Identification of sequences required for the efficient localization of the focal adhesion kinase, pp125FAK, to cellular focal adhesions

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Identification of sequences required for the efficient localization of the focal adhesion kinase, pp125FAK, to cellular focal adhesions

JD Hildebrand, MD Schaller and JT Parsons
Department of Microbiology, University of Virginia, Charlottesville 22908.

The integrin family of heterodimeric cell surface receptors play critical roles in multiple biological processes by mediating cellular adhesion to the extracellular matrix (ECM). Adhesion triggers intracellular signaling cascades, including tyrosine phosphorylation and elevation of [Ca2+]i. The Focal Adhesion Kinase (FAK or pp125FAK), a protein tyrosine kinase that colocalizes with integrins in cellular focal adhesions, is a prime candidate for a mediator of integrin signaling events. Here we report an analysis of the domain structure of FAK in which we have identified a contiguous stretch of 159 amino acids within the COOH terminus essential for correct subcellular localization. When placed in the context of an unrelated cytosolic protein, this Focal Adhesion Targeting (FAT) sequence functions to efficiently mediate the focal adhesion localization of this fusion protein. Furthermore, this analysis suggests that pp125FAK cannot be activated oncogenically by mutation. This result could be explained if pp125FK either exhibits a narrow substrate specificity or is diametrically opposed by cellular phosphatases or other cellular processes.
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Parsons, J. T. (2003). Focal adhesion kinase: the first ten years. J. Cell Sci. 116: 1409-1416 [Abstract] [Full Text]
Toutant, M., Costa, A., Studler, J.-M., Kadare, G., Carnaud, M., Girault, J.-A. (2002). Alternative Splicing Controls the Mechanisms of FAK Autophosphorylation. Mol. Cell. Biol. 22: 7731-7743 [Abstract] [Full Text]
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Liu, G., Guibao, C. D., Zheng, J. (2002). Structural Insight into the Mechanisms of Targeting and Signaling of Focal Adhesion Kinase. Mol. Cell. Biol. 22: 2751-2760 [Abstract] [Full Text]
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Li, L., Okura, M., Imamoto, A. (2002). Focal Adhesions Require Catalytic Activity of Src Family Kinases To Mediate Integrin-Matrix Adhesion. Mol. Cell. Biol. 22: 1203-1217 [Abstract] [Full Text]
Stupack, D. G., Puente, X. S., Boutsaboualoy, S., Storgard, C. M., Cheresh, D. A. (2001). Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins. JCB 155: 459-470 [Abstract] [Full Text]
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Jones, G., Machado, J. Jr., Merlo, A. (2001). Loss of Focal Adhesion Kinase (FAK) Inhibits Epidermal Growth Factor Receptor-dependent Migration and Induces Aggregation of NH2-Terminal FAK in the Nuclei of Apoptotic Glioblastoma Cells. Cancer Res. 61: 4978-4981 [Abstract] [Full Text]
Ren, X.-R., Du, Q.-S., Huang, Y.-Z., Ao, S.-Z., Mei, L., Xiong, W.-C. (2001). Regulation of Cdc42 Gtpase by Proline-Rich Tyrosine Kinase 2 Interacting with Psgap, a Novel Pleckstrin Homology and Src Homology 3 Domain Containing Rhogap Protein. JCB 152: 971-984 [Abstract] [Full Text]
Klingbeil, C. K., Hauck, C. R., Hsia, D. A., Jones, K.C., Reider, S. R., Schlaepfer, D. D. (2001). Targeting Pyk2 to {beta}1-Integrin-Containing Focal Contacts Rescues Fibronectin-Stimulated Signaling and Haptotactic Motility Defects of Focal Adhesion Kinase-Null Cells. JCB 152: 97-110 [Abstract] [Full Text]
