Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts

doi:10.1083/jcb.123.6.1789

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Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts

KS Ha and JH Exton
Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0295.

alpha-Thrombin induced a change in the cell morphology of IIC9 fibroblasts from a semiround to an elongated form, accompanied by an increase in stress fibers. Incubation of the cells with phospholipase D (PLD) from Streptomyces chromofuscus and exogenous phosphatidic acid (PA) caused similar morphological changes, whereas platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA) induced different changes, e.g., disruption of stress fibers and cell rounding. alpha-Thrombin, PDGF, and exogenous PLD increased PA by 20-40%, and PMA produced a smaller increase. alpha-Thrombin and exogenous PLD produced rapid increases in the amount of filamentous actin (F-actin) that were sustained for at least 60 min. However, PDGF produced a transient increase of F-actin at 1 min and PMA caused no significant change. Dioctanoylglycerol was ineffective except at 50 micrograms/ml. Phospholipase C from Bacillus cereus, which increased diacylglycerol (DAG) but not PA, did not change F-actin content. Down-regulation of protein kinase C (PKC) did not block actin polymerization induced by alpha-thrombin. H-7 was also ineffective. Exogenous PA activated actin polymerization with a significant effect at 0.01 microgram/ml and a maximal increase at 1 microgram/ml. No other phospholipids tested, including polyphosphoinositides, significantly activated actin polymerization. PDGF partially inhibited PA-induced actin polymerization after an initial increase at 1 min. PMA completely or largely blocked actin polymerization induced by PA or PLD. These results show that PC-derived PA, but not DAG or PKC, activates actin polymerization in IIC9 fibroblasts, and indicate that PDGF and PMA have inhibitory effects on PA-induced actin polymerization.
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Kusner, D. J., Barton, J. A., Wen, K.-K., Wang, X., Rubenstein, P. A., Iyer, S. S. (2002). Regulation of Phospholipase D Activity by Actin. ACTIN EXERTS BIDIRECTIONAL MODULATION OF MAMMALIAN PHOSPOLIPASE D ACTIVITY IN A POLYMERIZATION-DEPENDENT, ISOFORM-SPECIFIC MANNER. J. Biol. Chem. 277: 50683-50692 [Abstract] [Full Text]
Baker, C. A., Desrosiers, K., Dolan, J. W. (2002). Propranolol Inhibits Hyphal Development in Candida albicans. Antimicrob. Agents Chemother. 46: 3617-3620 [Abstract] [Full Text]
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Buchan, A. M. J., Lin, C.-Y., Choi, J., Barber, D. L. (2002). Somatostatin, Acting at Receptor Subtype 1, Inhibits Rho Activity, the Assembly of Actin Stress Fibers, and Cell Migration. J. Biol. Chem. 277: 28431-28438 [Abstract] [Full Text]
Hairfield, M. L., Westwater, C., Dolan, J. W. (2002). Phosphatidylinositol-4-phosphate 5-kinase activity is stimulated during temperature-induced morphogenesis in Candida albicans. Microbiology 148: 1737-1746 [Abstract] [Full Text]
Reynaert, H, Thompson, M G, Thomas, T, Geerts, A (2002). Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension. Gut 50: 571-581 [Abstract] [Full Text]
