, K., Cobb, M. H. (2001). Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions. Endocr. Rev. 22: 153-183 [Abstract] [Full Text]
Furuno, T., Hirashima, N., Onizawa, S., Sagiya, N., Nakanishi, M. (2001). Nuclear Shuttling of Mitogen-Activated Protein (MAP) Kinase (Extracellular Signal-Regulated Kinase (ERK) 2) Was Dynamically Controlled by MAP/ERK Kinase After Antigen Stimulation in RBL-2H3 Cells. J. Immunol. 166: 4416-4421 [Abstract] [Full Text]
Morgan, M. A., Dolp, O., Reuter, C. W. M. (2001). Cell-cycle-dependent activation of mitogen-activated protein kinase kinase (MEK-1/2) in myeloid leukemia cell lines and induction of growth inhibition and apoptosis by inhibitors of RAS signaling. Blood 97: 1823-1834 [Abstract] [Full Text]
Yamamoto, K., Dang, Q. N., Maeda, Y., Huang, H., Kelly, R. A., Lee, R. T. (2001). Regulation of Cardiomyocyte Mechanotransduction by the Cardiac Cycle. Circulation 103: 1459-1464 [Abstract] [Full Text]
Webb, D. J., Thomas, K. S., Gonias, S. L. (2001). Plasminogen Activator Inhibitor 1 Functions as a Urokinase Response Modifier at the Level of Cell Signaling and Thereby Promotes Mcf-7 Cell Growth. JCB 152: 741-752 [Abstract] [Full Text]
Volmat, V., Camps, M., Arkinstall, S., Pouyssegur, J., Lenormand, P. (2001). The nucleus, a site for signal termination by sequestration and inactivation of p42/p44 MAP kinases. J. Cell Sci. 114: 3433-3443 [Abstract] [Full Text]
Lorenzen, J., Baker, S., Denhez, F, Melnick, M., Brower, D., Perkins, L. (2001). Nuclear import of activated D-ERK by DIM-7, an importin family member encoded by the gene moleskin. Development 128: 1403-1414 [Abstract]
Eblen, S. T., Catling, A. D., Assanah, M. C., Weber, M. J. (2001). Biochemical and Biological Functions of the N-Terminal, Noncatalytic Domain of Extracellular Signal-Regulated Kinase 2. Mol. Cell. Biol. 21: 249-259 [Abstract] [Full Text]
Zhou, X., Richon, V. M., Wang, A. H., Yang, X.-J., Rifkind, R. A., Marks, P. A. (2000). Histone deacetylase 4 associates with extracellular signal-regulated kinases 1 and 2, and its cellular localization is regulated by oncogenic Ras. Proc. Natl. Acad. Sci. USA 10.1073/pnas.250494697v1 [Abstract] [Full Text]
Ruwhof, C., van der Laarse, A. (2000). Mechanical stress-induced cardiac hypertrophy: mechanisms and signal transduction pathways. Cardiovasc Res 47: 23-37 [Abstract] [Full Text]
Davis, S., Vanhoutte, P., Pages, C., Caboche, J., Laroche, S. (2000). The MAPK/ERK Cascade Targets Both Elk-1 and cAMP Response Element-Binding Protein to Control Long-Term Potentiation-Dependent Gene Expression in the Dentate Gyrus In Vivo. J. Neurosci. 20: 4563-4572 [Abstract] [Full Text]
Rovida, E., Marra, F., Baccarini, M., Sbarba, P. D. (2000). Constitutive activation of the MAPK pathway mediates v-fes-induced mitogenesis in murine macrophages. Blood 95: 3959-3963 [Abstract] [Full Text]
Mattison, C. P., Ota, I. M. (2000). Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Genes Dev. 14: 1229-1235 [Abstract] [Full Text]
Hu, X., Moscinski, L. C., Valkov, N. I., Fisher, A. B., Hill, B. J., Zuckerman, K. S. (2000). Prolonged Activation of the Mitogen-activated Protein Kinase Pathway Is Required for Macrophage-like Differentiation of a Human Myeloid Leukemic Cell Line. Cell Growth Differ. 11: 191-200 [Abstract] [Full Text]
