Expression of a dominant allele of human ARF1 inhibits membrane traffic in vivo

doi:10.1083/jcb.124.3.289

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Expression of a dominant allele of human ARF1 inhibits membrane traffic in vivo

CJ Zhang, AG Rosenwald, MC Willingham, S Skuntz, J Clark and RA Kahn
Laboratory of Biological Chemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

ADP-ribosylation factor (ARF) proteins and inhibitory peptides derived from ARFs have demonstrated activities in a number of in vitro assays that measure ER-to-Golgi and intra-Golgi transport and endosome fusion. To better understand the roles of ARF proteins in vivo, stable cell lines were obtained from normal rat kidney (NRK) cells transfected with either wild-type or a dominant activating allele ([Q71L]) of the human ARF1 gene under the control of the interferon-inducible mouse Mx1 promoter. Upon addition of interferon, expression of ARF1 proteins increased with a half-time of 7-8 h, as determined by immunoblot analysis. Induction of mutant ARF1, but not wild-type ARF1, led to an inhibition of protein secretion with kinetics similar to that observed for induction of protein expression. Examination of the Golgi apparatus and the ER by indirect immunofluorescence or transmission electron microscopy revealed that expression of low levels of mutant ARF1 protein correlated with a dramatic increase in vesiculation of the Golgi apparatus and expansion of the ER lumen, while expression of substantially higher levels of wild-type ARF1 had no discernible effect. Endocytosis was also inhibited by expression of mutant ARF1, but not by the wild-type protein. Finally, the expression of [Q71L]ARF1, but not wild-type ARF1, antagonized the actions of brefeldin A, as determined by the delayed loss of ARF and beta-COP from Golgi membranes and disruption of the Golgi apparatus. General models for the actions of ARF1 in membrane traffic events are discussed.
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Shin, H.-W., Kobayashi, H., Kitamura, M., Waguri, S., Suganuma, T., Uchiyama, Y., Nakayama, K. (2005). Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J. Cell Sci. 118: 4039-4048 [Abstract] [Full Text]
Haynes, L. P., Thomas, G. M. H., Burgoyne, R. D. (2005). Interaction of Neuronal Calcium Sensor-1 and ADP-ribosylation Factor 1 Allows Bidirectional Control of Phosphatidylinositol 4-Kinase {beta} and trans-Golgi Network-Plasma Membrane Traffic. J. Biol. Chem. 280: 6047-6054 [Abstract] [Full Text]
Xu, J., Scheres, B. (2005). Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarity. Plant Cell 17: 525-536 [Abstract] [Full Text]
Matsukawa, J., Nakayama, K., Nagao, T., Ichijo, H., Urushidani, T. (2003). Role of ADP-ribosylation Factor 6 (ARF6) in Gastric Acid Secretion. J. Biol. Chem. 278: 36470-36475 [Abstract] [Full Text]
Garcia-Mata, R., Szul, T., Alvarez, C., Sztul, E. (2003). ADP-Ribosylation Factor/COPI-dependent Events at the Endoplasmic Reticulum-Golgi Interface Are Regulated by the Guanine Nucleotide Exchange Factor GBF1. Mol. Biol. Cell 14: 2250-2261 [Abstract] [Full Text]
