MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria

doi:10.1083/jcb.126.6.1375

This Article

	Full Text (PDF, 4065K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Burgess, S. M.
	Articles by Jensen, R. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Burgess, S. M.
	Articles by Jensen, R. E.


Social Bookmarking

	What's this?

ARTICLES

MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria

SM Burgess, M Delannoy and RE Jensen
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

In the yeast Saccharomyces cerevisiae, mitochondria are elongated organelles which form a reticulum around the cell periphery. To determine the mechanism by which mitochondrial shape is established and maintained, we screened yeast mutants for those defective in mitochondrial morphology. One of these mutants, mmm1, is temperature- sensitive for the external shape of its mitochondria. At the restrictive temperature, elongated mitochondria appear to quickly collapse into large, spherical organelles. Upon return to the permissive temperature, wild-type mitochondrial structure is restored. The morphology of other cellular organelles is not affected in mmm1 mutants, and mmm1 does not disrupt normal actin or tubulin organization. Cells disrupted in the MMM1 gene are inviable when grown on nonfermentable carbon sources and show abnormal mitochondrial morphology at all temperatures. The lethality of mmm1 mutants appears to result from the inability to segregate the aberrant-shaped mitochondria into daughter cells. Mitochondrial structure is therefore important for normal cell function. Mmm1p is located in the mitochondrial outer membrane, with a large carboxyl-terminal domain facing the cytosol. We propose that Mmm1p maintains mitochondria in an elongated shape by attaching the mitochondrion to an external framework, such as the cytoskeleton.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Liesa, M., Palacin, M., Zorzano, A. (2009). Mitochondrial Dynamics in Mammalian Health and Disease. Physiol. Rev. 89: 799-845 [Abstract] [Full Text]
Tamura, Y., Endo, T., Iijima, M., Sesaki, H. (2009). Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. JCB 185: 1029-1045 [Abstract] [Full Text]
Zhou, C., Huang, Y., Shao, Y., May, J., Prou, D., Perier, C., Dauer, W., Schon, E. A., Przedborski, S. (2008). The kinase domain of mitochondrial PINK1 faces the cytoplasm. Proc. Natl. Acad. Sci. USA 105: 12022-12027 [Abstract] [Full Text]
Jeong, J., Cohu, C., Kerkeb, L., Pilon, M., Connolly, E. L., Guerinot, M. L. (2008). Chloroplast Fe(III) chelate reductase activity is essential for seedling viability under iron limiting conditions. Proc. Natl. Acad. Sci. USA 105: 10619-10624 [Abstract] [Full Text]
Liu, F., Ng, S. K., Lu, Y., Low, W., Lai, J., Jedd, G. (2008). Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting. JCB 180: 325-339 [Abstract] [Full Text]
Becker, T., Pfannschmidt, S., Guiard, B., Stojanovski, D., Milenkovic, D., Kutik, S., Pfanner, N., Meisinger, C., Wiedemann, N. (2008). Biogenesis of the Mitochondrial TOM Complex: Mim1 PROMOTES INSERTION AND ASSEMBLY OF SIGNAL-ANCHORED RECEPTORS. J. Biol. Chem. 283: 120-127 [Abstract] [Full Text]
Kondo-Okamoto, N., Ohkuni, K., Kitagawa, K., McCaffery, J. M., Shaw, J. M., Okamoto, K. (2006). The Novel F-Box Protein Mfb1p Regulates Mitochondrial Connectivity and Exhibits Asymmetric Localization in Yeast. Mol. Biol. Cell 17: 3756-3767 [Abstract] [Full Text]
Meisinger, C., Wiedemann, N., Rissler, M., Strub, A., Milenkovic, D., Schonfisch, B., Muller, H., Kozjak, V., Pfanner, N. (2006). Mitochondrial Protein Sorting: DIFFERENTIATION OF beta-BARREL ASSEMBLY BY Tom7-MEDIATED SEGREGATION OF Mdm10. J. Biol. Chem. 281: 22819-22826 [Abstract] [Full Text]
Dimmer, K. S., Scorrano, L. (2006). (De)constructing Mitochondria: What For?. Physiology 21: 233-241 [Abstract] [Full Text]
