Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses

doi:10.1083/jcb.127.4.1061

This Article

	Full Text (PDF, 1728K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Michel, R. N.
	Articles by Jasmin, B. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Michel, R. N.
	Articles by Jasmin, B. J.


Social Bookmarking

	What's this?

ARTICLES

Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses

RN Michel, CQ Vu, W Tetzlaff and BJ Jasmin
Department of Physiology, Faculty of Medicine, University of Ottawa, Ontario, Canada.

We examined the role of innervation on acetylcholinesterase (AChE) gene expression within mammalian skeletal muscle fibers. First, we showed the selective accumulation of AChE mRNAs within the junctional vs extrajunctional sarcoplasm of adult muscle fibers using a quantitative reverse transcription PCR assay and demonstrated by in situ hybridization experiments that AChE transcripts are concentrated immediately beneath the postsynaptic membrane of the neuromuscular junction. Next, we determined the influence of nerve-evoked activity vs putative trophic factors on the synaptic accumulation of AChE mRNA levels in muscle fibers paralyzed by either surgical denervation or selective blockage of nerve action potentials with chronic superfusion of tetrodotoxin. Our results indicated that muscle paralysis leads to a marked decrease in AChE transcripts from the postsynaptic sarcoplasm, yet the extent of this decrease is less pronounced after tetrodotoxin inactivation than after denervation. These results suggest that although nerve-evoked activity per se appears a key regulator of AChE mRNA levels, the integrity of the synaptic structure or the release of putative trophic factors contribute to maintaining the synaptic accumulation of AChE transcripts at adult neuromuscular synapses. Furthermore, the pronounced downregulation of AChE transcripts in paralyzed muscles stands in sharp contrast to the well-documented increase in nicotinic acetylcholine receptor mRNAs under these conditions, and indicates that expression of the genes encoding these two synaptic proteins are subjected to different regulatory mechanisms in adult muscle fibers in vivo.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ruiz, C. A., Rotundo, R. L. (2009). Limiting Role of Protein Disulfide Isomerase in the Expression of Collagen-tailed Acetylcholinesterase Forms in Muscle. J. Biol. Chem. 284: 31753-31763 [Abstract] [Full Text]
Chakkalakal, J. V., Miura, P., Belanger, G., Michel, R. N., Jasmin, B. J. (2008). Modulation of utrophin A mRNA stability in fast versus slow muscles via an AU-rich element and calcineurin signaling. Nucleic Acids Res 36: 826-838 [Abstract] [Full Text]
Martinez-Pena y Valenzuela, I., Akaaboune, M. (2007). Acetylcholinesterase Mobility and Stability at the Neuromuscular Junction of Living Mice. Mol. Biol. Cell 18: 2904-2911 [Abstract] [Full Text]
Pregelj, P., Trinkaus, M., Zupan, D., Trontelj, J. J., Sketelj, J. (2007). The Role of Muscle Activation Pattern and Calcineurin in Acetylcholinesterase Regulation in Rat Skeletal Muscles. J. Neurosci. 27: 1106-1113 [Abstract] [Full Text]
