Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris

doi:10.1083/jcb.123.3.535

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Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris

AP Spong and S Subramani
Department of Biology, University of California, San Diego, La Jolla 92093-0322.

The biogenesis and maintenance of cellular organelles is of fundamental importance in all eukaryotic cells. One such organelle is the peroxisome. The establishment of a genetic system to study peroxisome biogenesis in the methylotrophic yeast Pichia pastoris has yielded many different complementation groups of peroxisomal assembly (pas) or peroxisome-deficient (per) mutants. Each appears to be deficient in functional peroxisomes. One of these mutants, pas5, has been characterized, complemented, and the gene sequenced. Ultrastructural studies show that normal peroxisomes are not present in pas5, but aberrant peroxisomal structures resembling "membranous ghosts" are frequently observed. The "peroxisome ghosts" appear to be induced and segregated to daughter cells normally. Biochemical fractionation analysis of organelles of the pas5 mutant reveals that peroxisomal matrix enzymes are induced normally but are found mostly in the cytosol. However, purification of peroxisome ghosts from the mutant shows that small amounts (< 5%) of matrix enzymes are imported. The PAS5 gene was cloned and found to encode a 127-kD protein, which contains a 200-amino acid-long region of homology with PAS1, NEM- sensitive factor (NSF), and other related ATPases. Weak homology to a yeast myosin was also observed. The gene is not essential for growth on glucose but is essential for growth on oleic acid and methanol. The role of PAS5 in peroxisome biogenesis is discussed.
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Sakai, Y., Koller, A., Rangell, L. K., Keller, G. A., Subramani, S. (1998). Peroxisome Degradation by Microautophagy in Pichia pastoris: Identification of Specific Steps and Morphological Intermediates. JCB 141: 625-636 [Abstract] [Full Text]
Faber, K. N., Heyman, J. A., Subramani, S. (1998). Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with Different Subcellular Membranous Structures Distinct from Peroxisomes. Mol. Cell. Biol. 18: 936-943 [Abstract] [Full Text]
SUBRAMANI, S. (1998). Components Involved in Peroxisome Import, Biogenesis, Proliferation, Turnover, and Movement. Physiol. Rev. 78: 171-188 [Abstract] [Full Text]
Waterham, H. R., Russell, K. A., Vries, Y. d., Cregg, J. M. (1997). Peroxisomal Targeting, Import, and Assembly of Alcohol Oxidase in Pichia pastoris. JCB 139: 1419-1431 [Abstract] [Full Text]
Wiemer, ErikA.C., Luers, GeorgH., Faber, K., Wenzel, T., Veenhuis, M., Subramani, S. (1996). Isolation and Characterization of Pas2p, a Peroxisomal Membrane Protein Essential for Peroxisome Biogenesis in the Methylotrophic Yeast Pichia pastoris. J. Biol. Chem. 271: 18973-18980 [Abstract] [Full Text]
Eitzen, G. A., Aitchison, J. D., Szilard, R. K., Veenhuis, M., Nuttley, W. M., Rachubinski, R. A. (1995). The Yarrowia lipolytica Gene PAY2 Encodes a 42-kDa Peroxisomal Integral Membrane Protein Essential for Matrix Protein Import and Peroxisome Enlargement but Not for Peroxisome Membrane Proliferation. J. Biol. Chem. 270: 1429-1436 [Abstract] [Full Text]
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Hill, P., Walton, P. (1995). Import of microinjected proteins bearing the SKL peroxisomal targeting sequence into the peroxisomes of a human fibroblast cell line: evidence that virtually all peroxisomes are import-competent. J. Cell Sci. 108: 1469-1476 [Abstract]
Tuttle, D., Dunn, W. (1995). Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris. J. Cell Sci. 108: 25-35 [Abstract]