Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins

doi:10.1083/jcb.127.1.95

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Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins

TH Smith, AM Kachinsky and JB Miller
Neuromuscular Laboratory, Massachusetts General Hospital, Charlestown 02129.

We show by immunohistology that distinct expression patterns of the four muscle regulatory factor (MRF) proteins identify subdomains of mouse somites. Myf-5 and MyoD are, at specific stages, each expressed in both myotome and dermatome cells. Myf-5 expression is initially restricted to dorsal cells in all somites, as is MyoD expression in neck somites. In trunk somites, however, MyoD is initially expressed in ventral cells. Myogenin and MRF4 are restricted to myotome cells, though the MRF4-expressing cells are initially less widely distributed than the myogenin-expressing cells, which are at all stages found throughout the myotome. All somitic myocytes express one or more MRFs. The transiently distinct expression patterns of the four MRF proteins identify dorsal and ventral subdomains of somites, and suggest that skeletal muscle cells in somites originate at multiple sites and via multiple molecular pathways.
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Denetclaw, W., Ordahl, C. (2000). The growth of the dermomyotome and formation of early myotome lineages in thoracolumbar somites of chicken embryos. Development 127: 893-905 [Abstract]
Brohmann, H, Jagla, K, Birchmeier, C (2000). The role of Lbx1 in migration of muscle precursor cells. Development 127: 437-445 [Abstract]
Dietrich, S, Abou-Rebyeh, F, Brohmann, H, Bladt, F, Sonnenberg-Riethmacher, E, Yamaai, T, Lumsden, A, Brand-Saberi, B, Birchmeier, C (1999). The role of SF/HGF and c-Met in the development of skeletal muscle. Development 126: 1621-1629 [Abstract]
Dominov, J. A., Dunn, J. J., Miller, J. B. (1998). Bcl-2 Expression Identifies an Early Stage of Myogenesis and Promotes Clonal Expansion of Muscle Cells. JCB 142: 537-544 [Abstract] [Full Text]
Dietrich, S, Schubert, F., Healy, C, Sharpe, P., Lumsden, A (1998). Specification of the hypaxial musculature. Development 125: 2235-2249 [Abstract]
Dietrich, S, Schubert, F., Lumsden, A (1997). Control of dorsoventral pattern in the chick paraxial mesoderm. Development 124: 3895-3908 [Abstract]
Wang, Y, Jaenisch, R (1997). Myogenin can substitute for Myf5 in promoting myogenesis but less efficiently. Development 124: 2507-2513 [Abstract]
Denetclaw, W., Christ, B, Ordahl, C. (1997). Location and growth of epaxial myotome precursor cells. Development 124: 1601-1610 [Abstract]
Moss, J. B., Olson, E. N., Schwartz, R. J. (1996). The Myogenic Regulatory Factor MRF4 Represses the Cardiac alpha -Actin Promoter through a Negative-acting N-terminal Protein Domain. J. Biol. Chem. 271: 31688-31694 [Abstract] [Full Text]
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Sporle, R, Gunther, T, Struwe, M, Schughart, K (1996). Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development 122: 79-86 [Abstract]
Zhang, W, Behringer, R R, Olson, E N (1995). Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies.. Genes Dev. 9: 1388-1399 [Abstract]
Patapoutian, A, Yoon, J., Miner, J., Wang, S, Stark, K, Wold, B (1995). Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development 121: 3347-3358 [Abstract]