Feng, Y., Yu, S., Lasell, T. K. R., Jadhav, A. P., Macia, E., Chardin, P., Melancon, P., Roth, M., Mitchison, T., Kirchhausen, T. (2003). Exo1: A new chemical inhibitor of the exocytic pathway. Proc. Natl. Acad. Sci. USA 100: 6469-6474 [Abstract] [Full Text]
Santy, L. C. (2002). Characterization of a Fast Cycling ADP-ribosylation Factor 6 Mutant. J. Biol. Chem. 277: 40185-40188 [Abstract] [Full Text]
Boman, A. L., Salo, P. D., Hauglund, M. J., Strand, N. L., Rensink, S. J., Zhdankina, O. (2002). ADP-Ribosylation Factor (ARF) Interaction Is Not Sufficient for Yeast GGA Protein Function or Localization. Mol. Biol. Cell 13: 3078-3095 [Abstract] [Full Text]
Yu, S., Roth, M. G. (2002). Casein Kinase I Regulates Membrane Binding by ARF GAP1. Mol. Biol. Cell 13: 2559-2570 [Abstract] [Full Text]
Yoo, J.-S., Moyer, B. D., Bannykh, S., Yoo, H.-M., Riordan, J. R., Balch, W. E. (2002). Non-conventional Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator through the Early Secretory Pathway. J. Biol. Chem. 277: 11401-11409 [Abstract] [Full Text]
Stephens, D. J., Pepperkok, R. (2002). Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells. J. Cell Sci. 115: 1149-1160 [Abstract] [Full Text]
Lu, L., Horstmann, H., Ng, C., Hong, W. (2002). Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J. Cell Sci. 114: 4543-4555 [Abstract] [Full Text]
Shinotsuka, C., Yoshida, Y., Kawamoto, K., Takatsu, H., Nakayama, K. (2002). Overexpression of an ADP-ribosylation Factor-Guanine Nucleotide Exchange Factor, BIG2, Uncouples Brefeldin A-induced Adaptor Protein-1 Coat Dissociation and Membrane Tubulation. J. Biol. Chem. 277: 9468-9473 [Abstract] [Full Text]
Furman, C., Short, S. M., Subramanian, R. R., Zetter, B. R., Roberts, T. M. (2002). DEF-1/ASAP1 Is a GTPase-activating Protein (GAP) for ARF1 That Enhances Cell Motility through a GAP-dependent Mechanism. J. Biol. Chem. 277: 7962-7969 [Abstract] [Full Text]
de Figueiredo, P., Doody, A., Polizotto, R. S., Drecktrah, D., Wood, S., Banta, M., Strang, M. S., Brown, W. J. (2001). Inhibition of Transferrin Recycling and Endosome Tubulation by Phospholipase A2 Antagonists. J. Biol. Chem. 276: 47361-47370 [Abstract] [Full Text]
Brown, F. D., Rozelle, A. L., Yin, H. L., Balla, T., Donaldson, J. G. (2001). Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic. JCB 154: 1007-1018 [Abstract] [Full Text]
Mazaki, Y., Hashimoto, S., Okawa, K., Tsubouchi, A., Nakamura, K., Yagi, R., Yano, H., Kondo, A., Iwamatsu, A., Mizoguchi, A., Sabe, H. (2001). An ADP-Ribosylation Factor GTPase-activating Protein Git2-short/KIAA0148 Is Involved in Subcellular Localization of Paxillin and Actin Cytoskeletal Organization. Mol. Biol. Cell 12: 645-662 [Abstract] [Full Text]
Boman, A. L. (2001). GGA proteins: new players in the sorting game. J. Cell Sci. 114: 3413-3418 [Abstract] [Full Text]
Martin, M. E., Hidalgo, J., Rosa, J. L., Crottet, P., Velasco, A. (2000). Effect of Protein Kinase A Activity on the Association of ADP-ribosylation Factor 1 to Golgi Membranes. J. Biol. Chem. 275: 19050-19059 [Abstract] [Full Text]
Shome, K., Rizzo, M. A., Vasudevan, C., Andresen, B., Romero, G. (2000). The Activation of Phospholipase D by Endothelin-1, Angiotensin II, and Platelet-Derived Growth Factor in Vascular Smooth Muscle A10 Cells Is Mediated by Small G Proteins of the ADP-Ribosylation Factor Family. Endocrinology 141: 2200-2208 [Abstract] [Full Text]
