zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly

doi:10.1083/jcb.123.6.1727

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zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly

O Kuge, S Hara-Kuge, L Orci, M Ravazzola, M Amherdt, G Tanigawa, FT Wieland and JE Rothman
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.

cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon- COP), zeta-COP exists in both coatomer bound and free pools.
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