Deschenes-Furry, J., Mousavi, K., Bolognani, F., Neve, R. L., Parks, R. J., Perrone-Bizzozero, N. I., Jasmin, B. J. (2007). The RNA-Binding Protein HuD Binds Acetylcholinesterase mRNA in Neurons and Regulates its Expression after Axotomy. J. Neurosci. 27: 665-675 [Abstract] [Full Text]
Mousavi, K., Jasmin, B. J. (2006). BDNF Is Expressed in Skeletal Muscle Satellite Cells and Inhibits Myogenic Differentiation. J. Neurosci. 26: 5739-5749 [Abstract] [Full Text]
Angus, L. M., Chakkalakal, J. V., Mejat, A., Eibl, J. K., Belanger, G., Megeney, L. A., Chin, E. R., Schaeffer, L., Michel, R. N., Jasmin, B. J. (2005). Calcineurin-NFAT signaling, together with GABP and peroxisome PGC-1{alpha}, drives utrophin gene expression at the neuromuscular junction. Am. J. Physiol. Cell Physiol. 289: C908-C917 [Abstract] [Full Text]
Miura, P., Thompson, J., Chakkalakal, J. V., Holcik, M., Jasmin, B. J. (2005). The Utrophin A 5'-Untranslated Region Confers Internal Ribosome Entry Site-mediated Translational Control during Regeneration of Skeletal Muscle Fibers. J. Biol. Chem. 280: 32997-33005 [Abstract] [Full Text]
Deschenes-Furry, J., Belanger, G., Mwanjewe, J., Lunde, J. A., Parks, R. J., Perrone-Bizzozero, N., Jasmin, B. J. (2005). The RNA-binding Protein HuR Binds to Acetylcholinesterase Transcripts and Regulates Their Expression in Differentiating Skeletal Muscle Cells. J. Biol. Chem. 280: 25361-25368 [Abstract] [Full Text]
Lai, K.-O., Chen, Y., Po, H.-M., Lok, K.-C., Gong, K., Ip, N. Y. (2004). Identification of the Jak/Stat Proteins as Novel Downstream Targets of EphA4 Signaling in Muscle: IMPLICATIONS IN THE REGULATION OF ACETYLCHOLINESTERASE EXPRESSION. J. Biol. Chem. 279: 13383-13392 [Abstract] [Full Text]
Chakkalakal, J. V., Harrison, M.-A., Carbonetto, S., Chin, E., Michel, R. N., Jasmin, B. J. (2004). Stimulation of calcineurin signaling attenuates the dystrophic pathology in mdx mice. Hum Mol Genet 13: 379-388 [Abstract] [Full Text]
Jiang, J. X. S., Choi, R. C. Y., Siow, N. L., Lee, H. H. C., Wan, D. C. C., Tsim, K. W. K. (2003). Muscle Induces Neuronal Expression of Acetylcholinesterase in Neuron-Muscle Co-culture: TRANSCRIPTIONAL REGULATION MEDIATED BY cAMP-DEPENDENT SIGNALING. J. Biol. Chem. 278: 45435-45444 [Abstract] [Full Text]
Chakkalakal, J. V., Stocksley, M. A., Harrison, M.-A., Angus, L. M., Deschenes-Furry, J., St-Pierre, S., Megeney, L. A., Chin, E. R., Michel, R. N., Jasmin, B. J. (2003). Expression of utrophin A mRNA correlates with the oxidative capacity of skeletal muscle fiber types and is regulated by calcineurin/NFAT signaling. Proc. Natl. Acad. Sci. USA 100: 7791-7796 [Abstract] [Full Text]
Deschenes-Furry, J., Belanger, G., Perrone-Bizzozero, N., Jasmin, B. J. (2003). Post-transcriptional Regulation of Acetylcholinesterase mRNAs in Nerve Growth Factor-treated PC12 Cells by the RNA-binding Protein HuD. J. Biol. Chem. 278: 5710-5717 [Abstract] [Full Text]
Choi, R. C. Y., Man, M. L. S., Ling, K. K. Y., Ip, N. Y., Simon, J., Barnard, E. A., Tsim, K. W. K. (2001). Expression of the P2Y1 Nucleotide Receptor in Chick Muscle: Its Functional Role in the Regulation of Acetylcholinesterase and Acetylcholine Receptor. J. Neurosci. 21: 9224-9234 [Abstract] [Full Text]
