ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure

doi:10.1083/jcb.127.2.303

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ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure

N Saitoh, IG Goldberg, ER Wood and WC Earnshaw
Department of Cell Biology and Anatomy, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.

Here, we describe the cloning and characterization of ScII, the second most abundant protein after topoisomerase II, of the chromosome scaffold fraction to be identified. ScII is structurally related to a protein, Smc1p, previously found to be required for accurate chromosome segregation in Saccharomyces cerevisiae. ScII and the other members of the emerging family of SMC1-like proteins are likely to be novel ATPases, with NTP-binding A and B sites separated by two lengthy regions predicted to form an alpha-helical coiled-coil. Analysis of the ScII B site predicted that ScII might use ATP by a mechanism similar to the bacterial recN DNA repair and recombination enzyme. ScII is a mitosis-specific scaffold protein that colocalizes with topoisomerase II in mitotic chromosomes. However, ScII appears not to be associated with the interphase nuclear matrix. ScII might thus play a role in mitotic processes such as chromosome condensation or sister chromatid disjunction, both of which have been previously shown to involve topoisomerase II.
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Varma, A., Wu, S., Guo, N., Liao, W., Lu, G., Li, A., Hu, Y., Bulmer, G., Kwon-Chung, K. J. (2006). Identification of a novel gene, URE2, that functionally complements a urease-negative clinical strain of Cryptococcus neoformans. Microbiology 152: 3723-3731 [Abstract] [Full Text]
Nishiyama, A., Dey, A., Miyazaki, J.-i., Ozato, K. (2006). Brd4 Is Required for Recovery from Antimicrotubule Drug-induced Mitotic Arrest: Preservation of Acetylated Chromatin. Mol. Biol. Cell 17: 814-823 [Abstract] [Full Text]
Savvidou, E., Cobbe, N., Steffensen, S., Cotterill, S., Heck, M. M. S. (2005). Drosophila CAP-D2 is required for condensin complex stability and resolution of sister chromatids. J. Cell Sci. 118: 2529-2543 [Abstract] [Full Text]
Uchiyama, S., Kobayashi, S., Takata, H., Ishihara, T., Hori, N., Higashi, T., Hayashihara, K., Sone, T., Higo, D., Nirasawa, T., Takao, T., Matsunaga, S., Fukui, K. (2005). Proteome Analysis of Human Metaphase Chromosomes. J. Biol. Chem. 280: 16994-17004 [Abstract] [Full Text]
Hirano, T. (2005). SMC proteins and chromosome mechanics: from bacteria to humans. Phil Trans R Soc B 360: 507-514 [Abstract] [Full Text]
Hirota, T., Gerlich, D., Koch, B., Ellenberg, J., Peters, J.-M. (2004). Distinct functions of condensin I and II in mitotic chromosome assembly. J. Cell Sci. 117: 6435-6445 [Abstract] [Full Text]
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Stray, J. E., Lindsley, J. E. (2003). Biochemical Analysis of the Yeast Condensin Smc2/4 Complex: AN ATPase THAT PROMOTES KNOTTING OF CIRCULAR DNA. J. Biol. Chem. 278: 26238-26248 [Abstract] [Full Text]
Uzbekov, R., Timirbulatova, E., Watrin, E., Cubizolles, F., Ogereau, D., Gulak, P., Legagneux, V., Polyakov, V. Ju., Le Guellec, K., Kireev, I. (2003). Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells. J. Cell Sci. 116: 1667-1678 [Abstract] [Full Text]
Belmont, A. S. (2002). Mitotic chromosome scaffold structure: New approaches to an old controversy. Proc. Natl. Acad. Sci. USA 99: 15855-15857 [Full Text]
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Poirier, M. G., Marko, J. F. (2002). From the Cover: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold. Proc. Natl. Acad. Sci. USA 99: 15393-15397 [Abstract] [Full Text]
Beasley, M., Xu, H., Warren, W., McKay, M. (2002). Conserved Disruptions in the Predicted Coiled-Coil Domains of Eukaryotic SMC Complexes: Implications for Structure and Function. Genome Res 12: 1201-1209 [Abstract] [Full Text]
Hirano, T. (2002). The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair. Genes Dev. 16: 399-414 [Full Text]
Politi, V., Perini, G., Trazzi, S., Pliss, A., Raska, I., Earnshaw, W. C., Valle, G. D. (2002). CENP-C binds the alpha-satellite DNA in vivo at specific centromere domains. J. Cell Sci. 115: 2317-2327 [Abstract] [Full Text]
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Hartman, T., Stead, K., Koshland, D., Guacci, V. (2000). Pds5p Is an Essential Chromosomal Protein Required for Both Sister Chromatid Cohesion and Condensation in Saccharomyces cerevisiae. JCB 151: 613-626 [Abstract] [Full Text]
