Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal

doi:10.1083/jcb.123.3.523

This Article

	Full Text (PDF, 3758K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Whalen, A. M.
	Articles by Steward, R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Whalen, A. M.
	Articles by Steward, R.


Social Bookmarking

	What's this?

ARTICLES

Dissociation of the dorsal-cactus complex and phosphorylation of the dorsal protein correlate with the nuclear localization of dorsal

AM Whalen and R Steward
Department of Molecular Biology, Princeton University, New Jersey 08544.

The formation of dorsal-ventral polarity in Drosophila requires the asymmetric nuclear localization of the dorsal protein along the D/V axis. This process is regulated by the action of the dorsal group genes and cactus. We show that dorsal and cactus are both phosphoproteins that form a stable cytoplasmic complex, and that the cactus protein is stabilized by its interaction with dorsal. The dorsal-cactus complex dissociates when dorsal is targeted to the nucleus. While the phosphorylation of cactus remains apparently unchanged during early embryogenesis, the phosphorylation state of dorsal correlates with its release from cactus and with its nuclear localization. This differential phosphorylation event is regulated by the dorsal group pathway.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Ching, W., Hang, H. C., Nusse, R. (2008). Lipid-independent Secretion of a Drosophila Wnt Protein. J. Biol. Chem. 283: 17092-17098 [Abstract] [Full Text]
Sakaguchi, A., Karachentsev, D., Seth-Pasricha, M., Druzhinina, M., Steward, R. (2008). Functional Characterization of the Drosophila Hmt4-20/Suv4-20 Histone Methyltransferase. Genetics 179: 317-322 [Abstract] [Full Text]
Mindorff, E. N., O'Keefe, D. D., Labbe, A., Yang, J. P., Ou, Y., Yoshikawa, S., van Meyel, D. J. (2007). A Gain-of-Function Screen for Genes That Influence Axon Guidance Identifies the NF-{kappa}B Protein Dorsal and Reveals a Requirement for the Kinase Pelle in Drosophila Photoreceptor Axon Targeting. Genetics 176: 2247-2263 [Abstract] [Full Text]
Koizumi, K., Higashida, H., Yoo, S., Islam, M. S., Ivanov, A. I., Guo, V., Pozzi, P., Yu, S.-H., Rovescalli, A. C., Tang, D., Nirenberg, M. (2007). RNA interference screen to identify genes required for Drosophila embryonic nervous system development. Proc. Natl. Acad. Sci. USA 104: 5626-5631 [Abstract] [Full Text]
Matova, N., Anderson, K. V. (2006). Rel/NF-{kappa}B double mutants reveal that cellular immunity is central to Drosophila host defense. Proc. Natl. Acad. Sci. USA 103: 16424-16429 [Abstract] [Full Text]
Minakhina, S., Steward, R. (2006). Melanotic Mutants in Drosophila: Pathways and Phenotypes. Genetics 174: 253-263 [Abstract] [Full Text]
Karachentsev, D., Sarma, K., Reinberg, D., Steward, R. (2005). PR-Set7-dependent methylation of histone H4 Lys 20 functions in repression of gene expression and is essential for mitosis. Genes Dev. 19: 431-435 [Abstract] [Full Text]
Hou, S., Guan, H., Ricciardi, R. P. (2003). Phosphorylation of Serine 337 of NF-{kappa}B p50 Is Critical for DNA Binding. J. Biol. Chem. 278: 45994-45998 [Abstract] [Full Text]
Maier, H. J., Marienfeld, R., Wirth, T., Baumann, B. (2003). Critical Role of RelB Serine 368 for Dimerization and p100 Stabilization. J. Biol. Chem. 278: 39242-39250 [Abstract] [Full Text]
