Cytoplasmic dynein plays a role in mammalian mitotic spindle formation

doi:10.1083/jcb.123.4.849

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Cytoplasmic dynein plays a role in mammalian mitotic spindle formation

EA Vaisberg, MP Koonce and JR McIntosh
Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder 80309-0347.

The formation and functioning of a mitotic spindle depends not only on the assembly/disassembly of microtubules but also on the action of motor enzymes. Cytoplasmic dynein has been localized to spindles, but whether or how it functions in mitotic processes is not yet known. We have cloned and expressed DNA fragments that encode the putative ATP- hydrolytic sites of the cytoplasmic dynein heavy chain from HeLa cells and from Dictyostelium. Monospecific antibodies have been raised to the resulting polypeptides, and these inhibit dynein motor activity in vitro. Their injection into mitotic mammalian cells blocks the formation of spindles in prophase or during recovery from nocodazole treatment at later stages of mitosis. Cells become arrested with unseparated centrosomes and form monopolar spindles. The injected antibodies have no detectable effect on chromosome attachment to a bipolar spindle or on motions during anaphase. These data suggest that cytoplasmic dynein plays a unique and important role in the initial events of bipolar spindle formation, while any later roles that it may play are redundant. Possible mechanisms of dynein's involvement in mitosis are discussed.
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Dohner, K., Wolfstein, A., Prank, U., Echeverri, C., Dujardin, D., Vallee, R., Sodeik, B. (2002). Function of Dynein and Dynactin in Herpes Simplex Virus Capsid Transport. Mol. Biol. Cell 13: 2795-2809 [Abstract] [Full Text]
Grissom, P. M., Vaisberg, E. A., McIntosh, J. R. (2002). Identification of a Novel Light Intermediate Chain (D2LIC) for Mammalian Cytoplasmic Dynein 2. Mol. Biol. Cell 13: 817-829 [Abstract] [Full Text]
Yoder, J. H., Han, M. (2001). Cytoplasmic Dynein Light Intermediate Chain Is Required for Discrete Aspects of Mitosis in Caenorhabditis elegans. Mol. Biol. Cell 12: 2921-2933 [Abstract] [Full Text]
Habermann, A, Schroer, T., Griffiths, G, Burkhardt, J. (2001). Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles. J. Cell Sci. 114: 229-240 [Abstract]
Schuyler, S., Pellman, D (2001). Search, capture and signal: games microtubules and centrosomes play. J. Cell Sci. 114: 247-255 [Abstract]
Mayor, T., Stierhof, Y.-D., Tanaka, K., Fry, A. M., Nigg, E. A. (2000). The Centrosomal Protein C-Nap1 Is Required for Cell Cycle-Regulated Centrosome Cohesion. JCB 151: 837-846 [Abstract] [Full Text]
King, J. M., Hays, T. S., Nicklas, R. B. (2000). Dynein Is a Transient Kinetochore Component Whose Binding Is Regulated by Microtubule Attachment, Not Tension. JCB 151: 739-748 [Abstract] [Full Text]
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Gonczy, P., Schnabel, H., Kaletta, T., Amores, A. D., Hyman, T., Schnabel, R. (1999). Dissection of Cell Division Processes in the One Cell Stage Caenorhabditis elegans Embryo by Mutational Analysis. JCB 144: 927-946 [Abstract] [Full Text]
Lee, S., Wisniewski, J. C., Dentler, W. L., Asai, D. J. (1999). Gene Knockouts Reveal Separate Functions for Two Cytoplasmic Dyneins in Tetrahymena thermophila. Mol. Biol. Cell 10: 771-784 [Abstract] [Full Text]
Roghi, C, Allan, V. (1999). Dynamic association of cytoplasmic dynein heavy chain 1a with the Golgi apparatus and intermediate compartment. J. Cell Sci. 112: 4673-4685 [Abstract]
Palazzo, R., Vaisberg, E., Weiss, D., Kuznetsov, S., Steffen, W (1999). Dynein is required for spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes. J. Cell Sci. 112: 1291-1302 [Abstract]
Straight, A. F., Sedat, J. W., Murray, A. W. (1998). Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast. JCB 143: 687-694 [Abstract] [Full Text]