Neuwald, A. F., Hirano, T. (2000). HEAT Repeats Associated with Condensins, Cohesins, and Other Complexes Involved in Chromosome-Related Functions. Genome Res 10: 1445-1452 [Abstract] [Full Text]
Schmiesing, J. A., Gregson, H. C., Zhou, S., Yokomori, K. (2000). A Human Condensin Complex Containing hCAP-C-hCAP-E and CNAP1, a Homolog of Xenopus XCAP-D2, Colocalizes with Phosphorylated Histone H3 during the Early Stage of Mitotic Chromosome Condensation. Mol. Cell. Biol. 20: 6996-7006 [Abstract] [Full Text]
Freeman, L., Aragon-Alcaide, L., Strunnikov, A. (2000). The Condensin Complex Governs Chromosome Condensation and Mitotic Transmission of Rdna. JCB 149: 811-824 [Abstract] [Full Text]
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Sutani, T., Yuasa, T., Tomonaga, T., Dohmae, N., Takio, K., Yanagida, M. (1999). Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev. 13: 2271-2283 [Abstract] [Full Text]
Ma, H., Siegel, A. J., Berezney, R. (1999). Association of Chromosome Territories with the Nuclear Matrix: Disruption of Human Chromosome Territories Correlates with the Release of a Subset of Nuclear Matrix Proteins. JCB 146: 531-542 [Abstract] [Full Text]
Skibbens, R. V., Corson, L. B., Koshland, D., Hieter, P. (1999). Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev. 13: 307-319 [Abstract] [Full Text]
Takagi, M, Matsuoka, Y, Kurihara, T, Yoneda, Y (1999). Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J. Cell Sci. 112: 2463-2472 [Abstract]
Hirano, T. (1999). SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates?. Genes Dev. 13: 11-19 [Full Text]
Graumann, P. L., Losick, R., Strunnikov, A. V. (1998). Subcellular Localization of Bacillus subtilis SMC, a Protein Involved in Chromosome Condensation and Segregation. J. Bacteriol. 180: 5749-5755 [Abstract] [Full Text]
Schmiesing, J. A., Ball, A. R. Jr., Gregson, H. C., Alderton, J. M., Zhou, S., Yokomori, K. (1998). Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics. Proc. Natl. Acad. Sci. USA 95: 12906-12911 [Abstract] [Full Text]
Melby, T. E., Ciampaglio, C. N., Briscoe, G., Erickson, H. P. (1998). The Symmetrical Structure of Structural Maintenance of Chromosomes (SMC) and MukB Proteins: Long, Antiparallel Coiled Coils, Folded at a Flexible Hinge. JCB 142: 1595-1604 [Abstract] [Full Text]
Akhmedov, A. T., Frei, C., Tsai-Pflugfelder, M., Kemper, B., Gasser, S. M., Jessberger, R. (1998). Structural Maintenance of Chromosomes Protein C-terminal Domains Bind Preferentially to DNA with Secondary Structure. J. Biol. Chem. 273: 24088-24094 [Abstract] [Full Text]
Connelly, J. C., Kirkham, L. A., Leach, D. R.�F. (1998). The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA. Proc. Natl. Acad. Sci. USA 95: 7969-7974 [Abstract] [Full Text]
Li, G., Sudlow, G., Belmont, A. S. (1998). Interphase Cell Cycle Dynamics of a Late-Replicating, Heterochromatic Homogeneously Staining Region: Precise Choreography of Condensation/Decondensation and Nuclear Positioning. JCB 140: 975-989 [Abstract] [Full Text]
Ferreira, J., Paolella, G., Ramos, C., Lamond, A. I. (1997). Spatial Organization of Large-Scale Chromatin Domains in the Nucleus: A Magnified View of Single Chromosome Territories. JCB 139: 1597-1610 [Abstract] [Full Text]
Wu, R.-R., Couchman, J. R. (1997). cDNA Cloning of the Basement Membrane Chondroitin Sulfate Proteoglycan Core Protein, Bamacan: A Five Domain Structure Including Coiled-Coil Motifs. JCB 136: 433-444 [Abstract] [Full Text]
Andreassen, P. R., Lacroix, F. B., Margolis, R. L. (1997). Chromosomes with Two Intact Axial Cores Are Induced by G2 Checkpoint Override: Evidence That DNA Decatenation Is not Required to Template the Chromosome Structure. JCB 136: 29-43 [Abstract] [Full Text]
Davis, T., Meyer, B. (1997). SDC-3 coordinates the assembly of a dosage compensation complex on the nematode X chromosome. Development 124: 1019-1031 [Abstract]
Chuang, P.-T., Lieb, J. D., Meyer, B. J. (1996). Sex-Specific Assembly of a Dosage Compensation Complex on the Nematode X Chromosome. Science 274: 1736-1739 [Abstract] [Full Text]
Hempel, K, Stratling, W. (1996). The chicken lysozyme gene 5' MAR and the Drosophila histone SAR are electroelutable from encapsulated and digested nuclei. J. Cell Sci. 109: 1459-1469 [Abstract]
Strunnikov, A V, Hogan, E, Koshland, D (1995). SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.. Genes Dev. 9: 587-599 [Abstract]
Cook, P. (1995). A chromomeric model for nuclear and chromosome structure. J. Cell Sci. 108: 2927-2935 [Abstract]
Michealis, S., Berkower, C. (1995). Sequence Comparison of Yeast ATP-binding Cassette Proteins. Cold Spring Harb Symp Quant Biol 60: 291-307 [Abstract]