Dews, M., Prisco, M., Peruzzi, F., Romano, G., Morrione, A., Baserga, R. (2000). Domains of the Insulin-Like Growth Factor I Receptor Required for the Activation of Extracellular Signal-Regulated Kinases. Endocrinology 141: 1289-1300 [Abstract] [Full Text]
Morrione, A., Romano, G., Navarro, M., Reiss, K., Valentinis, B., Dews, M., Eves, E., Rosner, M. R., Baserga, R. (2000). Insulin-like Growth Factor I Receptor Signaling in Differentiation of Neuronal H19-7 Cells. Cancer Res. 60: 2263-2272 [Abstract] [Full Text]
Huang, Y., Li, H., Gupta, R., Morris, P. C., Luan, S., Kieber, J. J. (2000). ATMPK4, an Arabidopsis Homolog of Mitogen-Activated Protein Kinase, Is Activated in Vitro by AtMEK1 through Threonine Phosphorylation. Plant Physiol. 122: 1301-1310 [Abstract] [Full Text]
Adachi, M., Fukuda, M., Nishida, E. (2000). Nuclear Export of Map Kinase (ERK) Involves a Map Kinase Kinase (Mek-Dependent) Active Transport Mechanism. JCB 148: 849-856 [Abstract] [Full Text]
Bogoyevitch, M. A. (2000). Signalling via stress-activated mitogen-activated protein kinases in the cardiovascular system. Cardiovasc Res 45: 826-842 [Abstract] [Full Text]
Guo, N, Faller, D., Denis, G. (2000). Activation-induced nuclear translocation of RING3. J. Cell Sci. 113: 3085-3091 [Abstract]
Janody, F, Sturny, R, Catala, F, Desplan, C, Dostatni, N (2000). Phosphorylation of bicoid on MAP-kinase sites: contribution to its interaction with the torso pathway. Development 127: 279-289 [Abstract]
Shirakata, Y., Ishii, K., Yagita, H., Okumura, K., Taniguchi, M., Takemori, T. (1999). Distinct Subcellular Localization and Substrate Specificity of Extracellular Signal-Regulated Kinase in B Cells upon Stimulation with IgM and CD40. J. Immunol. 163: 6589-6597 [Abstract] [Full Text]
Kranenburg, O., Verlaan, I., Moolenaar, W. H. (1999). Dynamin Is Required for the Activation of Mitogen-activated Protein (MAP) Kinase by MAP Kinase Kinase. J. Biol. Chem. 274: 35301-35304 [Abstract] [Full Text]
Hochholdinger, F., Baier, G., Nogalo, A., Bauer, B., Grunicke, H. H., Uberall, F. (1999). Novel Membrane-Targeted ERK1 and ERK2 Chimeras Which Act as Dominant Negative, Isotype-Specific Mitogen-Activated Protein Kinase Inhibitors of Ras-Raf-Mediated Transcriptional Activation of c-fos in NIH 3T3 Cells. Mol. Cell. Biol. 19: 8052-8065 [Abstract] [Full Text]
Mattison, C. P., Spencer, S. S., Kresge, K. A., Lee, J., Ota, I. M. (1999). Differential Regulation of the Cell Wall Integrity Mitogen-Activated Protein Kinase Pathway in Budding Yeast by the Protein Tyrosine Phosphatases Ptp2 and Ptp3. Mol. Cell. Biol. 19: 7651-7660 [Abstract] [Full Text]
Wu, W., Graves, L. M., Jaspers, I., Devlin, R. B., Reed, W., Samet, J. M. (1999). Activation of the EGF receptor signaling pathway in human airway epithelial cells exposed to metals. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L924-L931 [Abstract] [Full Text]
Rubinfeld, H., Hanoch, T., Seger, R. (1999). Identification of a Cytoplasmic-Retention Sequence in ERK2. J. Biol. Chem. 274: 30349-30352 [Abstract] [Full Text]
Zheng, C., Xiang, J., Hunter, T., Lin, A. (1999). The JNKK2-JNK1 Fusion Protein Acts As a Constitutively Active c-Jun Kinase That Stimulates c-Jun Transcription Activity. J. Biol. Chem. 274: 28966-28971 [Abstract] [Full Text]
