A small rab GTPase is distributed in cytoplasmic vesicles in non polarized cells but colocalizes with the tight junction marker ZO-1 in polarized epithelial cells

doi:10.1083/jcb.124.1.101

This Article

	Full Text (PDF, 6789K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Zahraoui, A.
	Articles by Louvard, D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Zahraoui, A.
	Articles by Louvard, D.


Social Bookmarking

	What's this?

ARTICLES

A small rab GTPase is distributed in cytoplasmic vesicles in non polarized cells but colocalizes with the tight junction marker ZO-1 in polarized epithelial cells

A Zahraoui, G Joberty, M Arpin, JJ Fontaine, R Hellio, A Tavitian and D Louvard
Institut National de la Sante et de la Recherche Medicale U. 248, Faculte de medecine Lariboisiere Saint Louis, Paris, France.

Small rab/Ypt1/Sec4 GTPase family have been involved in the regulation of membrane traffic along the biosynthetic and endocytic pathways in eucaryotic cells. Polarized epithelial cells have morphologically and functionally distinct apical and basolateral surfaces separated by tight junctions. The establishment and maintenance of these structures require delivery of membrane proteins and lipids to these domains. In this work, we have isolated a cDNA clone from a human intestinal cDNA library encoding a small GTPase, rab13, closely related to the yeast Sec4 protein. Confocal microscopy analysis on polarized Caco-2 cells shows that rab13 protein colocalized with the tight junction marker ZO- 1. Cryostat sections of tissues confirm that rab13 localized to the junctional complex region of a variety of epithelia, including intestine, kidney, liver, and of endothelial cells. This localization requires assembly and integrity of the tight junctions. Disruption of tight junctions by incubation in low Ca2+ media induces the redistribution of rab13. In cells devoid of tight junctions, rab13 was found associated with vesicles dispersed throughout the cytoplasm. Cell- cell contacts initiated by E-cadherin in transfected L cells do not recruit rab13 to the resulting adherens-like junction complexes. The participation of rab13 in polarized transport, in the assembly and/or the activity of tight junctions is discussed.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Mruk, D. D., Lau, A. S.N. (2009). RAB13 Participates in Ectoplasmic Specialization Dynamics in the Rat Testis. Biol. Reprod. 80: 590-601 [Abstract] [Full Text]
Beyenbach, K. W., Baumgart, S., Lau, K., Piermarini, P. M., Zhang, S. (2009). Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome of Aedes Malpighian tubules. J. Exp. Biol. 212: 329-340 [Abstract] [Full Text]
Fasano, A. (2008). Physiological, Pathological, and Therapeutic Implications of Zonulin-Mediated Intestinal Barrier Modulation: Living Life on the Edge of the Wall. Am. J. Pathol. 173: 1243-1252 [Abstract] [Full Text]
Shen, L., Weber, C. R., Turner, J. R. (2008). The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state. JCB 181: 683-695 [Abstract] [Full Text]
Nakatsuji, H., Nishimura, N., Yamamura, R., Kanayama, H.-o., Sasaki, T. (2008). Involvement of Actinin-4 in the Recruitment of JRAB/MICAL-L2 to Cell-Cell Junctions and the Formation of Functional Tight Junctions. Mol. Cell. Biol. 28: 3324-3335 [Abstract] [Full Text]
Yang, W., Qiu, C., Biswas, N., Jin, J., Watkins, S. C., Montelaro, R. C., Coyne, C. B., Wang, T. (2008). Correlation of the Tight Junction-like Distribution of Claudin-1 to the Cellular Tropism of Hepatitis C Virus. J. Biol. Chem. 283: 8643-8653 [Abstract] [Full Text]