Nurminsky, D. I., Nurminskaya, M. V., Benevolenskaya, E. V., Shevelyov, Y. Y., Hartl, D. L., Gvozdev, V. A. (1998). Cytoplasmic Dynein Intermediate-Chain Isoforms with Different Targeting Properties Created by Tissue-Specific Alternative Splicing. Mol. Cell. Biol. 18: 6816-6825 [Abstract] [Full Text]
Karki, S., LaMonte, B., Holzbaur, E. L.F. (1998). Characterization of the p22 Subunit of Dynactin Reveals the Localization of Cytoplasmic Dynein and Dynactin to the Midbody of Dividing Cells. JCB 142: 1023-1034 [Abstract] [Full Text]
Starr, D. A., Williams, B. C., Hays, T. S., Goldberg, M. L. (1998). ZW10 Helps Recruit Dynactin and Dynein to the Kinetochore. JCB 142: 763-774 [Abstract] [Full Text]
Kahana, J. A., Schlenstedt, G., Evanchuk, D. M., Geiser, J. R., Hoyt, M. A., Silver, P. A. (1998). The Yeast Dynactin Complex Is Involved in Partitioning the Mitotic Spindle between Mother and Daughter Cells during Anaphase B. Mol. Biol. Cell 9: 1741-1756 [Abstract] [Full Text]
Efimov, V. P., Morris, N. R. (1998). A Screen for Dynein Synthetic Lethals in Aspergillus nidulans Identifies Spindle Assembly Checkpoint Genes and Other Genes Involved in Mitosis. Genetics 149: 101-116 [Abstract] [Full Text]
Harada, A., Takei, Y., Kanai, Y., Tanaka, Y., Nonaka, S., Hirokawa, N. (1998). Golgi Vesiculation and Lysosome Dispersion in Cells Lacking Cytoplasmic Dynein. JCB 141: 51-59 [Abstract] [Full Text]
Marples, D., Schroer, T. A., Ahrens, N., Taylor, A., Knepper, M. A., Nielsen, S. (1998). Dynein and dynactin colocalize with AQP2 water channels in intracellular vesicles from kidney collecting duct. Am. J. Physiol. Renal Physiol. 274: F384-F394 [Abstract] [Full Text]
Criswell, P. S., Asai, D. J. (1998). Evidence for Four Cytoplasmic Dynein Heavy Chain Isoforms in Rat Testis. Mol. Biol. Cell 9: 237-247 [Abstract] [Full Text]
Inoue, S, Yoder, O., Turgeon, B., Aist, J. (1998). A cytoplasmic dynein required for mitotic aster formation in vivo. J. Cell Sci. 111: 2607-2614 [Abstract]
Compton, D. (1998). Focusing on spindle poles. J. Cell Sci. 111: 1477-1481 [Abstract]
Inoue, S, Turgeon, B., Yoder, O., Aist, J. (1998). Role of fungal dynein in hyphal growth, microtubule organization, spindle pole body motility and nuclear migration. J. Cell Sci. 111: 1555-1566 [Abstract]
Schaar, B.T., Chan, G.K.T., Maddox, P., Salmon, E.D., Yen, T.J. (1997). CENP-E Function at Kinetochores Is Essential for Chromosome Alignment. JCB 139: 1373-1382 [Abstract] [Full Text]
Yao, X., Anderson, K. L., Cleveland, D. W. (1997). The Microtubule-dependent Motor Centromere-associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules. JCB 139: 435-447 [Abstract] [Full Text]
Steffen, W., Karki, S., Vaughan, K. T., Vallee, R. B., Holzbaur, E. L.F., Weiss, D. G., Kuznetsov, S. A. (1997). The Involvement of the Intermediate Chain of Cytoplasmic Dynein in Binding the Motor Complex to Membranous Organelles of Xenopus Oocytes. Mol. Biol. Cell 8: 2077-2088 [Abstract] [Full Text]
Gaglio, T., Dionne, M. A., Compton, D. A. (1997). Mitotic Spindle Poles are Organized by Structural and Motor Proteins in Addition to Centrosomes. JCB 138: 1055-1066 [Abstract] [Full Text]
Sharp, D. J., Yu, W., Ferhat, L., Kuriyama, R., Rueger, D. C., Baas, P. W. (1997). Identification of a Microtubule-associated Motor Protein Essential for Dendritic Differentiation. JCB 138: 833-843 [Abstract] [Full Text]
Carminati, J. L., Stearns, T. (1997). Microtubules Orient the Mitotic Spindle in Yeast through Dynein-dependent Interactions with the Cell Cortex. JCB 138: 629-641 [Abstract] [Full Text]
Heald, R., Tournebize, R., Habermann, A., Karsenti, E., Hyman, A. (1997). Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization. JCB 138: 615-628 [Abstract] [Full Text]
Koonce, M. P. (1997). Identification of a Microtubule-binding Domain in a Cytoplasmic Dynein Heavy Chain. J. Biol. Chem. 272: 19714-19718 [Abstract] [Full Text]
Blangy, A., Arnaud, L., Nigg, E. A. (1997). Phosphorylation by p34cdc2 Protein Kinase Regulates Binding of the Kinesin-related Motor HsEg5 to the Dynactin Subunit p150Glued. J. Biol. Chem. 272: 19418-19424 [Abstract] [Full Text]