Fincham, V. J., Brunton, V. G., Frame, M. C. (2000). The SH3 Domain Directs Acto-Myosin-Dependent Targeting of v-Src to Focal Adhesions via Phosphatidylinositol 3-Kinase. Mol. Cell. Biol. 20: 6518-6536 [Abstract] [Full Text]
Cooley, M. A., Broome, J. M., Ohngemach, C., Romer, L. H., Schaller, M. D. (2000). Paxillin Binding Is Not the Sole Determinant of Focal Adhesion Localization or Dominant-Negative Activity of Focal Adhesion Kinase/Focal Adhesion Kinase-related Nonkinase. Mol. Biol. Cell 11: 3247-3263 [Abstract] [Full Text]
Xiong, W., Macklem, M, Parsons, J. (2000). Expression and characterization of splice variants of PYK2, a focal adhesion kinase-related protein. J. Cell Sci. 111: 1981-1991 [Abstract]
Chen, L.-M., Bailey, D., Fernandez-Valle, C. (2000). Association of beta 1 Integrin with Focal Adhesion Kinase and Paxillin in Differentiating Schwann Cells. J. Neurosci. 20: 3776-3784 [Abstract] [Full Text]
Almeida, E. A.C., Ilic, D., Han, Q., Hauck, C. R., Jin, F., Kawakatsu, H., Schlaepfer, D. D., Damsky, C. H. (2000). Matrix Survival Signaling: From Fibronectin via Focal Adhesion Kinase to C-Jun Nh2-Terminal Kinase. JCB 149: 741-754 [Abstract] [Full Text]
Zhao, J, Zheng, C, Guan, J (2000). Pyk2 and FAK differentially regulate progression of the cell cycle. J. Cell Sci. 113: 3063-3072 [Abstract]
Thomas, J. W., Cooley, M. A., Broome, J. M., Salgia, R., Griffin, J. D., Lombardo, C. R., Schaller, M. D. (1999). The Role of Focal Adhesion Kinase Binding in the Regulation of Tyrosine Phosphorylation of Paxillin. J. Biol. Chem. 274: 36684-36692 [Abstract] [Full Text]
Palmer, R. H., Fessler, L. I., Edeen, P. T., Madigan, S. J., McKeown, M., Hunter, T. (1999). DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase. J. Biol. Chem. 274: 35621-35629 [Abstract] [Full Text]
Carragher, N. O., Levkau, B., Ross, R., Raines, E. W. (1999). Degraded Collagen Fragments Promote Rapid Disassembly of Smooth Muscle Focal Adhesions That Correlates with Cleavage of Pp125FAK, Paxillin, and Talin. JCB 147: 619-630 [Abstract] [Full Text]
Fujimoto, J., Sawamoto, K., Okabe, M., Takagi, Y., Tezuka, T., Yoshikawa, S., Ryo, H., Okano, H., Yamamoto, T. (1999). Cloning and Characterization of Dfak56, a Homolog of Focal Adhesion Kinase, in Drosophila melanogaster. J. Biol. Chem. 274: 29196-29201 [Abstract] [Full Text]
Schaller, M. D., Hildebrand, J. D., Parsons, J. T. (1999). Complex Formation with Focal Adhesion Kinase: A Mechanism to Regulate Activity and Subcellular Localization of Src Kinases. Mol. Biol. Cell 10: 3489-3505 [Abstract] [Full Text]
Nolan, K., Lacoste, J., Parsons, J. T. (1999). Regulated Expression of Focal Adhesion Kinase-Related Nonkinase, the Autonomously Expressed C-Terminal Domain of Focal Adhesion Kinase. Mol. Cell. Biol. 19: 6120-6129 [Abstract] [Full Text]
Shen, Y., Schaller, M. D. (1999). Focal Adhesion Targeting: The Critical Determinant of FAK Regulation and Substrate Phosphorylation. Mol. Biol. Cell 10: 2507-2518 [Abstract] [Full Text]
van de Water, B., Nagelkerke, J. F., Stevens, J. L. (1999). Dephosphorylation of Focal Adhesion Kinase (FAK) and Loss of Focal Contacts Precede Caspase-mediated Cleavage of FAK during Apoptosis in Renal Epithelial Cells. J. Biol. Chem. 274: 13328-13337 [Abstract] [Full Text]