Rice, J. C., Nishioka, K., Sarma, K., Steward, R., Reinberg, D., Allis, C. D. (2002). Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes. Genes Dev. 16: 2225-2230 [Abstract] [Full Text]
Fernandez, N. Q., Grosshans, J., Goltz, J. S., Stein, D. (2001). Separable and redundant regulatory determinants in Cactus mediate its dorsal group dependent degradation. Development 128: 2963-2974 [Abstract] [Full Text]
Kim, Y.-S., Han, S.-J., Ryu, J.-H., Choi, K.-H., Hong, Y.-S., Chung, Y.-H., Perrot, S., Raibaud, A., Brey, P. T., Lee, W.-J. (2000). Lipopolysaccharide-activated Kinase, an Essential Component for the Induction of the Antimicrobial Peptide Genes in Drosophila melanogaster Cells. J. Biol. Chem. 275: 2071-2079 [Abstract] [Full Text]
Araujo, H, Bier, E (2000). sog and dpp exert opposing maternal functions to modify toll signaling and pattern the dorsoventral axis of the Drosophila embryo. Development 127: 3631-3644 [Abstract]
Drier, E. A., Huang, L. H., Steward, R. (1999). Nuclear import of the Drosophila Rel protein Dorsal is regulated by phosphorylation. Genes Dev. 13: 556-568 [Abstract] [Full Text]
Briggs, L. J., Stein, D., Goltz, J., Corrigan, V. C., Efthymiadis, A., Hubner, S., Jans, D. A. (1998). The cAMP-dependent Protein Kinase Site (Ser312) Enhances Dorsal Nuclear Import through Facilitating Nuclear Localization Sequence/Importin Interaction. J. Biol. Chem. 273: 22745-22752 [Abstract] [Full Text]
Nicolas, E., Reichhart, J. M., Hoffmann, J. A., Lemaitre, B. (1998). In Vivo Regulation of the Ikappa B Homologue cactus during the Immune Response of Drosophila. J. Biol. Chem. 273: 10463-10469 [Abstract] [Full Text]
Stein, D, Goltz, J., Jurcsak, J, Stevens, L (1998). The Dorsal-related immunity factor (Dif) can define the dorsal-ventral axis of polarity in the Drosophila embryo. Development 125: 2159-2169 [Abstract]
Qiu, P, Pan, P., Govind, S (1998). A role for the Drosophila Toll/Cactus pathway in larval hematopoiesis. Development 125: 1909-1920 [Abstract]
Yang, J., Steward, R. (1997). A multimeric complex and the nuclear targeting of the Drosophila Rel protein�Dorsal. Proc. Natl. Acad. Sci. USA 94: 14524-14529 [Abstract] [Full Text]
Liu, Z.-P., Galindo, R. L., Wasserman, S. A. (1997). A role for CKII phosphorylation of the Cactus PEST domain in dorsoventral patterning of the Drosophila�embryo. Genes Dev. 11: 3413-3422 [Abstract] [Full Text]
Edwards, D., Towb, P, Wasserman, S. (1997). An activity-dependent network of interactions links the Rel protein Dorsal with its cytoplasmic regulators. Development 124: 3855-3864 [Abstract]
Norris, J L, Manley, J L (1996). Functional interactions between the pelle kinase, Toll receptor, and tube suggest a mechanism for activation of dorsal.. Genes Dev. 10: 862-872 [Abstract]
Belvin, M P, Jin, Y, Anderson, K V (1995). Cactus protein degradation mediates Drosophila dorsal-ventral signaling.. Genes Dev. 9: 783-793 [Abstract]
Norris, J L, Manley, J L (1995). Regulation of dorsal in cultured cells by Toll and tube: tube function involves a novel mechanism.. Genes Dev. 9: 358-369 [Abstract]
Galindo, R., Edwards, D., Gillespie, S., Wasserman, S. (1995). Interaction of the pelle kinase with the membrane-associated protein tube is required for transduction of the dorsoventral signal in Drosophila embryos. Development 121: 2209-2218 [Abstract]
Carrasco, D, Weih, F, Bravo, R (1994). Developmental expression of the mouse c-rel proto-oncogene in hematopoietic organs. Development 120: 2991-3004 [Abstract]