Yamamura, R., Nishimura, N., Nakatsuji, H., Arase, S., Sasaki, T. (2008). The Interaction of JRAB/MICAL-L2 with Rab8 and Rab13 Coordinates the Assembly of Tight Junctions and Adherens Junctions. Mol. Biol. Cell 19: 971-983 [Abstract] [Full Text]
Babbey, C. M., Ahktar, N., Wang, E., Chen, C. C.-H., Grant, B. D., Dunn, K. W. (2006). Rab10 Regulates Membrane Transport through Early Endosomes of Polarized Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 17: 3156-3175 [Abstract] [Full Text]
Terai, T., Nishimura, N., Kanda, I., Yasui, N., Sasaki, T. (2006). JRAB/MICAL-L2 Is a Junctional Rab13-binding Protein Mediating the Endocytic Recycling of Occludin. Mol. Biol. Cell 17: 2465-2475 [Abstract] [Full Text]
Morimoto, S., Nishimura, N., Terai, T., Manabe, S., Yamamoto, Y., Shinahara, W., Miyake, H., Tashiro, S., Shimada, M., Sasaki, T. (2005). Rab13 Mediates the Continuous Endocytic Recycling of Occludin to the Cell Surface. J. Biol. Chem. 280: 2220-2228 [Abstract] [Full Text]
Mruk, D. D., Cheng, C. Y. (2004). Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis. Endocr. Rev. 25: 747-806 [Abstract] [Full Text]
Cereijido, M., Contreras, R. G., Shoshani, L. (2004). Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans. Physiol. Rev. 84: 1229-1262 [Abstract] [Full Text]
Powell, R. R., Temesvari, L. A. (2004). Involvement of a Rab8-like protein of Dictyostelium discoideum, Sas1, in the formation of membrane extensions, secretion and adhesion during development. Microbiology 150: 2513-2525 [Abstract] [Full Text]
Bazzoni, G., Dejana, E. (2004). Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis. Physiol. Rev. 84: 869-901 [Abstract] [Full Text]
Schneeberger, E. E., Lynch, R. D. (2004). The tight junction: a multifunctional complex. Am. J. Physiol. Cell Physiol. 286: C1213-C1228 [Abstract] [Full Text]
Kohler, K., Louvard, D., Zahraoui, A. (2004). Rab13 regulates PKA signaling during tight junction assembly. JCB 165: 175-180 [Abstract] [Full Text]
Yeaman, C., Grindstaff, K. K., Nelson, W. J. (2004). Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells. J. Cell Sci. 117: 559-570 [Abstract] [Full Text]
Guan, Y., Rubenstein, N. M., Failor, K. L., Woo, P. L., Firestone, G. L. (2004). Glucocorticoids Control {beta}-Catenin Protein Expression and Localization through Distinct Pathways that Can Be Uncoupled by Disruption of Signaling Events Required for Tight Junction Formation in Rat Mammary Epithelial Tumor Cells. Mol. Endocrinol. 18: 214-227 [Abstract] [Full Text]
TUMA, P. L., HUBBARD, A. L. (2003). Transcytosis: Crossing Cellular Barriers. Physiol. Rev. 83: 871-932 [Abstract] [Full Text]
Li, X., Low, S. H., Miura, M., Weimbs, T. (2002). SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes. Am. J. Physiol. Renal Physiol. 283: F1111-F1122 [Abstract] [Full Text]
Cheng, C. Y., Mruk, D. D. (2002). Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development. Physiol. Rev. 82: 825-874 [Abstract] [Full Text]
Marzesco, A.-M., Dunia, I., Pandjaitan, R., Recouvreur, M., Dauzonne, D., Benedetti, E. L., Louvard, D., Zahraoui, A. (2002). The Small GTPase Rab13 Regulates Assembly of Functional Tight Junctions in Epithelial Cells. Mol. Biol. Cell 13: 1819-1831 [Abstract] [Full Text]
Low, S. H., Marmorstein, L. Y., Miura, M., Li, X., Kudo, N., Marmorstein, A. D., Weimbs, T. (2002). Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype. J. Cell Sci. 115: 4545-4553 [Abstract] [Full Text]
Tiwari-Woodruff, S. K., Buznikov, A. G., Vu, T. Q., Micevych, P. E., Chen, K., Kornblum, H. I., Bronstein, J. M. (2001). Osp/Claudin-11 Forms a Complex with a Novel Member of the Tetraspanin Super Family and {beta}1 Integrin and Regulates Proliferation and Migration of Oligodendrocytes. JCB 153: 295-306 [Abstract] [Full Text]
