Olfactory receptors are displayed on dog mature sperm cells

doi:10.1083/jcb.123.6.1441

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 2779K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Vanderhaeghen, P.
	Articles by Parmentier, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Vanderhaeghen, P.
	Articles by Parmentier, M.


Social Bookmarking

	What's this?

ARTICLES

Olfactory receptors are displayed on dog mature sperm cells

P Vanderhaeghen, S Schurmans, G Vassart and M Parmentier
Institute of Interdisciplinary Research, Universite Libre de Bruxelles, Belgium.

Olfactory receptors constitute a huge family of structurally related G protein-coupled receptors, with up to a thousand members expected. We have shown previously that genes belonging to this family were expressed in the male germ line from both dog and human. The functional significance of this unexpected site of expression was further investigated in the present study. We demonstrate that a few dog genes representative of various subfamilies of olfactory receptors are expressed essentially in testis, with little or no expression in olfactory mucosa. Other randomly selected members of the family show the expected site of expression, restricted to the olfactory system. Antibodies were generated against the deduced amino acid sequence of the most abundantly expressed olfactory receptor gene in dog testis. The purified serum was able to detect the gene product (DTMT receptor) in late round and elongated spermatids, as well as in the cytoplasmic droplet that characterizes the maturation of dog sperm cells, and on the tail midpiece of mature spermatozoa. Western blotting further confirmed the presence of a 40-kD immunoreactive protein in the membrane of mature sperm cells. Altogether , these results demonstrate that the main expression site of a subset of the large olfactory receptor gene family is not olfactory mucosa but testis. This expression correlates with the presence of the corresponding protein during sperm cell maturation, and on mature sperm cells. The pattern of expression is consistent with a role as sensor for unidentified chemicals possibly involved in the control of mammalian sperm maturation, migration, and/or fertilization.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Steiger, S. S., Fidler, A. E., Kempenaers, B. (2008). Detection of Olfactory Receptor Transcripts in Bird Testes. J Hered 99: 624-628 [Abstract] [Full Text]
Suarez, S. S. (2008). Control of hyperactivation in sperm. Hum Reprod Update 14: 647-657 [Abstract] [Full Text]
Saraiva, L. R., Korsching, S. I. (2007). A novel olfactory receptor gene family in teleost fish. Genome Res 17: 1448-1457 [Abstract] [Full Text]
Xia, J., Reigada, D., Mitchell, C. H, Ren, D. (2007). CATSPER Channel-Mediated Ca2+ Entry into Mouse Sperm Triggers a Tail-to-Head Propagation. Biol. Reprod. 77: 551-559 [Abstract] [Full Text]
Naz, R. K., Sellamuthu, R. (2006). Receptors in Spermatozoa: Are They Real?. J Androl 27: 627-636 [Full Text]
Neuhaus, E. M., Mashukova, A., Barbour, J., Wolters, D., Hatt, H. (2006). Novel function of {beta}-arrestin2 in the nucleus of mature spermatozoa. J. Cell Sci. 119: 3047-3056 [Abstract] [Full Text]
Neuhaus, E. M., Mashukova, A., Zhang, W., Barbour, J., Hatt, H. (2006). A Specific Heat Shock Protein Enhances the Expression of Mammalian Olfactory Receptor Proteins. Chem Senses 31: 445-452 [Abstract] [Full Text]
Schuh, S. M., Carlson, A. E., McKnight, G. S., Conti, M., Hille, B., Babcock, D. F. (2006). Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists. Biol. Reprod. 74: 492-500 [Abstract] [Full Text]
Suarez, S.S., Pacey, A. A. (2006). Sperm transport in the female reproductive tract. Hum Reprod Update 12: 23-37 [Abstract] [Full Text]
