MIF2 is required for mitotic spindle integrity during anaphase spindle elongation in Saccharomyces cerevisiae

doi:10.1083/jcb.123.2.387

This Article

Full Text (PDF, 2882K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Brown, M. T.

Articles by Hartwell, L. H.

Search for Related Content

PubMed

PubMed Citation

Articles by Brown, M. T.

Articles by Hartwell, L. H.

Pubmed/NCBI databases

Hazardous Substances DB
Gene	GEO Profiles
HomoloGene	Protein
Compound via MeSH
Substance via MeSH
CARBENDAZIM

Social Bookmarking

What's this?

ARTICLES

MIF2 is required for mitotic spindle integrity during anaphase spindle elongation in Saccharomyces cerevisiae

MT Brown, L Goetsch and LH Hartwell
Department of Genetics, University of Washington, Seattle 98195.

The function of the essential MIF2 gene in the Saccharomyces cerevisiae cell cycle was examined by overepressing or creating a deficit of MIF2 gene product. When MIF2 was overexpressed, chromosomes missegregated during mitosis and cells accumulated in the G2 and M phases of the cell cycle. Temperature sensitive mutants isolated by in vitro mutagenesis delayed cell cycle progression when grown at the restrictive temperature, accumulated as large budded cells that had completed DNA replication but not chromosome segregation, and lost viability as they passed through mitosis. Mutant cells also showed increased levels of mitotic chromosome loss, supersensitivity to the microtubule destabilizing drug MBC, and morphologically aberrant spindles. mif2 mutant spindles arrested development immediately before anaphase spindle elongation, and then frequently broke apart into two disconnected short half spindles with misoriented spindle pole bodies. These findings indicate that MIF2 is required for structural integrity of the spindle during anaphase spindle elongation. The deduced Mif2 protein sequence shared no extensive homologies with previously identified proteins but did contain a short region of homology to a motif involved in binding AT rich DNA by the Drosophila D1 and mammalian HMGI chromosomal proteins.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Milks, K. J., Moree, B., Straight, A. F. (2009). Dissection of CENP-C-directed Centromere and Kinetochore Assembly. Mol. Biol. Cell 20: 4246-4255 [Abstract] [Full Text]
Cohen, R. L., Espelin, C. W., De Wulf, P., Sorger, P. K., Harrison, S. C., Simons, K. T. (2008). Structural and Functional Dissection of Mif2p, a Conserved DNA-binding Kinetochore Protein. Mol. Biol. Cell 19: 4480-4491 [Abstract] [Full Text]
Schwartz, D. C., Felberbaum, R., Hochstrasser, M. (2007). The Ulp2 SUMO Protease Is Required for Cell Division following Termination of the DNA Damage Checkpoint. Mol. Cell. Biol. 27: 6948-6961 [Abstract] [Full Text]
Kwon, M.-S., Hori, T., Okada, M., Fukagawa, T. (2007). CENP-C Is Involved in Chromosome Segregation, Mitotic Checkpoint Function, and Kinetochore Assembly. Mol. Biol. Cell 18: 2155-2168 [Abstract] [Full Text]
Liao, W.-T., Song, L.-B., Zhang, H.-Z., Zhang, X., Zhang, L., Liu, W.-L., Feng, Y., Guo, B.-H., Mai, H.-Q., Cao, S.-M., Li, M.-Z., Qin, H.-D., Zeng, Y.-X., Zeng, M.-S. (2007). Centromere Protein H Is a Novel Prognostic Marker for Nasopharyngeal Carcinoma Progression and Overall Patient Survival. Clin. Cancer Res. 13: 508-514 [Abstract] [Full Text]
Kanta, H., Laprade, L., Almutairi, A., Pinto, I. (2006). Suppressor Analysis of a Histone Defect Identifies a New Function for the Hda1 Complex in Chromosome Segregation. Genetics 173: 435-450 [Abstract] [Full Text]
Heeger, S., Leismann, O., Schittenhelm, R., Schraidt, O., Heidmann, S., Lehner, C. F. (2005). Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog. Genes Dev. 19: 2041-2053 [Abstract] [Full Text]
Tomonaga, T., Matsushita, K., Ishibashi, M., Nezu, M., Shimada, H., Ochiai, T., Yoda, K., Nomura, F. (2005). Centromere Protein H Is Up-regulated in Primary Human Colorectal Cancer and Its Overexpression Induces Aneuploidy. Cancer Res. 65: 4683-4689 [Abstract] [Full Text]
Stoyan, T., Carbon, J. (2004). Inner Kinetochore of the Pathogenic Yeast Candida glabrata. Eukaryot Cell 3: 1154-1163 [Abstract] [Full Text]
Espelin, C. W., Simons, K. T., Harrison, S. C., Sorger, P. K. (2003). Binding of the Essential Saccharomyces cerevisiae Kinetochore Protein Ndc10p to CDEII. Mol. Biol. Cell 14: 4557-4568 [Abstract] [Full Text]
Hsu, J.-m., Huang, J., Meluh, P. B., Laurent, B. C. (2003). The Yeast RSC Chromatin-Remodeling Complex Is Required for Kinetochore Function in Chromosome Segregation. Mol. Cell. Biol. 23: 3202-3215 [Abstract] [Full Text]
McCleland, M. L., Gardner, R. D., Kallio, M. J., Daum, J. R., Gorbsky, G. J., Burke, D. J., Stukenberg, P. T. (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 17: 101-114 [Abstract] [Full Text]