Nakajima, K.-i., Sonoda, H., Mizoguchi, T., Aoki, J., Arai, H., Nagahama, M., Tagaya, M., Tani, K. (2002). A Novel Phospholipase A1 with Sequence Homology to a Mammalian Sec23p-interacting Protein, p125. J. Biol. Chem. 277: 11329-11335 [Abstract] [Full Text]
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Gardiner, J. C., Harper, J. D. I., Weerakoon, N. D., Collings, D. A., Ritchie, S., Gilroy, S., Cyr, R. J., Marc, J. (2001). A 90-kD Phospholipase D from Tobacco Binds to Microtubules and the Plasma Membrane. Plant Cell 13: 2143-2158 [Abstract] [Full Text]
Kam, Y., Exton, J. H. (2001). Phospholipase D Activity Is Required for Actin Stress Fiber Formation in Fibroblasts. Mol. Cell. Biol. 21: 4055-4066 [Abstract] [Full Text]
Kusner, D. J., Adams, J. (2000). ATP-Induced Killing of Virulent Mycobacterium tuberculosis Within Human Macrophages Requires Phospholipase D. J. Immunol. 164: 379-388 [Abstract] [Full Text]
Kusner, D. J., Hall, C. F., Jackson, S. (1999). Fc{gamma} Receptor-Mediated Activation of Phospholipase D Regulates Macrophage Phagocytosis of IgG-Opsonized Particles. J. Immunol. 162: 2266-2274 [Abstract] [Full Text]
Iyer, S. S., Kusner, D. J. (1999). Association of Phospholipase D Activity with the Detergent-insoluble Cytoskeleton of U937 Promonocytic Leukocytes. J. Biol. Chem. 274: 2350-2359 [Abstract] [Full Text]
Rizzo, M. A., Shome, K., Vasudevan, C., Stolz, D. B., Sung, T.-C., Frohman, M. A., Watkins, S. C., Romero, G. (1999). Phospholipase D and Its Product, Phosphatidic Acid, Mediate Agonist-dependent Raf-1 Translocation to the Plasma Membrane and the Activation of the Mitogen-activated Protein Kinase Pathway. J. Biol. Chem. 274: 1131-1139 [Abstract] [Full Text]
Norman, J.C., Jones, D., Barry, S.T., Holt, M.R., Cockcroft, S., Critchley, D.R. (1998). ARF1 Mediates Paxillin Recruitment to Focal Adhesions and Potentiates Rho-stimulated Stress Fiber Formation in Intact and Permeabilized Swiss 3T3 Fibroblasts. JCB 143: 1981-1995 [Abstract] [Full Text]
Frank, S. R., Hatfield, J. C., Casanova, J. E. (1998). Remodeling of the Actin Cytoskeleton Is Coordinately Regulated by Protein Kinase C and the ADP-Ribosylation Factor Nucleotide Exchange Factor ARNO. Mol. Biol. Cell 9: 3133-3146 [Abstract] [Full Text]
Steed, P. M., Clark, K. L., Boyar, W. C., Lasala, D. J. (1998). Characterization of human PLD2 and the analysis of PLD isoform splice variants. FASEB J. 12: 1309-1317 [Abstract] [Full Text]
Brindley, D. N., Waggoner, D. W. (1998). Mammalian Lipid Phosphate Phosphohydrolases. J. Biol. Chem. 273: 24281-24284 [Full Text]
Hess, J. A., Ji, Q.-s., Carpenter, G., Exton, J. H. (1998). Analysis of Platelet-derived Growth Factor-induced Phospholipase D Activation in Mouse Embryo Fibroblasts Lacking Phospholipase C-gamma 1. J. Biol. Chem. 273: 20517-20524 [Abstract] [Full Text]
Lee, Z.-W., Kweon, S.-M., Kim, B.-C., Leem, S.-H., Shin, I., Kim, J.-H., Ha, K.-S. (1998). Phosphatidic Acid-induced Elevation of Intracellular Ca2+ Is Mediated by RhoA and H2O2 in Rat-2 Fibroblasts. J. Biol. Chem. 273: 12710-12715 [Abstract] [Full Text]
Higgs, H. N., Han, M. H., Johnson, G. E., Glomset, J. A. (1998). Cloning of a Phosphatidic Acid-preferring Phospholipase A1 from Bovine Testis. J. Biol. Chem. 273: 5468-5477 [Abstract] [Full Text]