Peruzzi, F., Prisco, M., Dews, M., Salomoni, P., Grassilli, E., Romano, G., Calabretta, B., Baserga, R. (1999). Multiple Signaling Pathways of the Insulin-Like Growth Factor 1 Receptor in Protection from Apoptosis. Mol. Cell. Biol. 19: 7203-7215 [Abstract] [Full Text]
Aliaga, J. C., Deschenes, C., Beaulieu, J.-F., Calvo, E. L., Rivard, N. (1999). Requirement of the MAP kinase cascade for cell cycle progression and differentiation of human intestinal cells. Am. J. Physiol. Gastrointest. Liver Physiol. 277: G631-G641 [Abstract] [Full Text]
Oh, P., Schnitzer, J. E. (1999). Immunoisolation of Caveolae with High Affinity Antibody Binding to the Oligomeric Caveolin Cage. TOWARD UNDERSTANDING THE BASIS OF PURIFICATION. J. Biol. Chem. 274: 23144-23154 [Abstract] [Full Text]
Zuniga, A., Torres, J., Ubeda, J., Pulido, R. (1999). Interaction of Mitogen-activated Protein Kinases with the Kinase Interaction Motif of the Tyrosine Phosphatase PTP-SL Provides Substrate Specificity and Retains ERK2 in the Cytoplasm. J. Biol. Chem. 274: 21900-21907 [Abstract] [Full Text]
Chiloeches, A., Paterson, H. F., Marais, R., Clerk, A., Marshall, C. J., Sugden, P. H. (1999). Regulation of Ras{middle dot}GTP Loading and Ras-Raf Association in Neonatal Rat Ventricular Myocytes by G Protein-coupled Receptor Agonists and Phorbol Ester. ACTIVATION OF THE EXTRACELLULAR SIGNAL-REGULATED KINASE CASCADE BY PHORBOL ESTER IS MEDIATED BY Ras. J. Biol. Chem. 274: 19762-19770 [Abstract] [Full Text]
Fernandes, D., Guida, E., Koutsoubos, V., Harris, T., Vadiveloo, P., Wilson, J. W., Stewart, A. G. (1999). Glucocorticoids Inhibit Proliferation, Cyclin D1 Expression, and Retinoblastoma Protein Phosphorylation, but Not Activity of the Extracellular-Regulated Kinases in Human Cultured Airway Smooth Muscle. Am. J. Respir. Cell Mol. Bio. 21: 77-88 [Abstract] [Full Text]
Xing, C., Imagawa, W. (1999). Altered MAP kinase (ERK1,2) regulation in primary cultures of mammary tumor cells: elevated basal activity and sustained response to EGF. Carcinogenesis 20: 1201-1208 [Abstract] [Full Text]
Ping, P., Zhang, J., Cao, X., Li, R. C.�X., Kong, D., Tang, X.-L., Qiu, Y., Manchikalapudi, S., Auchampach, J. A., Black, R. G., Bolli, R. (1999). PKC-dependent activation of p44/p42 MAPKs during myocardial ischemia-reperfusion in conscious rabbits. Am. J. Physiol. Heart Circ. Physiol. 276: H1468-H1481 [Abstract] [Full Text]
Choi, K.-Y., Kranz, J. E., Mahanty, S. K., Park, K.-S., Elion, E. A. (1999). Characterization of Fus3 Localization: Active Fus3 Localizes in Complexes of Varying Size and Specific Activity. Mol. Biol. Cell 10: 1553-1568 [Abstract] [Full Text]
Gotoh, I., Fukuda, M., Adachi, M., Nishida, E. (1999). Control of the Cell Morphology and the S Phase Entry by Mitogen-activated Protein Kinase Kinase. A REGULATORY ROLE OF ITS N-TERMINAL REGION. J. Biol. Chem. 274: 11874-11880 [Abstract] [Full Text]
Improta-Brears, T., Whorton, A. R., Codazzi, F., York, J. D., Meyer, T., McDonnell, D. P. (1999). Estrogen-induced activation of mitogen-activated protein kinase requires mobilization of intracellular calcium. Proc. Natl. Acad. Sci. USA 96: 4686-4691 [Abstract] [Full Text]
Schaeffer, H. J., Weber, M. J. (1999). Mitogen-Activated Protein Kinases: Specific Messages from Ubiquitous Messengers. Mol. Cell. Biol. 19: 2435-2444 [Full Text]