Purification and characterization of smooth muscle cell caveolae

doi:10.1083/jcb.126.1.127

This Article

	Full Text (PDF, 3131K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Chang, W. J.
	Articles by Anderson, R. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Chang, W. J.
	Articles by Anderson, R. G.


Social Bookmarking

	What's this?

ARTICLES

Purification and characterization of smooth muscle cell caveolae

WJ Chang, YS Ying, KG Rothberg, NM Hooper, AJ Turner, HA Gambliel, J De Gunzburg, SM Mumby, AG Gilman and RG Anderson
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center at Dallas 75235.

Plasmalemmal caveolae are a membrane specialization that mediates transcytosis across endothelial cells and the uptake of small molecules and ions by both epithelial and connective tissue cells. Recent findings suggest that caveolae may, in addition, be involved in signal transduction. To better understand the molecular composition of this membrane specialization, we have developed a biochemical method for purifying caveolae from chicken smooth muscle cells. Biochemical and morphological markers indicate that we can obtain approximately 1.5 mg of protein in the caveolae fraction from approximately 100 g of chicken gizzard. Gel electrophoresis shows that there are more than 30 proteins enriched in caveolae relative to the plasma membrane. Among these proteins are: caveolin, a structural molecule of the caveolae coat; multiple, glycosylphosphatidylinositol-anchored membrane proteins; both G alpha and G beta subunits of heterotrimeric GTP-binding protein; and the Ras-related GTP-binding protein, Rap1A/B. The method we have developed will facilitate future studies on the structure and function of caveolae.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Zheng, Y. Z., Foster, L. J. (2009). Contributions of quantitative proteomics to understanding membrane microdomains. J. Lipid Res. 50: 1976-1985 [Abstract] [Full Text]
Chrzanowska-Wodnicka, M., Kraus, A. E., Gale, D., White, G. C. II, VanSluys, J. (2008). Defective angiogenesis, endothelial migration, proliferation, and MAPK signaling in Rap1b-deficient mice. Blood 111: 2647-2656 [Abstract] [Full Text]
Yuyama, K., Sekino-Suzuki, N., Sanai, Y., Kasahara, K. (2007). Translocation of Activated Heterotrimeric G Protein G{alpha}o to Ganglioside-enriched Detergent-resistant Membrane Rafts in Developing Cerebellum. J. Biol. Chem. 282: 26392-26400 [Abstract] [Full Text]
Sitrin, R. G., Emery, S. L., Sassanella, T. M., Blackwood, R. A., Petty, H. R. (2006). Selective Localization of Recognition Complexes for Leukotriene B4 and Formyl-Met-Leu-Phe within Lipid Raft Microdomains of Human Polymorphonuclear Neutrophils. J. Immunol. 177: 8177-8184 [Abstract] [Full Text]
Lin, W.-W., Lin, Y.-C., Chang, T.-Y., Tsai, S.-H., Ho, H.-C., Chen, Y.-T., Yang, V. C. (2006). Caveolin-1 Expression Is Associated with Plaque Formation in Hypercholesterolemic Rabbits. J. Histochem. Cytochem. 54: 897-904 [Abstract] [Full Text]
Schwencke, C., Braun-Dullaeus, R. C., Wunderlich, C., Strasser, R. H. (2006). Caveolae and caveolin in transmembrane signaling: Implications for human disease. Cardiovasc Res 70: 42-49 [Abstract] [Full Text]