Fukuda, N., Touhara, K. (2006). Developmental expression patterns of testicular olfactory receptor genes during mouse spermatogenesis. GENES CELLS 11: 71-81 [Abstract] [Full Text]
Fukuda, N., Yomogida, K., Okabe, M., Touhara, K. (2004). Functional characterization of a mouse testicular olfactory receptor and its role in chemosensing and in regulation of sperm motility. J. Cell Sci. 117: 5835-5845 [Abstract] [Full Text]
Spehr, M., Schwane, K., Riffell, J. A., Barbour, J., Zimmer, R. K., Neuhaus, E. M., Hatt, H. (2004). Particulate Adenylate Cyclase Plays a Key Role in Human Sperm Olfactory Receptor-mediated Chemotaxis. J. Biol. Chem. 279: 40194-40203 [Abstract] [Full Text]
Volz, A., Ehlers, A., Younger, R., Forbes, S., Trowsdale, J., Schnorr, D., Beck, S., Ziegler, A. (2003). Complex Transcription and Splicing of Odorant Receptor Genes. J. Biol. Chem. 278: 19691-19701 [Abstract] [Full Text]
Spehr, M., Gisselmann, G., Poplawski, A., Riffell, J. A., Wetzel, C. H., Zimmer, R. K., Hatt, H. (2003). Identification of a Testicular Odorant Receptor Mediating Human Sperm Chemotaxis. Science 299: 2054-2058 [Abstract] [Full Text]
Goto, T., Salpekar, A., Monk, M. (2001). Expression of a testis-specific member of the olfactory receptor gene family in human primordial germ cells. Mol Hum Reprod 7: 553-558 [Abstract] [Full Text]
Branscomb, A., Seger, J., White, R. L. (2000). Evolution of Odorant Receptors Expressed in Mammalian Testes. Genetics 156: 785-797 [Abstract] [Full Text]
Goto, T., Adjaye, J., Rodeck, C. H., Monk, M. (1999). Identification of genes expressed in human primordial germ cells at the time of entry of the female germ line into meiosis. Mol Hum Reprod 5: 851-860 [Abstract] [Full Text]
Syed, V., Gomez, E., Hecht, N. B. (1999). mRNAs Encoding a von Ebner's-like Protein and the Huntington Disease Protein Are Induced in Rat Male Germ Cells by Sertoli Cells. J. Biol. Chem. 274: 10737-10742 [Abstract] [Full Text]
Darszon, A., Labarca, P., Nishigaki, T., Espinosa, F. (1999). Ion Channels in Sperm Physiology. Physiol. Rev. 79: 481-510 [Abstract] [Full Text]
Gautier-Courteille, C., Salanova, M., Conti, M. (1998). The Olfactory Adenylyl Cyclase III Is Expressed in Rat Germ Cells during Spermiogenesis. Endocrinology 139: 2588-2599 [Abstract] [Full Text]
Walensky, L. D., Ruat, M., Bakin, R. E., Blackshaw, S., Ronnett, G. V., Snyder, S. H. (1998). Two Novel Odorant Receptor Families Expressed in Spermatids Undergo 5'-Splicing. J. Biol. Chem. 273: 9378-9387 [Abstract] [Full Text]
Saberan-Djoneidi, D., Picart, R., Escalier, D., Gelman, M., Barret, A., Tougard, C., Glowinski, J., Levi-Strauss, M. (1998). A 21-kDa Polypeptide Belonging to a New Family of Proteins Is Expressed in the Golgi Apparatus of Neural and Germ Cells. J. Biol. Chem. 273: 3909-3914 [Abstract] [Full Text]
Nef, S., Nef, P. (1997). Olfaction: Transient expression of a putative odorant receptor in the�avian�notochord. Proc. Natl. Acad. Sci. USA 94: 4766-4771 [Abstract] [Full Text]
Shepherd, G. M., Singer, M. S., Greer, C. A. (1996). {blacksquare} REVIEW : Olfactory Receptors: A Large Gene Family with Broad Affinities and Multiple Functions. Neuroscientist 2: 262-271 [Abstract]
Berruti, G, Borgonovo, B (1996). sp42, the boar sperm tyrosine kinase, is a male germ cell-specific product with a highly conserved tissue expression extending to other mammalian species. J. Cell Sci. 109: 851-858 [Abstract]
Bookbinder, L., Cheng, A, Bleil, J. (1995). Tissue- and species-specific expression of sp56, a mouse sperm fertilization protein. Science 269: 86-89 [Abstract]
Ryba, N. J. P., Tirindelli, R. (1995). A Novel GTP-binding Protein [IMAGE]-Subunit, G[IMAGE]8, Is Expressed during Neurogenesis in the Olfactory and Vomeronasal Neuroepithelia. J. Biol. Chem. 270: 6757-6767 [Abstract] [Full Text]