Protein transport to the dendritic plasma membrane of cultured neurons is regulated by rab8p

doi:10.1083/jcb.123.1.47

This Article

	Full Text (PDF, 3028K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Huber, L. A.
	Articles by Dotti, C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Huber, L. A.
	Articles by Dotti, C.


Social Bookmarking

	What's this?

ARTICLES

Protein transport to the dendritic plasma membrane of cultured neurons is regulated by rab8p

LA Huber, MJ de Hoop, P Dupree, M Zerial, K Simons and C Dotti
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

In the companion paper (Huber, L. A., S. W. Pimplikar, R. G. Parton, H. Virta, M. Zerial, and K. Simons. J. Cell Biol. 123:35-45) we reported that the small GTPase rab8p is involved in transport from the TGN to the basolateral plasma membrane in epithelia. In the present work we investigated the localization and function of rab8p in polarized hippocampal neurons. By immunofluorescence microscopy we found that rab8p localized preferentially in the somatodendritic domain, and was excluded from the axon. Double-labeling immunofluorescence showed that some of the rab8p co-localized in the dendrites with the Semliki Forest Virus glycoprotein E2 (SFV-E2). An antisense oligonucleotide approach was used to investigate the role of rab8p in dendritic transport of newly synthesized viral glycoproteins. Antisense oligonucleotides corresponding to the initiation region of the rab8 coding sequence were added to the cultured neurons for four days. This treatment resulted in a significant decrease in cellular levels of rab8p and transport of SFV- E2 from the cell body to the dendrites was significantly reduced. However, no effect was observed on axonal transport of influenza HA. From these results we conclude that rab8p is involved in transport of proteins to the dendritic surface in neurons.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Linder, M. D., Mayranpaa, M. I., Peranen, J., Pietila, T. E., Pietiainen, V. M., Uronen, R.-L., Olkkonen, V. M., Kovanen, P. T., Ikonen, E. (2009). Rab8 Regulates ABCA1 Cell Surface Expression and Facilitates Cholesterol Efflux in Primary Human Macrophages. Arterioscler. Thromb. Vasc. Bio. 29: 883-888 [Abstract] [Full Text]
Nielsen, E., Cheung, A. Y., Ueda, T. (2008). The Regulatory RAB and ARF GTPases for Vesicular Trafficking. Plant Physiol. 147: 1516-1526 [Full Text]
Henry, L., Sheff, D. R. (2008). Rab8 Regulates Basolateral Secretory, But Not Recycling, Traffic at the Recycling Endosome. Mol. Biol. Cell 19: 2059-2068 [Abstract] [Full Text]
Yamamura, R., Nishimura, N., Nakatsuji, H., Arase, S., Sasaki, T. (2008). The Interaction of JRAB/MICAL-L2 with Rab8 and Rab13 Coordinates the Assembly of Tight Junctions and Adherens Junctions. Mol. Biol. Cell 19: 971-983 [Abstract] [Full Text]
Swiatecka-Urban, A., Talebian, L., Kanno, E., Moreau-Marquis, S., Coutermarsh, B., Hansen, K., Karlson, K. H., Barnaby, R., Cheney, R. E., Langford, G. M., Fukuda, M., Stanton, B. A. (2007). Myosin Vb Is Required for Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator in Rab11a-specific Apical Recycling Endosomes in Polarized Human Airway Epithelial Cells. J. Biol. Chem. 282: 23725-23736 [Abstract] [Full Text]
Jacobs, T., Causeret, F., Nishimura, Y. V., Terao, M., Norman, A., Hoshino, M., Nikolic, M. (2007). Localized Activation of p21-Activated Kinase Controls Neuronal Polarity and Morphology. J. Neurosci. 27: 8604-8615 [Abstract] [Full Text]
Linder, M. D., Uronen, R.-L., Holtta-Vuori, M., van der Sluijs, P., Peranen, J., Ikonen, E. (2007). Rab8-dependent Recycling Promotes Endosomal Cholesterol Removal in Normal and Sphingolipidosis Cells. Mol. Biol. Cell 18: 47-56 [Abstract] [Full Text]
Sahlender, D. A., Roberts, R. C., Arden, S. D., Spudich, G., Taylor, M. J., Luzio, J. P., Kendrick-Jones, J., Buss, F. (2005). Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis. JCB 169: 285-295 [Abstract] [Full Text]
Mitsui, S., Saito, M., Hayashi, K., Mori, K., Yoshihara, Y. (2005). A Novel Phenylalanine-Based Targeting Signal Directs Telencephalin to Neuronal Dendrites. J. Neurosci. 25: 1122-1131 [Abstract] [Full Text]