Zulli, A., Buxton, B. F., Black, M. J., Ming, Z., Cameron, A., Hare, D. L. (2006). The Immunoquantification of Caveolin-1 and eNOS in Human and Rabbit Diseased Blood Vessels. J. Histochem. Cytochem. 54: 151-159 [Abstract] [Full Text]
El-Yazbi, A. F., Cho, W. J., Boddy, G., Schulz, R., Daniel, E. E. (2006). Impact of caveolin-1 knockout on NANC relaxation in circular muscles of the mouse small intestine compared with longitudinal muscles. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G394-G403 [Abstract] [Full Text]
Gilad, L. A., Bresler, T., Gnainsky, J., Smirnoff, P., Schwartz, B. (2005). Regulation of vitamin D receptor expression via estrogen-induced activation of the ERK 1/2 signaling pathway in colon and breast cancer cells. J Endocrinol 185: 577-592 [Abstract] [Full Text]
Schutzer, W. E., Reed, J. F., Mader, S. L. (2005). Decline in caveolin-1 expression and scaffolding of G protein receptor kinase-2 with age in Fischer 344 aortic vascular smooth muscle. Am. J. Physiol. Heart Circ. Physiol. 288: H2457-H2464 [Abstract] [Full Text]
Mandal, S. K., Iakhiaev, A., Pendurthi, U. R., Rao, L. V. M. (2005). Acute cholesterol depletion impairs functional expression of tissue factor in fibroblasts: modulation of tissue factor activity by membrane cholesterol. Blood 105: 153-160 [Abstract] [Full Text]
Shajahan, A. N., Tiruppathi, C., Smrcka, A. V., Malik, A. B., Minshall, R. D. (2004). G{beta}{gamma} Activation of Src Induces Caveolae-mediated Endocytosis in Endothelial Cells. J. Biol. Chem. 279: 48055-48062 [Abstract] [Full Text]
Zhang, H., Links, P. H., Ngsee, J. K., Tran, K., Cui, Z., Ko, K. W. S., Yao, Z. (2004). Localization of Low Density Lipoprotein Receptor-related Protein 1 to Caveolae in 3T3-L1 Adipocytes in Response to Insulin Treatment. J. Biol. Chem. 279: 2221-2230 [Abstract] [Full Text]
Baron, C. B., Coburn, R. F. (2004). Smooth muscle raft-like membranes. J. Lipid Res. 45: 41-53 [Abstract] [Full Text]
Je, H.-D., Gallant, C., Leavis, P. C., Morgan, K. G. (2004). Caveolin-1 regulates contractility in differentiated vascular smooth muscle. Am. J. Physiol. Heart Circ. Physiol. 286: H91-H98 [Abstract] [Full Text]
Sotgia, F., Woodman, S. E., Bonuccelli, G., Capozza, F., Minetti, C., Scherer, P. E., Lisanti, M. P. (2003). Phenotypic behavior of caveolin-3 R26Q, a mutant associated with hyperCKemia, distal myopathy, and rippling muscle disease. Am. J. Physiol. Cell Physiol. 285: C1150-C1160 [Abstract] [Full Text]
Gros, R., Afroze, T., You, X.-M., Kabir, G., Van Wert, R., Kalair, W., Hoque, A. E., Mungrue, I. N., Husain, M. (2003). Plasma Membrane Calcium ATPase Overexpression in Arterial Smooth Muscle Increases Vasomotor Responsiveness and Blood Pressure. Circ. Res. 93: 614-621 [Abstract] [Full Text]
Hendrickson, H., Chatterjee, S., Cao, S., Ruiz, M. M., Sessa, W. C., Shah, V. (2003). Influence of caveolin on constitutively activated recombinant eNOS: insights into eNOS dysfunction in BDL rat liver. Am. J. Physiol. Gastrointest. Liver Physiol. 285: G652-G660 [Abstract] [Full Text]
Pike, L. J. (2003). Lipid rafts: bringing order to chaos. J. Lipid Res. 44: 655-667 [Abstract] [Full Text]
Nanjundan, M., Possmayer, F. (2003). Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase. Am. J. Physiol. Lung Cell. Mol. Physiol. 284: L1-L23 [Abstract] [Full Text]