Kucejova, B., Kucej, M., Petrezselyova, S., Abelovska, L., Tomaska, L. (2005). A Screen for Nigericin-Resistant Yeast Mutants Revealed Genes Controlling Mitochondrial Volume and Mitochondrial Cation Homeostasis. Genetics 171: 517-526 [Abstract] [Full Text]
Fagarasanu, M., Fagarasanu, A., Tam, Y. Y. C., Aitchison, J. D., Rachubinski, R. A. (2005). Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae. JCB 169: 765-775 [Abstract] [Full Text]
Dimmer, K. S., Jakobs, S., Vogel, F., Altmann, K., Westermann, B. (2005). Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast. JCB 168: 103-115 [Abstract] [Full Text]
Frederick, R. L., McCaffery, J. M., Cunningham, K. W., Okamoto, K., Shaw, J. M. (2004). Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway. JCB 167: 87-98 [Abstract] [Full Text]
Youngman, M. J., Hobbs, A. E. A., Burgess, S. M., Srinivasan, M., Jensen, R. E. (2004). Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids. JCB 164: 677-688 [Abstract] [Full Text]
Kondo-Okamoto, N., Shaw, J. M., Okamoto, K. (2003). Mmm1p Spans Both the Outer and Inner Mitochondrial Membranes and Contains Distinct Domains for Targeting and Foci Formation. J. Biol. Chem. 278: 48997-49005 [Abstract] [Full Text]
Meeusen, S., Nunnari, J. (2003). Evidence for a two membrane-spanning autonomous mitochondrial DNA replisome. JCB 163: 503-510 [Abstract] [Full Text]
Boldogh, I. R., Nowakowski, D. W., Yang, H.-C., Chung, H., Karmon, S., Royes, P., Pon, L. A. (2003). A Protein Complex Containing Mdm10p, Mdm12p, and Mmm1p Links Mitochondrial Membranes and DNA to the Cytoskeleton-based Segregation Machinery. Mol. Biol. Cell 14: 4618-4627 [Abstract] [Full Text]
Sesaki, H., Southard, S. M., Yaffe, M. P., Jensen, R. E. (2003). Mgm1p, a Dynamin-related GTPase, Is Essential for Fusion of the Mitochondrial Outer Membrane. Mol. Biol. Cell 14: 2342-2356 [Abstract] [Full Text]
Hanekamp, T., Thorsness, M. K., Rebbapragada, I., Fisher, E. M., Seebart, C., Darland, M. R., Coxbill, J. A., Updike, D. L., Thorsness, P. E. (2002). Maintenance of Mitochondrial Morphology Is Linked to Maintenance of the Mitochondrial Genome in Saccharomyces cerevisiae. Genetics 162: 1147-1156 [Abstract] [Full Text]
Versaw, W. K., Harrison, M. J. (2002). A Chloroplast Phosphate Transporter, PHT2;1, Influences Allocation of Phosphate within the Plant and Phosphate-Starvation Responses. Plant Cell 14: 1751-1766 [Abstract] [Full Text]
Mesa, R., Salomon, C., Roggero, M., Stahl, P. D., Mayorga, L. S. (2002). Rab22a affects the morphology and function of the endocytic pathway. J. Cell Sci. 114: 4041-4049 [Abstract] [Full Text]
Dimmer, K. S., Fritz, S., Fuchs, F., Messerschmitt, M., Weinbach, N., Neupert, W., Westermann, B. (2002). Genetic Basis of Mitochondrial Function and Morphology in Saccharomyces cerevisiae. Mol. Biol. Cell 13: 847-853 [Abstract] [Full Text]
Fuchs, F., Prokisch, H., Neupert, W., Westermann, B. (2002). Interaction of mitochondria with microtubules in the filamentous fungus Neurospora crassa. J. Cell Sci. 115: 1931-1937 [Abstract] [Full Text]
Hobbs, A. E. A., Srinivasan, M., McCaffery, J. M., Jensen, R. E. (2001). Mmm1p, a Mitochondrial Outer Membrane Protein, Is Connected to Mitochondrial DNA (Mtdna) Nucleoids and Required for Mtdna Stability. JCB 152: 401-410 [Abstract] [Full Text]
Kawai, A., Nishikawa, S.-i., Hirata, A., Endo, T. (2001). Loss of the mitochondrial Hsp70 functions causes aggregation of mitochondria in yeast cells. J. Cell Sci. 114: 3565-3574 [Abstract] [Full Text]
Lass-Flörl, C., Nagl, M., Speth, C., Ulmer, H., Dierich, M. P., Würzner, R. (2001). Studies of In Vitro Activities of Voriconazole and Itraconazole against Aspergillus Hyphae Using Viability Staining. Antimicrob. Agents Chemother. 45: 124-128 [Abstract] [Full Text]
Prokisch, H., Neupert, W., Westermann, B. (2000). Role of MMM1 in Maintaining Mitochondrial Morphology in Neurospora crassa. Mol. Biol. Cell 11: 2961-2971 [Abstract] [Full Text]