Santoro, B., Wainger, B. J., Siegelbaum, S. A. (2004). Regulation of HCN Channel Surface Expression by a Novel C-Terminal Protein-Protein Interaction. J. Neurosci. 24: 10750-10762 [Abstract] [Full Text]
Hwang, H.-H., Gelvin, S. B. (2004). Plant Proteins That Interact with VirB2, the Agrobacterium tumefaciens Pilin Protein, Mediate Plant Transformation. Plant Cell 16: 3148-3167 [Abstract] [Full Text]
Gerges, N. Z., Backos, D. S., Esteban, J. A. (2004). Local Control of AMPA Receptor Trafficking at the Postsynaptic Terminal by a Small GTPase of the Rab Family. J. Biol. Chem. 279: 43870-43878 [Abstract] [Full Text]
Vernoud, V., Horton, A. C., Yang, Z., Nielsen, E. (2003). Analysis of the Small GTPase Gene Superfamily of Arabidopsis. Plant Physiol. 131: 1191-1208 [Abstract] [Full Text]
Rodriguez, O. C., Cheney, R. E. (2002). Human myosin-Vc is a novel class V myosin expressed in epithelial cells. J. Cell Sci. 115: 991-1004 [Abstract] [Full Text]
Moritz, O. L., Tam, B. M., Hurd, L. L., Peranen, J., Deretic, D., Papermaster, D. S. (2001). Mutant rab8 Impairs Docking and Fusion of Rhodopsin-bearing Post-Golgi Membranes and Causes Cell Death of Transgenic Xenopus Rods. Mol. Biol. Cell 12: 2341-2351 [Abstract] [Full Text]
SIMONS, M., SAFFRICH, R., REISER, J., MUNDEL, P. (1999). Directed Membrane Transport Is Involved in Process Formation in Cultured Podocytes. J. Am. Soc. Nephrol. 10: 1633-1639 [Abstract] [Full Text]
Kobayashi, N., Reiser, J., Kriz, W., Kuriyama, R., Mundel, P. (1998). Nonuniform Microtubular Polarity Established by CHO1/MKLP1 Motor Protein Is Necessary for Process Formation of Podocytes. JCB 143: 1961-1970 [Abstract] [Full Text]
Su, T, Stanley, K. (1998). Opposite sorting and transcytosis of the polymeric immunoglobulin receptor in transfected endothelial and epithelial cells. J. Cell Sci. 111: 1197-1206 [Abstract]
Salas, P. J.I., Rodriguez, M. L., Viciana, A. L., Vega-Salas, D. E., Hauri, H.-P. (1997). The Apical Submembrane Cytoskeleton Participates in the Organization of the Apical Pole in Epithelial Cells. JCB 137: 359-375 [Abstract] [Full Text]
Jin, M., Saucan, L., Farquhar, M. G., Palade, G. E. (1996). Rab1a and Multiple Other Rab Proteins Are Associated with the Transcytotic Pathway in Rat Liver. J. Biol. Chem. 271: 30105-30113 [Abstract] [Full Text]
Wilson, A. L., Erdman, R. A., Maltese, W. A. (1996). Association of Rab1B with GDP-dissociation Inhibitor (GDI) Is Required for Recycling but Not Initial Membrane Targeting of the Rab Protein. J. Biol. Chem. 271: 10932-10940 [Abstract] [Full Text]
McConlogue, L., Castellano, F., deWit, C., Schenk, D., Maltese, W. A. (1996). Differential Effects of a Rab6 Mutant on Secretory Versus Amyloidogenic Processing of Alzheimer's beta-Amyloid Precursor Protein. J. Biol. Chem. 271: 1343-1348 [Abstract] [Full Text]
Armstrong, J, Thompson, N, Squire, J., Smith, J, Hayes, B, Solari, R (1996). Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3. J. Cell Sci. 109: 1265-1274 [Abstract]
De Strooper, B., Craessaerts, K., Dewachter, I., Moechars, D., Greenberg, B., Van Leuven, F., Van Den Berghe, H. (1995). Basolateral Secretion of Amyloid Precursor Protein in Madin-Darby Canine Kidney Cells Is Disturbed by Alterations of Intracellular pH and by Introducing a Mutation Associated with Familial Alzheimer's Disease. J. Biol. Chem. 270: 4058-4065 [Abstract] [Full Text]
Simon, J.-P., Ivanov, I.E., Ren, M., Zeng, J., Shopsin, B., Hersh, D., Tempst, P., Erdjument-Bromage, H., Lui, M., De Lemos-Chiarandini, C., Rosenfeld, M., Gravotta, D., Morimoto, T., Adesnik, M., Sabatini, D.D. (1995). Regulation of Post-Golgi Vesicle Production in an In Vitro System. Cold Spring Harb Symp Quant Biol 60: 179-195 [Abstract]
Deretic, D, Huber, L., Ransom, N, Mancini, M, Simons, K, Papermaster, D. (1995). rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis. J. Cell Sci. 108: 215-224 [Abstract]
Lutcke, A, Parton, R., Murphy, C, Olkkonen, V., Dupree, P, Valencia, A, Simons, K, Zerial, M (1994). Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression. J. Cell Sci. 107: 3437-3448 [Abstract]