Li, X., Dy, R. C., Cance, W. G., Graves, L. M., Earp, H. S. (1999). Interactions between Two Cytoskeleton-associated Tyrosine Kinases: Calcium-dependent Tyrosine Kinase and Focal Adhesion Tyrosine Kinase. J. Biol. Chem. 274: 8917-8924 [Abstract] [Full Text]
YEAMAN, C., GRINDSTAFF, K. K., NELSON, W. J. (1999). New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79: 73-98 [Abstract] [Full Text]
Zhao, J.-H., Reiske, H., Guan, J.-L. (1998). Regulation of the Cell Cycle by Focal Adhesion Kinase. JCB 143: 1997-2008 [Abstract] [Full Text]
Lebrun, P., Mothe-Satney, I., Delahaye, L., Van Obberghen, E., Baron, V. (1998). Insulin Receptor Substrate-1 as a Signaling Molecule for Focal Adhesion Kinase pp125FAK and pp60src. J. Biol. Chem. 273: 32244-32253 [Abstract] [Full Text]
Alrutz, M. A., Isberg, R. R. (1998). Involvement of focal adhesion kinase in invasin-mediated uptake. Proc. Natl. Acad. Sci. USA 95: 13658-13663 [Abstract] [Full Text]
Ilic, D., Almeida, E. A.C., Schlaepfer, D. D., Dazin, P., Aizawa, S., Damsky, C. H. (1998). Extracellular Matrix Survival Signals Transduced by Focal Adhesion Kinase Suppress p53-mediated Apoptosis. JCB 143: 547-560 [Abstract] [Full Text]
Aplin, A. E., Howe, A., Alahari, S. K., Juliano, R. L. (1998). Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins. Pharmacol. Rev. 50: 197-264 [Abstract] [Full Text]
Baron, V., Calleja, V., Ferrari, P., Alengrin, F., Van Obberghen, E. (1998). p125Fak Focal Adhesion Kinase Is a Substrate for the Insulin and Insulin-like Growth Factor-I Tyrosine Kinase Receptors. J. Biol. Chem. 273: 7162-7168 [Abstract] [Full Text]
Shen, Y., Schneider, G., Cloutier, J.-F., Veillette, A., Schaller, M. D. (1998). Direct Association of Protein-tyrosine Phosphatase PTP-PEST with Paxillin. J. Biol. Chem. 273: 6474-6481 [Abstract] [Full Text]
Ribon, V., Herrera, R., Kay, B. K., Saltiel, A. R. (1998). A Role for CAP, a Novel, Multifunctional Src Homology 3�Domain-containing Protein in Formation of Actin Stress Fibers and Focal Adhesions. J. Biol. Chem. 273: 4073-4080 [Abstract] [Full Text]
Zheng, C., Xing, Z., Bian, Z. C., Guo, C., Akbay, A., Warner, L., Guan, J.-L. (1998). Differential Regulation of Pyk2 and Focal Adhesion Kinase (FAK). THE C-TERMINAL DOMAIN OF FAK CONFERS RESPONSE TO CELL ADHESION. J. Biol. Chem. 273: 2384-2389 [Abstract] [Full Text]
Xiong, W.-c., Parsons, J. T. (1997). Induction of Apoptosis after Expression of PYK2, a Tyrosine Kinase Structurally Related to Focal Adhesion Kinase. JCB 139: 529-539 [Abstract] [Full Text]
Schaller, M. D., Sasaki, T. (1997). Differential Signaling by the Focal Adhesion Kinase and Cell Adhesion Kinase beta. J. Biol. Chem. 272: 25319-25325 [Abstract] [Full Text]
Lyman, S., Gilmore, A., Burridge, K., Gidwitz, S., White II, G. C. (1997). Integrin-mediated Activation of Focal Adhesion Kinase Is Independent of Focal Adhesion Formation or Integrin Activation. STUDIES WITH ACTIVATED AND INHIBITORY beta 3 CYTOPLASMIC DOMAIN MUTANTS. J. Biol. Chem. 272: 22538-22547 [Abstract] [Full Text]
Manie, S. N., Astier, A., Haghayeghi, N., Canty, T., Druker, B. J., Hirai, H., Freedman, A. S. (1997). Regulation of Integrin-mediated p130Cas Tyrosine Phosphorylation in Human B Cells. A ROLE FOR p59Fyn AND SHP2. J. Biol. Chem. 272: 15636-15641 [Abstract] [Full Text]
Li, X., Earp, H. S. (1997). Paxillin Is Tyrosine-phosphorylated by and Preferentially Associates with the Calcium-dependent Tyrosine Kinase in Rat Liver Epithelial Cells. J. Biol. Chem. 272: 14341-14348 [Abstract] [Full Text]
