Tenascin is a cytoadhesive extracellular matrix component of the human hematopoietic microenvironment

doi:10.1083/jcb.123.4.1027

This Article

	Full Text (PDF, 2963K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Klein, G.
	Articles by Muller, C. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Klein, G.
	Articles by Muller, C. A.


Social Bookmarking

	What's this?

ARTICLES

Tenascin is a cytoadhesive extracellular matrix component of the human hematopoietic microenvironment

G Klein, S Beck and CA Muller
University Medical Clinic, Department of Internal Medicine II, Tubingen, Federal Republic of Germany.

Tenascin is a large extracellular matrix (ECM) glycoprotein found in restricted tissue locations in the adult organism. It is copiously synthesized in regenerative organs or regenerating tissues and by certain tumors. We have analyzed the expression of tenascin in human long term bone marrow cultures as well as in cryostat sections of native bone marrow and found it strongly expressed by the stromal cells of the microenvironment. Two different protein subunits of 280 and 220 kD were detected by immunoblotting. These two forms are derived most likely from two different mRNA splice variants of 6 and 8 kb detected by Northern blotting. The in vivo analysis of cryostat sections showed a codistribution with other ECM molecules such as fibronectin and collagen type III in the microenvironment surrounding the maturing hematopoietic cells. Using two independent cell adhesion assays tenascin could be shown to function as a cytoadhesive molecule for hematopoietic cells. These data suggest a direct involvement of tenascin in the retention of hematopoietic progenitor cells in the stroma.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Tanaka, R., Owaki, T., Kamiya, S., Matsunaga, T., Shimoda, K., Kodama, H., Hayashi, R., Abe, T., Harada, Y. P., Shimonaka, M., Yajima, H., Terada, H., Fukai, F. (2009). VLA-5-mediated Adhesion to Fibronectin Accelerates Hemin-stimulated Erythroid Differentiation of K562 Cells through Induction of VLA-4 Expression. J. Biol. Chem. 284: 19817-19825 [Abstract] [Full Text]
Drumea-Mirancea, M., Wessels, J. T., Muller, C. A., Essl, M., Eble, J. A., Tolosa, E., Koch, M., Reinhardt, D. P., Sixt, M., Sorokin, L., Stierhof, Y.-D., Schwarz, H., Klein, G. (2006). Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules. J. Cell Sci. 119: 1396-1405 [Abstract] [Full Text]
Garcion, E., Halilagic, A., Faissner, A., ffrench-Constant, C. (2004). Generation of an environmental niche for neural stem cell development by the extracellular matrix molecule tenascin C. Development 131: 3423-3432 [Abstract] [Full Text]
Suetterlin, R., Baschong, W., Laeng, R. H. (2004). Immunofluorescence and Confocal Laser Scanning Microscopy of Chronic Myeloproliferative Disorders on Archival Formaldehyde-fixed Bone Marrow. J. Histochem. Cytochem. 52: 347-354 [Abstract] [Full Text]
Puch, S, Armeanu, S, Kibler, C, Johnson, K., Muller, C., Wheelock, M., Klein, G (2001). N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. J. Cell Sci. 114: 1567-1577 [Abstract]
Siler, U., Seiffert, M., Puch, S., Richards, A., Torok-Storb, B., Muller, C. A., Sorokin, L., Klein, G. (2000). Characterization and functional analysis of laminin isoforms in human bone marrow. Blood 96: 4194-4203 [Abstract] [Full Text]
Yokota, T., Oritani, K., Takahashi, I., Ishikawa, J., Matsuyama, A., Ouchi, N., Kihara, S., Funahashi, T., Tenner, A. J., Tomiyama, Y., Matsuzawa, Y. (2000). Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood 96: 1723-1732 [Abstract] [Full Text]
Mackie, E., Tucker, R. (1999). The tenascin-C knockout revisited. J. Cell Sci. 112: 3847-3853 [Abstract]
Ohta, M., Sakai, T., Saga, Y., Aizawa, S.-i., Saito, M. (1998). Suppression of Hematopoietic Activity in Tenascin-C-Deficient Mice. Blood 91: 4074-4083 [Abstract] [Full Text]
Kuru, L., Parkar, M.H., Griffiths, G.S., Newman, H.N., Olsen, I. (1998). Flow Cytometry Analysis of Gingival and Periodontal Ligament Cells. JDR 77: 555-564 [Abstract]
Clark, R. A., Erickson, H. P., Springer, T. A. (1997). Tenascin Supports Lymphocyte Rolling. JCB 137: 755-765 [Abstract] [Full Text]
Talts, J., Weller, A, Timpl, R, Ekblom, M, Ekblom, P (1995). Regulation of mesenchymal extracellular matrix protein synthesis by transforming growth factor-beta and glucocorticoids in tumor stroma. J. Cell Sci. 108: 2153-2162 [Abstract]