Goh, E. L. K., Pircher, T. J., Wood, T. J. J., Norstedt, G., Graichen, R., Lobie, P. E. (1997). Growth Hormone-Induced Reorganization of the Actin Cytoskeleton Is Not Required for STAT5 (Signal Transducer and Activator of Transcription-5)-Mediated Transcription. Endocrinology 138: 3207-3215 [Abstract] [Full Text]
Exton, J. H. (1997). New Developments in Phospholipase D. J. Biol. Chem. 272: 15579-15582 [Full Text]
Waggoner, D. W., Gomez-Munoz, A., Dewald, J., Brindley, D. N. (1996). Phosphatidate Phosphohydrolase Catalyzes the Hydrolysis of Ceramide 1-Phosphate, Lysophosphatidate, and Sphingosine 1-Phosphate. J. Biol. Chem. 271: 16506-16509 [Abstract] [Full Text]
Munnik, T., Vrije, T. d., Irvine, R. F., Musgrave, A. (1996). Identification of Diacylglycerol Pyrophosphate as a Novel Metabolic Product of Phosphatidic Acid during G-protein Activation in Plants. J. Biol. Chem. 271: 15708-15715 [Abstract] [Full Text]
Flores, I., Casaseca, T., Martinez-A, C., Kanoh, H., Merida, I. (1996). Phosphatidic Acid Generation through Interleukin 2 (IL-2)-induced alpha-Diacylglycerol Kinase Activation Is an Essential Step in IL-2-mediated Lymphocyte Proliferation. J. Biol. Chem. 271: 10334-10340 [Abstract] [Full Text]
Knoepp, S. M., Wisehart-Johnson, A. E., Buse, M. G., Bradshaw, C. D., Ella, K. M., Meier, K. E. (1996). Synergistic Effects of Insulin and Phorbol Ester on Mitogen-activated Protein Kinase in Rat-1 HIR Cells. J. Biol. Chem. 271: 1678-1686 [Abstract] [Full Text]
Zhou, D., Luini, W., Bernasconi, S., Diomede, L., Salmona, M., Mantovani, A., Sozzani, S. (1995). Phosphatidic Acid and Lysophosphatidic Acid Induce Haptotactic Migration of Human Monocytes. J. Biol. Chem. 270: 25549-25556 [Abstract] [Full Text]
Waggoner, D. W., Martin, A., Dewald, J., G�mez-Mu�oz, A., Brindley, D. N. (1995). Purification and Characterization of a Novel Plasma Membrane Phosphatidate Phosphohydrolase from Rat Liver. J. Biol. Chem. 270: 19422-19429 [Abstract] [Full Text]
Horiguchi, Y, Senda, T, Sugimoto, N, Katahira, J, Matsuda, M (1995). Bordetella bronchiseptica dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesions by modifying the small GTP-binding protein rho. J. Cell Sci. 108: 3243-3251 [Abstract]
Park, J. B., Kim, J. H., Kim, Y., Ha, S. H., Kim, J. H., Yoo, J.-S., Du, G., Frohman, M. A., Suh, P.-G., Ryu, S. H. (2000). Cardiac Phospholipase D2 Localizes to Sarcolemmal Membranes and Is Inhibited by alpha -Actinin in an ADP-ribosylation Factor-reversible Manner. J. Biol. Chem. 275: 21295-21301 [Abstract] [Full Text]
Aragones, J., Jones, D. R., Martin, S., Juan, M. A. S., Alfranca, A., Vidal, F., Vara, A., Merida, I., Landazuri, M. O. (2001). Evidence for the Involvement of Diacylglycerol Kinase in the Activation of Hypoxia-inducible Transcription Factor 1 by Low Oxygen Tension. J. Biol. Chem. 276: 10548-10555 [Abstract] [Full Text]
Lee, S., Park, J. B., Kim, J. H., Kim, Y., Kim, J. H., Shin, K.-J., Lee, J. S., Ha, S. H., Suh, P.-G., Ryu, S. H. (2001). Actin Directly Interacts with Phospholipase D, Inhibiting Its Activity. J. Biol. Chem. 276: 28252-28260 [Abstract] [Full Text]
Powner, D. J., Hodgkin, M. N., Wakelam, M. J.O. (2002). Antigen-stimulated Activation of Phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 Cells. Mol. Biol. Cell 13: 1252-1262 [Abstract] [Full Text]