Schlaepfer, D. D., Hunter, T. (1997). Focal Adhesion Kinase Overexpression Enhances Ras-dependent Integrin Signaling to ERK2/Mitogen-activated Protein Kinase through Interactions with and Activation of c-Src. J. Biol. Chem. 272: 13189-13195 [Abstract] [Full Text]
Beggs, H. E., Baragona, S. C., Hemperly, J. J., Maness, P. F. (1997). NCAM140 Interacts with the Focal Adhesion Kinase p125fak and the SRC-related Tyrosine Kinase p59fyn. J. Biol. Chem. 272: 8310-8319 [Abstract] [Full Text]
Lin, T. H., Aplin, A. E., Shen, Y., Chen, Q., Schaller, M., Romer, L., Aukhil, I., Juliano, R.L. (1997). Integrin-mediated Activation of MAP Kinase Is Independent of FAK: Evidence for Dual Integrin Signaling Pathways in Fibroblasts. JCB 136: 1385-1395 [Abstract] [Full Text]
Couchman, G. M., Bentley, R., Tsao, M.-S., Raszmann, K., McLachlan, J. A., Walmer, D. K. (1997). p53lyn and p56lyn: A New Signaling Pathway in Human Endometrium and Endometrial Adenocarcinomas. Reproductive Sciences 4: 103-109 [Abstract]
Polte, T. R., Hanks, S. K. (1997). Complexes of Focal Adhesion Kinase (FAK) and Crk-associated Substrate (p130Cas) Are Elevated in Cytoskeleton-associated Fractions following Adhesion and Src Transformation. REQUIREMENTS FOR Src KINASE ACTIVITY AND FAK PROLINE-RICH MOTIFS. J. Biol. Chem. 272: 5501-5509 [Abstract] [Full Text]
Zong, C. S., Chan, J. L.-K., Yang, S.-K., Wang, L.-H. (1997). Mutations of Ros Differentially Effecting Signal Transduction Pathways Leading to Cell Growth Versus Transformation. J. Biol. Chem. 272: 1500-1506 [Abstract] [Full Text]
Ayalon, O, Geiger, B (1997). Cyclic changes in the organization of cell adhesions and the associated cytoskeleton, induced by stimulation of tyrosine phosphorylation in bovine aortic endothelial cells. J. Cell Sci. 110: 547-556 [Abstract]
Konstantopoulos, N., Clark, S. (1996). Reduced Cell Attachment and Phosphorylation of Focal Adhesion Kinase Associated with Expression of a Mutant Insulin Receptor. J. Biol. Chem. 271: 28960-28968 [Abstract] [Full Text]
Derkinderen, P., Toutant, M., Burgaya, F., Le Bert, M., Siciliano, J. C., de Franciscis, V., Gelman, M., Girault, J.-A. (1996). Regulation of a Neuronal Form of Focal Adhesion Kinase by Anandamide. Science 273: 1719-1722 [Abstract] [Full Text]
Harte, M. T., Hildebrand, J. D., Burnham, M. R., Bouton, A. H., Parsons, J. T. (1996). p130Cas, a Substrate Associated with v-Src and v-Crk, Localizes to Focal Adhesions and Binds to Focal Adhesion Kinase. J. Biol. Chem. 271: 13649-13655 [Abstract] [Full Text]
Cary, L., Chang, J., Guan, J. (1996). Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J. Cell Sci. 109: 1787-1794 [Abstract]
Avraham, S., London, R., Fu, Y., Ota, S., Hiregowdara, D., Li, J., Jiang, S., Pasztor, L. M., White, R. A., Groopman, J. E., Avraham, H. (1995). Identification and Characterization of a Novel Related Adhesion Focal Tyrosine Kinase (RAFTK) from Megakaryocytes and Brain. J. Biol. Chem. 270: 27742-27751 [Abstract] [Full Text]
Sasaki, H., Nagura, K., Ishino, M., Tobioka, H., Kotani, K., Sasaki, T. (1995). Cloning and Characterization of Cell Adhesion Kinase beta, a Novel Protein-tyrosine Kinase of the Focal Adhesion Kinase Subfamily. J. Biol. Chem. 270: 21206-21219 [Abstract] [Full Text]
Grant, S G, Karl, K A, Kiebler, M A, Kandel, E R (1995). Focal adhesion kinase in the brain: novel subcellular localization and specific regulation by Fyn tyrosine kinase in mutant mice.. Genes Dev. 9: 1909-1921 [Abstract]
