Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor

doi:10.1083/jcb.122.5.993

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Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor

JJ Blow
ICRF Clare Hall Laboratories, Potters Bar, Herts, England.

Xenopus egg extracts treated with the protein kinase inhibitor 6- dimethylaminopurine (6-DMAP) are unable to support the initiation of DNA replication. Nuclei assembled in 6-DMAP extracts behave as though they are in G2, and will not undergo another round of DNA replication until passage through mitosis. 6-DMAP extracts are functionally devoid of a replication factor that modifies chromatin in early G1 before nuclear envelope assembly, but which is itself incapable of crossing the nuclear envelope. This chromatin modification is capable of supporting only a single round of semiconservative replication. The behavior of this replication factor is sufficient to explain why eukaryotic DNA is replicated once and only once in each cell cycle, and conforms to the previous model of a Replication Licensing Factor. Cell cycle analysis shows that this putative Licensing Factor is inactive during metaphase, but becomes rapidly activated on exit from metaphase when it can modify chromatin before nuclear envelope assembly is complete.
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Takahashi, T. S., Basu, A., Bermudez, V., Hurwitz, J., Walter, J. C. (2008). Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts. Genes Dev. 22: 1894-1905 [Abstract] [Full Text]
Lin, D. I., Aggarwal, P., Diehl, J. A. (2008). Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex. Proc. Natl. Acad. Sci. USA 105: 8079-8084 [Abstract] [Full Text]
Takahashi, T. S., Walter, J. C. (2005). Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication. Genes Dev. 19: 2295-2300 [Abstract] [Full Text]
Tsuyama, T., Tada, S., Watanabe, S., Seki, M., Enomoto, T. (2005). Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts. Nucleic Acids Res 33: 765-775 [Abstract] [Full Text]
Arias, E. E., Walter, J. C. (2005). Replication-dependent destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts. Genes Dev. 19: 114-126 [Abstract] [Full Text]
Detivaud, L., Pascreau, G., Karaiskou, A., Osborne, H. B., Kubiak, J. Z. (2003). Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation in Xenopus laevis egg extracts. J. Cell Sci. 116: 2697-2705 [Abstract] [Full Text]
Blow, J. J., Gillespie, P. J., Francis, D., Jackson, D. A. (2001). Replication Origins in XenopusEgg Extract Are 5-15 Kilobases Apart and Are Activated in Clusters That Fire at Different Times. JCB 152: 15-26 [Abstract] [Full Text]
Jares, P., Blow, J. J. (2000). Xenopus Cdc7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading. Genes Dev. 14: 1528-1540 [Abstract] [Full Text]
Kuwahara, K., Yoshida, M., Kondo, E., Sakata, A., Watanabe, Y., Abe, E., Kouno, Y., Tomiyasu, S., Fujimura, S., Tokuhisa, T., Kimura, H., Ezaki, T., Sakaguchi, N. (2000). A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication. Blood 95: 2321-2328 [Abstract] [Full Text]
Maiorano, D., Lemaitre, J.-M., Mechali, M. (2000). Stepwise Regulated Chromatin Assembly of MCM2-7 Proteins. J. Biol. Chem. 275: 8426-8431 [Abstract] [Full Text]
Winkler, K. E., Swenson, K. I., Kornbluth, S., Means, A. R. (2000). Requirement of the Prolyl Isomerase Pin1 for the Replication Checkpoint. Science 287: 1644-1647 [Abstract] [Full Text]
Romanowski, P., Marr, J., Madine, M. A., Rowles, A., Blow, J. J., Gautier, J., Laskey, R. A. (2000). Interaction of Xenopus Cdc2{middle dot}Cyclin A1 with the Origin Recognition Complex. J. Biol. Chem. 275: 4239-4243 [Abstract] [Full Text]
Prokhorova, T. A., Blow, J. J. (2000). Sequential MCM/P1 Subcomplex Assembly Is Required to Form a Heterohexamer with Replication Licensing Activity. J. Biol. Chem. 275: 2491-2498 [Abstract] [Full Text]
Liu, Q., Choe, W.-c., Campbell, J. L. (2000). Identification of the Xenopus laevis Homolog of Saccharomyces cerevisiae DNA2 and Its Role in DNA Replication. J. Biol. Chem. 275: 1615-1624 [Abstract] [Full Text]
Gillespie, P. J., Blow, J. J. (2000). Nucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication. Nucleic Acids Res 28: 472-480 [Abstract] [Full Text]
Li, C., Bogan, J., Natale, D., DePamphilis, M. (2000). Selective activation of pre-replication complexes in vitro at specific sites in mammalian nuclei. J. Cell Sci. 113: 887-898 [Abstract]
Sun, W, Hola, M, Pedley, K, Tada, S, Blow, J., Todorov, I., Kearsey, S., Brooks, R. (2000). The replication capacity of intact mammalian nuclei in Xenopus egg extracts declines with quiescence, but the residual DNA synthesis is independent of Xenopus MCM proteins. J. Cell Sci. 113: 683-695 [Abstract]