Contamine, V., Picard, M. (2000). Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast. Microbiol. Mol. Biol. Rev. 64: 281-315 [Abstract] [Full Text]
Sesaki, H., Jensen, R. E. (1999). Division versus Fusion: Dnm1p and Fzo1p Antagonistically Regulate Mitochondrial Shape. JCB 147: 699-706 [Abstract] [Full Text]
Fisk, H. A., Yaffe, M. P. (1999). A Role for Ubiquitination in Mitochondrial Inheritance in Saccharomyces cerevisiae. JCB 145: 1199-1208 [Abstract] [Full Text]
Meeusen, S., Tieu, Q., Wong, E., Weiss, E., Schieltz, D., Yates, J. R., Nunnari, J. (1999). Mgm101p Is a Novel Component of the Mitochondrial Nucleoid That Binds DNA and Is Required for the Repair of Oxidatively Damaged Mitochondrial DNA. JCB 145: 291-304 [Abstract] [Full Text]
Shepard, K. A., Yaffe, M. P. (1999). The Yeast Dynamin-like Protein, Mgm1p, Functions on the Mitochondrial Outer Membrane to Mediate Mitochondrial Inheritance. JCB 144: 711-720 [Abstract] [Full Text]
Pelloquin, L, Belenguer, P, Menon, Y, Gas, N, Ducommun, B (1999). Fission yeast Msp1 is a mitochondrial dynamin-related protein. J. Cell Sci. 112: 4151-4161 [Abstract]
Otsuga, D., Keegan, B. R., Brisch, E., Thatcher, J. W., Hermann, G. J., Bleazard, W., Shaw, J. M. (1998). The Dynamin-related GTPase, Dnm1p, Controls Mitochondrial Morphology in Yeast. JCB 143: 333-349 [Abstract] [Full Text]
Hermann, G. J., Thatcher, J. W., Mills, J. P., Hales, K. G., Fuller, M. T., Nunnari, J., Shaw, J. M. (1998). Mitochondrial Fusion in Yeast Requires the Transmembrane GTPase Fzo1p. JCB 143: 359-373 [Abstract] [Full Text]
Rinaldi, T., Ricci, C., Porro, D., Bolotin-Fukuhara, M., Frontali, L. (1998). A Mutation in a Novel Yeast Proteasomal Gene, RPN11/MPR1, Produces a Cell Cycle Arrest, Overreplication of Nuclear and Mitochondrial DNA, and an Altered Mitochondrial Morphology. Mol. Biol. Cell 9: 2917-2931 [Abstract] [Full Text]
Rapaport, D., Brunner, M., Neupert, W., Westermann, B. (1998). Fzo1p Is a Mitochondrial Outer Membrane Protein Essential for the Biogenesis of Functional Mitochondria in Saccharomyces cerevisiae. J. Biol. Chem. 273: 20150-20155 [Abstract] [Full Text]
Berger, K. H., Yaffe, M. P. (1998). Prohibitin Family Members Interact Genetically with Mitochondrial Inheritance Components in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 4043-4052 [Abstract] [Full Text]
Boldogh, I., Vojtov, N., Karmon, S., Pon, L. A. (1998). Interaction between Mitochondria and the Actin Cytoskeleton in Budding Yeast Requires Two Integral Mitochondrial Outer Membrane Proteins, Mmm1p and Mdm10p. JCB 141: 1371-1381 [Abstract] [Full Text]
Roeder, A. D., Hermann, G. J., Keegan, B. R., Thatcher, S. A., Shaw, J. M. (1998). Mitochondrial Inheritance Is Delayed in Saccharomyces cerevisiae Cells Lacking the Serine/Threonine Phosphatase PTC1. Mol. Biol. Cell 9: 917-930 [Abstract] [Full Text]
Fields, S., Conrad, M., Clarke, M (1998). The S. cerevisiae CLU1 and D. discoideum cluA genes are functional homologues that influence mitochondrial morphology and distribution. J. Cell Sci. 111: 1717-1727 [Abstract]
Kerscher, O., Holder, J., Srinivasan, M., Leung, R. S., Jensen, R. E. (1997). The Tim54p-Tim22p Complex Mediates Insertion of Proteins into the Mitochondrial Inner Membrane. JCB 139: 1663-1675 [Abstract] [Full Text]
Hermann, G. J., King, E. J., Shaw, J. M. (1997). The Yeast Gene, MDM20, Is Necessary for Mitochondrial Inheritance and Organization of the Actin Cytoskeleton. JCB 137: 141-153 [Abstract] [Full Text]
Berger, K. H., Sogo, L. F., Yaffe, M. P. (1997). Mdm12p, a Component Required for Mitochondrial Inheritance That Is Conserved between Budding and Fission Yeast. JCB 136: 545-553 [Abstract] [Full Text]
Odgren, P., Toukatly, G, Bangs, P., Gilmore, R, Fey, E. (1996). Molecular characterization of mitofilin (HMP), a mitochondria-associated protein with predicted coiled coil and intermembrane space targeting domains. J. Cell Sci. 109: 2253-2264 [Abstract]