Blocker, A., Severin, F. F., Burkhardt, J. K., Bingham, J. B., Yu, H., Olivo, J.-C., Schroer, T. A., Hyman, A. A., Griffiths, G. (1997). Molecular Requirements for Bi-directional Movement of Phagosomes Along Microtubules. JCB 137: 113-129 [Abstract] [Full Text]
Sodeik, B., Ebersold, M. W., Helenius, A. (1997). Microtubule-mediated Transport of Incoming Herpes Simplex Virus 1 Capsids to the Nucleus. JCB 136: 1007-1021 [Abstract] [Full Text]
Walczak, C. E., Verma, S., Mitchison, T. J. (1997). XCTK2: A Kinesin-related Protein That Promotes Mitotic Spindle Assembly in Xenopus laevis Egg Extracts. JCB 136: 859-870 [Abstract] [Full Text]
Morris, S. M., Anaya, P., Xiang, X., Morris, N. R., May, G. S., Yu-Lee, L.-y. (1997). A Prolactin-Inducible T Cell Gene Product Is Structurally Similar to the Aspergillus nidulans Nuclear Movement Protein NUDC. Mol. Endocrinol. 11: 229-236 [Abstract] [Full Text]
Blocker, A., Severin, F. F., Habermann, A., Hyman, A. A., Griffiths, G., Burkhardt, J. K. (1996). Microtubule-associated Protein-dependent Binding of Phagosomes to Microtubules. J. Biol. Chem. 271: 3803-3811 [Abstract] [Full Text]
Waterman-Storer, C. M., Holzbaur, E. L. F. (1996). The Product of the Drosophila Gene, Glued, Is the Functional Homologue of the p150[IMAGE]Component of the Vertebrate Dynactin Complex. J. Biol. Chem. 271: 1153-1159 [Abstract] [Full Text]
Brown, K., Wood, K., Cleveland, D. (1996). The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. J. Cell Sci. 109: 961-969 [Abstract]
Masuda, H, Shibata, T (1996). Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body. J. Cell Sci. 109: 165-177 [Abstract]
Karki, S., Holzbaur, E. L. F. (1995). Affinity Chromatography Demonstrates a Direct Binding between Cytoplasmic Dynein and the Dynactin Complex. J. Biol. Chem. 270: 28806-28811 [Abstract] [Full Text]
Tanaka, Y, Zhang, Z, Hirokawa, N (1995). Identification and molecular evolution of new dynein-like protein sequences in rat brain. J. Cell Sci. 108: 1883-1893 [Abstract]
Moscatelli, A, Del Casino, C, Lozzi, L, Cai, G, Scali, M, Tiezzi, A, Cresti, M (1995). High molecular weight polypeptides related to dynein heavy chains in Nicotiana tabacum pollen tubes. J. Cell Sci. 108: 1117-1125 [Abstract]
Vallee, R.B., Vaughan, K.T., Escheverri, C.J. (1995). Targeting of Cytoplasmic Dynein to Membranous Organelles and Kinetochores via Dynactin. Cold Spring Harb Symp Quant Biol 60: 803-811 [Abstract]
Xiang, X., Osmani, A.H., Osmani, S.A., Roghi, C.H., Willins, D.A., Beckwith, S., Goldman, G., Chiu, Y., Xin, M., Liu, B., Morris, N.R. (1995). Analysis of Nuclear Migration in Aspergillus nidulans. Cold Spring Harb Symp Quant Biol 60: 813-819 [Abstract]
Hays, T., Porter, M., McGrail, M, Grissom, P, Gosch, P, Fuller, M., McIntosh, J. (1994). A cytoplasmic dynein motor in Drosophila: identification and localization during embryogenesis. J. Cell Sci. 107: 1557-1569 [Abstract]
Asai, D., Beckwith, S., Kandl, K., Keating, H., Tjandra, H, Forney, J. (1994). The dynein genes of Paramecium tetraurelia. Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms. J. Cell Sci. 107: 839-847 [Abstract]
Crespo, N. C., Ohkanda, J., Yen, T. J., Hamilton, A. D., Sebti, S. M. (2001). The Farnesyltransferase Inhibitor, FTI-2153, Blocks Bipolar Spindle Formation and Chromosome Alignment and Causes Prometaphase Accumulation during Mitosis of Human Lung Cancer Cells. J. Biol. Chem. 276: 16161-16167 [Abstract] [Full Text]
Eichenmuller, B., Everley, P., Palange, J., Lepley, D., Suprenant, K. A. (2002). The Human EMAP-like Protein-70 (ELP70) Is a Microtubule Destabilizer That Localizes to the Mitotic Apparatus. J. Biol. Chem. 277: 1301-1309 [Abstract] [Full Text]
Howell, B.J., McEwen, B.F., Canman, J.C., Hoffman, D.B., Farrar, E.M., Rieder, C.L., Salmon, E.D. (2001). Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation. JCB 155: 1159-1172 [Abstract] [Full Text]