Leclerc, P. C., Auger-Messier, M., Lanctot, P. M., Escher, E., Leduc, R., Guillemette, G. (2002). A Polyaromatic Caveolin-Binding-Like Motif in the Cytoplasmic Tail of the Type 1 Receptor for Angiotensin II Plays an Important Role in Receptor Trafficking and Signaling. Endocrinology 143: 4702-4710 [Abstract] [Full Text]
Isshiki, M., Ying, Y.-s., Fujita, T., Anderson, R. G. W. (2002). A Molecular Sensor Detects Signal Transduction from Caveolae in Living Cells. J. Biol. Chem. 277: 43389-43398 [Abstract] [Full Text]
Nebl, T., Pestonjamasp, K. N., Leszyk, J. D., Crowley, J. L., Oh, S. W., Luna, E. J. (2002). Proteomic Analysis of a Detergent-resistant Membrane Skeleton from Neutrophil Plasma Membranes. J. Biol. Chem. 277: 43399-43409 [Abstract] [Full Text]
Razani, B., Woodman, S. E., Lisanti, M. P. (2002). Caveolae: From Cell Biology to Animal Physiology. Pharmacol. Rev. 54: 431-467 [Abstract] [Full Text]
Razani, B., Lisanti, M. P. (2001). Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein kinase A. Am. J. Physiol. Cell Physiol. 281: C1241-C1250 [Abstract] [Full Text]
Gkantiragas, I., Brugger, B., Stuven, E., Kaloyanova, D., Li, X.-Y., Lohr, K., Lottspeich, F., Wieland, F. T., Helms, J. B. (2001). Sphingomyelin-enriched Microdomains at the Golgi Complex. Mol. Biol. Cell 12: 1819-1833 [Abstract] [Full Text]
Ginés, S., Ciruela, F., Burgueño, J., Casadó, V., Canela, Enric. I., Mallol, J., Lluís, C., Franco, R. (2001). Involvement of Caveolin in Ligand-Induced Recruitment and Internalization of A1 Adenosine Receptor and Adenosine Deaminase in an Epithelial Cell Line. Mol. Pharmacol. 59: 1314-1323 [Abstract] [Full Text]
Feng, D., Flaumenhaft, R., Bandeira–Melo, C., Weller, P. F., Dvorak, A. M. (2001). Ultrastructural Localization of Vesicle-associated Membrane Protein(s) to Specialized Membrane Structures in Human Pericytes, Vascular Smooth Muscle Cells, Endothelial Cells, Neutrophils, and Eosinophils. J. Histochem. Cytochem. 49: 293-304 [Abstract] [Full Text]
Oh, P., Schnitzer, J. E. (2001). Segregation of Heterotrimeric G Proteins in Cell Surface Microdomains. Gq Binds Caveolin to Concentrate in Caveolae, whereas Gi and Gs Target Lipid Rafts by Default. Mol. Biol. Cell 12: 685-698 [Abstract] [Full Text]
Sunada, Y., Ohi, H., Hase, A., Ohi, H., Hosono, T., Arata, S., Higuchi, S., Matsumura, K., Shimizu, T. (2001). Transgenic mice expressing mutant caveolin-3 show severe myopathy associated with increased nNOS activity. Hum Mol Genet 10: 173-178 [Abstract] [Full Text]
Li, W., Liu, P, Pilcher, B., Anderson, R. (2001). Cell-specific targeting of caveolin-1 to caveolae, secretory vesicles, cytoplasm or mitochondria. J. Cell Sci. 114: 1397-1408 [Abstract]
Darby, P. J., Kwan, C. Y., Daniel, E. E. (2000). Caveolae from canine airway smooth muscle contain the necessary components for a role in Ca2+ handling. Am. J. Physiol. Lung Cell. Mol. Physiol. 279: L1226-L1235 [Abstract] [Full Text]
Lee, H., Volonte’, D., Galbiati, F., Iyengar, P., Lublin, D. M., Bregman, D. B., Wilson, M. T., Campos-Gonzalez Boumediene Bouzahzah, R., Pestell, R. G., Scherer, P. E., Lisanti, M. P. (2000). Constitutive and Growth Factor-Regulated Phosphorylation of Caveolin-1 Occurs at the Same Site (Tyr-14) in Vivo: Identification of a c-Src/Cav-1/Grb7 Signaling Cassette. Mol. Endocrinol. 14: 1750-1775 [Abstract] [Full Text]