Gramolini, A. O., Belanger, G., Thompson, J. M., Chakkalakal, J. V., Jasmin, B. J. (2001). Increased expression of utrophin in a slow vs. a fast muscle involves posttranscriptional events. Am. J. Physiol. Cell Physiol. 281: C1300-C1309 [Abstract] [Full Text]
Matar, W., Lunde, J. A., Jasmin, B. J., Renaud, J.-M. (2001). Denervation enhances the physiological effects of the KATP channel during fatigue in EDL and soleus muscle. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281: R56-R65 [Abstract] [Full Text]
Rossi, S. G., Vazquez, A. E., Rotundo, R. L. (2000). Local Control of Acetylcholinesterase Gene Expression in Multinucleated Skeletal Muscle Fibers: Individual Nuclei Respond to Signals from the Overlying Plasma Membrane. J. Neurosci. 20: 919-928 [Abstract] [Full Text]
Boudreau-Lariviere, C., Parry, D. J., Jasmin, B. J. (2000). Myotubes originating from single fast and slow satellite cells display similar patterns of AChE expression. Am. J. Physiol. Regul. Integr. Comp. Physiol. 278: R140-R148 [Abstract] [Full Text]
Legay, C., Mankal, F. A., Massoulie, J., Jasmin, B. J. (1999). Stability and Secretion of Acetylcholinesterase Forms in Skeletal Muscle Cells. J. Neurosci. 19: 8252-8259 [Abstract] [Full Text]
Dunn, S. E., Burns, J. L., Michel, R. N. (1999). Calcineurin Is Required for Skeletal Muscle Hypertrophy. J. Biol. Chem. 274: 21908-21912 [Abstract] [Full Text]
Chan, R. Y.�Y., Boudreau-Lariviere, C., Angus, L. M., Mankal, F. A., Jasmin, B. J. (1999). An intronic enhancer containing an N-box motif is required for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers. Proc. Natl. Acad. Sci. USA 96: 4627-4632 [Abstract] [Full Text]
Roczniak, A., Zimpelmann, J., Burns, K. D. (1998). Effect of dietary salt on neuronal nitric oxide synthase in the inner medullary collecting duct. Am. J. Physiol. Renal Physiol. 275: F46-F54 [Abstract] [Full Text]
Chan, R. Y.�Y., Adatia, F. A., Krupa, A. M., Jasmin, B. J. (1998). Increased Expression of Acetylcholinesterase T and R Transcripts during Hematopoietic Differentiation Is Accompanied by Parallel Elevations in the Levels of Their Respective Molecular Forms. J. Biol. Chem. 273: 9727-9733 [Abstract] [Full Text]
Sketelj, J., Crne-Finderle, N., Strukelj, B., Trontelj, J. V., Pette, D. (1998). Acetylcholinesterase mRNA Level and Synaptic Activity in Rat Muscles Depend on Nerve-Induced Pattern of Muscle Activation. J. Neurosci. 18: 1944-1952 [Abstract] [Full Text]
Hubatsch, D. A., Jasmin, B. J. (1997). Mechanical stimulation increases expression of acetylcholinesterase in cultured myotubes. Am. J. Physiol. Cell Physiol. 273: C2002-C2009 [Abstract] [Full Text]
Gramolini, A. O., Dennis, C. L., Tinsley, J. M., Robertson, G. S., Cartaud, J., Davies, K. E., Jasmin, B. J. (1997). Local Transcriptional Control of Utrophin Expression at the Neuromuscular Synapse. J. Biol. Chem. 272: 8117-8120 [Abstract] [Full Text]
Ray, A., Kyselovic, J., Leddy, J. J., Wigle, J. T., Jasmin, B. J., Tuana, B. S. (1995). Regulation of Dihydropyridine and Ryanodine Receptor Gene Expression in Skeletal Muscle. J. Biol. Chem. 270: 25837-25844 [Abstract] [Full Text]
Angus, L. M., Chan, R. Y. Y., Jasmin, B. J. (2001). Role of Intronic E- and N-box Motifs in the Transcriptional Induction of the Acetylcholinesterase Gene during Myogenic Differentiation. J. Biol. Chem. 276: 17603-17609 [Abstract] [Full Text]