KIF3A is a new microtubule-based anterograde motor in the nerve axon

doi:10.1083/jcb.125.5.1095

This Article

	Full Text (PDF, 3662K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kondo, S.
	Articles by Hirokawa, N.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kondo, S.
	Articles by Hirokawa, N.


Social Bookmarking

	What's this?

ARTICLES

KIF3A is a new microtubule-based anterograde motor in the nerve axon

S Kondo, R Sato-Yoshitake, Y Noda, H Aizawa, T Nakata, Y Matsuura and N Hirokawa
Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan.

Neurons are highly polarized cells composed of dendrites, cell bodies, and long axons. Because of the lack of protein synthesis machinery in axons, materials required in axons and synapses have to be transported down the axons after synthesis in the cell body. Fast anterograde transport conveys different kinds of membranous organelles such as mitochondria and precursors of synaptic vesicles and axonal membranes, while organelles such as endosomes and autophagic prelysosomal organelles are conveyed retrogradely. Although kinesin and dynein have been identified as good candidates for microtubule-based anterograde and retrograde transporters, respectively, the existence of other motors for performing these complex axonal transports seems quite likely. Here we characterized a new member of the kinesin super-family, KIF3A (50-nm rod with globular head and tail), and found that it is localized in neurons, associated with membrane organelle fractions, and accumulates with anterogradely moving membrane organelles after ligation of peripheral nerves. Furthermore, native KIF3A (a complex of 80/85 KIF3A heavy chain and a 95-kD polypeptide) revealed microtubule gliding activity and baculovirus-expressed KIF3A heavy chain demonstrated microtubule plus end-directed (anterograde) motility in vitro. These findings strongly suggest that KIF3A is a new motor protein for the anterograde fast axonal transport.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Choi, J., Ha, C. M., Choi, E. J., Jeong, C. S., Park, J. W., Baik, J.-H., Park, J.-Y., Costa, M. E., Ojeda, S. R., Lee, B. J. (2008). Kinesin Superfamily-Associated Protein 3 Is Preferentially Expressed in Glutamatergic Neurons and Contributes to the Excitatory Control of Female Puberty. Endocrinology 149: 6146-6156 [Abstract] [Full Text]
Hirokawa, N., Noda, Y. (2008). Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics. Physiol. Rev. 88: 1089-1118 [Abstract] [Full Text]
Park, J. J., Cawley, N. X., Loh, Y. P. (2008). Carboxypeptidase E Cytoplasmic Tail-Driven Vesicle Transport Is Key for Activity-Dependent Secretion of Peptide Hormones. Mol. Endocrinol. 22: 989-1005 [Abstract] [Full Text]
Marshall, W. F. (2008). The cell biological basis of ciliary disease. JCB 180: 17-21 [Abstract] [Full Text]
Chizhikov, V. V., Davenport, J., Zhang, Q., Shih, E. K., Cabello, O. A., Fuchs, J. L., Yoder, B. K., Millen, K. J. (2007). Cilia Proteins Control Cerebellar Morphogenesis by Promoting Expansion of the Granule Progenitor Pool. J. Neurosci. 27: 9780-9789 [Abstract] [Full Text]
Pan, X., Ou, G., Civelekoglu-Scholey, G., Blacque, O. E., Endres, N. F., Tao, L., Mogilner, A., Leroux, M. R., Vale, R. D., Scholey, J. M. (2006). Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. JCB 174: 1035-1045 [Abstract] [Full Text]
Haraguchi, K., Hayashi, T., Jimbo, T., Yamamoto, T., Akiyama, T. (2006). Role of the Kinesin-2 Family Protein, KIF3, during Mitosis. J. Biol. Chem. 281: 4094-4099 [Abstract] [Full Text]
Miller, R. M., Callahan, L. M., Casaceli, C., Chen, L., Kiser, G. L., Chui, B., Kaysser-Kranich, T. M., Sendera, T. J., Palaniappan, C., Federoff, H. J. (2004). Dysregulation of Gene Expression in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Lesioned Mouse Substantia Nigra. J. Neurosci. 24: 7445-7454 [Abstract] [Full Text]
