Induction of a secondary body axis in Xenopus by antibodies to beta- catenin

doi:10.1083/jcb.123.2.477

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Induction of a secondary body axis in Xenopus by antibodies to beta- catenin

PD McCrea, WM Brieher and BM Gumbiner
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.

We have obtained evidence that a known intracellular component of the cadherin cell-cell adhesion machinery, beta-catenin, contributes to the development of the body axis in the frog Xenopus laevis. Vertebrate beta-catenin is homologous to the Drosophila segment polarity gene product armadillo, and to vertebrate plakoglobin (McCrea, P. D., C. W. Turck, and B. Gumbiner. 1991. Science (Wash. DC). 254: 1359-1361.). Beta-Catenin was found present in all Xenopus embryonic stages examined, and associated with C-cadherin, the major cadherin present in early Xenopus embryos. To test beta-catenin's function, affinity purified Fab fragments were injected into ventral blastomeres of developing four-cell Xenopus embryos. A dramatic phenotype, the duplication of the dorsoanterior embryonic axis, was observed. Furthermore, Fab injections were capable of rescuing dorsal features in UV-ventralized embryos. Similar phenotypes have been observed in misexpression studies of the Wnt and other gene products, suggesting that beta-catenin participates in a signaling pathway which specifies embryonic patterning.
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Montross, W., Ji, H, McCrea, P. (2000). A beta-catenin/engrailed chimera selectively suppresses Wnt signaling. J. Cell Sci. 113: 1759-1770 [Abstract]
Charpentier, E., Lavker, R. M., Acquista, E., Cowin, P. (2000). Plakoglobin Suppresses Epithelial Proliferation and Hair Growth in Vivo. JCB 149: 503-520 [Abstract] [Full Text]
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Dominguez, I, Green, J. (2000). Dorsal downregulation of GSK3beta by a non-Wnt-like mechanism is an early molecular consequence of cortical rotation in early Xenopus embryos. Development 127: 861-868 [Abstract]
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Efstathiou, J. A., Pignatelli, M. (1998). Modulation of Epithelial Cell Adhesion in Gastrointestinal Homeostasis. Am. J. Pathol. 153: 341-347 [Full Text]
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Ratcliffe, M. J., Rubin, L. L., Staddon, J. M. (1997). Dephosphorylation of the Cadherin-associated p100/p120 Proteins in Response to Activation of Protein Kinase C in Epithelial Cells. J. Biol. Chem. 272: 31894-31901 [Abstract] [Full Text]
Salomon, D., Sacco, P. A., Roy, S. G., Simcha, I., Johnson, K. R., Wheelock, M. J., Ben-Ze'ev, A. (1997). Regulation of {beta}-Catenin Levels and Localization by Overexpression of Plakoglobin and Inhibition of the Ubiquitin-Proteasome System. JCB 139: 1325-1335 [Abstract] [Full Text]
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JAWHARI, A., FARTHING, M., PIGNATELLI, M. (1997). The importance of the E-cadherin-catenin complex in the maintenance of intestinal epithelial homoeostasis: more than intercellular glue?. Gut 41: 581-584 [Full Text]
Ilyas, M., Tomlinson, I. P.�M., Rowan, A., Pignatelli, M., Bodmer, W. F. (1997). beta -Catenin mutations in cell lines established from human colorectal�cancers. Proc. Natl. Acad. Sci. USA 94: 10330-10334 [Abstract] [Full Text]
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Torres, M., Stoykova, A., Huber, O., Chowdhury, K., Bonaldo, P., Mansouri, A., Butz, S., Kemler, R., Gruss, P. (1997). An alpha -E-catenin gene trap mutation defines its function in preimplantation�development. Proc. Natl. Acad. Sci. USA 94: 901-906 [Abstract] [Full Text]
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Yost, C, Torres, M, Miller, J R, Huang, E, Kimelman, D, Moon, R T (1996). The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.. Genes Dev. 10: 1443-1454 [Abstract]
Witcher, L. L., Collins, R., Puttagunta, S., Mechanic, S. E., Munson, M., Gumbiner, B., Cowin, P. (1996). Desmosomal Cadherin Binding Domains of Plakoglobin. J. Biol. Chem. 271: 10904-10909 [Abstract] [Full Text]
Hermiston, M L, Wong, M H, Gordon, J I (1996). Forced expression of E-cadherin in the mouse intestinal epithelium slows cell migration and provides evidence for nonautonomous regulation of cell fate in a self-renewing system.. Genes Dev. 10: 985-996 [Abstract]
Hatzfeld, M, Nachtsheim, C (1996). Cloning and characterization of a new armadillo family member, p0071, associated with the junctional plaque: evidence for a subfamily of closely related proteins. J. Cell Sci. 109: 2767-2778 [Abstract]
Holowacz, T, Elinson, R. (1995). Properties of the dorsal activity found in the vegetal cortical cytoplasm of Xenopus eggs. Development 121: 2789-2798 [Abstract]
Cui, Y, Brown, J., Moon, R., Christian, J. (1995). Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis. Development 121: 2177-2186 [Abstract]
Sokol, S., Klingensmith, J, Perrimon, N, Itoh, K (1995). Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled. Development 121: 1637-1647 [Abstract]
Fagotto, F, Gumbiner, B. (1994). Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries. Development 120: 3667-3679 [Abstract]
Hatzfeld, M, Kristjansson, G., Plessmann, U, Weber, K (1994). Band 6 protein, a major constituent of desmosomes from stratified epithelia, is a novel member of the armadillo multigene family. J. Cell Sci. 107: 2259-2270 [Abstract]
Shimamura, K, Hirano, S, McMahon, A., Takeichi, M (1994). Wnt-1-dependent regulation of local E-cadherin and alpha N-catenin expression in the embryonic mouse brain. Development 120: 2225-2234 [Abstract]
Peifer, M, Sweeton, D, Casey, M, Wieschaus, E (1994). wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo. Development 120: 369-380 [Abstract]
Peifer, M (1993). Cancer, catenins, and cuticle pattern: a complex connection. Science 262: 1667-1668
Niessen, C. M., Gumbiner, B. M. (2002). Cadherin-mediated cell sorting not determined by binding or adhesion specificity. JCB 156: 389-400 [Abstract] [Full Text]