Doyle, D. D., Goings, G., Upshaw-Earley, J., Ambler, S. K., Mondul, A., Palfrey, H. C., Page, E. (2000). Dystrophin Associates With Caveolae of Rat Cardiac Myocytes : Relationship to Dystroglycan. Circ. Res. 87: 480-488 [Abstract] [Full Text]
Wilson, B. S., Pfeiffer, J. R., Oliver, J. M. (2000). Observing Fc{varepsilon}ri Signaling from the Inside of the Mast Cell Membrane. JCB 149: 1131-1142 [Abstract] [Full Text]
Moffett, S., Brown, D. A., Linder, M. E. (2000). Lipid-dependent Targeting of G Proteins into Rafts. J. Biol. Chem. 275: 2191-2198 [Abstract] [Full Text]
Miotti, S, Bagnoli, M, Tomassetti, A, Colnaghi, M., Canevari, S (2000). Interaction of folate receptor with signaling molecules lyn and G(alpha)(i-3) in detergent-resistant complexes from the ovary carcinoma cell line IGROV1. J. Cell Sci. 113: 349-357 [Abstract]
Stahlhut, M., van Deurs, B. (2000). Identification of Filamin as a Novel Ligand for Caveolin-1: Evidence for the Organization of Caveolin-1-associated Membrane Domains by the Actin Cytoskeleton. Mol. Biol. Cell 11: 325-337 [Abstract] [Full Text]
Wang, Y., Windh, R. T., Chen, C. A., Manning, D. R. (1999). N-Myristoylation and beta gamma Play Roles beyond Anchorage in the Palmitoylation of the G Protein alpha o Subunit. J. Biol. Chem. 274: 37435-37442 [Abstract] [Full Text]
Babiychuk, E. B., Palstra, R.-J. T. S., Schaller, J., Kampfer, U., Draeger, A. (1999). Annexin VI Participates in the Formation of a Reversible, Membrane-Cytoskeleton Complex in Smooth Muscle Cells. J. Biol. Chem. 274: 35191-35195 [Abstract] [Full Text]
Davy, A., Gale, N. W., Murray, E. W., Klinghoffer, R. A., Soriano, P., Feuerstein, C., Robbins, S. M. (1999). Compartmentalized signaling by GPI-anchored ephrin-A5 requires the Fyn tyrosine kinase to regulate cellular adhesion. Genes Dev. 13: 3125-3135 [Abstract] [Full Text]
Razani, B., Rubin, C. S., Lisanti, M. P. (1999). Regulation of cAMP-mediated Signal Transduction via Interaction of Caveolins with the Catalytic Subunit of Protein Kinase A. J. Biol. Chem. 274: 26353-26360 [Abstract] [Full Text]
Galbiati, F., Volonte, D., Minetti, C., Chu, J. B., Lisanti, M. P. (1999). Phenotypic Behavior of Caveolin-3 Mutations That Cause Autosomal Dominant Limb Girdle Muscular Dystrophy (LGMD-1C). RETENTION OF LGMD-1C CAVEOLIN-3 MUTANTS WITHIN THE GOLGI COMPLEX. J. Biol. Chem. 274: 25632-25641 [Abstract] [Full Text]
Oh, P., Schnitzer, J. E. (1999). Immunoisolation of Caveolae with High Affinity Antibody Binding to the Oligomeric Caveolin Cage. TOWARD UNDERSTANDING THE BASIS OF PURIFICATION. J. Biol. Chem. 274: 23144-23154 [Abstract] [Full Text]
Schlegel, A., Schwab, R. B., Scherer, P. E., Lisanti, M. P. (1999). A Role for the Caveolin Scaffolding Domain in Mediating the Membrane Attachment of Caveolin-1. THE CAVEOLIN SCAFFOLDING DOMAIN IS BOTH NECESSARY AND SUFFICIENT FOR MEMBRANE BINDING IN VITRO. J. Biol. Chem. 274: 22660-22667 [Abstract] [Full Text]
Harris, D. A. (1999). Cellular Biology of Prion Diseases. Clin. Microbiol. Rev. 12: 429-444 [Abstract] [Full Text]