Zahraoui, A., Louvard, D., Galli, T. (2000). Tight Junction, a Platform for Trafficking and Signaling Protein Complexes. JCB 151: 31-36 [Full Text]
Mitic, L. L., Van Itallie, C. M., Anderson, J. M. (2000). Molecular Physiology and Pathophysiology of Tight Junctions I. Tight junction structure and function: lessons from mutant animals and proteins. Am. J. Physiol. Gastrointest. Liver Physiol. 279: G250-G254 [Abstract] [Full Text]
Obert, G., Peiffer, I., Servin, A. L. (2000). Rotavirus-Induced Structural and Functional Alterations in Tight Junctions of Polarized Intestinal Caco-2 Cell Monolayers. J. Virol. 74: 4645-4651 [Abstract] [Full Text]
Clarke, H, Soler, A., Mullin, J. (2000). Protein kinase C activation leads to dephosphorylation of occludin and tight junction permeability increase in LLC-PK1 epithelial cell sheets. J. Cell Sci. 113: 3187-3196 [Abstract]
Sheth, B, Moran, B, Anderson, J., Fleming, T. (2000). Post-translational control of occludin membrane assembly in mouse trophectoderm: a mechanism to regulate timing of tight junction biogenesis and blastocyst formation. Development 127: 831-840 [Abstract]
Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. (1999). Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins. JCB 147: 1351-1363 [Abstract] [Full Text]
Woo, P. L., Ching, D., Guan, Y., Firestone, G. L. (1999). Requirement for Ras and Phosphatidylinositol 3-Kinase Signaling Uncouples the Glucocorticoid-induced Junctional Organization and Transepithelial Electrical Resistance in Mammary Tumor Cells. J. Biol. Chem. 274: 32818-32828 [Abstract] [Full Text]
Tsukamoto, T., Nigam, S. K. (1999). Role of tyrosine phosphorylation in the reassembly of occludin and other tight junction proteins. Am. J. Physiol. Renal Physiol. 276: F737-F750 [Abstract] [Full Text]
Schweiger, S., Foerster, J., Lehmann, T., Suckow, V., Muller, Y. A., Walter, G., Davies, T., Porter, H., van Bokhoven, H., Lunt, P. W., Traub, P., Ropers, H.-H. (1999). The Opitz syndrome gene product, MID1, associates with microtubules. Proc. Natl. Acad. Sci. USA 96: 2794-2799 [Abstract] [Full Text]
Wong, V., Ching, D., McCrea, P. D., Firestone, G. L. (1999). Glucocorticoid Down-regulation of Fascin Protein Expression Is Required for the Steroid-induced Formation of Tight Junctions and Cell-Cell Interactions in Rat Mammary Epithelial Tumor Cells. J. Biol. Chem. 274: 5443-5453 [Abstract] [Full Text]
Lapierre, L., Tuma, P., Navarre, J, Goldenring, J., Anderson, J. (1999). VAP-33 localizes to both an intracellular vesicle population and with occludin at the tight junction. J. Cell Sci. 112: 3723-3732 [Abstract]
YEAMAN, C., GRINDSTAFF, K. K., NELSON, W. J. (1999). New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79: 73-98 [Abstract] [Full Text]
Field, F. J., Born, E., Murthy, S., Mathur, S. N. (1998). Caveolin is present in intestinal cells: role in cholesterol trafficking?. J. Lipid Res. 39: 1938-1950 [Abstract] [Full Text]
Cho, M. M., Ziats, N. P., Abdul-Karim, F. W., Pal, D., Goldfarb, J., Utian, W. H., Gorodeski, G. I. (1998). Effects of Estrogen on Tight Junctional Resistance in Cultured Human Umbilical Vein Endothelial Cells. Reproductive Sciences 5: 260-270 [Abstract]
Marzesco, A.-M., Galli, T., Louvard, D., Zahraoui, A. (1998). The Rod cGMP Phosphodiesterase delta �Subunit Dissociates the Small GTPase Rab13 from Membranes. J. Biol. Chem. 273: 22340-22345 [Abstract] [Full Text]
Coconnier, M.-H., Dlissi, E., Robard, M., Laboisse, C. L., Gaillard, J.-L., Servin, A. L. (1998). Listeria monocytogenes Stimulates Mucus Exocytosis in Cultured Human Polarized Mucosecreting Intestinal Cells through Action of Listeriolysin O. Infect. Immun. 66: 3673-3681 [Abstract] [Full Text]
Balda, M., Matter, K (1998). Tight junctions. J. Cell Sci. 111: 541-547 [Abstract]