Zhu, X., Boman, A. L., Kuai, J., Cieplak, W., Kahn, R. A. (2000). Effectors Increase the Affinity of ADP-ribosylation Factor for GTP to Increase Binding. J. Biol. Chem. 275: 13465-13475 [Abstract] [Full Text]
Boman, A. L., Zhang, C.-j., Zhu, X., Kahn, R. A. (2000). A Family of ADP-Ribosylation Factor Effectors That Can Alter Membrane Transport through the trans-Golgi. Mol. Biol. Cell 11: 1241-1255 [Abstract] [Full Text]
Kondo, A., Hashimoto, S., Yano, H., Nagayama, K., Mazaki, Y., Sabe, H. (2000). A New Paxillin-binding Protein, PAG3/Papalpha /KIAA0400, Bearing an ADP-Ribosylation Factor GTPase-activating Protein Activity, Is Involved in Paxillin Recruitment to Focal Adhesions and Cell Migration. Mol. Biol. Cell 11: 1315-1327 [Abstract] [Full Text]
Gu, F., Gruenberg, J. (2000). ARF1 Regulates pH-dependent COP Functions in the Early Endocytic Pathway. J. Biol. Chem. 275: 8154-8160 [Abstract] [Full Text]
Kuai, J., Boman, A. L., Arnold, R. S., Zhu, X., Kahn, R. A. (2000). Effects of Activated ADP-ribosylation Factors on Golgi Morphology Require neither Activation of Phospholipase D1 nor Recruitment of Coatomer. J. Biol. Chem. 275: 4022-4032 [Abstract] [Full Text]
Cuvillier, A, Redon, F, Antoine, J., Chardin, P, DeVos, T, Merlin, G (2000). LdARL-3A, a Leishmania promastigote-specific ADP-ribosylation factor-like protein, is essential for flagellum integrity. J. Cell Sci. 113: 2065-2074 [Abstract]
Pepperkok, R, Whitney, J., Gomez, M, Kreis, T. (2000). COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo. J. Cell Sci. 113: 135-144 [Abstract]
Sharer, J. D., Kahn, R. A. (1999). The ARF-like 2 (ARL2)-binding Protein, BART. PURIFICATION, CLONING, AND INITIAL CHARACTERIZATION. J. Biol. Chem. 274: 27553-27561 [Abstract] [Full Text]
Jones, S., Jedd, G., Kahn, R. A., Franzusoff, A., Bartolini, F., Segev, N. (1999). Genetic Interactions in Yeast Between Ypt GTPases and Arf Guanine Nucleotide Exchangers. Genetics 152: 1543-1556 [Abstract] [Full Text]
Huang, C.-F., Buu, L.-M., Yu, W.-L., Lee, F.-J. S. (1999). Characterization of a Novel ADP-ribosylation Factor-like Protein (yARL3) in Saccharomyces cerevisiae. J. Biol. Chem. 274: 3819-3827 [Abstract] [Full Text]
Premont, R. T., Claing, A., Vitale, N., Freeman, J. L.�R., Pitcher, J. A., Patton, W. A., Moss, J., Vaughan, M., Lefkowitz, R. J. (1998). beta 2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein. Proc. Natl. Acad. Sci. USA 95: 14082-14087 [Abstract] [Full Text]
Shome, K., Nie, Y., Romero, G. (1998). ADP-ribosylation Factor Proteins Mediate Agonist-induced Activation of Phospholipase D. J. Biol. Chem. 273: 30836-30841 [Abstract] [Full Text]
Nelson, D. S., Alvarez, C., Gao, Y.-s., Garcia-Mata, R., Fialkowski, E., Sztul, E. (1998). The Membrane Transport Factor TAP/p115 Cycles between the Golgi and Earlier Secretory Compartments and Contains Distinct Domains Required for Its Localization and Function. JCB 143: 319-331 [Abstract] [Full Text]