Schwencke, C., Yamamoto, M., Okumura, S., Toya, Y., Kim, S.-J., Ishikawa, Y. (1999). Compartmentation of Cyclic Adenosine 3',5'-Monophosphate Signaling in Caveolae. Mol. Endocrinol. 13: 1061-1070 [Abstract] [Full Text]
Stan, R.-V., Ghitescu, L., Jacobson, B. S., Palade, G. E. (1999). Isolation, Cloning, and Localization of Rat PV-1, a Novel Endothelial Caveolar Protein. JCB 145: 1189-1198 [Abstract] [Full Text]
Volonte, D., Galbiati, F., Li, S., Nishiyama, K., Okamoto, T., Lisanti, M. P. (1999). Flotillins/Cavatellins Are Differentially Expressed in Cells and Tissues and Form a Hetero-oligomeric Complex with Caveolins in Vivo. CHARACTERIZATION AND EPITOPE-MAPPING OF A NOVEL FLOTILLIN-1 MONOCLONAL ANTIBODY PROBE. J. Biol. Chem. 274: 12702-12709 [Abstract] [Full Text]
Bradbury, N. A., Clark, J. A., Watkins, S. C., Widnell, C. C., Smith, H. S. IV, Bridges, R. J. (1999). Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L659-L668 [Abstract] [Full Text]
Galbiati, F., Volonte, D., Meani, D., Milligan, G., Lublin, D. M., Lisanti, M. P., Parenti, M. (1999). The Dually Acylated NH2-terminal Domain of Gi1alpha Is Sufficient to Target a Green Fluorescent Protein Reporter to Caveolin-enriched Plasma Membrane Domains. PALMITOYLATION OF CAVEOLIN-1 IS REQUIRED FOR THE RECOGNITION OF DUALLY ACYLATED G-PROTEIN alpha �SUBUNITS IN VIVO. J. Biol. Chem. 274: 5843-5850 [Abstract] [Full Text]
Melkonian, K. A., Ostermeyer, A. G., Chen, J. Z., Roth, M. G., Brown, D. A. (1999). Role of Lipid Modifications in Targeting Proteins to Detergent-resistant Membrane Rafts. MANY RAFT PROTEINS ARE ACYLATED, WHILE FEW ARE PRENYLATED. J. Biol. Chem. 274: 3910-3917 [Abstract] [Full Text]
Viola, A., Schroeder, S., Sakakibara, Y., Lanzavecchia, A. (1999). T Lymphocyte Costimulation Mediated by Reorganization of Membrane Microdomains. Science 283: 680-682 [Abstract] [Full Text]
Huang, C., Duncan, J. A., Gilman, A. G., Mumby, S. M. (1999). Persistent membrane association of activated and depalmitoylated G protein alpha �subunits. Proc. Natl. Acad. Sci. USA 96: 412-417 [Abstract] [Full Text]
Gilbert, A, Paccaud, J., Foti, M, Porcheron, G, Balz, J, Carpentier, J. (1999). Direct demonstration of the endocytic function of caveolae by a cell-free assay. J. Cell Sci. 112: 1101-1110 [Abstract]
Shaul, P. W., Anderson, R. G.�W. (1998). Role of plasmalemmal caveolae in signal transduction. Am. J. Physiol. Lung Cell. Mol. Physiol. 275: L843-L851 [Abstract] [Full Text]
Arni, S., Keilbaugh, S. A., Ostermeyer, A. G., Brown, D. A. (1998). Association of GAP-43 with Detergent-resistant Membranes Requires Two Palmitoylated Cysteine Residues. J. Biol. Chem. 273: 28478-28485 [Abstract] [Full Text]
Yamamoto, M., Toya, Y., Schwencke, C., Lisanti, M. P., Myers Jr., M. G., Ishikawa, Y. (1998). Caveolin Is an Activator of Insulin Receptor Signaling. J. Biol. Chem. 273: 26962-26968 [Abstract] [Full Text]
Field, F. J., Born, E., Murthy, S., Mathur, S. N. (1998). Caveolin is present in intestinal cells: role in cholesterol trafficking?. J. Lipid Res. 39: 1938-1950 [Abstract] [Full Text]
Drmota, T., Novotny, J., Kim, G.-D., Eidne, K. A., Milligan, G., Svoboda, P. (1998). Agonist-induced Internalization of the G Protein G11alpha and Thyrotropin-releasing Hormone Receptors Proceed on Different Time Scales. J. Biol. Chem. 273: 21699-21707 [Abstract] [Full Text]