Matter, K, Balda, M. (1998). Biogenesis of tight junctions: the C-terminal domain of occludin mediates basolateral targeting. J. Cell Sci. 111: 511-519 [Abstract]
Denker, B. M., Nigam, S. K. (1998). Molecular structure and assembly of the tight junction. Am. J. Physiol. Renal Physiol. 274: F1-F9 [Abstract] [Full Text]
Takaishi, K., Sasaki, T., Kotani, H., Nishioka, H., Takai, Y. (1997). Regulation of Cell-Cell Adhesion by Rac and Rho Small G Proteins in MDCK Cells. JCB 139: 1047-1059 [Abstract] [Full Text]
Turner, J. R., Rill, B. K., Carlson, S. L., Carnes, D., Kerner, R., Mrsny, R. J., Madara, J. L. (1997). Physiological regulation of epithelial tight junctions is associated with myosin light-chain phosphorylation. Am. J. Physiol. Cell Physiol. 273: C1378-C1385 [Abstract] [Full Text]
Chen, Y.-h., Merzdorf, C., Paul, D. L., Goodenough, D. A. (1997). COOH Terminus of Occludin Is Required for Tight Junction Barrier Function in Early Xenopus Embryos. JCB 138: 891-899 [Abstract] [Full Text]
Wong, V., Gumbiner, B. M. (1997). A Synthetic Peptide Corresponding to the Extracellular Domain of Occludin Perturbs the Tight Junction Permeability Barrier. JCB 136: 399-409 [Abstract] [Full Text]
Cordenonsi, M, Mazzon, E, De Rigo, L, Baraldo, S, Meggio, F, Citi, S (1997). Occludin dephosphorylation in early development of Xenopus laevis. J. Cell Sci. 110: 3131-3139 [Abstract]
Sheth, B, Fesenko, I, Collins, J., Moran, B, Wild, A., Anderson, J., Fleming, T. (1997). Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform. Development 124: 2027-2037 [Abstract]
Merzdorf, C., Goodenough, D. (1997). Localization of a novel 210 kDa protein in Xenopus tight junctions. J. Cell Sci. 110: 1005-1012 [Abstract]
Decaens, C, Rodriguez, P, Bouchaud, C, Cassio, D (1996). Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9. A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one. J. Cell Sci. 109: 1623-1635 [Abstract]
Woo, P. L., Cha, H. H., Singer, K. L., Firestone, G. L. (1996). Antagonistic Regulation of Tight Junction Dynamics by Glucocorticoids and Transforming Growth Factor-beta in Mouse Mammary Epithelial Cells. J. Biol. Chem. 271: 404-412 [Abstract] [Full Text]
Citi, S, Denisenko, N (1995). Phosphorylation of the tight junction protein cingulin and the effects of protein kinase inhibitors and activators in MDCK epithelial cells. J. Cell Sci. 108: 2917-2926 [Abstract]
Yonemura, S, Itoh, M, Nagafuchi, A, Tsukita, S (1995). Cell-to-cell adherens junction formation and actin filament organization: similarities and differences between non-polarized fibroblasts and polarized epithelial cells. J. Cell Sci. 108: 127-142 [Abstract]
Deretic, D, Huber, L., Ransom, N, Mancini, M, Simons, K, Papermaster, D. (1995). rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis. J. Cell Sci. 108: 215-224 [Abstract]
Lutcke, A, Parton, R., Murphy, C, Olkkonen, V., Dupree, P, Valencia, A, Simons, K, Zerial, M (1994). Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression. J. Cell Sci. 107: 3437-3448 [Abstract]
Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N. M., Choudhary, J. S., Lewis, A. P., Chotai, D., Fazel, A., Gushue, J. N., Paiement, J., Palcy, S., Chevet, E., Lafreniere-Roula, M., Solari, R., Thomas, D. Y., Rowley, A., Bergeron, J. J. M. (2001). Proteomics Characterization of Abundant Golgi Membrane Proteins. J. Biol. Chem. 276: 5152-5165 [Abstract] [Full Text]
Bazzoni, G., Martinez-Estrada, O. M., Orsenigo, F., Cordenonsi, M., Citi, S., Dejana, E. (2000). Interaction of Junctional Adhesion Molecule with the Tight Junction Components ZO-1, Cingulin, and Occludin. J. Biol. Chem. 275: 20520-20526 [Abstract] [Full Text]