Franco, M., Boretto, J., Robineau, S., Monier, S., Goud, B., Chardin, P., Chavrier, P. (1998). ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1,�is involved in the control of Golgi structure and function. Proc. Natl. Acad. Sci. USA 95: 9926-9931 [Abstract] [Full Text]
Ooi, C. E., Dell'Angelica, E. C., Bonifacino, J. S. (1998). ADP-Ribosylation Factor 1 (ARF1) Regulates Recruitment of the AP-3 Adaptor Complex to Membranes. JCB 142: 391-402 [Abstract] [Full Text]
Luo, J.-Q., Liu, X., Frankel, P., Rotunda, T., Ramos, M., Flom, J., Jiang, H., Feig, L. A., Morris, A. J., Kahn, R. A., Foster, D. A. (1998). Functional association between Arf and RalA in active phospholipase D complex. Proc. Natl. Acad. Sci. USA 95: 3632-3637 [Abstract] [Full Text]
Monier, S, Chardin, P, Robineau, S, Goud, B (1998). Overexpression of the ARF1 exchange factor ARNO inhibits the early secretory pathway and causes the disassembly of the Golgi complex. J. Cell Sci. 111: 3427-3436 [Abstract]
Song, J, Khachikian, Z, Radhakrishna, H, Donaldson, J. (1998). Localization of endogenous ARF6 to sites of cortical actin rearrangement and involvement of ARF6 in cell spreading. J. Cell Sci. 111: 2257-2267 [Abstract]
Pepperkok, R, Lowe, M, Burke, B, Kreis, T. (1998). Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S. J. Cell Sci. 111: 1877-1888 [Abstract]
Vasudevan, C, Han, W, Tan, Y, Nie, Y, Li, D, Shome, K, Watkins, S., Levitan, E., Romero, G (1998). The distribution and translocation of the G protein ADP-ribosylation factor 1 in live cells is determined by its GTPase activity. J. Cell Sci. 111: 1277-1285 [Abstract]
Yang, W., Storrie, B. (1998). Scattered Golgi Elements during Microtubule Disruption Are Initially Enriched in Trans-Golgi Proteins. Mol. Biol. Cell 9: 191-207 [Abstract] [Full Text]
Daro, E., Sheff, D., Gomez, M., Kreis, T., Mellman, I. (1997). Inhibition of Endosome Function in CHO Cells Bearing a Temperature-sensitive Defect in the Coatomer (COPI) Component {varepsilon}-COP. JCB 139: 1747-1759 [Abstract] [Full Text]
Lee, F.-J. S., Huang, C.-F., Yu, W.-L., Buu, L.-M., Lin, C.-Y., Huang, M.-C., Moss, J., Vaughan, M. (1997). Characterization of an ADP-ribosylation Factor-like 1�Protein in Saccharomyces cerevisiae. J. Biol. Chem. 272: 30998-31005 [Abstract] [Full Text]
Jareb, M., Banker, G. (1997). Inhibition of Axonal Growth by Brefeldin A in Hippocampal Neurons in Culture. J. Neurosci. 17: 8955-8963 [Abstract] [Full Text]
Radhakrishna, H., Donaldson, J. G. (1997). ADP-Ribosylation Factor 6 Regulates a Novel Plasma Membrane Recycling Pathway. JCB 139: 49-61 [Abstract] [Full Text]
Kanoh, H., Williger, B.-T., Exton, J. H. (1997). Arfaptin 1,a Putative Cytosolic Target Protein of ADP-ribosylation Factor, Is Recruited to Golgi Membranes. J. Biol. Chem. 272: 5421-5429 [Abstract] [Full Text]
Gaynor, E. C., Emr, S. D. (1997). COPI-independent Anterograde Transport: Cargo-selective ER to Golgi Protein Transport in Yeast COPI Mutants. JCB 136: 789-802 [Abstract] [Full Text]
Erickson, J. W., Zhang, C.-j., Kahn, R. A., Evans, T., Cerione, R. A. (1996). Mammalian Cdc42 Is a Brefeldin A-sensitive Component of the Golgi Apparatus. J. Biol. Chem. 271: 26850-26854 [Abstract] [Full Text]