Furuchi, T., Anderson, R. G.�W. (1998). Cholesterol Depletion of Caveolae Causes Hyperactivation of Extracellular Signal-related Kinase (ERK). J. Biol. Chem. 273: 21099-21104 [Abstract] [Full Text]
Waugh, M. G., Lawson, D., Tan, S. K., Hsuan, J. J. (1998). Phosphatidylinositol 4-Phosphate Synthesis in Immunoisolated Caveolae-like Vesicles and Low Buoyant Density Non-caveolar Membranes. J. Biol. Chem. 273: 17115-17121 [Abstract] [Full Text]
Benlimame, N., Le, P. U., Nabi, I. R. (1998). Localization of Autocrine Motility Factor Receptor to Caveolae and Clathrin-independent Internalization of Its Ligand to Smooth Endoplasmic Reticulum. Mol. Biol. Cell 9: 1773-1786 [Abstract] [Full Text]
Isshiki, M., Ando, J., Korenaga, R., Kogo, H., Fujimoto, T., Fujita, T., Kamiya, A. (1998). Endothelial Ca2+ waves preferentially originate at specific loci in caveolin-rich cell edges. Proc. Natl. Acad. Sci. USA 95: 5009-5014 [Abstract] [Full Text]
Ishii, I., Saito, E., Izumi, T., Ui, M., Shimizu, T. (1998). Agonist-induced Sequestration, Recycling, and Resensitization of Platelet-activating Factor Receptor. ROLE OF CYTOPLASMIC TAIL PHOSPHORYLATION IN EACH PROCESS. J. Biol. Chem. 273: 9878-9885 [Abstract] [Full Text]
Toya, Y., Schwencke, C., Couet, J., Lisanti, M. P., Ishikawa, Y. (1998). Inhibition of Adenylyl Cyclase by Caveolin Peptides. Endocrinology 139: 2025-2031 [Abstract] [Full Text]
Uittenbogaard, A., Ying, Y.-s., Smart, E. J. (1998). Characterization of a Cytosolic Heat-shock Protein-Caveolin Chaperone Complex. INVOLVEMENT IN CHOLESTEROL TRAFFICKING. J. Biol. Chem. 273: 6525-6532 [Abstract] [Full Text]
Okamoto, T., Schlegel, A., Scherer, P. E., Lisanti, M. P. (1998). Caveolins, a Family of Scaffolding Proteins for Organizing "Preassembled Signaling Complexes" at the Plasma Membrane. J. Biol. Chem. 273: 5419-5422 [Full Text]
Breton, S., Lisanti, M. P., Tyszkowski, R., McLaughlin, M., Brown, D. (1998). Basolateral Distribution of Caveolin-1 in the Kidney: Absence from H+-ATPase-coated Endocytic Vesicles in Intercalated Cells. J. Histochem. Cytochem. 46: 205-214 [Abstract] [Full Text]
Schroeder, R. J., Ahmed, S. N., Zhu, Y., London, E., Brown, D. A. (1998). Cholesterol and Sphingolipid Enhance the Triton X-100 Insolubility of Glycosylphosphatidylinositol-anchored Proteins by Promoting the Formation of Detergent-insoluble Ordered Membrane Domains. J. Biol. Chem. 273: 1150-1157 [Abstract] [Full Text]
Deans, J. P., Robbins, S. M., Polyak, M. J., Savage, J. A. (1998). Rapid Redistribution of CD20 to a Low Density Detergent-insoluble Membrane Compartment. J. Biol. Chem. 273: 344-348 [Abstract] [Full Text]
Cameron, P. L., Ruffin, J. W., Bollag, R., Rasmussen, H., Cameron, R. S. (1997). Identification of Caveolin and Caveolin-Related Proteins in the Brain. J. Neurosci. 17: 9520-9535 [Abstract] [Full Text]
Liu, P., Ying, Y.-s., Anderson, R. G.�W. (1997). Platelet-derived growth factor activates mitogen-activated protein kinase in�isolated�caveolae. Proc. Natl. Acad. Sci. USA 94: 13666-13670 [Abstract] [Full Text]
Oka, N., Asai, K., Kudej, R. K., Edwards, J. G., Toya, Y., Schwencke, C., Vatner, D. E., Vatner, S. F., Ishikawa, Y. (1997). Downregulation of caveolin by chronic beta -adrenergic receptor stimulation in mice. Am. J. Physiol. Cell Physiol. 273: C1957-C1962 [Abstract] [Full Text]