Chen, H.-C., Appeddu, P. A., Parsons, J. T., Hildebrand, J. D., Schaller, M. D., Guan, J.-L. (1995). Interaction of Focal Adhesion Kinase with Cytoskeletal Protein Talin. J. Biol. Chem. 270: 16995-16999 [Abstract] [Full Text]
Hamawy, M. M., Minoguchi, K., Swaim, W. D., Mergenhagen, S. E., Siraganian, R. P. (1995). A 77-kDa Protein Associates with pp125[IMAGE] in Mast Cells and Becomes Tyrosine-phosphorylated by High Affinity IgE Receptor Aggregation. J. Biol. Chem. 270: 12305-12309 [Abstract] [Full Text]
Clark, E., Brugge, J. (1995). Integrins and signal transduction pathways: the road taken. Science 268: 233-239 [Abstract]
Pillay, T. S., Sasaoka, T., Olefsky, J. M. (1995). Insulin Stimulates the Tyrosine Dephosphorylation of pp125 Focal Adhesion Kinase. J. Biol. Chem. 270: 991-994 [Abstract] [Full Text]
Leong, L, Hughes, P., Schwartz, M., Ginsberg, M., Shattil, S. (1995). Integrin signaling: roles for the cytoplasmic tails of alpha IIb beta 3 in the tyrosine phosphorylation of pp125FAK. J. Cell Sci. 108: 3817-3825 [Abstract]
Wilson, L, Carrier, M., Kellie, S (1995). pp125FAK tyrosine kinase activity is not required for the assembly of F-actin stress fibres and focal adhesions in cultured mouse aortic smooth muscle cells. J. Cell Sci. 108: 2381-2391 [Abstract]
Turner, C., Miller, J. (1994). Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: identification of a vinculin and pp125Fak-binding region. J. Cell Sci. 107: 1583-1591 [Abstract]
Liu, S., Ginsberg, M. H. (2000). Paxillin Binding to a Conserved Sequence Motif in the alpha 4 Integrin Cytoplasmic Domain. J. Biol. Chem. 275: 22736-22742 [Abstract] [Full Text]
Vial, D., Okazaki, H., Siraganian, R. P. (2000). The NH2-terminal Region of Focal Adhesion Kinase Reconstitutes High Affinity IgE Receptor-induced Secretion in Mast Cells. J. Biol. Chem. 275: 28269-28275 [Abstract] [Full Text]
Litvak, V., Tian, D., Shaul, Y. D., Lev, S. (2000). Targeting of PYK2 to Focal Adhesions as a Cellular Mechanism for Convergence between Integrins and G Protein-coupled Receptor Signaling Cascades. J. Biol. Chem. 275: 32736-32746 [Abstract] [Full Text]
Hauck, C. R., Hsia, D. A., Schlaepfer, D. D. (2000). Focal Adhesion Kinase Facilitates Platelet-derived Growth Factor-BB-stimulated ERK2 Activation Required for Chemotaxis Migration of Vascular Smooth Muscle Cells. J. Biol. Chem. 275: 41092-41099 [Abstract] [Full Text]
Suzuki-Inoue, K., Ozaki, Y., Kainoh, M., Shin, Y., Wu, Y., Yatomi, Y., Ohmori, T., Tanaka, T., Satoh, K., Morita, T. (2001). Rhodocytin Induces Platelet Aggregation by Interacting with Glycoprotein Ia/IIa (GPIa/IIa, Integrin alpha 2beta 1). INVOLVEMENT OF GPIa/IIa-ASSOCIATED Src AND PROTEIN TYROSINE PHOSPHORYLATION. J. Biol. Chem. 276: 1643-1652 [Abstract] [Full Text]
Xie, B., Zhao, J., Kitagawa, M., Durbin, J., Madri, J. A., Guan, J.-L., Fu, X.-Y. (2001). Focal Adhesion Kinase Activates Stat1 in Integrin-mediated Cell Migration and Adhesion. J. Biol. Chem. 276: 19512-19523 [Abstract] [Full Text]
Poullet, P., Gautreau, A., Kadare, G., Girault, J.-A., Louvard, D., Arpin, M. (2001). Ezrin Interacts with Focal Adhesion Kinase and Induces Its Activation Independently of Cell-matrix Adhesion. J. Biol. Chem. 276: 37686-37691 [Abstract] [Full Text]
McLean, G. W., Fincham, V. J., Frame, M. C. (2000). v-Src Induces Tyrosine Phosphorylation of Focal Adhesion Kinase Independently of Tyrosine 397 and Formation of a Complex with Src. J. Biol. Chem. 275: 23333-23339 [Abstract] [Full Text]