De Souza, C. P. C., Ye, X. S., Osmani, S. A. (1999). Checkpoint Defects Leading to Premature Mitosis Also Cause Endoreplication of DNA in Aspergillus nidulans. Mol. Biol. Cell 10: 3661-3674 [Abstract] [Full Text]
Neuber, E., Havari, E., Sanchez, J. A., Powers, R. D., Wangh, L. J. (1999). Efficient Human Sperm Pronucleus Formation and Replication in Xenopus Egg Extracts. Biol. Reprod. 61: 912-920 [Abstract] [Full Text]
Rowles, A, Tada, S, Blow, J. (1999). Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins. J. Cell Sci. 112: 2011-2018 [Abstract]
Tugal, T., Zou-Yang, X. H., Gavin, K., Pappin, D., Canas, B., Kobayashi, R., Hunt, T., Stillman, B. (1998). The Orc4p and Orc5p Subunits of the Xenopus and Human Origin Recognition Complex Are Related to Orc1p and Cdc6p. J. Biol. Chem. 273: 32421-32429 [Abstract] [Full Text]
Michael, W. M., Newport, J. (1998). Coupling of Mitosis to the Completion of S Phase Through Cdc34-Mediated Degradation of Wee1. Science 282: 1886-1889 [Abstract] [Full Text]
Aladjem, M. I., Rodewald, L. W., Kolman, J. L., Wahl, G. M. (1998). Genetic Dissection of a Mammalian Replicator in the Human $beta$ -Globin Locus. Science 281: 1005-1009 [Abstract] [Full Text]
Lu, Z. H., Sittman, D. B., Romanowski, P., Leno, G. H. (1998). Histone H1 Reduces the Frequency of Initiation in Xenopus Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin. Mol. Biol. Cell 9: 1163-1176 [Abstract] [Full Text]
Yew, P. R., Kirschner, M. W. (1997). Proteolysis and DNA Replication: The CDC34 Requirement in the Xenopus Egg Cell Cycle. Science 277: 1672-1676 [Abstract] [Full Text]
Pasero, P, Braguglia, D, Gasser, S M (1997). ORC-dependent and origin-specific initiation of DNA replication at defined foci in isolated yeast nuclei.. Genes Dev. 11: 1504-1518 [Abstract]
Donovan, S., Harwood, J., Drury, L. S., Diffley, J. F.�X. (1997). Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in�budding�yeast. Proc. Natl. Acad. Sci. USA 94: 5611-5616 [Abstract] [Full Text]
Hua, X. H., Yan, H., Newport, J. (1997). A Role for Cdk2 Kinase in Negatively Regulating DNA Replication during S Phase of the Cell Cycle. JCB 137: 183-192 [Abstract] [Full Text]
Mahbubani, H. M., Chong, J. P.J., Chevalier, S., Thommes, P., Blow, J. J. (1997). Cell Cycle Regulation of the Replication Licensing System: Involvement of a Cdk-dependent Inhibitor. JCB 136: 125-135 [Abstract] [Full Text]
Strausfeld, U., Howell, M, Descombes, P, Chevalier, S, Rempel, R., Adamczewski, J, Maller, J., Hunt, T, Blow, J. (1996). Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts. J. Cell Sci. 109: 1555-1563 [Abstract]
Jones, C, Smythe, C (1996). Activation of the Xenopus cyclin degradation machinery by full-length cyclin A. J. Cell Sci. 109: 1071-1079 [Abstract]
Okishio, N, Adachi, Y, Yanagida, M (1996). Fission yeast Nda1 and Nda4, MCM homologs required for DNA replication, are constitutive nuclear proteins. J. Cell Sci. 109: 319-326 [Abstract]
Sanchez, J. A., Wonsey, D. R., Harris, L., Morales, J., Wangh, L. J. (1995). Efficient Plasmid DNA Replication in Xenopus Egg Extracts Does Not Depend on Prior Chromatin Assembly. J. Biol. Chem. 270: 29676-29681 [Abstract] [Full Text]
Sauer, K, Knoblich, J A, Richardson, H, Lehner, C F (1995). Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis.. Genes Dev. 9: 1327-1339 [Abstract]
Chevalier, S, Tassan, J., Cox, R, Philippe, M, Ford, C (1995). Both cdc2 and cdk2 promote S phase initiation in Xenopus egg extracts. J. Cell Sci. 108: 1831-1841 [Abstract]
Starborg, M, Brundell, E, Gell, K, Larsson, C, White, I, Daneholt, B, Hoog, C (1995). A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication. J. Cell Sci. 108: 927-934 [Abstract]
Murphy, J, Crompton, C., Hainey, S, Codd, G., Hutchison, C. (1995). The role of protein phosphorylation in the assembly of a replication competent nucleus: investigations in Xenopus egg extracts using the cyanobacterial toxin microcystin-LR. J. Cell Sci. 108: 235-244 [Abstract]
Hutchison, C., Bridger, J., Cox, L., Kill, I. (1994). Weaving a pattern from disparate threads: lamin function in nuclear assembly and DNA replication. J. Cell Sci. 107: 3259-3269 [Abstract]
Kelly, T.J., Nurse, P., Forsburg, S.L. (1993). Coupling DNA Replication to the Cell Cycle. Cold Spring Harb Symp Quant Biol 58: 637-644 [Abstract]
Chuang, L.-C., Yew, P. R. (2001). Regulation of Nuclear Transport and Degradation of the Xenopus Cyclin-dependent Kinase Inhibitor, p27Xic1. J. Biol. Chem. 276: 1610-1617 [Abstract] [Full Text]