Huang, C., Hepler, J. R., Chen, L. T., Gilman, A. G., Anderson, R. G.W., Mumby, S. M. (1997). Organization of G Proteins and Adenylyl Cyclase at the Plasma Membrane. Mol. Biol. Cell 8: 2365-2378 [Abstract] [Full Text]
Kasahara, K., Watanabe, Y., Yamamoto, T., Sanai, Y. (1997). Association of Src Family Tyrosine Kinase Lyn with Ganglioside GD3 in Rat Brain. POSSIBLE REGULATION OF Lyn BY GLYCOSPHINGOLIPID IN CAVEOLAE-LIKE DOMAINS. J. Biol. Chem. 272: 29947-29953 [Abstract] [Full Text]
Lupu, C., Goodwin, C. A., Westmuckett, A. D., Emeis, J. J., Scully, M. F., Kakkar, V. V., Lupu, F. (1997). Tissue Factor Pathway Inhibitor in Endothelial Cells Colocalizes With Glycolipid Microdomains/Caveolae : Regulatory Mechanism(s) of the Anticoagulant Properties of the Endothelium. Arterioscler. Thromb. Vasc. Bio. 17: 2964-2974 [Abstract] [Full Text]
Mastick, C. C., Saltiel, A. R. (1997). Insulin-stimulated Tyrosine Phosphorylation of Caveolin Is Specific for the Differentiated Adipocyte Phenotype in 3T3-L1 Cells. J. Biol. Chem. 272: 20706-20714 [Abstract] [Full Text]
Doyle, D. D., Ambler, S. K., Upshaw-Earley, J., Bastawrous, A., Goings, G. E., Page, E. (1997). Type B Atrial Natriuretic Peptide Receptor in Cardiac Myocyte Caveolae. Circ. Res. 81: 86-91 [Abstract] [Full Text]
de Weerd, W. F.�C., Leeb-Lundberg, L. M.�F. (1997). Bradykinin Sequesters B2 Bradykinin Receptors and the Receptor-coupled Galpha Subunits Galpha q and Galpha i in Caveolae in DDT1 MF-2 Smooth Muscle Cells. J. Biol. Chem. 272: 17858-17866 [Abstract] [Full Text]
Engelman, J. A., Wykoff, C. C., Yasuhara, S., Song, K. S., Okamoto, T., Lisanti, M. P. (1997). Recombinant Expression of Caveolin-1 in Oncogenically Transformed Cells Abrogates Anchorage-independent Growth. J. Biol. Chem. 272: 16374-16381 [Abstract] [Full Text]
Das, A. K., Dasgupta, B., Bhattacharya, R., Basu, J. (1997). Purification and Biochemical Characterization of a Protein-palmitoyl Acyltransferase from Human Erythrocytes. J. Biol. Chem. 272: 11021-11025 [Abstract] [Full Text]
Rehm, A., Ploegh, H. L. (1997). Assembly and Intracellular Targeting of the {beta}{gamma} Subunits of Heterotrimeric G Proteins. JCB 137: 305-317 [Abstract] [Full Text]
Bilderback, T. R., Grigsby, R. J., Dobrowsky, R. T. (1997). Association of p75NTR with Caveolin and Localization of Neurotrophin-induced Sphingomyelin Hydrolysis to Caveolae. J. Biol. Chem. 272: 10922-10927 [Abstract] [Full Text]
Liu, J., Oh, P., Horner, T., Rogers, R. A., Schnitzer, J. E. (1997). Organized Endothelial Cell Surface Signal Transduction in Caveolae Distinct from Glycosylphosphatidylinositol-anchored Protein Microdomains. J. Biol. Chem. 272: 7211-7222 [Abstract] [Full Text]
Couet, J., Li, S., Okamoto, T., Ikezu, T., Lisanti, M. P. (1997). Identification of Peptide and Protein Ligands for the Caveolin-scaffolding Domain. IMPLICATIONS FOR THE INTERACTION OF CAVEOLIN WITH CAVEOLAE-ASSOCIATED PROTEINS. J. Biol. Chem. 272: 6525-6533 [Abstract] [Full Text]
Song, K. S., Tang, Z., Li, S., Lisanti, M. P. (1997). Mutational Analysis of the Properties of Caveolin-1. A NOVEL ROLE FOR THE C-TERMINAL DOMAIN IN MEDIATING HOMO-TYPIC CAVEOLIN-CAVEOLIN INTERACTIONS. J. Biol. Chem. 272: 4398-4403 [Abstract] [Full Text]