Deacon, S. W., Serpinskaya, A. S., Vaughan, P. S., Fanarraga, M. L., Vernos, I., Vaughan, K. T., Gelfand, V. I. (2003). Dynactin is required for bidirectional organelle transport. JCB 160: 297-301 [Abstract] [Full Text]
Lee, J.-R., Shin, H., Ko, J., Choi, J., Lee, H., Kim, E. (2003). Characterization of the Movement of the Kinesin Motor KIF1A in Living Cultured Neurons. J. Biol. Chem. 278: 2624-2629 [Abstract] [Full Text]
Diefenbach, R. J., Miranda-Saksena, M., Diefenbach, E., Holland, D. J., Boadle, R. A., Armati, P. J., Cunningham, A. L. (2002). Herpes Simplex Virus Tegument Protein US11 Interacts with Conventional Kinesin Heavy Chain. J. Virol. 76: 3282-3291 [Abstract] [Full Text]
Aronov, S., Aranda, G., Behar, L., Ginzburg, I. (2002). Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules. J. Cell Sci. 115: 3817-3827 [Abstract] [Full Text]
Liu, Q., Zhou, J., Daiger, S. P., Farber, D. B., Heckenlively, J. R., Smith, J. E., Sullivan, L. S., Zuo, J., Milam, A. H., Pierce, E. A. (2002). Identification and Subcellular Localization of the RP1 Protein in Human and Mouse Photoreceptors. IOVS 43: 22-32 [Abstract] [Full Text]
Yang, Z., Roberts, E. A., Goldstein, L. S. B. (2001). Functional Analysis of Mouse Kinesin Motor Kif3C. Mol. Cell. Biol. 21: 5306-5311 [Abstract] [Full Text]
Goldstein, L. S. B. (2001). Kinesin molecular motors: Transport pathways, receptors, and human disease. Proc. Natl. Acad. Sci. USA 98: 6999-7003 [Abstract] [Full Text]
Miki, H., Setou, M., Kaneshiro, K., Hirokawa, N. (2001). All kinesin superfamily protein, KIF, genes in mouse and human. Proc. Natl. Acad. Sci. USA 98: 7004-7011 [Abstract] [Full Text]
Ginkel, L. M., Wordeman, L. (2000). Expression and Partial Characterization of Kinesin-related Proteins in Differentiating and Adult Skeletal Muscle. Mol. Biol. Cell 11: 4143-4158 [Abstract] [Full Text]
Shah, J. V., Flanagan, L. A., Janmey, P. A., Leterrier, J.-F. (2000). Bidirectional Translocation of Neurofilaments along Microtubules Mediated in Part by Dynein/Dynactin. Mol. Biol. Cell 11: 3495-3508 [Abstract] [Full Text]
Kanai, Y., Okada, Y., Tanaka, Y., Harada, A., Terada, S., Hirokawa, N. (2000). KIF5C, a Novel Neuronal Kinesin Enriched in Motor Neurons. J. Neurosci. 20: 6374-6384 [Abstract] [Full Text]
Takeda, S., Yamazaki, H., Seog, D.-H., Kanai, Y., Terada, S., Hirokawa, N. (2000). Kinesin Superfamily Protein 3 (Kif3) Motor Transports Fodrin-Associating Vesicles Important for Neurite Building. JCB 148: 1255-1266 [Abstract] [Full Text]
Ray, K., Perez, S. E., Yang, Z., Xu, J., Ritchings, B. W., Steller, H., Goldstein, L. S.B. (1999). Kinesin-II Is Required for Axonal Transport of Choline Acetyltransferase in Drosophila. JCB 147: 507-518 [Abstract] [Full Text]
Cole, D. G. (1999). Kinesin-Ii, Coming and Going. JCB 147: 463-466 [Full Text]
Brown, J. M., Marsala, C., Kosoy, R., Gaertig, J. (1999). Kinesin-II Is Preferentially Targeted to Assembling Cilia and Is Required for Ciliogenesis and Normal Cytokinesis in Tetrahymena. Mol. Biol. Cell 10: 3081-3096 [Abstract] [Full Text]
Takeda, S., Yonekawa, Y., Tanaka, Y., Okada, Y., Nonaka, S., Hirokawa, N. (1999). Left-Right Asymmetry and Kinesin Superfamily Protein KIF3A: New Insights in Determination of Laterality and Mesoderm Induction by kif3A-/- Mice Analysis. JCB 145: 825-836 [Abstract] [Full Text]
Marszalek, J. R., Weiner, J. A., Farlow, S. J., Chun, J., Goldstein, L. S.B. (1999). Novel Dendritic Kinesin Sorting Identified by Different Process Targeting of Two Related Kinesins: KIF21A and KIF21B. JCB 145: 469-479 [Abstract] [Full Text]
Marszalek, J. R., Ruiz-Lozano, P., Roberts, E., Chien, K. R., Goldstein, L. S.�B. (1999). Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc. Natl. Acad. Sci. USA 96: 5043-5048 [Abstract] [Full Text]
Miller, M. G., Mulholland, D. J., Vogl, A. W. (1999). Rat Testis Motor Proteins Associated with Spermatid Translocation (Dynein) and Spermatid Flagella (Kinesin-II). Biol. Reprod. 60: 1047-1056 [Abstract] [Full Text]