Cavenagh, M. M., Whitney, J. A., Carroll, K., Zhang, C.-j., Boman, A. L., Rosenwald, A. G., Mellman, I., Kahn, R. A. (1996). Intracellular Distribution of Arf Proteins in Mammalian Cells. Arf6 IS UNIQUELY LOCALIZED TO THE PLASMA MEMBRANE. J. Biol. Chem. 271: 21767-21774 [Abstract] [Full Text]
Chen, Y.-G., Shields, D. (1996). ADP-ribosylation Factor-1 Stimulates Formation of Nascent Secretory Vesicles from the trans-Golgi Network of Endocrine Cells. J. Biol. Chem. 271: 5297-5300 [Abstract] [Full Text]
Le Borgne, R., Griffiths, G., Hoflack, B. (1996). Mannose 6-Phosphate Receptors and ADP-ribosylation Factors Cooperate for High Affinity Interaction of the AP-1 Golgi Assembly Proteins with Membranes. J. Biol. Chem. 271: 2162-2170 [Abstract] [Full Text]
Lowe, S., Wong, S., Hong, W (1996). The mammalian ARF-like protein 1 (Arl1) is associated with the Golgi complex. J. Cell Sci. 109: 209-220 [Abstract]
Spiro, D. J., Taylor, T. C., Melanon, P., Wessling-Resnick, M. (1995). Cytosolic ADP-ribosylation Factors Are Not Required for Endosome-Endosome Fusion but Are Necessary for GTP[IMAGE]S Inhibition of Fusion. J. Biol. Chem. 270: 13693-13697 [Abstract] [Full Text]
Moss, J., Vaughan, M. (1995). Structure and Function of ARF Proteins: Activators of Cholera Toxin and Critical Components of Intracellular Vesicular Transport Processes. J. Biol. Chem. 270: 12327-12330 [Full Text]
Bourgoin, S., Harbour, D., Desmarais, Y., Takai, Y., Beaulieu, A. (1995). Low Molecular Weight GTP-binding Proteins in HL-60 Granulocytes. J. Biol. Chem. 270: 3172-3178 [Abstract] [Full Text]
Kahn, R. A., Clark, J., Rulka, C., Stearns, T., Zhang, C.-j., Randazzo, P. A., Terui, T., Cavenagh, M. (1995). Mutational Analysis of Saccharomyces cerevisiae ARF1. J. Biol. Chem. 270: 143-150 [Abstract] [Full Text]
Donaldson, J.G., Radhakrishna, H., Peters, P.J. (1995). The ARF GTPases: Defining Roles in Membrane Traffic and Organelle Structure. Cold Spring Harb Symp Quant Biol 60: 229-234 [Abstract]
Liendo, A., Stedman, T. T., Ngo, H. M., Chaturvedi, S., Hoppe, H. C., Joiner, K. A. (2001). Toxoplasma gondii ADP-ribosylation Factor 1 Mediates Enhanced Release of Constitutively Secreted Dense Granule Proteins. J. Biol. Chem. 276: 18272-18281 [Abstract] [Full Text]
Zhu, X., Kahn, R. A. (2001). The Escherichia coli Heat Labile Toxin Binds to Golgi Membranes and Alters Golgi and Cell Morphologies Using ADP-ribosylation Factor-dependent Processes. J. Biol. Chem. 276: 25014-25021 [Abstract] [Full Text]
Morinaga, N., Kaihou, Y., Vitale, N., Moss, J., Noda, M. (2001). Involvement of ADP-ribosylation Factor 1 in Cholera Toxin-induced Morphological Changes of Chinese Hamster Ovary Cells. J. Biol. Chem. 276: 22838-22843 [Abstract] [Full Text]
Van Valkenburgh, H., Shern, J. F., Sharer, J. D., Zhu, X., Kahn, R. A. (2001). ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) Have Both Specific and Shared Effectors. CHARACTERIZING ARL1-BINDING PROTEINS. J. Biol. Chem. 276: 22826-22837 [Abstract] [Full Text]
Sharer, J. D., Shern, J. F., Van Valkenburgh, H., Wallace, D. C., Kahn, R. A. (2002). ARL2 and BART Enter Mitochondria and Bind the Adenine Nucleotide Transporter. Mol. Biol. Cell 13: 71-83 [Abstract] [Full Text]