Wu, C., Butz, S., Ying, Y.-s., Anderson, R. G.W. (1997). Tyrosine Kinase Receptors Concentrated in Caveolae-like Domains from Neuronal Plasma Membrane. J. Biol. Chem. 272: 3554-3559 [Abstract] [Full Text]
Kubler, E., Dohlman, H. G., Lisanti, M. P. (1996). Identification of Triton X-100 Insoluble Membrane Domains in the Yeast Saccharomyces cerevisiae. LIPID REQUIREMENTS FOR TARGETING OF HETEROTRIMERIC G-PROTEIN SUBUNITS. J. Biol. Chem. 271: 32975-32980 [Abstract] [Full Text]
Li, S., Couet, J., Lisanti, M. P. (1996). Src Tyrosine Kinases, Galpha Subunits, and H-Ras Share a Common Membrane-anchored Scaffolding Protein, Caveolin. CAVEOLIN BINDING NEGATIVELY REGULATES THE AUTO-ACTIVATION OF Src TYROSINE KINASES. J. Biol. Chem. 271: 29182-29190 [Abstract] [Full Text]
Smart, E. J., Ying, Y.-s., Donzell, W. C., Anderson, R. G.W. (1996). A Role for Caveolin in Transport of Cholesterol from Endoplasmic Reticulum to Plasma Membrane. J. Biol. Chem. 271: 29427-29435 [Abstract] [Full Text]
Li, S., Song, K. S., Koh, S. S., Kikuchi, A., Lisanti, M. P. (1996). Baculovirus-based Expression of Mammalian Caveolin in Sf21 Insect Cells. A MODEL SYSTEM FOR THE BIOCHEMICAL AND MORPHOLOGICAL STUDY OF CAVEOLAE BIOGENESIS. J. Biol. Chem. 271: 28647-28654 [Abstract] [Full Text]
Pike, L. J., Casey, L. (1996). Localization and Turnover of Phosphatidylinositol 4,5-Bisphosphate in Caveolin-enriched Membrane Domains. J. Biol. Chem. 271: 26453-26456 [Abstract] [Full Text]
Tolbert, L. M., Lameh, J. (1996). Human Muscarinic Cholinergic Receptor Hm1 Internalizes via Clathrin-coated Vesicles. J. Biol. Chem. 271: 17335-17342 [Abstract] [Full Text]
Song, K. S., Scherer, P. E., Tang, Z., Okamoto, T., Li, S., Chafel, M., Chu, C., Kohtz, D. S., Lisanti, M. P. (1996). Expression of Caveolin-3 in Skeletal, Cardiac, and Smooth Muscle Cells. CAVEOLIN-3 IS A COMPONENT OF THE SARCOLEMMA AND CO-FRACTIONATES WITH DYSTROPHIN AND DYSTROPHIN-ASSOCIATED GLYCOPROTEINS. J. Biol. Chem. 271: 15160-15165 [Abstract] [Full Text]
Mineo, C., James, G. L., Smart, E. J., Anderson, R. G. W. (1996). Localization of Epidermal Growth Factor-stimulated Ras/Raf-1 Interaction to Caveolae Membrane. J. Biol. Chem. 271: 11930-11935 [Abstract] [Full Text]
Liu, P., Ying, Y., Ko, Y.-G., Anderson, R. G. W. (1996). Localization of Platelet-derived Growth Factor-stimulated Phosphorylation Cascade to Caveolae. J. Biol. Chem. 271: 10299-10303 [Abstract] [Full Text]
Song, K. S., Li, S., Okamoto, T., Quilliam, L. A., Sargiacomo, M., Lisanti, M. P. (1996). Co-purification and Direct Interaction of Ras with Caveolin, an Integral Membrane Protein of Caveolae Microdomains. J. Biol. Chem. 271: 9690-9697 [Abstract] [Full Text]
Mu�oz, P.�n, Mora, S., Sevilla, L., Kaliman, P., Tom�s, E., Gum�, A., Testar, X., Palac�n, M., Zorzano, A. (1996). Expression and Insulin-regulated Distribution of Caveolin in Skeletal Muscle. J. Biol. Chem. 271: 8133-8139 [Abstract] [Full Text]
Bouillot, C., Prochiantz, A., Rougon, G.�v., Allinquant, B. (1996). Axonal Amyloid Precursor Protein Expressed by Neurons in Vitro Is Present in a Membrane Fraction with Caveolae-like Properties. J. Biol. Chem. 271: 7640-7644 [Abstract] [Full Text]