Taylor, D. L., Ho, J. C. Y., Oliver, A., Watts, F. Z. (2002). Cell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific protease. J. Cell Sci. 115: 1113-1122 [Abstract] [Full Text]
Biggins, S., Bhalla, N., Chang, A., Smith, D. L., Murray, A. W. (2001). Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae. Genetics 159: 453-470 [Abstract] [Full Text]
Fukagawa, T., Regnier, V., Ikemura, T. (2001). Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells. Nucleic Acids Res 29: 3796-3803 [Abstract] [Full Text]
Gardner, R. D., Poddar, A., Yellman, C., Tavormina, P. A., Monteagudo, M. C., Burke, D. J. (2001). The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain. Genetics 157: 1493-1502 [Abstract] [Full Text]
Hemmerich, P., Stoyan, T., Wieland, G., Koch, M., Lechner, J., Diekmann, S. (2000). Interaction of yeast kinetochore proteins with centromere-protein/transcription factor Cbf1. Proc. Natl. Acad. Sci. USA 97: 12583-12588 [Abstract] [Full Text]
Sugata, N., Li, S., Earnshaw, W. C., Yen, T. J., Yoda, K., Masumoto, H., Munekata, E., Warburton, P. E., Todokoro, K. (2000). Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes. Hum Mol Genet 9: 2919-2926 [Abstract] [Full Text]
Howman, E. V., Fowler, K. J., Newson, A. J., Redward, S., MacDonald, A. C., Kalitsis, P., Choo, K. H. A. (2000). Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. Proc. Natl. Acad. Sci. USA 97: 1148-1153 [Abstract] [Full Text]
Dawe, R. K., Reed, L. M., Yu, H.-G., Muszynski, M. G., Hiatt, E. N. (1999). A Maize Homolog of Mammalian CENPC Is a Constitutive Component of the Inner Kinetochore. Plant Cell 11: 1227-1238 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Ortiz, J., Stemmann, O., Rank, S., Lechner, J. (1999). A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 13: 1140-1155 [Abstract] [Full Text]
Han, S. J., Lee, Y. C., Gim, B. S., Ryu, G.-H., Park, S. J., Lane, W. S., Kim, Y.-J. (1999). Activator-Specific Requirement of Yeast Mediator Proteins for RNA Polymerase II Transcriptional Activation. Mol. Cell. Biol. 19: 979-988 [Abstract] [Full Text]
Rao-Naik, C., delaCruz, W., Laplaza, J. M., Tan, S., Callis, J., Fisher, A. J. (1998). The Rub Family of Ubiquitin-like Proteins. CRYSTAL STRUCTURE OF ARABIDOPSIS RUB1 AND EXPRESSION OF MULTIPLE RUBS IN ARABIDOPSIS. J. Biol. Chem. 273: 34976-34982 [Abstract] [Full Text]
Kamitani, T., Kito, K., Nguyen, H. P., Fukuda-Kamitani, T., Yeh, E. T.�H. (1998). Characterization of a Second Member of the Sentrin Family of Ubiquitin-like Proteins. J. Biol. Chem. 273: 11349-11353 [Abstract] [Full Text]
Hudson, D. F., Fowler, K. J., Earle, E., Saffery, R., Kalitsis, P., Trowell, H., Hill, J., Wreford, N. G., de Kretser, D. M., Cancilla, M. R., Howman, E., Hii, L., Cutts, S. M., Irvine, D. V., Choo, K.H.A. (1998). Centromere Protein B Null Mice are Mitotically and Meiotically Normal but Have Lower Body and Testis Weights. JCB 141: 309-319 [Abstract] [Full Text]
Kalitsis, P., Fowler, K. J., Earle, E., Hill, J., Choo, K. H.�A. (1998). Targeted disruption of mouse centromere protein C gene leads to mitotic disarray and early embryo�death. Proc. Natl. Acad. Sci. USA 95: 1136-1141 [Abstract] [Full Text]
Meluh, P. B., Koshland, D. (1997). Budding yeast centromere composition and assembly as revealed by in vivo�cross-linking. Genes Dev. 11: 3401-3412 [Abstract] [Full Text]
Kamitani, T., Nguyen, H. P., Yeh, E. T.�H. (1997). Preferential Modification of Nuclear Proteins by a Novel Ubiquitin-like Molecule. J. Biol. Chem. 272: 14001-14004 [Abstract] [Full Text]
Megonigal, M. D., Fertala, J., Bjornsti, M.-A. (1997). Alterations in the Catalytic Activity of Yeast DNA Topoisomerase I Result in Cell Cycle Arrest and Cell Death. J. Biol. Chem. 272: 12801-12808 [Abstract] [Full Text]
Bauer, A, Kolling, R (1996). The SAC3 gene encodes a nuclear protein required for normal progression of mitosis. J. Cell Sci. 109: 1575-1583 [Abstract]
Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G., Earnshaw, W. C. (1995). The Centromere: Hub of Chromosomal Activities. Science 270: 1591-1594 [Abstract]
Nissen, M. S., Reeves, R. (1995). Changes in Superhelicity Are Introduced into Closed Circular DNA by Binding of High Mobility Group Protein I/Y. J. Biol. Chem. 270: 4355-4360 [Abstract] [Full Text]
Ouspenski, I. I., Mueller, U. W., Matynia, A., Sazer, S., Elledge, S. J., Brinkley, B. R. (1995). Ran-binding Protein-1 Is an Essential Component of the Ran/RCC1 Molecular Switch System in Budding Yeast. J. Biol. Chem. 270: 1975-1978 [Abstract] [Full Text]
Dai, K.-S., Liew, C.-C. (2001). A Novel Human Striated Muscle RING Zinc Finger Protein, SMRZ, Interacts with SMT3b via Its RING Domain. J. Biol. Chem. 276: 23992-23999 [Abstract] [Full Text]