Porter, M. E., Bower, R., Knott, J. A., Byrd, P., Dentler, W. (1999). Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas. Mol. Biol. Cell 10: 693-712 [Abstract] [Full Text]
Muresan, V., Lyass, A., Schnapp, B. J. (1999). The Kinesin Motor KIF3A Is a Component of the Presynaptic Ribbon in Vertebrate Photoreceptors. J. Neurosci. 19: 1027-1037 [Abstract] [Full Text]
Signor, D., Wedaman, K. P., Rose, L. S., Scholey, J. M. (1999). Two Heteromeric Kinesin Complexes in Chemosensory Neurons and Sensory Cilia of Caenorhabditis elegans. Mol. Biol. Cell 10: 345-360 [Abstract] [Full Text]
Le Bot, N., Antony, C., White, J., Karsenti, E., Vernos, I. (1998). Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus. JCB 143: 1559-1573 [Abstract] [Full Text]
Tuma, M. C., Zill, A., Le Bot, N., Vernos, I., Gelfand, V. (1998). Heterotrimeric Kinesin II Is the Microtubule Motor Protein Responsible for Pigment Dispersion in Xenopus Melanophores. JCB 143: 1547-1558 [Abstract] [Full Text]
Rahman, A., Friedman, D. S., Goldstein, L. S.�B. (1998). Two Kinesin Light Chain Genes in Mice. IDENTIFICATION AND CHARACTERIZATION OF THE ENCODED PROTEINS. J. Biol. Chem. 273: 15395-15403 [Abstract] [Full Text]
Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., Rosenbaum, J. L. (1998). Chlamydomonas Kinesin-II-dependent Intraflagellar Transport (IFT): IFT Particles Contain Proteins Required for Ciliary Assembly in Caenorhabditis elegans Sensory Neurons. JCB 141: 993-1008 [Abstract] [Full Text]
Yonekawa, Y., Harada, A., Okada, Y., Funakoshi, T., Kanai, Y., Takei, Y., Terada, S., Noda, T., Hirokawa, N. (1998). Defect in Synaptic Vesicle Precursor Transport and Neuronal Cell Death in KIF1A Motor Protein-deficient Mice. JCB 141: 431-441 [Abstract] [Full Text]
Muresan, V., Abramson, T., Lyass, A., Winter, D., Porro, E., Hong, F., Chamberlin, N. L., Schnapp, B. J. (1998). KIF3C and KIF3A Form a Novel Neuronal Heteromeric Kinesin That Associates with Membrane Vesicles. Mol. Biol. Cell 9: 637-652 [Abstract] [Full Text]
Yang, Z., Goldstein, L. S.�B. (1998). Characterization of the KIF3C Neural Kinesin-like Motor from Mouse. Mol. Biol. Cell 9: 249-261 [Abstract] [Full Text]
Hirokawa, N. (1998). Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport. Science 279: 519-526 [Abstract] [Full Text]
Morris, R. L., Scholey, J. M. (1997). Heterotrimeric Kinesin-II Is Required for the Assembly of Motile 9+2 Ciliary Axonemes on Sea Urchin Embryos. JCB 138: 1009-1022 [Abstract] [Full Text]
Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., Kanai, Y., Hirokawa, N. (1997). Identification and classification of 16�new kinesin superfamily (KIF) proteins in mouse�genome. Proc. Natl. Acad. Sci. USA 94: 9654-9659 [Abstract] [Full Text]
Morfini, G., Quiroga, S., Rosa, A., Kosik, K., Caceres, A. (1997). Suppression of KIF2 in PC12 Cells Alters the Distribution of a Growth Cone Nonsynaptic Membrane Receptor and Inhibits Neurite Extension. JCB 138: 657-669 [Abstract] [Full Text]
Vashishtha, M., Walther, Z., Hall, J. L. (1996). The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. J. Cell Sci. 109: 541-549 [Abstract]
Beech, P., Pagh-Roehl, K, Noda, Y, Hirokawa, N, Burnside, B, Rosenbaum, J. (1996). Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors. J. Cell Sci. 109: 889-897 [Abstract]
Abe, K., Aoki, M., Kawagoe, J., Yoshida, T., Hattori, A., Kogure, K., Itoyama, Y. (1995). Ischemic Delayed Neuronal Death : A Mitochondrial Hypothesis. Stroke 26: 1478-1489 [Abstract] [Full Text]
Tanaka, Y, Zhang, Z, Hirokawa, N (1995). Identification and molecular evolution of new dynein-like protein sequences in rat brain. J. Cell Sci. 108: 1883-1893 [Abstract]
Ong, L.-L., Lim, A. P. C., Er, C. P. N., Kuznetsov, S. A., Yu, H. (2000). Kinectin-Kinesin Binding Domains and Their Effects on Organelle Motility. J. Biol. Chem. 275: 32854-32860 [Abstract] [Full Text]